FastQCFastQC Report
Fri 27 May 2016
SRR2139905_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139905_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38480000
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA564550.048.9838181
GTATCAA996100.036.1169551
ATCAACG1204350.029.3203033
TCAACGC1204400.029.3149934
CAACGCA1222500.028.8357645
TATCAAC1254800.028.3053572
AACGCAG1273600.027.783126
ACGCAGA1455550.024.2506627
CGCAGAG1476350.023.8935158
CTTATAC227250.021.0829451
GCAGAGT1710300.020.3201089
GTACATG1395850.019.9925881
TACATGG1388350.019.72712
GAGTACT866750.019.03441412-13
ACATGGG1407500.018.9502583
CAGAGTA1667800.018.28676210-11
TATACAC371000.018.262153
AGAGTAC1557050.018.0494710-11
CATGGGG930500.016.819014
GTACTTT976650.016.81820114-15
Produced by FastQC (version 0.11.5)