FastQCFastQC Report
Fri 27 May 2016
SRR2139906_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139906_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18666565
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA294700.048.059581
GTATCAA516600.036.1580621
ATCAACG621100.029.494153
TCAACGC623800.029.365864
CAACGCA634350.028.8571645
TATCAAC650850.028.3023532
AACGCAG656050.027.9247536
ACGCAGA750050.024.3735757
CGCAGAG759050.024.0690048
GCAGAGT889250.020.2737249
GTACATG732700.019.359031
TACATGG721600.019.18672
GAGTACT439950.018.95065712-13
ACATGGG729800.018.6108723
CAGAGTA864950.018.29242510-11
AGAGTAC810250.017.8354510-11
GTACTTT492850.016.9122414-15
CATGGGG443500.016.5694944
AGTACTT479150.015.89910712-13
GTGGTAT126850.015.1767591
Produced by FastQC (version 0.11.5)