FastQCFastQC Report
Fri 27 May 2016
SRR2139904_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139904_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11600696
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA142800.049.603541
GTATCAA251600.037.4982381
TCAACGC300400.030.923024
ATCAACG302800.030.4953863
CAACGCA309700.029.9902925
TATCAAC318350.029.2873022
AACGCAG320200.029.0009776
ACGCAGA372750.024.9238837
CGCAGAG379500.024.4693368
GTGGTAT66750.022.6939351
GCAGAGT446100.020.7970899
GTACATG324750.020.734361
TGGTATC67300.020.6697542
TACATGG324050.020.4762822
GAGTACT262000.020.23696112-13
ACATGGG328550.019.4425453
CAGAGTA429350.018.87136510-11
GTACTTT289250.018.36026814-15
AGAGTAC398850.018.21830710-11
CATGGGG251250.017.560544
Produced by FastQC (version 0.11.5)