FastQCFastQC Report
Fri 27 May 2016
SRR2139908_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139908_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16685221
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA237500.049.6811261
GTATCAA449200.037.9437181
TCAACGC566650.029.582164
ATCAACG568150.029.5197163
CAACGCA577600.029.0157555
TATCAAC586950.028.7099442
AACGCAG590000.028.4241946
ACGCAGA670100.024.9823237
CGCAGAG677900.024.6856238
GCAGAGT782850.021.0767429
AGAGTAC717550.019.07393510-11
GAGTACT494550.018.48108912-13
GTACATG494300.018.386861
CAGAGTA763900.018.21257610-11
TACATGG491950.018.0055752
ACATGGG493950.017.4743
AGTACTT530500.017.1221512-13
GTACTTT560950.016.29236214-15
CATGGGG313350.015.3991294
ACTTTTT654950.014.34517416-17
Produced by FastQC (version 0.11.5)