##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139905_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	38480000
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.795337889812888	33.0	31.0	34.0	30.0	34.0
2	31.83594022869023	33.0	31.0	34.0	29.0	34.0
3	31.914919074844075	34.0	31.0	34.0	29.0	34.0
4	35.4212501039501	37.0	35.0	37.0	33.0	37.0
5	35.25476364345114	37.0	35.0	37.0	32.0	37.0
6	35.320464682952185	37.0	35.0	37.0	32.0	37.0
7	35.22660337837838	37.0	35.0	37.0	32.0	37.0
8	35.23421670997921	37.0	35.0	37.0	32.0	37.0
9	36.81450537941788	39.0	37.0	39.0	32.0	39.0
10-11	36.772443672037426	39.0	37.0	39.0	32.0	39.0
12-13	36.738898102910596	39.0	37.0	39.0	32.0	39.0
14-15	37.98673119802495	40.0	38.0	41.0	32.0	41.0
16-17	38.00835888773389	40.0	38.0	41.0	32.0	41.0
18-19	38.037362383056134	40.0	38.0	41.0	32.0	41.0
20-21	38.08029123119706	40.0	38.0	41.0	32.0	41.0
22-23	38.00862336593366	40.0	38.0	41.0	32.0	41.0
24-25	38.022180622530215	40.0	38.0	41.0	32.0	41.0
26-27	37.95714868594164	40.0	37.5	41.0	32.0	41.0
28-29	37.89851419959774	40.0	37.0	41.0	32.0	41.0
30-31	37.85231875981821	40.0	37.0	41.0	32.0	41.0
32-33	37.75567507426072	40.0	37.0	41.0	32.0	41.0
34-35	37.67039602253722	40.0	37.0	41.0	31.0	41.0
36-37	37.61869613857266	40.0	37.0	41.0	31.0	41.0
38-39	37.59488086568871	40.0	37.0	41.0	31.0	41.0
40-41	37.553584332045645	40.0	37.0	41.0	31.0	41.0
42-43	37.51629694341982	40.0	37.0	41.0	31.0	41.0
44-45	37.45506625871475	40.0	36.0	41.0	31.0	41.0
46-47	37.412860330683216	40.0	36.0	41.0	31.0	41.0
48-49	37.347747354607165	40.0	36.0	41.0	31.0	41.0
50-51	37.25421861416452	40.0	36.0	41.0	31.0	41.0
52-53	37.17002964534434	39.5	36.0	41.0	31.0	41.0
54-55	37.20445037682609	39.0	35.5	41.0	31.0	41.0
56-57	37.2736897346005	39.0	36.0	41.0	31.0	41.0
58-59	37.17428612009376	39.0	35.0	41.0	31.0	41.0
60-61	37.03545040803627	39.0	35.0	41.0	31.0	41.0
62-63	36.8424243992566	39.0	35.0	41.0	31.0	41.0
64-65	36.63221419252841	38.0	35.0	40.5	31.0	41.0
66-67	36.3815162053412	38.0	35.0	40.0	30.0	41.0
68-69	36.134750734111	37.0	35.0	40.0	30.0	41.0
70-71	35.85031561209017	37.0	34.0	39.5	30.0	41.0
72-73	35.5788763931246	36.0	34.0	39.0	30.0	41.0
74-75	35.289169813242005	36.0	34.0	39.0	30.0	40.5
76-77	34.98496588568095	35.0	34.0	37.5	30.0	40.0
78-79	34.67732196704472	35.0	34.0	37.0	29.0	39.0
80-81	34.407517784265515	35.0	34.0	37.0	29.0	39.0
82-83	34.162866837536214	35.0	34.0	36.0	29.0	38.5
84-85	33.95102010097952	35.0	34.0	36.0	29.0	37.0
86-87	33.74733387818931	35.0	33.5	36.0	29.0	37.0
88-89	33.616109879304354	35.0	33.0	35.0	29.0	36.5
90-91	33.51308288163557	35.0	33.0	35.0	29.0	36.0
92-93	33.44242287846388	35.0	33.0	35.0	29.0	36.0
94-95	33.50367613837413	35.0	33.5	35.0	29.5	36.0
96-97	33.67508586693066	35.0	34.0	35.0	30.0	36.0
98-99	33.75497581317576	35.0	34.0	35.0	30.0	35.0
100	33.08593285753829	35.0	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	3.0
14	13.0
15	35.0
16	86.0
17	436.0
18	3536.0
19	18374.0
20	56151.0
21	121894.0
22	201834.0
23	276651.0
24	345834.0
25	412074.0
26	473948.0
27	533643.0
28	601574.0
29	684241.0
30	790132.0
31	927608.0
32	1120968.0
33	1394436.0
34	1830058.0
35	2487176.0
36	3787150.0
37	6892766.0
38	9949254.0
39	5335361.0
40	234763.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.99940228690229	21.288386174636177	14.544339916839915	24.16787162162162
2	14.470457180566063	22.347984427359254	40.53987025918274	22.64168813289194
3	17.1276453468835	27.983016512930583	31.371576776932887	23.517761363253026
4	12.237694379394268	19.7117521453373	39.484628266095314	28.56592520917312
5	12.503746824576737	38.844110176392455	35.92703610580193	12.725106893228885
6	27.97356713135671	39.72126965859299	19.52374639708489	12.781416812965418
7	24.896922622395806	33.673695624525465	24.30523122890967	17.124150524169053
8	24.280780364433756	36.47412666901768	21.80794059870629	17.43715236784228
9	25.85195672642005	16.219944318880827	21.15644481797347	36.77165413672566
10-11	23.750336669064108	26.896917727800083	29.594801174763237	19.75794442837257
12-13	24.9706464023304	24.61838244024791	29.33890430369831	21.072066853723385
14-15	22.235125320978515	25.696053975066	26.93266032553543	25.13616037842006
16-17	21.545805541171728	28.77830584629975	27.49905635430397	22.17683225822455
18-19	21.68087492181149	27.94281515954874	29.134738162192313	21.24157175644746
20-21	22.731381822611354	27.022933171121633	28.82815347862345	21.417531527643565
22-23	22.377678464581845	27.096667838690426	28.63982536029832	21.885828336429412
24-25	22.02773917396571	27.338109478806572	28.758414520369147	21.87573682685857
26-27	22.063069489004114	27.38910777487656	28.690143387125406	21.857679348993923
28-29	21.960338613771647	27.31992391267929	28.757977096756775	21.96176037679229
30-31	22.24660236035303	27.289180455331884	28.566489912629457	21.89772727168563
32-33	22.17673414067375	27.380300294790345	28.519756820584718	21.923208743951182
34-35	22.217278342823104	27.381941943294773	28.534334667012374	21.86644504686975
36-37	22.16742235302494	27.44677001066887	28.44418498626313	21.94162265004306
38-39	22.10923556763524	27.373091222417166	28.511687160887323	22.005986049060272
40-41	22.284511663355254	27.27839768230188	28.42644968660234	22.010640967740525
42-43	22.23670232600848	27.355988342367617	28.23212271121645	22.17518662040745
44-45	22.2884358725491	27.51152480652831	28.006727003557362	22.193312317365223
46-47	22.42415277121681	27.498878213091793	27.63518606043769	22.441782955253707
48-49	22.378071608908552	27.58402906830881	27.633596787813953	22.404302534968686
50-51	22.337666626422237	27.68600946174782	27.524143038656483	22.452180873173464
52-53	22.460435066953348	27.58883363438404	27.56905778736179	22.381673511300825
54-55	22.420938660222934	27.514248515654792	27.61133593535776	22.453476888764513
56-57	22.46980423640777	27.636823813107615	27.49703079272739	22.396341157757227
58-59	22.412679269070793	27.724487388285713	27.56389955866106	22.298933783982438
60-61	22.52929626655356	27.774198348217222	27.413942630877465	22.282562754351755
62-63	22.406125819464105	27.885262075520473	27.384651308603324	22.323960796412102
64-65	22.367269516908813	27.819465769881184	27.40185634408107	22.41140836912893
66-67	22.46552479663537	27.80964733303564	27.292853201639627	22.431974668689357
68-69	22.4146771336242	27.705497526833543	27.39375520295762	22.486070136584637
70-71	22.476625038439284	27.451252940326516	27.481461061906398	22.590660959327803
72-73	22.470652455283364	27.548671916035378	27.433365632937946	22.547309995743312
74-75	22.322541609300973	27.669356349609835	27.417357241511237	22.590744799577955
76-77	22.443408542797812	27.62005612634399	27.409356583040324	22.527178747817878
78-79	22.43047531644048	27.644077713627713	27.34456381611206	22.580883153819748
80-81	22.46396019860696	27.632497529644073	27.348916161223762	22.554626110525202
82-83	22.46686548259003	27.59098338732393	27.475723443157683	22.466427686928352
84-85	22.52182207275285	27.602877529089476	27.38519077209915	22.490109626058523
86-87	22.393555167516308	27.623045643625904	27.468662350247268	22.514736838610524
88-89	22.46746077478682	27.62800822084501	27.368289470611177	22.536241533756993
90-91	22.501004370834572	27.598193314592063	27.45059689048951	22.450205424083858
92-93	22.50603462852732	27.602360816224998	27.433065359364413	22.458539195883272
94-95	22.30516740661156	27.674774887181485	27.48130533545721	22.538752370749748
96-97	22.364006604247688	27.825257388992085	27.535838396569407	22.274897610190816
98-99	23.504918524534126	29.750604205639043	27.67866101764928	19.065816252177548
100	22.476799732740012	30.790542645165438	21.3992484945921	25.33340912750245
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6230.0
1	6470.0
2	10372.5
3	14090.5
4	17573.0
5	30715.0
6	42011.5
7	47324.0
8	48206.0
9	42905.0
10	35711.0
11	27912.5
12	28690.5
13	41755.5
14	76279.5
15	128244.0
16	173633.0
17	202358.5
18	212350.0
19	203165.5
20	180659.5
21	154275.5
22	135475.0
23	135453.0
24	155806.5
25	195140.0
26	252794.5
27	322936.5
28	402041.0
29	494735.0
30	592513.5
31	693587.0
32	804824.5
33	916167.5
34	1023690.0
35	1120279.0
36	1221371.0
37	1323103.0
38	1393967.0
39	1445009.5
40	1498641.5
41	1539493.0
42	1567072.5
43	1600860.5
44	1644638.0
45	1685612.5
46	1691181.5
47	1683365.5
48	1671495.5
49	1631161.5
50	1581743.5
51	1519744.5
52	1436857.0
53	1344468.5
54	1247735.0
55	1147942.5
56	1052408.5
57	954660.0
58	849111.5
59	738296.5
60	617876.5
61	503075.5
62	401266.5
63	311705.0
64	236541.0
65	178504.5
66	135610.5
67	105291.5
68	82010.5
69	61290.0
70	45318.0
71	34448.0
72	26125.5
73	19291.5
74	13728.5
75	9734.0
76	6914.5
77	4828.5
78	3510.0
79	2609.0
80	1958.0
81	1489.0
82	1149.5
83	783.5
84	537.0
85	406.5
86	317.5
87	237.0
88	148.5
89	97.5
90	73.0
91	59.0
92	46.5
93	29.0
94	15.0
95	9.0
96	8.5
97	9.5
98	11.0
99	13.0
100	25.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.012551975051975053
3	0.005597713097713098
4	0.13395010395010395
5	0.06263773388773389
6	0.00283004158004158
7	0.04077962577962578
8	7.796257796257797E-5
9	4.417879417879418E-4
10-11	1.975051975051975E-4
12-13	1.2733887733887734E-4
14-15	2.598752598752599E-5
16-17	6.50987525987526E-4
18-19	2.1179833679833677E-4
20-21	0.004123525693271171
22-23	0.051118131674725176
24-25	6.411965332015215E-4
26-27	8.611957337407224E-5
28-29	0.0019248314508207329
30-31	0.003236961575734039
32-33	0.003246145907561399
34-35	0.01853852620231815
36-37	0.00784830560002995
38-39	0.0035998194903961484
40-41	0.012032309058322666
42-43	0.0030701002772345006
44-45	0.009462034012814818
46-47	0.0026236335595805124
48-49	6.796675745892684E-4
50-51	0.03839937509999257
52-53	5.0434268471529816E-5
54-55	5.6392045394468706E-5
56-57	0.006716846271938156
58-59	3.0724162215688247E-4
60-61	4.10277913765312E-4
62-63	5.162568558837954E-4
64-65	0.004292614518016653
66-67	2.3254695715470966E-4
68-69	6.929438697956391E-4
70-71	3.292763493669962E-5
72-73	8.436937109893536E-4
74-75	9.126488707133852E-6
76-77	2.1463810734937898E-5
78-79	3.0921073696560405E-6
80-81	5.709879256490725E-4
82-83	0.0
84-85	3.0194753458169356E-5
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	3.858217485740238E-5
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	20329.0
20-21	39858.0
22-23	43418.0
24-25	45136.0
26-27	52664.0
28-29	69599.0
30-31	104129.0
32-33	119491.0
34-35	145641.0
36-37	183766.0
38-39	234698.0
40-41	240798.0
42-43	274204.0
44-45	328947.0
46-47	313530.0
48-49	273184.0
50-51	244346.0
52-53	224277.0
54-55	229758.0
56-57	242153.0
58-59	251635.0
60-61	258222.0
62-63	244982.0
64-65	256698.0
66-67	273439.0
68-69	282876.0
70-71	288736.0
72-73	258735.0
74-75	256102.0
76-77	267795.0
78-79	286391.0
80-81	300701.0
82-83	290388.0
84-85	285982.0
86-87	297164.0
88-89	323730.0
90-91	353484.0
92-93	368933.0
94-95	430636.0
96-97	1255237.0
98-99	2499431.0
100-101	2.5718777E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.95916913641192
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.02929821631828	66.20743312116457
2	8.606406345175891	13.2468376315016
3	2.5150497101382974	5.806684080870509
4	1.10063801260433	3.388167478799235
5	0.5788245753117132	2.2272929195862967
6	0.34491856497740114	1.5926787708230576
7	0.2204858367175672	1.1877884760091388
8	0.14388832473730098	0.8858820736170326
9	0.10212516779565317	0.7073521255323106
>10	0.3499839157802085	4.069104813599466
>50	0.00637040632305952	0.32757843710443446
>100	0.0019084452024950161	0.24259727944036924
>500	6.013098330892416E-5	0.03315131131530672
>1k	4.234793435320189E-5	0.07745148063666
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.2993762993762994E-5	0.0	0.0	0.0
3	0.0	1.5592515592515594E-5	0.0	2.5987525987525987E-6	0.0
4	0.0	3.638253638253638E-5	0.0	5.1975051975051975E-6	0.0
5	0.0	3.638253638253638E-5	0.0	5.1975051975051975E-6	0.0
6	0.0	3.638253638253638E-5	0.0	1.2993762993762994E-5	2.5987525987525987E-6
7	0.0	3.638253638253638E-5	0.0	2.079002079002079E-5	5.1975051975051975E-6
8	0.0	4.4178794178794176E-5	0.0	2.598752598752599E-5	7.796257796257797E-6
9	2.5987525987525987E-6	8.835758835758835E-5	0.0	6.756756756756757E-5	7.796257796257797E-6
10-11	1.2993762993762994E-5	8.835758835758835E-5	0.0	8.056133056133056E-5	7.796257796257797E-6
12-13	1.2993762993762994E-5	8.835758835758835E-5	0.0	8.445945945945946E-5	9.095634095634097E-6
14-15	1.9490644490644492E-5	1.3773388773388772E-4	0.0	9.485446985446985E-5	1.559251559251559E-5
16-17	3.2484407484407486E-5	1.4293139293139294E-4	0.0	1.4163201663201663E-4	2.2089397089397088E-5
18-19	3.508316008316008E-5	1.5462577962577963E-4	0.0	1.5592515592515593E-4	2.338877338877339E-5
20-21	4.4178794178794176E-5	1.741164241164241E-4	0.0	1.8451143451143453E-4	2.7286902286902287E-5
22-23	4.4178794178794176E-5	2.091995841995842E-4	0.0	2.053014553014553E-4	3.2484407484407486E-5
24-25	4.4178794178794176E-5	2.481808731808732E-4	0.0	2.312889812889813E-4	3.3783783783783784E-5
26-27	4.4178794178794176E-5	2.6507276507276505E-4	0.0	2.7676715176715177E-4	3.3783783783783784E-5
28-29	4.4178794178794176E-5	2.6507276507276505E-4	0.0	4.378898128898129E-4	3.3783783783783784E-5
30-31	4.4178794178794176E-5	3.0405405405405404E-4	0.0	9.576403326403327E-4	3.508316008316008E-5
32-33	4.4178794178794176E-5	3.079521829521829E-4	0.0	0.002014033264033264	3.8981288981288983E-5
34-35	4.4178794178794176E-5	3.586278586278586E-4	0.0	0.0034537422037422038	3.8981288981288983E-5
36-37	4.4178794178794176E-5	4.119022869022869E-4	0.0	0.005560031185031185	3.8981288981288983E-5
38-39	4.4178794178794176E-5	4.170997920997921E-4	0.0	0.009250259875259875	4.158004158004158E-5
40-41	4.4178794178794176E-5	4.5997920997920997E-4	0.0	0.013486226611226611	4.4178794178794176E-5
42-43	4.4178794178794176E-5	4.807692307692308E-4	0.0	0.017242723492723492	4.4178794178794176E-5
44-45	4.4178794178794176E-5	5.47037422037422E-4	0.0	0.021057692307692305	4.547817047817048E-5
46-47	4.4178794178794176E-5	5.899168399168399E-4	0.0	0.025417099792099794	4.937629937629938E-5
48-49	4.677754677754678E-5	5.925155925155924E-4	0.0	0.02989345114345114	5.4573804573804574E-5
50-51	4.677754677754678E-5	6.834719334719334E-4	0.0	0.034377598752598756	5.4573804573804574E-5
52-53	4.677754677754678E-5	6.886694386694387E-4	0.0	0.03876299376299376	5.8471933471933475E-5
54-55	4.677754677754678E-5	7.029625779625779E-4	0.0	0.042900207900207904	6.237006237006238E-5
56-57	4.677754677754678E-5	7.12058212058212E-4	0.0	0.04627988565488565	6.366943866943867E-5
58-59	4.677754677754678E-5	7.12058212058212E-4	0.0	0.049699844074844075	6.626819126819128E-5
60-61	4.677754677754678E-5	7.185550935550936E-4	0.0	0.05342385654885655	7.016632016632016E-5
62-63	4.677754677754678E-5	7.302494802494802E-4	0.0	0.056803534303534305	7.276507276507276E-5
64-65	4.677754677754678E-5	8.225051975051975E-4	0.0	0.0599285343035343	7.406444906444905E-5
66-67	4.677754677754678E-5	8.290020790020791E-4	0.0	0.06318607068607068	7.536382536382536E-5
68-69	4.677754677754678E-5	8.614864864864865E-4	0.0	0.06652546777546778	7.666320166320166E-5
70-71	4.677754677754678E-5	8.796777546777547E-4	0.0	0.0693866943866944	8.056133056133056E-5
72-73	4.677754677754678E-5	8.848752598752599E-4	0.0	0.07163591476091476	8.835758835758835E-5
74-75	4.677754677754678E-5	9.043659043659043E-4	0.0	0.07353820166320166	8.835758835758835E-5
76-77	4.677754677754678E-5	9.381496881496882E-4	0.0	0.07522089397089397	8.835758835758835E-5
78-79	4.677754677754678E-5	9.511434511434512E-4	0.0	0.07631366943866943	8.835758835758835E-5
80-81	4.677754677754678E-5	9.511434511434512E-4	0.0	0.07669308731808733	9.225571725571725E-5
82-83	4.677754677754678E-5	9.563409563409563E-4	0.0	0.07693347193347193	9.875259875259875E-5
84-85	4.677754677754678E-5	9.641372141372141E-4	0.0	0.07702053014553015	9.875259875259875E-5
86-87	4.677754677754678E-5	9.953222453222452E-4	0.0	0.07705301455301455	9.875259875259875E-5
88	4.677754677754678E-5	0.0010654885654885655	0.0	0.07705821205821206	1.0395010395010396E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	56455	0.0	48.983818	1
GTATCAA	99610	0.0	36.116955	1
ATCAACG	120435	0.0	29.320303	3
TCAACGC	120440	0.0	29.314993	4
CAACGCA	122250	0.0	28.835764	5
TATCAAC	125480	0.0	28.305357	2
AACGCAG	127360	0.0	27.78312	6
ACGCAGA	145555	0.0	24.250662	7
CGCAGAG	147635	0.0	23.893515	8
CTTATAC	22725	0.0	21.082945	1
GCAGAGT	171030	0.0	20.320108	9
GTACATG	139585	0.0	19.992588	1
TACATGG	138835	0.0	19.7271	2
GAGTACT	86675	0.0	19.034414	12-13
ACATGGG	140750	0.0	18.950258	3
CAGAGTA	166780	0.0	18.286762	10-11
TATACAC	37100	0.0	18.26215	3
AGAGTAC	155705	0.0	18.04947	10-11
CATGGGG	93050	0.0	16.81901	4
GTACTTT	97665	0.0	16.818201	14-15
>>END_MODULE