FastQCFastQC Report
Fri 27 May 2016
SRR2139909_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139909_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22452201
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA328950.051.2125661
GTATCAA624250.038.3980181
TCAACGC783050.030.0454734
ATCAACG785150.029.9764463
CAACGCA795450.029.570545
TATCAAC812700.029.1624852
AACGCAG817950.028.8086996
ACGCAGA931700.025.2412667
CGCAGAG942000.024.9332878
GCAGAGT1087100.021.2804999
AGAGTAC985600.019.2153710-11
GTACATG731700.019.212251
GAGTACT645700.019.06973612-13
TACATGG730300.018.9433562
CAGAGTA1050000.018.71345910-11
ACATGGG728800.018.48523
AGTACTT695200.017.45189912-13
CTTATAC124600.017.4456331
GTACTTT717450.017.09129114-15
CATGGGG485650.016.3245644
Produced by FastQC (version 0.11.5)