##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139906_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18666565
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.824699884526158	33.0	31.0	34.0	30.0	34.0
2	31.86307978998814	33.0	31.0	34.0	29.0	34.0
3	31.945049022141994	34.0	31.0	34.0	29.0	34.0
4	35.453192325422485	37.0	35.0	37.0	33.0	37.0
5	35.29147103390474	37.0	35.0	37.0	32.0	37.0
6	35.35515682719343	37.0	35.0	37.0	32.0	37.0
7	35.263399238156566	37.0	35.0	37.0	32.0	37.0
8	35.267962905869396	37.0	35.0	37.0	32.0	37.0
9	36.85515481825392	39.0	37.0	39.0	32.0	39.0
10-11	36.81627578507347	39.0	37.0	39.0	32.0	39.0
12-13	36.78879981399899	39.0	37.0	39.0	32.0	39.0
14-15	38.03722974741201	40.0	38.0	41.0	32.0	41.0
16-17	38.05893194596864	40.0	38.0	41.0	32.0	41.0
18-19	38.09218900210081	40.0	38.0	41.0	32.0	41.0
20-21	38.135250107414976	40.0	38.0	41.0	32.0	41.0
22-23	38.061618964463044	40.0	38.0	41.0	32.0	41.0
24-25	38.07514852842855	40.0	38.0	41.0	32.0	41.0
26-27	38.010402948159225	40.0	38.0	41.0	32.0	41.0
28-29	37.95180961406673	40.0	37.0	41.0	32.0	41.0
30-31	37.90306543142394	40.0	37.0	41.0	32.0	41.0
32-33	37.808654448915746	40.0	37.0	41.0	32.0	41.0
34-35	37.72913090294941	40.0	37.0	41.0	31.5	41.0
36-37	37.67977239232925	40.0	37.0	41.0	31.0	41.0
38-39	37.66364678046592	40.0	37.0	41.0	31.0	41.0
40-41	37.62232571221962	40.0	37.0	41.0	31.0	41.0
42-43	37.583938191599984	40.0	37.0	41.0	31.0	41.0
44-45	37.52733997356258	40.0	37.0	41.0	31.0	41.0
46-47	37.489005846511674	40.0	36.5	41.0	31.0	41.0
48-49	37.43190389163867	40.0	36.0	41.0	31.0	41.0
50-51	37.35037930558819	40.0	36.0	41.0	31.0	41.0
52-53	37.26905994613252	40.0	36.0	41.0	31.0	41.0
54-55	37.3108778083003	39.5	36.0	41.0	31.0	41.0
56-57	37.38232793979219	40.0	36.0	41.0	31.0	41.0
58-59	37.288833213060684	39.0	36.0	41.0	31.0	41.0
60-61	37.161158589470226	39.0	35.0	41.0	31.0	41.0
62-63	36.97745122338633	39.0	35.0	41.0	31.0	41.0
64-65	36.772857410938784	38.5	35.0	41.0	31.0	41.0
66-67	36.52652558260761	38.0	35.0	40.0	30.5	41.0
68-69	36.28107984017798	37.0	35.0	40.0	30.0	41.0
70-71	35.99639004957778	37.0	35.0	39.5	30.0	41.0
72-73	35.720955421793605	36.5	34.0	39.0	30.0	41.0
74-75	35.425590295791004	36.0	34.0	39.0	30.0	41.0
76-77	35.111919656350494	35.5	34.0	38.5	30.0	40.0
78-79	34.793345992896505	35.0	34.0	37.0	30.0	39.0
80-81	34.51221066382496	35.0	34.0	37.0	30.0	39.0
82-83	34.25587946861166	35.0	34.0	36.5	29.0	38.5
84-85	34.032386168874154	35.0	34.0	36.0	29.0	37.0
86-87	33.819808069629616	35.0	34.0	36.0	29.0	37.0
88-89	33.6825422492326	35.0	34.0	35.0	29.0	36.5
90-91	33.57403194311824	35.0	33.5	35.0	29.0	36.0
92-93	33.498356502439826	35.0	33.0	35.0	29.0	36.0
94-95	33.55341514624957	35.0	33.5	35.0	29.5	36.0
96-97	33.718457222217936	35.0	34.0	35.0	30.0	36.0
98-99	33.79354965802443	35.0	34.0	35.0	31.0	35.5
100	33.13102169199284	35.0	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	7.0
15	17.0
16	35.0
17	221.0
18	1610.0
19	8339.0
20	25818.0
21	55577.0
22	92790.0
23	127467.0
24	160082.0
25	190665.0
26	219474.0
27	247979.0
28	279836.0
29	321932.0
30	372730.0
31	439328.0
32	531868.0
33	663538.0
34	867077.0
35	1176356.0
36	1777780.0
37	3239175.0
38	4900212.0
39	2828307.0
40	138342.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.18287242457303	21.25494433496468	14.526920191261755	24.035263049200537
2	14.370247163949948	22.608713309738494	40.58542183128592	22.435617695025638
3	17.027131613292905	28.062284091098427	31.535063302918864	23.3755209926898
4	12.057874113637347	19.78175570146064	39.82279854700495	28.337571637897067
5	12.344816565736291	38.981113795118816	36.032232536380846	12.641837102764045
6	27.798502575130403	39.832455134443315	19.690700413504842	12.678341876921436
7	24.782932587310647	33.87297248773377	24.427103347187273	16.916991577768307
8	24.199298970581008	36.28073941251808	22.07624451632704	17.443717100573867
9	26.00636659835707	16.0275987715522	21.32633126443798	36.63970336565276
10-11	23.503242074493745	26.988631866340597	29.867210656640587	19.64091540252507
12-13	24.80314571496141	24.88539718578201	29.51730651893063	20.794150580325955
14-15	22.086248205447	25.95545407638522	27.304095964352076	24.65420175381571
16-17	21.27656371537622	28.971224873290492	27.857039405501315	21.89517200583197
18-19	21.350136530485827	28.18483856155995	29.538435296506947	20.926589611447273
20-21	22.585220780515154	27.224671164681208	29.18947088983898	21.00063716496466
22-23	22.18171464425365	27.276243850059274	28.988264716031708	21.55377678965537
24-25	21.76127241583176	27.460191341574824	29.1269130732706	21.651623169322814
26-27	21.76465072082598	27.565923177818014	29.030990554206248	21.638435547149758
28-29	21.69515226480731	27.557531022411286	29.093243799934587	21.654072912846814
30-31	22.00831434163338	27.424520357742928	28.987655775147054	21.57950952547664
32-33	21.79215159450644	27.597467494049916	28.89485468618564	21.71552622525801
34-35	21.931846953003113	27.643525684454602	28.89042739809219	21.534199964450092
36-37	21.958829865468118	27.562267399936168	28.75245696387897	21.726445770716744
38-39	21.806654975142077	27.621571475243012	28.82188003570892	21.749893513905995
40-41	22.050232820735612	27.530065739116615	28.697065913183025	21.722635526964744
42-43	22.02077402641331	27.600118776887918	28.47668919960331	21.902417997095462
44-45	22.07981090991727	27.754648080158535	28.23190703895933	21.933633970964863
46-47	22.145984892530112	27.74548786203841	27.90303314011094	22.20549410532054
48-49	22.127342400758952	27.82234663659614	27.92485561731663	22.125455345328277
50-51	22.13054660545024	27.924625593663105	27.759678915216956	22.185148885669697
52-53	22.231712079874942	27.874611038210823	27.704871662276915	22.188805219637324
54-55	22.233156883643606	27.801818348140127	27.791041838711987	22.173982929504284
56-57	22.23705240402281	27.873341973566212	27.707742274618933	22.181863347792046
58-59	22.205802333291455	27.92866320334785	27.751693841304647	22.113840622056056
60-61	22.259752462123604	27.91892770857642	27.715552662063132	22.10576716723685
62-63	22.196502558035775	27.97777796769517	27.66172915787415	22.163990316394905
64-65	22.185525038419467	27.891158083628582	27.702576351878943	22.22074052607301
66-67	22.268094048320545	27.961904682098414	27.550644838416776	22.21935643116426
68-69	22.251285710999895	27.892851303862948	27.61531303092109	22.240549954216068
70-71	22.246491209439377	27.655719468299406	27.72746813450989	22.370321187751326
72-73	22.262227957534062	27.728423836026376	27.679573757253806	22.329774449185752
74-75	22.14321793055091	27.819622237826053	27.644032807749337	22.393127023873703
76-77	22.235055125350243	27.77986226480608	27.628254252681312	22.35682835716236
78-79	22.23599688361216	27.82421671622756	27.589498091969244	22.350288308191033
80-81	22.21486717405647	27.80708067845107	27.59660952047461	22.381442627017854
82-83	22.26103758912967	27.77818121209875	27.67542898316106	22.285352215610516
84-85	22.355515023756503	27.77963437833827	27.56835577170923	22.296494826195996
86-87	22.193570279104215	27.791304259306234	27.649031605682694	22.36609385590686
88-89	22.254425177870676	27.763878757204324	27.62464533767038	22.357050727254624
90-91	22.28321594557207	27.794704766808565	27.64680837602429	22.275270911595072
92-93	22.288209203903477	27.794795933859863	27.64012692652054	22.276867935716115
94-95	22.12596844790278	27.88190238681369	27.677506759217508	22.31462240606603
96-97	22.175190202805965	28.023511358828586	27.73082569535373	22.070472743011727
98-99	23.297936884482173	29.9383279632681	27.904923992978386	18.85881115927134
100	22.281067333394383	30.966284201528232	21.587318030031753	25.165330435045636
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4719.0
1	4800.0
2	6093.5
3	7266.0
4	8844.5
5	15360.0
6	21056.0
7	23674.5
8	24099.0
9	21480.0
10	17872.0
11	13805.5
12	13995.5
13	20451.0
14	37718.0
15	64125.5
16	87746.5
17	103118.5
18	108730.5
19	104160.5
20	92348.0
21	78420.5
22	68656.5
23	68590.5
24	78400.0
25	97975.0
26	127608.5
27	164657.0
28	206366.0
29	254983.5
30	306995.0
31	359829.5
32	417124.0
33	473564.0
34	528977.0
35	581256.0
36	633733.5
37	681208.5
38	714952.5
39	745168.5
40	775296.5
41	794594.5
42	804250.0
43	815987.0
44	828012.0
45	836900.5
46	836578.0
47	830196.0
48	820248.0
49	800783.5
50	775712.0
51	738887.0
52	691083.5
53	638049.5
54	581475.0
55	527343.5
56	476531.5
57	424691.0
58	372080.0
59	320934.0
60	267581.5
61	215102.0
62	168394.0
63	129618.0
64	98728.5
65	75156.5
66	57255.0
67	44244.5
68	34260.0
69	25585.5
70	19049.5
71	14472.0
72	10866.0
73	8219.5
74	6111.0
75	4432.0
76	3172.0
77	2233.0
78	1638.0
79	1188.0
80	872.5
81	650.5
82	508.0
83	366.0
84	269.0
85	211.0
86	172.0
87	136.0
88	88.0
89	53.0
90	38.0
91	32.0
92	26.0
93	15.5
94	6.5
95	3.5
96	3.0
97	2.0
98	2.0
99	4.0
100	7.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.012396496088059051
3	0.005335743346459298
4	0.13430430290736406
5	0.06228248207423273
6	0.002785729457990798
7	0.0407948650434614
8	8.571475255356302E-5
9	4.3928810683701046E-4
10-11	2.383941555395971E-4
12-13	1.5803657502063182E-4
14-15	4.553596229408035E-5
16-17	7.955400471377568E-4
18-19	2.0357253731471215E-4
20-21	0.004203090885487513
22-23	0.050820417013988706
24-25	7.198693447883528E-4
26-27	8.067782062636592E-5
28-29	0.001887896287542171
30-31	0.0031146164550909148
32-33	0.00323243944109985
34-35	0.01863835108083825
36-37	0.007977595701672528
38-39	0.0036878486293834595
40-41	0.01212121010947649
42-43	0.003007236905859243
44-45	0.009471606945860092
46-47	0.002633735371475163
48-49	7.026220852475603E-4
50-51	0.03837523589057962
52-53	7.28419614685254E-5
54-55	4.989122246114574E-5
56-57	0.006786533506697968
58-59	3.5768721236918146E-4
60-61	4.5085378180660717E-4
62-63	5.727794998034821E-4
64-65	0.004475438579233684
66-67	2.6494462518699774E-4
68-69	7.397484693578467E-4
70-71	4.0784096210310404E-5
72-73	9.373452469914226E-4
74-75	9.585098162907306E-6
76-77	2.5791893337109267E-5
78-79	3.2550529977963944E-6
80-81	5.853603353259702E-4
82-83	0.0
84-85	2.350599031694235E-5
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	3.922933752454821E-5
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	9207.0
20-21	18203.0
22-23	19769.0
24-25	20958.0
26-27	25156.0
28-29	34783.0
30-31	54115.0
32-33	63099.0
34-35	78036.0
36-37	102967.0
38-39	131555.0
40-41	133088.0
42-43	151880.0
44-45	180529.0
46-47	170504.0
48-49	148742.0
50-51	132864.0
52-53	122998.0
54-55	126689.0
56-57	133378.0
58-59	137529.0
60-61	139047.0
62-63	131377.0
64-65	134919.0
66-67	142466.0
68-69	146878.0
70-71	150873.0
72-73	141354.0
74-75	139115.0
76-77	145689.0
78-79	154490.0
80-81	160495.0
82-83	155882.0
84-85	153604.0
86-87	159346.0
88-89	170434.0
90-91	183773.0
92-93	190753.0
94-95	216987.0
96-97	603528.0
98-99	1182611.0
100-101	1.2066895E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.21189337488924
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.4713503262432	73.61868570079821
2	7.933915370822489	13.203922397645515
3	1.9941945268855379	4.978221070199611
4	0.7439587522934443	2.476248654846309
5	0.34936803186174337	1.453578770793715
6	0.17505846984038906	0.8740168036037832
7	0.10228489880971166	0.5957924065530542
8	0.06011489388158568	0.40018193119338313
9	0.041964866974247055	0.3142778432537211
>10	0.12244012073532518	1.565779458772753
>50	0.0037318996957884016	0.21777685581222642
>100	0.0015466795329117228	0.2524016401608149
>500	4.749001955617292E-5	0.023755077004073526
>1k	2.3672404109857193E-5	0.025361389362874943
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0714344069195377E-5
2	0.0	3.214303220758613E-5	0.0	0.0	1.0714344069195377E-5
3	0.0	3.214303220758613E-5	0.0	5.357172034597689E-6	1.0714344069195377E-5
4	0.0	1.0178626865735608E-4	0.0	2.1428688138390754E-5	1.0714344069195377E-5
5	0.0	1.1250061272655146E-4	0.0	2.1428688138390754E-5	1.0714344069195377E-5
6	0.0	1.1785778476114915E-4	0.0	2.1428688138390754E-5	2.1428688138390754E-5
7	0.0	1.1785778476114915E-4	0.0	3.750020424218382E-5	2.6785860172988442E-5
8	0.0	1.4464364493413758E-4	0.0	4.285737627678151E-5	2.6785860172988442E-5
9	0.0	2.0892970934930985E-4	0.0	9.107192458816071E-5	3.214303220758613E-5
10-11	0.0	2.0892970934930985E-4	0.0	9.107192458816071E-5	3.214303220758613E-5
12-13	0.0	2.0892970934930985E-4	0.0	1.2857212883034453E-4	3.214303220758613E-5
14-15	0.0	2.8660870385097635E-4	0.0	1.526794029860341E-4	3.7500204242183824E-5
16-17	0.0	2.973230479201717E-4	0.0	1.687509190898272E-4	4.553596229408035E-5
18-19	0.0	3.321446661450567E-4	0.0	2.0357253731471215E-4	6.160747839787342E-5
20-21	0.0	3.61609112335344E-4	0.0	2.2767981147040178E-4	8.035758051896533E-5
22-23	0.0	4.044664886121255E-4	0.0	2.3839415553959716E-4	8.303616653626417E-5
24-25	1.3392930086494221E-5	4.47323864888907E-4	0.0	2.544656716433902E-4	9.107192458816071E-5
26-27	1.6071516103793067E-5	4.660739670099989E-4	0.0	2.946444619028729E-4	9.107192458816071E-5
28-29	2.4107274155689597E-5	4.714311390445966E-4	0.0	4.1518083268132083E-4	9.910768264005725E-5
30-31	2.6785860172988442E-5	5.598244776154585E-4	0.0	0.0010526843047984457	1.1250061272655146E-4
32-33	3.750020424218382E-5	5.625030636327573E-4	0.0	0.0022767981147040176	1.2589354281304567E-4
34-35	3.750020424218382E-5	6.616107462728146E-4	0.0	0.0039375214454293005	1.5000081696873527E-4
36-37	3.750020424218382E-5	7.607184289128718E-4	0.0	0.006672357769091421	1.5000081696873527E-4
38-39	3.750020424218382E-5	8.035758051896532E-4	0.0	0.01114827500399779	1.5000081696873527E-4
40-41	3.750020424218382E-5	9.267907619854E-4	0.0	0.016264374297038583	1.5000081696873527E-4
42-43	3.750020424218382E-5	9.45540864106492E-4	0.0	0.020670648295495184	1.526794029860341E-4
44-45	3.750020424218382E-5	0.0010419699607292503	0.0	0.02560192515334235	1.5803657502063182E-4
46-47	3.750020424218382E-5	0.001114291783196319	0.0	0.03101266890828602	1.7142950510712604E-4
48-49	3.750020424218382E-5	0.0011250061272655145	0.0	0.03623055446998416	1.875010212109191E-4
50-51	3.750020424218382E-5	0.0012455424980439625	0.0	0.04159308367661645	1.9285819324551677E-4
52-53	3.750020424218382E-5	0.0012535782560958591	0.0	0.04716186400658075	2.169654674012064E-4
54-55	3.750020424218382E-5	0.0012696497721996522	0.0	0.05220028430511987	2.3839415553959716E-4
56-57	3.750020424218382E-5	0.0013071499764418359	0.0	0.05697352458794642	2.464299135914937E-4
58-59	3.750020424218382E-5	0.0013098285624591347	0.0	0.06186997982756871	2.5178708562609135E-4
60-61	3.750020424218382E-5	0.0013419715946667208	0.0	0.06690572154009053	2.5714425766068907E-4
62-63	3.750020424218382E-5	0.001358043110770514	0.0	0.07155574686612132	2.705371877471833E-4
64-65	3.750020424218382E-5	0.001516079685791146	0.0	0.07590309197219736	2.732157737644821E-4
66-67	3.750020424218382E-5	0.0015348297879122378	0.0	0.08029329445455016	2.758943597817809E-4
68-69	3.750020424218382E-5	0.0015750085781717203	0.0	0.08473171148521433	2.8928728986827516E-4
70-71	3.750020424218382E-5	0.0016071516103793064	0.0	0.0890120919408579	3.0000163393747054E-4
72-73	3.750020424218382E-5	0.0016232231264830996	0.0	0.09212996606499374	3.214303220758613E-4
74-75	3.750020424218382E-5	0.001671437674794479	0.0	0.0947871233941542	3.2410890809316016E-4
76-77	3.750020424218382E-5	0.0017437594972615476	0.0	0.09720588656777505	3.2678749411045897E-4
78-79	3.750020424218382E-5	0.00178393828752103	0.0	0.0987219662535662	3.321446661450567E-4
80-81	3.750020424218382E-5	0.001789295459555628	0.0	0.09924161194092218	3.508947682661486E-4
82-83	3.750020424218382E-5	0.0017946526315902257	0.0	0.0996192925693613	3.6428769835264283E-4
84-85	3.750020424218382E-5	0.001805366975659421	0.0	0.09975857904226085	3.6428769835264283E-4
86-87	3.750020424218382E-5	0.001824117077780513	0.0	0.09983090086472793	3.696448703872405E-4
88	3.750020424218382E-5	0.001944653448558961	0.0	0.09983090086472793	3.750020424218382E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	29470	0.0	48.05958	1
GTATCAA	51660	0.0	36.158062	1
ATCAACG	62110	0.0	29.49415	3
TCAACGC	62380	0.0	29.36586	4
CAACGCA	63435	0.0	28.857164	5
TATCAAC	65085	0.0	28.302353	2
AACGCAG	65605	0.0	27.924753	6
ACGCAGA	75005	0.0	24.373575	7
CGCAGAG	75905	0.0	24.069004	8
GCAGAGT	88925	0.0	20.273724	9
GTACATG	73270	0.0	19.35903	1
TACATGG	72160	0.0	19.1867	2
GAGTACT	43995	0.0	18.950657	12-13
ACATGGG	72980	0.0	18.610872	3
CAGAGTA	86495	0.0	18.292425	10-11
AGAGTAC	81025	0.0	17.83545	10-11
GTACTTT	49285	0.0	16.91224	14-15
CATGGGG	44350	0.0	16.569494	4
AGTACTT	47915	0.0	15.899107	12-13
GTGGTAT	12685	0.0	15.176759	1
>>END_MODULE