##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139909_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22452201
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.822868457306257	33.0	31.0	34.0	30.0	34.0
2	31.85865145247898	33.0	31.0	34.0	29.0	34.0
3	31.93814009593091	34.0	31.0	34.0	29.0	34.0
4	35.4514377009185	37.0	35.0	37.0	33.0	37.0
5	35.28907317371691	37.0	35.0	37.0	32.0	37.0
6	35.35149841211559	37.0	35.0	37.0	32.0	37.0
7	35.25750303945702	37.0	35.0	37.0	32.0	37.0
8	35.26368800101157	37.0	35.0	37.0	32.0	37.0
9	36.84666336275895	39.0	37.0	39.0	32.0	39.0
10-11	36.80725564500335	39.0	37.0	39.0	32.0	39.0
12-13	36.778389477272185	39.0	37.0	39.0	32.0	39.0
14-15	38.02154356715406	40.0	38.0	41.0	32.0	41.0
16-17	38.04768120506314	40.0	38.0	41.0	32.0	41.0
18-19	38.083938340833484	40.0	38.0	41.0	32.0	41.0
20-21	38.12606470117535	40.0	38.0	41.0	32.0	41.0
22-23	38.050365528443834	40.0	38.0	41.0	32.0	41.0
24-25	38.06253118396979	40.0	38.0	41.0	32.0	41.0
26-27	37.99177340717506	40.0	38.0	41.0	32.0	41.0
28-29	37.932097225632305	40.0	37.0	41.0	32.0	41.0
30-31	37.87263813095656	40.0	37.0	41.0	32.0	41.0
32-33	37.7676458757055	40.0	37.0	41.0	32.0	41.0
34-35	37.682998253878644	40.0	37.0	41.0	31.0	41.0
36-37	37.63175538781566	40.0	37.0	41.0	31.0	41.0
38-39	37.61203455254462	40.0	37.0	41.0	31.0	41.0
40-41	37.56858003392935	40.0	37.0	41.0	31.0	41.0
42-43	37.52799083555935	40.0	36.0	41.0	31.0	41.0
44-45	37.46586227938256	40.0	36.0	41.0	31.0	41.0
46-47	37.4223351751298	40.0	36.0	41.0	31.0	41.0
48-49	37.35691902693719	40.0	36.0	41.0	31.0	41.0
50-51	37.270737375084806	40.0	36.0	41.0	31.0	41.0
52-53	37.189139948637006	39.5	36.0	41.0	31.0	41.0
54-55	37.22388539421067	39.0	36.0	41.0	31.0	41.0
56-57	37.29291049294818	39.0	36.0	41.0	31.0	41.0
58-59	37.194132150410915	39.0	35.0	41.0	31.0	41.0
60-61	37.058174391106476	39.0	35.0	41.0	31.0	41.0
62-63	36.86802355929412	39.0	35.0	41.0	31.0	41.0
64-65	36.660002058976715	38.0	35.0	41.0	31.0	41.0
66-67	36.411627257270396	38.0	35.0	40.0	30.0	41.0
68-69	36.168421112499786	37.0	35.0	40.0	30.0	41.0
70-71	35.88849984579742	37.0	34.5	39.5	30.0	41.0
72-73	35.62278019222174	36.0	34.0	39.0	30.0	41.0
74-75	35.339694755414826	36.0	34.0	39.0	30.0	41.0
76-77	35.038647791863646	35.5	34.0	38.5	30.0	40.0
78-79	34.73271663494195	35.0	34.0	37.0	30.0	39.0
80-81	34.46566070740895	35.0	34.0	37.0	29.0	39.0
82-83	34.22233376453748	35.0	34.0	36.0	29.0	39.0
84-85	34.007099943165464	35.0	34.0	36.0	29.0	37.0
86-87	33.80117738739804	35.0	34.0	36.0	29.0	37.0
88-89	33.668455929142766	35.0	33.5	35.0	29.0	36.5
90-91	33.56085456407267	35.0	33.0	35.0	29.0	36.0
92-93	33.48602738500436	35.0	33.0	35.0	29.0	36.0
94-95	33.542961422445075	35.0	33.5	35.0	29.5	36.0
96-97	33.709749021753595	35.0	34.0	35.0	30.0	36.0
98-99	33.78523688139235	35.0	34.0	35.0	30.5	35.5
100	33.12233073110248	35.0	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	12.0
15	24.0
16	54.0
17	236.0
18	1933.0
19	9919.0
20	30275.0
21	66872.0
22	111006.0
23	153977.0
24	194007.0
25	233151.0
26	268688.0
27	303803.0
28	345044.0
29	393986.0
30	457135.0
31	540076.0
32	651495.0
33	808671.0
34	1058549.0
35	1439504.0
36	2186422.0
37	3968675.0
38	5702658.0
39	3349870.0
40	176156.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.55488368378673	20.95984264527117	14.316774555866482	24.168499115075623
2	14.531637147879733	22.52417344011198	40.169853740620255	22.774335671388034
3	16.897429958576453	28.006271435570802	31.571021335352544	23.5252772705002
4	12.093451463391878	19.566906897831853	39.713040932657556	28.626600706118715
5	12.35440322234176	38.80993424647278	36.05809520668642	12.777567324499051
6	27.83483017656261	39.92347443830575	19.42813564638182	12.813559738749824
7	24.591188307420385	33.89057519848923	24.44767017403122	17.07056632005916
8	24.240957961235125	36.084207762078535	22.061490504575456	17.61334377211088
9	25.83068513995061	15.935788453453728	21.346240278024787	36.88728612857087
10-11	23.332919044992405	26.927794218786953	29.974837829052188	19.76444890716845
12-13	24.71189419213325	24.8811406542121	29.62130873063127	20.78565642302338
14-15	22.022919272573603	25.896148421294736	27.404882800773695	24.676049505357962
16-17	21.18740172806066	28.949513401840075	27.91754961323683	21.94553525686243
18-19	21.252562839079427	28.031291968417637	29.838103262355574	20.87804193014736
20-21	22.31865068341967	27.106337005362736	29.567858432943357	21.00715387827424
22-23	21.97031102391423	27.124738228002364	29.43032721708955	21.47462353099386
24-25	21.656931375465547	27.312202011647507	29.501456608607796	21.52941000427915
26-27	21.66300058574705	27.441938388462596	29.365782237968762	21.529278787821596
28-29	21.631074914596393	27.37144673697284	29.43955357071063	21.557924777720142
30-31	21.896439607861247	27.24384580649126	29.310230198845744	21.549484386801744
32-33	21.66078475062353	27.430038268411444	29.297315753971702	21.61186122699332
34-35	21.821343184788482	27.437721621933083	29.21632223991704	21.52461295336139
36-37	21.828131294428037	27.408195651588102	29.152695156476177	21.61097789750768
38-39	21.708775058591247	27.500084005891672	29.136772853470926	21.65436808204615
40-41	21.97770417490339	27.377773139244876	29.005741314879664	21.638781370972072
42-43	21.902693191164182	27.484212460370255	28.79582254907544	21.817271799390117
44-45	21.989032362704172	27.66534389158218	28.488981605956642	21.856642139757007
46-47	22.124701929947793	27.641039796744433	28.100918627493066	22.13333964581471
48-49	22.106418304180334	27.758121432817173	28.04039558201328	22.095064680989214
50-51	22.079727832966714	27.882712672811387	27.864972377226486	22.17258711699541
52-53	22.2102375981346	27.82194056037481	27.833855775966033	22.13396606552456
54-55	22.171966673417693	27.726712602989938	27.91270801975011	22.18861270384226
56-57	22.19025974574823	27.839012132182724	27.83386393027218	22.13686419179687
58-59	22.13780891747572	27.919666804021226	27.864706652880056	22.077817625623
60-61	22.270138600163236	27.923472829013562	27.74726624655524	22.059122324267964
62-63	22.18362525933054	28.033528746297883	27.738333053152388	22.044512941219192
64-65	22.183717131716026	27.958894335708635	27.682956762882267	22.174431769693072
66-67	22.241186897547962	27.98256298480672	27.592319430670653	22.18393068697467
68-69	22.17136483671563	27.884438290259773	27.707083603683657	22.237113269340945
70-71	22.24873855780915	27.63664505188881	27.770282425608123	22.34433396469391
72-73	22.258611056190762	27.714258618191543	27.698182768723477	22.32894755689421
74-75	22.095544036121925	27.832870195573197	27.72191218753314	22.349673580771743
76-77	22.246745671888608	27.745988940939192	27.69497532113784	22.312290066034357
78-79	22.20643078833295	27.82080713061776	27.62891232265029	22.343849758399
80-81	22.235525048044956	27.819322019915806	27.606235814644325	22.338917117394914
82-83	22.256343945629848	27.778171865167085	27.691597840321574	22.273886348881494
84-85	22.289180725466206	27.763014116366897	27.64194468459879	22.30586047356811
86-87	22.14196730215111	27.80516797034162	27.730343125969537	22.322521601537733
88-89	22.19325676445037	27.786717761599462	27.670300389037482	22.349725084912684
90-91	22.262974453719206	27.799622594495716	27.696450247150818	22.24095270463426
92-93	22.301914603238117	27.789535985458123	27.65307861844005	22.25547079286371
94-95	22.106049563928913	27.836199532627294	27.715560310998445	22.342190592445345
96-97	22.179793036209247	27.98209081027349	27.773192252714303	22.064923900802967
98-99	23.24282585900782	29.92798187234815	27.933028316393703	18.896163952250323
100	22.258310866231675	30.909186625347207	21.72152371898525	25.11097878943587
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5286.0
1	5493.5
2	8586.0
3	11598.0
4	14603.0
5	24932.0
6	33904.5
7	38810.5
8	40649.5
9	37195.0
10	30944.0
11	24301.0
12	25038.0
13	35544.5
14	62581.5
15	102021.5
16	135470.5
17	154876.0
18	158966.5
19	149535.0
20	132993.0
21	117230.0
22	108657.5
23	114136.5
24	133968.5
25	166585.5
26	210110.0
27	258188.5
28	306646.5
29	359014.5
30	411909.5
31	464538.5
32	522541.5
33	576831.0
34	627045.5
35	674022.5
36	722939.5
37	770411.0
38	801604.5
39	826750.0
40	855949.0
41	880164.0
42	895654.5
43	910096.0
44	925631.0
45	941087.5
46	949349.5
47	950319.5
48	942306.0
49	921368.0
50	894784.5
51	857730.0
52	812296.5
53	765197.0
54	716037.0
55	663613.5
56	610179.5
57	553366.5
58	493966.5
59	433250.5
60	365127.5
61	297258.5
62	236363.5
63	184271.0
64	141247.0
65	107062.5
66	81522.0
67	63233.5
68	49166.5
69	37523.0
70	28364.5
71	21407.0
72	16151.5
73	12123.5
74	8955.5
75	6573.5
76	4664.5
77	3238.5
78	2394.5
79	1819.5
80	1381.0
81	1030.0
82	802.0
83	578.5
84	394.5
85	292.0
86	237.0
87	195.0
88	130.0
89	78.0
90	57.5
91	45.5
92	34.5
93	19.5
94	9.5
95	6.0
96	6.0
97	6.5
98	6.0
99	5.0
100	8.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.01233286660848974
3	0.005349141493967562
4	0.13321188421571675
5	0.06173559554361731
6	0.0027525141076369304
7	0.04028112878554757
8	8.462422013770499E-5
9	4.5429844494978465E-4
10-11	1.9819883137515114E-4
12-13	1.4697890866022446E-4
14-15	2.2269531615185524E-5
16-17	6.836746205861955E-4
18-19	2.226953161518552E-4
20-21	0.004272193314407465
22-23	0.0507166012140529
24-25	7.079755590756805E-4
26-27	6.708559083800458E-5
28-29	0.0019463618510210292
30-31	0.0033701494657920176
32-33	0.0033729122923388923
34-35	0.01828179951727543
36-37	0.007735632024215939
38-39	0.0036759809982453715
40-41	0.012045507845600024
42-43	0.003234620437871336
44-45	0.009471817059155771
46-47	0.00268051671530099
48-49	7.076473991151481E-4
50-51	0.03783696954897345
52-53	5.8563384541970154E-5
54-55	4.917584117796596E-5
56-57	0.006672140035628534
58-59	3.47404932640183E-4
60-61	4.4876494718175234E-4
62-63	5.441693764309715E-4
64-65	0.004205289515720911
66-67	2.0696219799235283E-4
68-69	6.267187598781648E-4
70-71	4.7466732149660705E-5
72-73	9.345098430643808E-4
74-75	5.373398972267592E-6
76-77	2.9826174680842306E-5
78-79	8.21408827302819E-6
80-81	5.671274452668069E-4
82-83	0.0
84-85	1.4125800936444547E-5
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	3.300907914721944E-5
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	11058.0
20-21	22290.0
22-23	24834.0
24-25	26979.0
26-27	32778.0
28-29	47453.0
30-31	74580.0
32-33	86086.0
34-35	105878.0
36-37	134810.0
38-39	167586.0
40-41	170211.0
42-43	195755.0
44-45	233968.0
46-47	224321.0
48-49	194148.0
50-51	169918.0
52-53	155155.0
54-55	159464.0
56-57	167338.0
58-59	171268.0
60-61	172441.0
62-63	163593.0
64-65	169095.0
66-67	178714.0
68-69	183890.0
70-71	185789.0
72-73	170703.0
74-75	168965.0
76-77	175734.0
78-79	185827.0
80-81	192106.0
82-83	186026.0
84-85	182943.0
86-87	190165.0
88-89	203307.0
90-91	218647.0
92-93	227070.0
94-95	258848.0
96-97	713393.0
98-99	1388440.0
100-101	1.4360627E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.10831595139774
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.84841056401825	71.25236639854499
2	7.881058215064564	12.78438719477631
3	2.1712881240075586	5.283285695505683
4	0.8838497836586843	2.8675027002625244
5	0.452788262084232	1.836244671010609
6	0.25538675615441586	1.2428393824784958
7	0.14688548853164324	0.8339544228749952
8	0.09496600111503742	0.6162025938463397
9	0.06235758628098639	0.45519469290403114
>10	0.19799284105149803	2.3978478514892934
>50	0.003864783687480005	0.2114771532718134
>100	0.001030648915268088	0.13412969132977154
>500	8.807233447701552E-5	0.045337923279375116
>1k	3.2873095766844475E-5	0.03922962842575294
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.453906323037104E-6	0.0	0.0	0.0	4.453906323037104E-6
2	4.453906323037104E-6	8.017031381466788E-5	0.0	0.0	4.453906323037104E-6
3	4.453906323037104E-6	8.462422013770499E-5	0.0	0.0	4.453906323037104E-6
4	4.453906323037104E-6	1.3361718969111312E-4	0.0	8.907812646074209E-6	4.453906323037104E-6
5	1.3361718969111314E-5	1.4697890866022446E-4	0.0	1.7815625292148417E-5	4.453906323037104E-6
6	1.3361718969111314E-5	1.4697890866022446E-4	0.0	1.7815625292148417E-5	4.453906323037104E-6
7	1.3361718969111314E-5	1.5143281498326155E-4	0.0	2.2269531615185524E-5	4.453906323037104E-6
8	1.3361718969111314E-5	1.6479453395237286E-4	0.0	2.2269531615185524E-5	1.7815625292148417E-5
9	1.3361718969111314E-5	3.073195362895602E-4	0.0	4.008515690733394E-5	1.7815625292148417E-5
10-11	1.7815625292148417E-5	3.0954648945107874E-4	0.0	5.12199227149267E-5	1.7815625292148417E-5
12-13	3.1177344261259734E-5	3.117734426125973E-4	0.0	6.012773536100091E-5	1.7815625292148417E-5
14-15	3.785820374581539E-5	4.342558664961177E-4	0.0	6.903554800707511E-5	2.4496484776704074E-5
16-17	4.67660163918896E-5	4.431636791421919E-4	0.0	7.126250116859367E-5	3.5631250584296834E-5
18-19	4.899296955340815E-5	4.899296955340815E-4	0.0	7.794336065314932E-5	3.785820374581539E-5
20-21	4.899296955340815E-5	5.366957119259711E-4	0.0	9.35320327837792E-5	4.008515690733394E-5
22-23	6.903554800707512E-5	5.879156346408978E-4	0.0	1.2693633020655748E-4	4.67660163918896E-5
24-25	8.685117329922354E-5	6.458164168403801E-4	0.0	1.5588672130629864E-4	6.235468852251947E-5
26-27	8.90781264607421E-5	6.591781358094914E-4	0.0	2.1601445666729957E-4	6.235468852251947E-5
28-29	9.353203278377919E-5	6.591781358094914E-4	0.0	3.941707095887838E-4	6.235468852251947E-5
30-31	1.0466679859137196E-4	7.371214964626408E-4	0.0	9.88767203714237E-4	6.235468852251947E-5
32-33	1.2025547072200182E-4	7.46029309108715E-4	0.0	0.0022314070678415895	6.235468852251947E-5
34-35	1.3139023652959458E-4	8.596039203461612E-4	0.0	0.003741281311351168	6.903554800707511E-5
36-37	1.3807109601415024E-4	9.709515784220888E-4	0.0	0.006226561039605873	7.571640749163078E-5
38-39	1.3807109601415024E-4	9.932211100372743E-4	0.0	0.010130409931747894	7.794336065314932E-5
40-41	1.3807109601415024E-4	0.0010822992364980164	0.0	0.014611039692723222	8.017031381466788E-5
42-43	1.3807109601415024E-4	0.0011157035339207947	0.0	0.01861064757081054	8.462422013770499E-5
44-45	1.5143281498326155E-4	0.0012114625198660924	0.0	0.02304451131539398	9.130507962226064E-5
46-47	1.625675807908543E-4	0.001278271114711649	0.0	0.0278124180342052	1.0021289226833484E-4
48-49	1.6479453395237286E-4	0.0012849519741962045	0.0	0.03275625405277638	1.1580156439896471E-4
50-51	1.6479453395237286E-4	0.001472016039763763	0.0	0.03786711155846146	1.1802851756048327E-4
52-53	1.6924844027540998E-4	0.0014876047118943928	0.0	0.04270405382527975	1.402980491756688E-4
54-55	1.6924844027540998E-4	0.0014987394777019856	0.0	0.04717354882044749	1.6034062762933576E-4
56-57	1.6924844027540998E-4	0.0015187820561556526	0.0	0.05130009302874137	1.625675807908543E-4
58-59	1.7147539343692852E-4	0.0015210090093171712	0.0	0.055800765368170364	1.6479453395237286E-4
60-61	1.7370234659844707E-4	0.0015343707282862826	0.0	0.06023685606591532	1.6924844027540998E-4
62-63	1.7370234659844707E-4	0.0015789097915166535	0.0	0.06470635106108305	1.781562529214842E-4
64-65	1.7370234659844707E-4	0.0016835765901080256	0.0	0.06876608667453138	1.8038320608300274E-4
66-67	1.7370234659844707E-4	0.0016991652622386554	0.0	0.07278796408423388	1.8261015924452128E-4
68-69	1.7370234659844707E-4	0.0017659738570842119	0.0	0.07682097625974398	1.8261015924452128E-4
70-71	1.7370234659844707E-4	0.001810512920314583	0.0	0.0805221724141878	1.8483711240603983E-4
72-73	1.7370234659844707E-4	0.0018216476861221758	0.0	0.08324128222440197	2.2269531615185523E-4
74-75	1.7370234659844707E-4	0.0018595058898679912	0.0	0.08549718577702026	2.2269531615185523E-4
76-77	1.7370234659844707E-4	0.001990896126397586	0.0	0.08776645104860767	2.2269531615185523E-4
78-79	1.781562529214842E-4	0.0020398890959509937	0.0	0.08922510536940231	2.2269531615185523E-4
80-81	1.781562529214842E-4	0.0020398890959509937	0.0	0.08970612725229032	2.293761756364109E-4
82-83	1.781562529214842E-4	0.0020421160491125123	0.0	0.08997336163167255	2.560996135746335E-4
84-85	1.781562529214842E-4	0.002053250814920105	0.0	0.09005575889864872	2.5832656673615206E-4
86-87	1.781562529214842E-4	0.0021111515971195876	0.0	0.09009361710239455	2.605535198976706E-4
88	1.781562529214842E-4	0.0022314070678415895	0.0	0.09009361710239455	2.6723437938222625E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	32895	0.0	51.212566	1
GTATCAA	62425	0.0	38.398018	1
TCAACGC	78305	0.0	30.045473	4
ATCAACG	78515	0.0	29.976446	3
CAACGCA	79545	0.0	29.57054	5
TATCAAC	81270	0.0	29.162485	2
AACGCAG	81795	0.0	28.808699	6
ACGCAGA	93170	0.0	25.241266	7
CGCAGAG	94200	0.0	24.933287	8
GCAGAGT	108710	0.0	21.280499	9
AGAGTAC	98560	0.0	19.21537	10-11
GTACATG	73170	0.0	19.21225	1
GAGTACT	64570	0.0	19.069736	12-13
TACATGG	73030	0.0	18.943356	2
CAGAGTA	105000	0.0	18.713459	10-11
ACATGGG	72880	0.0	18.4852	3
AGTACTT	69520	0.0	17.451899	12-13
CTTATAC	12460	0.0	17.445633	1
GTACTTT	71745	0.0	17.091291	14-15
CATGGGG	48565	0.0	16.324564	4
>>END_MODULE