##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139907_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	51564777
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.798265878275785	33.0	31.0	34.0	30.0	34.0
2	31.836209162700346	33.0	31.0	34.0	29.0	34.0
3	31.914636244814943	34.0	31.0	34.0	29.0	34.0
4	35.420727796418085	37.0	35.0	37.0	33.0	37.0
5	35.25935486931321	37.0	35.0	37.0	32.0	37.0
6	35.322473924399986	37.0	35.0	37.0	32.0	37.0
7	35.22739727934827	37.0	35.0	37.0	32.0	37.0
8	35.23481726683313	37.0	35.0	37.0	32.0	37.0
9	36.81294851716318	39.0	37.0	39.0	32.0	39.0
10-11	36.77461975642792	39.0	37.0	39.0	32.0	39.0
12-13	36.74401783023322	39.0	37.0	39.0	32.0	39.0
14-15	37.988279596360904	40.0	38.0	41.0	32.0	41.0
16-17	38.01439863688347	40.0	38.0	41.0	32.0	41.0
18-19	38.05241004920859	40.0	38.0	41.0	32.0	41.0
20-21	38.09673611756059	40.0	38.0	41.0	32.0	41.0
22-23	38.02356713303239	40.0	38.0	41.0	32.0	41.0
24-25	38.037407572711274	40.0	38.0	41.0	32.0	41.0
26-27	37.970990601533146	40.0	37.5	41.0	32.0	41.0
28-29	37.910366177353474	40.0	37.0	41.0	32.0	41.0
30-31	37.85336441505073	40.0	37.0	41.0	32.0	41.0
32-33	37.749326957795006	40.0	37.0	41.0	32.0	41.0
34-35	37.6642743966033	40.0	37.0	41.0	31.0	41.0
36-37	37.61421462055564	40.0	37.0	41.0	31.0	41.0
38-39	37.59368019814349	40.0	37.0	41.0	31.0	41.0
40-41	37.55222702044048	40.0	37.0	41.0	31.0	41.0
42-43	37.51568232047116	40.0	36.0	41.0	31.0	41.0
44-45	37.45560521300574	40.0	36.0	41.0	31.0	41.0
46-47	37.41370996667581	40.0	36.0	41.0	31.0	41.0
48-49	37.35385123123352	40.0	36.0	41.0	31.0	41.0
50-51	37.27081137437555	40.0	36.0	41.0	31.0	41.0
52-53	37.191667533501175	39.5	36.0	41.0	31.0	41.0
54-55	37.23591255152539	39.0	36.0	41.0	31.0	41.0
56-57	37.31511110983617	39.0	36.0	41.0	31.0	41.0
58-59	37.22123881071718	39.0	35.5	41.0	31.0	41.0
60-61	37.09199790801031	39.0	35.0	41.0	31.0	41.0
62-63	36.90805965231772	39.0	35.0	41.0	31.0	41.0
64-65	36.7067545840129	38.5	35.0	41.0	31.0	41.0
66-67	36.463183257404225	38.0	35.0	40.0	30.0	41.0
68-69	36.22196397080282	37.0	35.0	40.0	30.0	41.0
70-71	35.94220935254556	37.0	34.5	39.5	30.0	41.0
72-73	35.67442453246005	36.5	34.0	39.0	30.0	41.0
74-75	35.38675056922152	36.0	34.0	39.0	30.0	41.0
76-77	35.083452735815996	35.5	34.0	38.5	30.0	40.0
78-79	34.77381785261586	35.0	34.0	37.0	30.0	39.0
80-81	34.500502187715725	35.0	34.0	37.0	29.0	39.0
82-83	34.25154741276597	35.0	34.0	36.5	29.0	39.0
84-85	34.0306848397225	35.0	34.0	36.0	29.0	37.0
86-87	33.81969628454459	35.0	34.0	36.0	29.0	37.0
88-89	33.67982024632561	35.0	33.5	35.0	29.0	37.0
90-91	33.56881629797968	35.0	33.0	35.0	29.0	36.0
92-93	33.49010347152452	35.0	33.0	35.0	29.0	36.0
94-95	33.54298641696539	35.0	33.5	35.0	29.5	36.0
96-97	33.705292979919484	35.0	34.0	35.0	30.0	36.0
98-99	33.77970865482267	35.0	34.0	35.0	30.5	36.0
100	33.11365931127713	35.0	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	2.0
13	10.0
14	31.0
15	58.0
16	133.0
17	600.0
18	4999.0
19	24316.0
20	74259.0
21	162117.0
22	266000.0
23	364863.0
24	456431.0
25	542738.0
26	624362.0
27	703651.0
28	795698.0
29	910310.0
30	1054180.0
31	1239473.0
32	1495936.0
33	1857507.0
34	2420563.0
35	3286395.0
36	4958640.0
37	8978102.0
38	1.3209704E7
39	7745763.0
40	387934.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.45341260760228	20.981349342400918	14.35907305484905	24.206164995147752
2	14.438747005692882	22.521394488622626	40.44426639342235	22.59559211226214
3	16.96410228403162	27.903427846232653	31.53525558644155	23.59721428329418
4	11.985870754101894	19.680998679024256	39.79649108838983	28.536639478484023
5	12.280244236008391	38.802189162012574	36.20292311817539	12.714643483803648
6	27.793813709228083	40.04179905231175	19.47239872025432	12.691988518205852
7	24.561315659665652	33.830387695107405	24.477033819344644	17.131262825882295
8	23.96605487473197	36.456994179725825	22.070544970902894	17.506405974639307
9	25.64801011019403	16.088818239199	21.349550560754007	36.91362108985296
10-11	23.226259787658538	27.05368666871639	30.02077662381395	19.69927691981112
12-13	24.604039143077358	24.95294045447678	29.694577346185568	20.748443056260292
14-15	21.858095650669345	26.007550466343666	27.463512776418902	24.670841106568087
16-17	21.115262175194534	29.048946490189042	27.995686366893764	21.840104967722656
18-19	21.170887786962965	28.14108199048232	29.8576769659411	20.830353256613616
20-21	22.17760543647571	27.238722215488455	29.65832014911374	20.925352198922095
22-23	21.79907226699415	27.287201704665154	29.486738916033232	21.426987112307465
24-25	21.510254114109394	27.463348306527564	29.581220855696007	21.44517672366704
26-27	21.56332839087328	27.58390906291586	29.445579187240114	21.407183358970745
28-29	21.483771886612324	27.555932211225997	29.515367850797926	21.444928051363753
30-31	21.8375944692873	27.409738038942066	29.39290521401436	21.35976227775628
32-33	21.537165581135806	27.66528127879132	29.299936151245593	21.497616988827282
34-35	21.71853754977627	27.655873232277195	29.294260196044284	21.33132902190225
36-37	21.711073081166766	27.60672717403459	29.19749274009788	21.484707004700763
38-39	21.588660715924902	27.591128222995952	29.269166713759347	21.551044347319795
40-41	21.83436561621461	27.503505273697176	29.112245955221056	21.54988315486716
42-43	21.789903572297963	27.60670226449326	28.90765773145201	21.695736431756767
44-45	21.863447127087866	27.76645671740528	28.65061239885408	21.719483756652775
46-47	21.97349671224636	27.777011933104887	28.23708202986708	22.01240932478168
48-49	21.97709818887975	27.868308241033358	28.1887247214039	21.965868848682984
50-51	21.94099995236643	28.0093884914583	28.036034275186694	22.013577280988578
52-53	22.077194840307058	27.92523107739717	28.023958833394286	21.973615248901485
54-55	22.04738287378077	27.87236885059725	28.050892466628063	22.029355808993916
56-57	22.06332031715337	28.00240221324498	27.939592277201257	21.994685192400397
58-59	22.018865813692177	28.079697970547073	27.992386610594323	21.909049605166427
60-61	22.119760071196357	28.0952152316445	27.891541024484575	21.89348367267457
62-63	22.011052348529088	28.183259708233336	27.87161757598067	21.934070367256904
64-65	22.000987601983123	28.121948709255314	27.87137295590299	22.00569073285857
66-67	22.081005678833325	28.121123444298433	27.753400208589184	22.04447066827905
68-69	22.04397571536768	28.054152629864838	27.839499380492576	22.062372274274907
70-71	22.076340968760224	27.80463027669255	27.93075675201184	22.18827200253539
72-73	22.082958711203542	27.85408107189496	27.891004494729277	22.17195572217222
74-75	21.964374737212047	27.98045574712353	27.86382999481142	22.191339520853003
76-77	22.104178767331273	27.943925416328703	27.79618391696914	22.155711899370882
78-79	22.06631652987754	27.973635912545763	27.77279572459545	22.187251832981246
80-81	22.07284221943631	27.953703045002687	27.783883979305084	22.189570756255918
82-83	22.110288616770504	27.93029526570139	27.856735472311385	22.102680645216726
84-85	22.15226980866307	27.921127242590483	27.80757367408613	22.119029274660317
86-87	22.033152927551487	27.935160078631245	27.88888529121388	22.142801702603386
88-89	22.08631937173178	27.94230023305902	27.82697827039393	22.14440212481527
90-91	22.100602224158575	27.960229977859306	27.88626135819411	22.052906439788007
92-93	22.098911857208037	27.94541286379563	27.836983519017	22.118691759979328
94-95	21.950893430329423	28.024262751491193	27.888098537727924	22.136745280451457
96-97	22.029716434052077	28.155335955413346	27.93826069743063	21.876686913103946
98-99	23.06552946708204	30.093422274484382	28.151228980080656	18.68981927835292
100	22.093591300342748	31.15744501402688	21.872830917758808	24.87613276787156
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	11848.0
1	12296.0
2	19189.5
3	25835.5
4	32837.0
5	55868.0
6	75480.5
7	86515.5
8	90983.5
9	83317.5
10	68687.5
11	53886.0
12	56673.5
13	81685.0
14	145071.0
15	236454.0
16	312204.0
17	355710.5
18	365499.0
19	344592.0
20	307074.0
21	271231.5
22	252439.0
23	266072.5
24	313232.0
25	389330.0
26	491503.5
27	603241.5
28	714287.5
29	835035.5
30	953883.5
31	1071153.5
32	1200788.5
33	1321722.5
34	1440110.5
35	1554497.0
36	1666274.0
37	1771644.5
38	1847408.0
39	1909362.0
40	1979522.0
41	2037216.0
42	2073028.5
43	2114560.5
44	2164164.0
45	2207532.5
46	2216113.5
47	2203113.5
48	2180806.0
49	2131561.0
50	2070936.5
51	1982242.5
52	1865000.5
53	1739695.5
54	1605280.5
55	1470199.5
56	1339859.0
57	1203390.5
58	1064997.0
59	929051.5
60	779264.5
61	629886.5
62	497787.0
63	386988.5
64	296435.5
65	224610.5
66	169990.5
67	130888.0
68	102135.0
69	77763.5
70	59048.5
71	45204.5
72	33657.5
73	24762.0
74	18018.5
75	13103.0
76	9498.5
77	6687.5
78	4912.5
79	3673.5
80	2693.0
81	2030.5
82	1600.0
83	1144.5
84	805.5
85	600.5
86	464.5
87	361.0
88	238.0
89	142.5
90	97.5
91	80.0
92	61.0
93	35.5
94	15.5
95	9.5
96	8.5
97	6.0
98	4.5
99	9.0
100	30.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.012648168729596175
3	0.005333097823733437
4	0.13333132420993501
5	0.062055926276962284
6	0.0027286067774519806
7	0.04082631832190412
8	4.654339918894636E-5
9	3.6458996031341317E-4
10-11	1.8423428845624603E-4
12-13	1.0181368572582017E-4
14-15	2.9089624493091474E-5
16-17	6.874847921867286E-4
18-19	2.1138460464979807E-4
20-21	0.004239667295675183
22-23	0.05084012641030472
24-25	6.710568502538487E-4
26-27	8.472058090974206E-5
28-29	0.001854177421741033
30-31	0.003138529715178917
32-33	0.003170850839154098
34-35	0.018456111820180716
36-37	0.007789314918913767
38-39	0.0034903647852554563
40-41	0.011893252459751796
42-43	0.00301345184679902
44-45	0.009487095952161847
46-47	0.002729730159674359
48-49	7.407683926983491E-4
50-51	0.03812700592125086
52-53	4.9767819884118286E-5
54-55	4.799535712113353E-5
56-57	0.006718935947519565
58-59	3.1738061725373974E-4
60-61	4.1394583292689644E-4
62-63	5.450704194643921E-4
64-65	0.004363276088875195
66-67	2.3283487044273033E-4
68-69	6.437455383210821E-4
70-71	3.990043814671129E-5
72-73	9.617599194146426E-4
74-75	4.64135894534215E-6
76-77	1.98983139971272E-5
78-79	3.544030012925314E-6
80-81	6.144008985189622E-4
82-83	0.0
84-85	2.9209599554767813E-5
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	3.22370567701275E-5
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	26725.0
20-21	53216.0
22-23	58853.0
24-25	62800.0
26-27	76449.0
28-29	107875.0
30-31	167678.0
32-33	193901.0
34-35	237933.0
36-37	294746.0
38-39	366608.0
40-41	372100.0
42-43	431386.0
44-45	518361.0
46-47	495776.0
48-49	425766.0
50-51	370352.0
52-53	339580.0
54-55	348321.0
56-57	365178.0
58-59	375666.0
60-61	378335.0
62-63	359973.0
64-65	373239.0
66-67	392646.0
68-69	406135.0
70-71	408501.0
72-73	374444.0
74-75	370204.0
76-77	386352.0
78-79	408967.0
80-81	423788.0
82-83	410127.0
84-85	405130.0
86-87	419928.0
88-89	452229.0
90-91	487691.0
92-93	509220.0
94-95	583608.0
96-97	1647097.0
98-99	3242093.0
100-101	3.34358E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.57575474418151
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.68207395939896	68.11109384150735
2	8.5389377840293	13.419069621874218
3	2.3567749599842096	5.555561137288423
4	0.9789607600337671	3.076903223383632
5	0.4822193260941932	1.8945373750040906
6	0.28817947901375485	1.358635203917448
7	0.1830416908395976	1.006784730516076
8	0.1166276935796156	0.7331287237673142
9	0.07825986904011234	0.5534395448406867
>10	0.28595683447275205	3.509215842698416
>50	0.006490907359835466	0.347153102705957
>100	0.0023581277567205697	0.32075412957977895
>500	7.607848117564605E-5	0.04109345545088838
>1k	4.252991598065927E-5	0.07263006746577298
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.7150316193552043E-5	0.0	0.0	0.0
3	0.0	2.7150316193552043E-5	0.0	3.878616599078863E-6	0.0
4	0.0	8.145094858065614E-5	0.0	7.757233198157726E-6	1.9393082995394316E-6
5	7.757233198157726E-6	8.145094858065614E-5	0.0	1.163584979723659E-5	1.9393082995394316E-6
6	3.1028932792630906E-5	8.145094858065614E-5	0.0	1.163584979723659E-5	3.878616599078863E-6
7	3.1028932792630906E-5	8.339025688019557E-5	0.0	2.133239129493375E-5	3.878616599078863E-6
8	3.296824109217034E-5	1.0278333987558988E-4	0.0	2.133239129493375E-5	3.878616599078863E-6
9	3.296824109217034E-5	1.9780944655302203E-4	0.0	2.327169959447318E-5	5.817924898618295E-6
10-11	4.5573745039176646E-5	1.9780944655302203E-4	0.0	2.5211007894012612E-5	5.817924898618295E-6
12-13	6.399717388480124E-5	1.9974875485256148E-4	0.0	2.5211007894012612E-5	7.757233198157726E-6
14-15	7.466336953226812E-5	2.744121243848296E-4	0.0	2.9089624493091474E-5	1.7453774695854887E-5
16-17	1.0763161062443845E-4	2.821693575829873E-4	0.0	3.1028932792630906E-5	2.5211007894012612E-5
18-19	1.1829780627190534E-4	3.015624405783816E-4	0.0	3.781651184101892E-5	2.5211007894012612E-5
20-21	1.221764228709842E-4	3.335610275207822E-4	0.0	5.0422015788025224E-5	2.5211007894012612E-5
22-23	1.3187296436868137E-4	3.8786165990788634E-4	0.0	6.593648218434069E-5	3.393789524194005E-5
24-25	1.5417500981338483E-4	4.33435404947063E-4	0.0	9.599576082720186E-5	3.6846857691249206E-5
26-27	1.6290189716131227E-4	4.5379814209222703E-4	0.0	1.5708397226269397E-4	3.6846857691249206E-5
28-29	1.6968947620970028E-4	4.586464128410756E-4	0.0	4.033761263042018E-4	3.6846857691249206E-5
30-31	1.7453774695854886E-4	5.313704740738043E-4	0.0	0.0013507282306292141	3.6846857691249206E-5
32-33	1.8520394260601572E-4	5.410670155715014E-4	0.0	0.0031242256705580245	3.878616599078864E-5
34-35	2.133239129493375E-4	6.506379344954794E-4	0.0	0.005313704740738043	4.072547429032807E-5
36-37	2.3659561254381068E-4	7.514819660715298E-4	0.0	0.008471868306538007	4.5573745039176646E-5
38-39	2.433831915921987E-4	7.854198613134699E-4	0.0	0.01361879253351566	4.945236163825551E-5
40-41	2.6083696628805355E-4	8.600832308457379E-4	0.0	0.019575377975551023	5.2361324087564655E-5
42-43	2.6568523703690214E-4	8.833549304402112E-4	0.0	0.024856114475196894	5.52702865368738E-5
44-45	2.802300492834479E-4	9.871079244655708E-4	0.0	0.030829184037778345	5.9148903135952664E-5
46-47	2.9477486152999365E-4	0.0010666195647466876	0.0	0.03746355773050274	6.593648218434067E-5
48-49	3.0253209472815137E-4	0.001072437489645306	0.0	0.04389527370592527	7.175440708295898E-5
50-51	3.064107113272302E-4	0.0012140069955116843	0.0	0.050425894404624305	7.175440708295898E-5
52-53	3.180465611244668E-4	0.001221764228709842	0.0	0.05674997876942239	8.145094858065614E-5
54-55	3.2289483187331535E-4	0.001232430424357309	0.0	0.06235651906339089	9.308679837789272E-5
56-57	3.238644860230851E-4	0.0012605503947006307	0.0	0.06649694228290758	9.502610667743215E-5
58-59	3.238644860230851E-4	0.0012789738235462552	0.0	0.07070427163875836	9.599576082720186E-5
60-61	3.325913733710125E-4	0.0013061241397398073	0.0	0.07503377741748016	1.0181368572582017E-4
62-63	3.4325756901847944E-4	0.0013352137642328988	0.0	0.07919553302829177	1.0472264817512931E-4
64-65	3.451968773180189E-4	0.0014990853155439807	0.0	0.08309742132696511	1.0569230232489903E-4
66-67	3.5295411051617656E-4	0.0015213873609886842	0.0	0.08708948746156703	1.0763161062443845E-4
68-69	3.665292686129526E-4	0.0015853845348734854	0.0	0.09095258959424958	1.0860126477420817E-4
70-71	3.762258101106498E-4	0.0016338672423619714	0.0	0.09435995427654036	1.095709189239779E-4
72-73	3.965885472558138E-4	0.0016551996336569052	0.0	0.09719716231876654	1.163584979723659E-4
74-75	4.043457804539715E-4	0.001713378882643088	0.0	0.09940312550949265	1.163584979723659E-4
76-77	4.1695128440097785E-4	0.00180937464347029	0.0	0.10144521714890767	1.1732815212213562E-4
78-79	4.2276920929959613E-4	0.0018539787343596968	0.0	0.10284248877872584	1.1926746042167506E-4
80-81	4.314960966475236E-4	0.0018549483885094665	0.0	0.10332052827456231	1.2411573117052365E-4
82-83	4.344050590968327E-4	0.0018636752758573938	0.0	0.10360075832384576	1.3381227266822076E-4
84-85	4.411926381452207E-4	0.0018820987047030186	0.0	0.10367348238507848	1.357515809677602E-4
86-87	4.4701056304383906E-4	0.0019315510663412742	0.0	0.10371226855106927	1.3672123511752993E-4
88	4.4991952549314814E-4	0.002090574346903507	0.0	0.10371420785936881	1.3769088926729965E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	77270	0.0	50.46826	1
GTATCAA	141375	0.0	37.462326	1
TCAACGC	175435	0.0	29.662785	4
ATCAACG	176005	0.0	29.569496	3
CAACGCA	178380	0.0	29.166603	5
TATCAAC	182230	0.0	28.735922	2
AACGCAG	183875	0.0	28.353989	6
ACGCAGA	208505	0.0	24.945219	7
CGCAGAG	211760	0.0	24.561825	8
GCAGAGT	243220	0.0	21.110395	9
GAGTACT	144975	0.0	19.350306	12-13
GTACATG	162835	0.0	19.24577	1
AGAGTAC	221860	0.0	19.05135	10-11
TACATGG	161710	0.0	18.863953	2
CAGAGTA	237525	0.0	18.66988	10-11
ACATGGG	164390	0.0	18.154716	3
AGTACTT	157575	0.0	17.3942	12-13
CTTATAC	30585	0.0	17.121292	1
GTACTTT	164770	0.0	17.017542	14-15
CATGGGG	105380	0.0	16.100515	4
>>END_MODULE