##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139908_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16685221
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.782149124665477	33.0	31.0	34.0	30.0	34.0
2	31.815172600950266	33.0	31.0	34.0	29.0	34.0
3	31.897701744555857	34.0	31.0	34.0	28.0	34.0
4	35.41041775832637	37.0	35.0	37.0	32.0	37.0
5	35.2466350310853	37.0	35.0	37.0	32.0	37.0
6	35.31493979012924	37.0	35.0	37.0	32.0	37.0
7	35.21710272821679	37.0	35.0	37.0	32.0	37.0
8	35.224149623190485	37.0	35.0	37.0	32.0	37.0
9	36.800273367670705	39.0	37.0	39.0	32.0	39.0
10-11	36.76264674588368	39.0	37.0	39.0	32.0	39.0
12-13	36.735779166485116	39.0	37.0	39.0	32.0	39.0
14-15	37.97060074901016	40.0	38.0	41.0	32.0	41.0
16-17	37.99900618637295	40.0	38.0	41.0	32.0	41.0
18-19	38.0366322088272	40.0	38.0	41.0	32.0	41.0
20-21	38.07988849771647	40.0	38.0	41.0	32.0	41.0
22-23	38.006093068239025	40.0	38.0	41.0	32.0	41.0
24-25	38.019670539422606	40.0	38.0	41.0	32.0	41.0
26-27	37.953336722765584	40.0	37.0	41.0	32.0	41.0
28-29	37.89194175954049	40.0	37.0	41.0	32.0	41.0
30-31	37.83336914318447	40.0	37.0	41.0	32.0	41.0
32-33	37.72782414669116	40.0	37.0	41.0	32.0	41.0
34-35	37.64318547638669	40.0	37.0	41.0	31.0	41.0
36-37	37.59238423019697	40.0	37.0	41.0	31.0	41.0
38-39	37.57752795505178	40.0	37.0	41.0	31.0	41.0
40-41	37.533915479867005	40.0	36.5	41.0	31.0	41.0
42-43	37.49531060786424	40.0	36.0	41.0	31.0	41.0
44-45	37.43800403540729	40.0	36.0	41.0	31.0	41.0
46-47	37.39700563858358	40.0	36.0	41.0	31.0	41.0
48-49	37.33877335674831	40.0	36.0	41.0	31.0	41.0
50-51	37.26149238608818	40.0	36.0	41.0	31.0	41.0
52-53	37.18647557403685	39.5	36.0	41.0	31.0	41.0
54-55	37.22895209342274	39.0	36.0	41.0	31.0	41.0
56-57	37.306603588544235	39.0	36.0	41.0	31.0	41.0
58-59	37.21503782814177	39.0	35.5	41.0	31.0	41.0
60-61	37.08935513363245	39.0	35.0	41.0	31.0	41.0
62-63	36.905960934804384	39.0	35.0	41.0	31.0	41.0
64-65	36.703303891279695	38.5	35.0	41.0	31.0	41.0
66-67	36.45969313675712	38.0	35.0	40.0	30.0	41.0
68-69	36.21808542108526	37.0	35.0	40.0	30.0	41.0
70-71	35.93975819808278	37.0	34.5	39.5	30.0	41.0
72-73	35.671253603923184	36.5	34.0	39.0	30.0	41.0
74-75	35.384893535507615	36.0	34.0	39.0	30.0	41.0
76-77	35.08014642535674	35.5	34.0	38.5	30.0	40.0
78-79	34.771159659725335	35.0	34.0	37.0	29.5	39.0
80-81	34.495859469846586	35.0	34.0	37.0	29.0	39.0
82-83	34.24637695705347	35.0	34.0	36.5	29.0	39.0
84-85	34.027816606429894	35.0	34.0	36.0	29.0	37.0
86-87	33.81689410529797	35.0	34.0	36.0	29.0	37.0
88-89	33.67694785329766	35.0	33.5	35.0	29.0	37.0
90-91	33.5656143881085	35.0	33.0	35.0	29.0	36.0
92-93	33.4870705564146	35.0	33.0	35.0	29.0	36.0
94-95	33.540076970855395	35.0	33.5	35.0	29.5	36.0
96-97	33.703981855486376	35.0	34.0	35.0	30.0	36.0
98-99	33.778503828126354	35.0	34.0	35.0	30.5	36.0
100	33.110008640763205	35.0	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	1.0
14	6.0
15	16.0
16	45.0
17	230.0
18	1635.0
19	8116.0
20	24457.0
21	52622.0
22	86937.0
23	118119.0
24	147650.0
25	175528.0
26	201738.0
27	226702.0
28	256906.0
29	293984.0
30	339710.0
31	401555.0
32	485523.0
33	602016.0
34	784451.0
35	1065311.0
36	1602830.0
37	2888398.0
38	4234072.0
39	2547615.0
40	139045.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.686017883730756	20.93867381199206	14.164828862620398	24.210479441656783
2	14.56714860168594	22.79281949069367	39.973363562614814	22.66666834500558
3	16.976353553410163	27.872712860724064	31.544584512708784	23.60634907315699
4	11.980887962902282	19.559206529021782	40.010135280305796	28.449770227770138
5	12.247268608317977	38.80964791877861	36.27670881994956	12.666374652953857
6	27.72884386813371	40.22411724623487	19.39890135596286	12.648137529668563
7	24.33112605255444	33.89035080960976	24.661135943677735	17.117387194158066
8	23.858953117984054	36.231046380973225	22.329574154442465	17.58042634660026
9	25.48678809011734	15.990299149512424	21.548504102603903	36.974408657766325
10-11	22.94146381618821	27.12391308080637	30.272033998084108	19.662589104921313
12-13	24.40203630890737	25.089538239886338	29.87757196284765	20.630853488358643
14-15	21.73983074236682	26.15687001935546	27.683937545905163	24.41936169237256
16-17	20.98886782986137	29.137551762839582	28.20957474572465	21.664005661574393
18-19	21.02102143497553	28.23351628107656	30.082764747537837	20.662697536410075
20-21	21.99567357440384	27.35629831486659	29.902272135217117	20.74575597551246
22-23	21.607190423822072	27.32440231491344	29.775109718327847	21.29329754293664
24-25	21.337198750791785	27.495036476718738	29.818455908577995	21.349308863911485
26-27	21.38894474194788	27.632754946441707	29.683829752412393	21.294470559198018
28-29	21.36211048873482	27.586762764170757	29.756273537334316	21.294853209760102
30-31	21.619034538058994	27.43028259115362	29.621961213509955	21.32872165727743
32-33	21.462254570654707	27.61203812204044	29.55317867256034	21.372528634744516
34-35	21.611658524185405	27.712290950683826	29.45801077729203	21.218039747838745
36-37	21.606720054766367	27.614105047432748	29.36217234612758	21.417002551673306
38-39	21.485690646499354	27.678522167357034	29.424540945950046	21.41124624019357
40-41	21.73026252086364	27.612808186954357	29.244498131112312	21.41243116106969
42-43	21.651071075412133	27.7188995888954	29.05066236826446	21.579366967428
44-45	21.758706617514594	27.884718417950122	28.725849324202258	21.63072564033303
46-47	21.845999475080223	27.906258177857257	28.366502741036044	21.88123960602648
48-49	21.856983602092566	27.99592414696009	28.304496525292244	21.842595725655105
50-51	21.8401713954231	28.112046107536127	28.124778084998823	21.923004412041955
52-53	21.97424516922336	28.060346142385228	28.066324928269392	21.899083760122018
54-55	21.953903749846955	27.99924512184232	28.136114108939093	21.91073701937163
56-57	21.975765010450402	28.065694627368842	28.05381121702165	21.90472914515911
58-59	21.934101404225476	28.102106763953422	28.09744727683895	21.86634455498215
60-61	22.007407744846134	28.10034709866623	28.052665188291037	21.839579968196603
62-63	21.947014558571794	28.157685413489634	28.013702525201563	21.88159750273701
64-65	21.931615341249092	28.085740387111358	28.031214141227416	21.951430130412135
66-67	21.996756701577205	28.14812186145053	27.90149918213404	21.953622254838226
68-69	21.99111516190542	28.07155882859547	27.95608430496326	21.98124170453585
70-71	22.00488310754852	27.85689988929059	28.03472182662159	22.103495176539298
72-73	22.001452485573793	27.887697746105854	28.006448207069845	22.104401561250512
74-75	21.90539826754139	27.983026819462474	27.990408523553466	22.121166389442667
76-77	21.987159569172952	27.990162222817812	27.9281984372653	22.094479770743934
78-79	21.968559553741592	28.023414363106404	27.89561828685106	22.11240779630095
80-81	21.975983857440188	27.998265861905825	27.895603261822583	22.130147018831405
82-83	22.001042328036892	27.964430888625547	27.953519884866846	22.08100689847071
84-85	22.059019148479635	27.976228714818202	27.913238017284414	22.051514119417746
86-87	21.950026962413798	27.983270192797693	27.954485126827606	22.112217717960895
88-89	22.002654943696815	27.992148239054536	27.88675521045889	22.11844160678975
90-91	22.017159200758023	27.994397776976648	27.97814303534569	22.010299986919637
92-93	22.06571936674421	27.96708161205349	27.917655756537414	22.049543264664884
94-95	21.844138432111954	28.073245457795803	27.957201409215887	22.12541470087635
96-97	21.962583321663573	28.188107151646808	28.02665874963422	21.822650777055397
98-99	22.996226583096664	30.1021612129592	28.22134199554005	18.68027020840409
100	21.99347568474733	31.13234449369106	21.99794060379253	24.87623921776908
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4991.0
1	5064.5
2	7501.0
3	9897.0
4	12213.0
5	20128.0
6	27030.0
7	31861.5
8	34840.5
9	32240.5
10	25845.5
11	19669.5
12	20131.0
13	28445.0
14	50216.5
15	81105.0
16	105779.5
17	118533.5
18	119838.0
19	112576.0
20	101609.0
21	92095.5
22	88310.5
23	95064.0
24	112807.5
25	140104.5
26	176220.0
27	214308.0
28	249031.5
29	285254.5
30	322133.0
31	357573.5
32	396760.0
33	436534.5
34	475989.0
35	511994.5
36	547711.5
37	582095.5
38	607187.0
39	629381.0
40	651724.0
41	669959.0
42	682050.5
43	694251.5
44	702913.0
45	708205.5
46	712790.0
47	713638.0
48	707929.5
49	691435.5
50	669492.5
51	640144.5
52	601650.5
53	560480.0
54	517689.5
55	472482.5
56	429006.0
57	385511.5
58	340734.0
59	295907.0
60	248270.5
61	199808.5
62	156684.5
63	121428.5
64	92107.0
65	69234.5
66	52122.5
67	40048.0
68	31070.0
69	23289.0
70	17495.0
71	13314.5
72	10021.0
73	7716.0
74	5767.5
75	4199.0
76	3058.5
77	2142.5
78	1538.0
79	1110.5
80	798.5
81	598.5
82	459.5
83	320.0
84	229.5
85	179.0
86	145.5
87	113.5
88	79.5
89	57.5
90	45.5
91	37.0
92	27.0
93	13.0
94	3.0
95	2.0
96	2.0
97	1.5
98	2.5
99	4.5
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.012340262079837001
3	0.005370021769564814
4	0.1356469896323219
5	0.061161910891081396
6	0.002828850753610036
7	0.0397357637636325
8	1.0188657375290384E-4
9	5.154261966323371E-4
10-11	2.726964179857132E-4
12-13	1.88789827836263E-4
14-15	3.595996720690724E-5
16-17	6.562694015260571E-4
18-19	2.2474979504317021E-4
20-21	0.004408527375515482
22-23	0.049927582488442354
24-25	7.454171423459996E-4
26-27	6.32014457601581E-5
28-29	0.0019203937404087712
30-31	0.0032402032816488666
32-33	0.00336419228958676
34-35	0.018219066173537277
36-37	0.007694342253961384
38-39	0.003660560736227271
40-41	0.01182010024986629
42-43	0.0030680143366210288
44-45	0.009347065236754546
46-47	0.0026158853206121263
48-49	6.925290676131663E-4
50-51	0.037384568529517326
52-53	6.258780203926211E-5
54-55	7.30569564319195E-5
56-57	0.006748437410202244
58-59	3.851917947660274E-4
60-61	3.887097149264326E-4
62-63	5.194806267096847E-4
64-65	0.004192548917380097
66-67	2.417176667589164E-4
68-69	6.544527621656401E-4
70-71	4.2885671018156574E-5
72-73	9.638860419553962E-4
74-75	7.290571410199189E-6
76-77	1.4726106928324062E-5
78-79	0.0
80-81	5.416331186384909E-4
82-83	0.0
84-85	3.077407176744342E-5
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	3.6918804186805704E-5
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	8766.0
20-21	17545.0
22-23	19203.0
24-25	20316.0
26-27	25801.0
28-29	37777.0
30-31	57069.0
32-33	67694.0
34-35	83443.0
36-37	103353.0
38-39	125659.0
40-41	128647.0
42-43	149602.0
44-45	179573.0
46-47	171685.0
48-49	149580.0
50-51	130539.0
52-53	121268.0
54-55	125692.0
56-57	131235.0
58-59	133281.0
60-61	132209.0
62-63	126620.0
64-65	131563.0
66-67	138230.0
68-69	141991.0
70-71	142896.0
72-73	134721.0
74-75	133774.0
76-77	138717.0
78-79	146016.0
80-81	151306.0
82-83	145516.0
84-85	144945.0
86-87	149712.0
88-89	159339.0
90-91	169678.0
92-93	175312.0
94-95	198740.0
96-97	529412.0
98-99	1025083.0
100-101	1.0481713E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.57080804453702
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.74752044347755	75.90020323898501
2	7.043900068635585	11.91416641178962
3	1.7847626923480508	4.528164691788541
4	0.6589087421960157	2.2289777902050627
5	0.2828908933012664	1.19621557174645
6	0.15996168281980436	0.8116853263340874
7	0.09120945223937543	0.5399559954028511
8	0.060562022913674796	0.40974233716969943
9	0.03844588081726943	0.2926259286030396
>10	0.12674158306384573	1.691423361327181
>50	0.003471041744310962	0.1999932013835973
>100	0.0015485717696027623	0.22275178121057823
>500	3.850062805260847E-5	0.020084630921025883
>1k	3.842404561484408E-5	0.04400973313323264
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	6.592660654599661E-5	0.0	5.993327867817873E-6	5.993327867817873E-6
5	5.993327867817873E-6	6.592660654599661E-5	0.0	5.993327867817873E-6	5.993327867817873E-6
6	5.993327867817873E-6	6.592660654599661E-5	0.0	5.993327867817873E-6	2.3973311471271493E-5
7	5.993327867817873E-6	7.191993441381447E-5	0.0	1.7979983603453618E-5	2.3973311471271493E-5
8	1.1986655735635746E-5	8.390659014945023E-5	0.0	1.7979983603453618E-5	2.3973311471271493E-5
9	1.1986655735635746E-5	1.8579316390235405E-4	0.0	2.3973311471271493E-5	2.9966639339089365E-5
10-11	2.696997540518043E-5	1.8579316390235405E-4	0.0	2.696997540518043E-5	2.9966639339089365E-5
12-13	3.29633032729983E-5	1.8579316390235405E-4	0.0	4.195329507472511E-5	2.9966639339089365E-5
14-15	3.5959967206907236E-5	2.6070976225007743E-4	0.0	5.993327867817873E-5	4.494995900863405E-5
16-17	3.5959967206907236E-5	2.7569308191962216E-4	0.0	8.09099262155413E-5	5.993327867817873E-5
18-19	3.5959967206907236E-5	2.8168640978744005E-4	0.0	9.888990981899492E-5	6.592660654599661E-5
20-21	3.5959967206907236E-5	3.0865638519262046E-4	0.0	1.1986655735635746E-4	6.592660654599661E-5
22-23	4.1953295074725114E-5	3.5360634420125454E-4	0.0	1.468365327615379E-4	7.791326228163235E-5
24-25	4.7946622942542986E-5	3.835729835403439E-4	0.0	2.0077648357189873E-4	8.390659014945023E-5
26-27	5.393995081036086E-5	4.045496310777064E-4	0.0	2.637064261839864E-4	8.98999180172681E-5
28-29	5.393995081036086E-5	4.0754629501161535E-4	0.0	4.914528851610656E-4	8.98999180172681E-5
30-31	5.69366147442698E-5	4.584895818880673E-4	0.0	0.0012885654915808426	9.589324588508597E-5
32-33	6.59266065459966E-5	4.614862458219762E-4	0.0	0.0026310709339720465	1.1087656555463065E-4
34-35	7.191993441381447E-5	5.334061802357906E-4	0.0	0.0043062060730271415	1.3185321309199321E-4
36-37	8.390659014945023E-5	6.053261146496051E-4	0.0	0.0068293971053784664	1.3484987702590213E-4
38-39	8.390659014945023E-5	6.11319442517423E-4	0.0	0.011036713268586613	1.3784654095981108E-4
40-41	8.390659014945023E-5	6.952260326668733E-4	0.0	0.015522719177648291	1.468365327615379E-4
42-43	8.989991801726811E-5	7.042160244686E-4	0.0	0.01955323216875581	1.4983319669544684E-4
44-45	9.589324588508597E-5	7.611526392128699E-4	0.0	0.024275974528596297	1.5282986062935576E-4
46-47	9.589324588508597E-5	8.150925900232307E-4	0.0	0.029675962937500196	1.7080984423280938E-4
48-49	9.589324588508597E-5	8.150925900232307E-4	0.0	0.035057971362800644	1.797998360345362E-4
50-51	9.589324588508597E-5	9.199758277100435E-4	0.0	0.04039802649302637	1.827964999684451E-4
52-53	9.589324588508597E-5	9.349591473795882E-4	0.0	0.045681145008507826	1.9478315570408086E-4
54-55	9.589324588508597E-5	9.529391309830418E-4	0.0	0.05088335359777374	2.0676981143971662E-4
56-57	9.589324588508597E-5	9.94892426057767E-4	0.0	0.056238392047669006	2.127631393075345E-4
58-59	9.88899098189949E-5	9.94892426057767E-4	0.0	0.06160841381723382	2.1575980324144343E-4
60-61	1.0488323768681278E-4	0.0010068790817934028	0.0	0.06715523875889927	2.1575980324144343E-4
62-63	1.0787990162072172E-4	0.0010188657375290384	0.0	0.07251627053666235	2.3973311471271492E-4
64-65	1.0787990162072172E-4	0.0011207523112819423	0.0	0.07762258588004319	2.4272977864662386E-4
66-67	1.138732294885396E-4	0.001132738967017578	0.0	0.08267196460867973	2.5771309831616854E-4
68-69	1.138732294885396E-4	0.001186678917827939	0.0	0.08713399720627014	2.6370642618398643E-4
70-71	1.138732294885396E-4	0.0012046589014313926	0.0	0.09081090385317642	2.667030901178953E-4
72-73	1.138732294885396E-4	0.0012046589014313926	0.0	0.09375362783627499	2.7569308191962216E-4
74-75	1.138732294885396E-4	0.0012496088604400267	0.0	0.09618392228667513	2.7569308191962216E-4
76-77	1.138732294885396E-4	0.0013544920981268392	0.0	0.0986591666960839	2.7569308191962216E-4
78-79	1.1686989342244853E-4	0.0013844587374659287	0.0	0.10032531184333729	2.7569308191962216E-4
80-81	1.1986655735635746E-4	0.0013904520653337466	0.0	0.10090966131044954	2.8767973765525794E-4
82-83	1.1986655735635746E-4	0.0013964453932015645	0.0	0.10124828433498123	3.29633032729983E-4
84-85	1.3784654095981108E-4	0.0013964453932015645	0.0	0.10138912753987496	3.29633032729983E-4
86-87	1.3784654095981108E-4	0.0014383986882762895	0.0	0.10146104747428877	3.3262969666389197E-4
88	1.3784654095981108E-4	0.0015522719177648292	0.0	0.10147902745789222	3.4161968846561875E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	23750	0.0	49.681126	1
GTATCAA	44920	0.0	37.943718	1
TCAACGC	56665	0.0	29.58216	4
ATCAACG	56815	0.0	29.519716	3
CAACGCA	57760	0.0	29.015755	5
TATCAAC	58695	0.0	28.709944	2
AACGCAG	59000	0.0	28.424194	6
ACGCAGA	67010	0.0	24.982323	7
CGCAGAG	67790	0.0	24.685623	8
GCAGAGT	78285	0.0	21.076742	9
AGAGTAC	71755	0.0	19.073935	10-11
GAGTACT	49455	0.0	18.481089	12-13
GTACATG	49430	0.0	18.38686	1
CAGAGTA	76390	0.0	18.212576	10-11
TACATGG	49195	0.0	18.005575	2
ACATGGG	49395	0.0	17.474	3
AGTACTT	53050	0.0	17.12215	12-13
GTACTTT	56095	0.0	16.292362	14-15
CATGGGG	31335	0.0	15.399129	4
ACTTTTT	65495	0.0	14.345174	16-17
>>END_MODULE