FastQCFastQC Report
Fri 27 May 2016
SRR2139907_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139907_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51564777
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA772700.050.468261
GTATCAA1413750.037.4623261
TCAACGC1754350.029.6627854
ATCAACG1760050.029.5694963
CAACGCA1783800.029.1666035
TATCAAC1822300.028.7359222
AACGCAG1838750.028.3539896
ACGCAGA2085050.024.9452197
CGCAGAG2117600.024.5618258
GCAGAGT2432200.021.1103959
GAGTACT1449750.019.35030612-13
GTACATG1628350.019.245771
AGAGTAC2218600.019.0513510-11
TACATGG1617100.018.8639532
CAGAGTA2375250.018.6698810-11
ACATGGG1643900.018.1547163
AGTACTT1575750.017.394212-13
CTTATAC305850.017.1212921
GTACTTT1647700.017.01754214-15
CATGGGG1053800.016.1005154
Produced by FastQC (version 0.11.5)