##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139904_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11600696
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.65110851969572	33.0	33.0	33.0	33.0	33.0
2	32.67475908342051	33.0	33.0	33.0	33.0	33.0
3	32.579106374307194	33.0	33.0	33.0	33.0	33.0
4	36.54870638796155	37.0	37.0	37.0	37.0	37.0
5	36.600283638154124	37.0	37.0	37.0	37.0	37.0
6	36.60943395120431	37.0	37.0	37.0	37.0	37.0
7	36.588906562157995	37.0	37.0	37.0	37.0	37.0
8	36.58829030602991	37.0	37.0	37.0	37.0	37.0
9	36.59398461954352	37.0	37.0	37.0	37.0	37.0
10-11	36.5549283422305	37.0	37.0	37.0	37.0	37.0
12-13	36.524607316664444	37.0	37.0	37.0	37.0	37.0
14-15	38.569312694686595	40.0	37.0	40.0	37.0	40.0
16-17	38.51062660378308	40.0	37.0	40.0	37.0	40.0
18-19	38.513375921582636	40.0	37.0	40.0	37.0	40.0
20-21	38.53936323675876	40.0	37.0	40.0	37.0	40.0
22-23	38.58542595103174	40.0	37.0	40.0	37.0	40.0
24-25	38.61517696385788	40.0	37.0	40.0	37.0	40.0
26-27	38.321906307934945	40.0	37.0	40.0	37.0	40.0
28-29	38.28287159320241	40.0	37.0	40.0	37.0	40.0
30-31	38.292165893306105	40.0	37.0	40.0	37.0	40.0
32-33	38.283868858403864	40.0	37.0	40.0	37.0	40.0
34-35	38.10804976987441	40.0	37.0	40.0	37.0	40.0
36-37	38.15840421453784	40.0	37.0	40.0	37.0	40.0
38-39	38.08046794468418	40.0	37.0	40.0	37.0	40.0
40-41	38.07110077404196	40.0	37.0	40.0	35.0	40.0
42-43	38.013292439731515	40.0	37.0	40.0	33.0	40.0
44-45	37.92327435200224	40.0	37.0	40.0	33.0	40.0
46-47	37.83257314670112	40.0	37.0	40.0	33.0	40.0
48-49	37.59539676694977	40.0	37.0	40.0	33.0	40.0
50-51	37.68176336452952	40.0	37.0	40.0	33.0	40.0
52-53	37.64032292845589	40.0	37.0	40.0	33.0	40.0
54-55	33.15409152189058	37.0	33.0	40.0	10.5	40.0
56-57	36.29692393042154	37.0	35.0	40.0	30.0	40.0
58-59	37.06428208406882	37.0	37.0	40.0	33.0	40.0
60-61	37.08400237055636	37.0	37.0	40.0	33.0	40.0
62-63	36.83187991071395	37.0	37.0	40.0	33.0	40.0
64-65	31.982619561625405	37.0	33.0	40.0	8.5	40.0
66-67	34.9584522010634	37.0	33.0	40.0	27.0	40.0
68-69	35.79759406200633	37.0	33.0	37.0	33.0	40.0
70-71	35.69772251097872	37.0	33.0	37.0	33.0	40.0
72-73	35.07691018942728	37.0	33.0	37.0	27.0	40.0
74-75	35.08724197247659	37.0	33.0	37.0	27.0	40.0
76-77	33.27905651423686	35.0	33.0	37.0	27.0	37.0
78-79	34.87040336999691	37.0	33.0	37.0	33.0	37.0
80-81	35.12493764661622	37.0	33.0	37.0	33.0	37.0
82-83	35.07075225007256	37.0	33.0	37.0	33.0	37.0
84-85	34.94759090979404	37.0	33.0	37.0	33.0	37.0
86-87	34.83405101169036	37.0	33.0	37.0	33.0	37.0
88-89	34.73535683536569	37.0	33.0	37.0	33.0	37.0
90-91	34.62175177756876	37.0	33.0	37.0	33.0	37.0
92-93	34.5382864722394	37.0	33.0	37.0	33.0	37.0
94-95	34.475502013407365	37.0	33.0	37.0	33.0	37.0
96-97	34.43992483368157	37.0	33.0	37.0	33.0	37.0
98-99	34.416479838979896	37.0	33.0	37.0	33.0	37.0
100	34.374216716688224	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	8.0
20	78.0
21	404.0
22	1529.0
23	4449.0
24	10743.0
25	22122.0
26	39145.0
27	63626.0
28	94829.0
29	133897.0
30	182569.0
31	245473.0
32	327624.0
33	448254.0
34	643585.0
35	1049890.0
36	2000334.0
37	3059986.0
38	3181461.0
39	90687.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.91068363484398	19.960664429099772	14.495768184943387	23.632883751112864
2	14.854738026063263	20.54475007361627	41.49617402266209	23.104337877658377
3	18.97505099633692	24.959968856093305	29.940011256563707	26.124968891006066
4	12.743484870664707	17.541549181999805	38.2987771938963	31.41618875343919
5	13.742999044827581	37.45710496456016	35.20514308489741	13.594752905714852
6	31.00042445729118	37.1747953743465	18.26684364455374	13.557936523808573
7	27.4068211079749	32.34016303849355	22.14083534298287	18.11218051054868
8	24.808640791897314	36.8403585440046	20.489132721002257	17.861867943095827
9	25.436887579848655	17.130006682357678	20.96239742856808	36.470708309225586
10-11	24.658175681872883	26.784306734699364	28.2322672708603	20.32525031256745
12-13	25.283439890158316	24.05067764899623	28.64045398655391	22.02542847429154
14-15	22.203723811054097	25.686726037817042	26.332980366005625	25.776569785123236
16-17	21.919844292101097	28.85950980872182	27.013060250867703	22.207585648309376
18-19	21.894341511923077	27.941599366107	28.816477907877253	21.347581214092674
20-21	22.652683292038216	27.059023826030774	28.84139980005073	21.44689308188028
22-23	22.359918816568506	26.979180204243097	28.698769769710875	21.96213120947752
24-25	22.12505815932672	27.08539772356894	28.77613117088133	22.013412946223017
26-27	22.121481471766046	27.292771284048218	28.571742291404046	22.014004952781693
28-29	22.020391630066715	27.38319478084556	28.684839280237078	21.911574308850646
30-31	22.31982699085347	27.185179661266073	28.56476116675259	21.930232181127867
32-33	22.088751536255455	27.33453249432471	28.550774054552413	22.02594191486742
34-35	22.282778312880232	27.368593350756438	28.473348788592347	21.87527954777099
36-37	22.22745654258846	27.309016599340275	28.46216335171803	22.001363506353233
38-39	22.073949695719136	27.454125548330765	28.529523692185567	21.94240106376453
40-41	22.390253800817405	27.31165391587983	28.32019683453379	21.977895448768976
42-43	22.239187769118036	27.40983523538741	28.159995913277015	22.190981082217544
44-45	22.37054328822871	27.498934352074016	27.982442831634586	22.148079528062688
46-47	22.395187964297445	27.532295691807267	27.713904773367815	22.35861157052747
48-49	22.33837308542579	27.652594374849855	27.675547545575103	22.333484994149256
50-51	22.329889468105186	27.774442985253906	27.599524301550222	22.29614324509069
52-53	22.511073364241234	27.63413921700347	27.50002010936276	22.354767309392543
54-55	23.4302731127763	27.478928057050915	26.927607333671638	22.16319149650115
56-57	22.437282349306383	27.691268155906517	27.526784461913195	22.344665032873905
58-59	22.424132182318914	27.75772475482563	27.50885702555993	22.309286037295525
60-61	22.483222428310402	27.684794379610395	27.532264124577686	22.299719067501517
62-63	22.459511753512466	27.743118757555074	27.470690858904046	22.326678630028418
64-65	22.929996604764444	27.706327674624287	27.055382557647206	22.308293162964063
66-67	22.493597449635057	27.72970258344249	27.310228813279906	22.466471153642548
68-69	22.485582925755867	27.716601094348903	27.412784204640207	22.385031775255023
70-71	22.443229405164438	27.556824629086798	27.43267356857619	22.567272397172573
72-73	22.547714125928675	27.57266593007948	27.331489834982982	22.548130109008863
74-75	22.370864229700498	27.715182605236087	27.350733388592996	22.563219776470415
76-77	22.48520826146101	27.664819390832594	27.292169898669965	22.557802449036426
78-79	22.466911288673867	27.695994512006077	27.274119687138498	22.562974512181558
80-81	22.460535557290974	27.68936956760199	27.280542990129387	22.569551884977646
82-83	22.49660082478427	27.66173138620221	27.376881561373494	22.464786227640023
84-85	22.62978811062358	27.625912781147637	27.294807464868708	22.449491643360073
86-87	22.410578944714533	27.68157480727318	27.41502218940784	22.492824058604455
88-89	22.481170664526957	27.671164807083777	27.351652459008385	22.496012069380885
90-91	22.511342817725723	27.711181317753663	27.33736503486953	22.44011082965108
92-93	22.487439936598104	27.65770756859307	27.405889645735336	22.44896284907349
94-95	22.379457937374696	27.759850583233085	27.411511812625623	22.449179666766593
96-97	22.467209295074632	27.833163252837927	27.479665479333466	22.21996197275398
98-99	23.561722052184315	29.764885391523865	27.674187611579754	18.999204944712066
100	22.455364733453706	30.801912977462347	21.562742562110934	25.179979726973016
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1762.0
1	1661.5
2	2255.5
3	3170.5
4	4208.5
5	6893.5
6	9456.5
7	11428.0
8	13044.0
9	13378.0
10	12684.5
11	11734.5
12	12576.0
13	16409.0
14	26069.5
15	40050.5
16	52125.5
17	60251.0
18	63244.0
19	61255.0
20	56153.5
21	49675.5
22	44707.0
23	44390.5
24	49918.0
25	61207.0
26	77490.0
27	96975.0
28	118545.0
29	143633.0
30	169754.0
31	195375.0
32	223270.0
33	251752.0
34	280066.0
35	307115.5
36	336307.5
37	364129.5
38	385920.5
39	405429.5
40	424611.5
41	440723.0
42	452690.5
43	465135.0
44	475670.0
45	485356.5
46	491544.5
47	492412.5
48	491353.0
49	483981.5
50	472526.5
51	457829.5
52	438530.0
53	416853.5
54	394382.5
55	367851.5
56	337909.5
57	306583.5
58	272335.5
59	235969.0
60	197391.0
61	160670.0
62	127543.5
63	98187.5
64	73679.5
65	55239.0
66	41904.0
67	32237.5
68	24717.5
69	17676.0
70	12357.5
71	8845.0
72	6132.5
73	4229.5
74	2877.0
75	1926.0
76	1306.0
77	861.0
78	584.5
79	412.5
80	288.5
81	199.0
82	138.5
83	100.5
84	73.5
85	48.5
86	35.0
87	30.5
88	21.0
89	12.0
90	6.5
91	3.0
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	0.0
3	0.0030429208730234805
4	0.011292425902721698
5	0.015024960571331238
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.001595152529778264
26-27	0.03517172102196298
28-29	0.08255969935358155
30-31	0.0792912992399465
32-33	0.03475227341755761
34-35	7.505164138590448E-4
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	1.242013636453678
56-57	2.3299948390614314E-5
58-59	0.0
60-61	0.0
62-63	0.0
64-65	5.26601553431564
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.008779443445677527
74-75	0.031074525865213705
76-77	0.004768623663540401
78-79	0.0
80-81	0.0
82-83	0.0033707985808989595
84-85	0.03693964926862828
86-87	0.03927209755836561
88-89	0.012354805812397236
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	163.0
20-21	604.0
22-23	1349.0
24-25	3613.0
26-27	6377.0
28-29	8845.0
30-31	20671.0
32-33	25926.0
34-35	36021.0
36-37	53279.0
38-39	63454.0
40-41	66861.0
42-43	79046.0
44-45	94290.0
46-47	92953.0
48-49	79634.0
50-51	71978.0
52-53	74942.0
54-55	72313.0
56-57	75106.0
58-59	77171.0
60-61	76537.0
62-63	80988.0
64-65	76263.0
66-67	80396.0
68-69	82497.0
70-71	82478.0
72-73	78001.0
74-75	79983.0
76-77	79272.0
78-79	84845.0
80-81	87446.0
82-83	85017.0
84-85	86588.0
86-87	93569.0
88-89	101221.0
90-91	109416.0
92-93	119136.0
94-95	149295.0
96-97	407216.0
98-99	739639.0
100-101	7816297.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.46903476011796
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.51936155906058	75.61614063235857
2	7.266264833067927	12.27548753521275
3	1.7502627743488954	4.43529021377452
4	0.6589548314895824	2.2264511426576488
5	0.31747208350684747	1.3408280228553486
6	0.15348162546445807	0.7778666853838029
7	0.09952347413955787	0.588465625757941
8	0.05971860226156032	0.4035498152205918
9	0.03851043281076736	0.29276451789479063
>10	0.13283047631745518	1.7379538149762432
>50	0.002702468803599294	0.1524527254836502
>100	8.473597605418054E-4	0.10314616283438854
>500	5.211205927521369E-5	0.02786266900560987
>1k	1.7366908897304353E-5	0.021740436584195055
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.620172444825725E-6
2	0.0	0.0	0.0	0.0	8.620172444825725E-6
3	0.0	0.0	0.0	0.0	8.620172444825725E-6
4	0.0	3.44806897793029E-5	0.0	8.620172444825725E-6	8.620172444825725E-6
5	0.0	5.172103466895435E-5	0.0	8.620172444825725E-6	8.620172444825725E-6
6	4.310086222412862E-5	5.172103466895435E-5	0.0	8.620172444825725E-6	1.724034488965145E-5
7	4.310086222412862E-5	5.172103466895435E-5	0.0	1.724034488965145E-5	1.724034488965145E-5
8	4.310086222412862E-5	6.034120711378007E-5	0.0	1.724034488965145E-5	1.724034488965145E-5
9	4.310086222412862E-5	1.6378327645168877E-4	0.0	1.724034488965145E-5	1.724034488965145E-5
10-11	5.172103466895434E-5	1.8102362134134021E-4	0.0	3.0170603556890038E-5	1.724034488965145E-5
12-13	6.034120711378007E-5	1.8102362134134021E-4	0.0	4.741094844654149E-5	1.724034488965145E-5
14-15	6.465129333619293E-5	2.1550431112064313E-4	0.0	6.0341207113780076E-5	1.724034488965145E-5
16-17	1.1206224178273442E-4	2.2412448356546883E-4	0.0	6.89613795586058E-5	2.5860517334477174E-5
18-19	1.1206224178273442E-4	2.49985000899946E-4	0.0	6.89613795586058E-5	4.310086222412862E-5
20-21	1.1206224178273442E-4	2.844656906792489E-4	0.0	8.189163822584439E-5	4.741094844654149E-5
22-23	1.2068241422756014E-4	2.973959493464875E-4	0.0	1.1637232800514727E-4	5.603112089136721E-5
24-25	1.2930258667238586E-4	3.189463804585518E-4	0.0	1.465429315620373E-4	7.758155200343152E-5
26-27	1.465429315620373E-4	3.2756655290337754E-4	0.0	2.284345697878817E-4	7.758155200343152E-5
28-29	1.5516310400686304E-4	3.2756655290337754E-4	0.0	7.327146578101865E-4	9.051181067067011E-5
30-31	1.594731902292759E-4	3.534270702378547E-4	0.0	0.0028662073379045533	1.0775215556032156E-4
32-33	1.6378327645168877E-4	3.534270702378547E-4	0.0	0.006378927609171036	1.2930258667238586E-4
34-35	1.7240344889651448E-4	4.0945819112922196E-4	0.0	0.01170188409385092	1.2930258667238586E-4
36-37	1.8102362134134021E-4	4.913498293550663E-4	0.0	0.020240164900450802	1.2930258667238586E-4
38-39	1.8102362134134021E-4	4.913498293550663E-4	0.0	0.030532650799572715	1.2930258667238586E-4
40-41	2.068841386758174E-4	5.258305191343692E-4	0.0	0.039161443416843265	1.2930258667238586E-4
42-43	2.155043111206431E-4	5.387607778016077E-4	0.0	0.048113492500794774	1.2930258667238586E-4
44-45	2.155043111206431E-4	6.034120711378007E-4	0.0	0.05567769382112935	1.2930258667238586E-4
46-47	2.155043111206431E-4	6.465129333619293E-4	0.0	0.06264710324277095	1.5085301778445018E-4
48-49	2.155043111206431E-4	6.551331058067551E-4	0.0	0.06902603085194199	1.7671353511892736E-4
50-51	2.155043111206431E-4	7.80125606256728E-4	0.0	0.07515928354643549	1.8102362134134021E-4
52-53	2.1981439734305595E-4	7.973659511463795E-4	0.0	0.08137442787915483	1.8102362134134021E-4
54-55	2.2412448356546883E-4	8.016760373687924E-4	0.0	0.08660687255316404	1.8102362134134021E-4
56-57	2.2412448356546883E-4	8.10296209813618E-4	0.0	0.09066266368845455	1.8102362134134021E-4
58-59	2.2412448356546883E-4	8.189163822584438E-4	0.0	0.09515377353220875	1.8102362134134021E-4
60-61	2.3274465601029457E-4	8.232264684808567E-4	0.0	0.09956299173773711	1.8964379378616592E-4
62-63	2.3274465601029457E-4	8.404668133705081E-4	0.0	0.10386445778770514	1.8964379378616592E-4
64-65	2.3274465601029457E-4	8.878777618170496E-4	0.0	0.10814006332033871	1.8964379378616592E-4
66-67	2.3274465601029457E-4	8.921878480394625E-4	0.0	0.1130277010965549	1.8964379378616592E-4
68-69	2.3274465601029457E-4	9.35288710263591E-4	0.0	0.11775155559631939	1.8964379378616592E-4
70-71	2.413648284551203E-4	9.611492275980682E-4	0.0	0.12188061819739092	2.068841386758174E-4
72-73	2.586051733447717E-4	9.740794862653068E-4	0.0	0.1251002526055333	2.4136482845512028E-4
74-75	2.586051733447717E-4	9.99940003599784E-4	0.0	0.12788025821898963	2.4136482845512028E-4
76-77	2.586051733447717E-4	0.0010473509520463255	0.0	0.13048786038354943	2.4567491467753313E-4
78-79	2.672253457895974E-4	0.0010602812107135642	0.0	0.13240153866630072	2.49985000899946E-4
80-81	2.672253457895974E-4	0.0010602812107135642	0.0	0.13304805159966265	2.542950871223589E-4
82-83	2.672253457895974E-4	0.0010602812107135642	0.0	0.1334747501356815	2.586051733447717E-4
84-85	2.672253457895974E-4	0.0010689013831583897	0.0	0.13366439392946766	2.586051733447717E-4
86-87	2.672253457895974E-4	0.0010861417280480412	0.0	0.13378938642991767	2.586051733447717E-4
88	2.758455182344232E-4	0.0011292425902721698	0.0	0.1338023166885849	2.586051733447717E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14280	0.0	49.60354	1
GTATCAA	25160	0.0	37.498238	1
TCAACGC	30040	0.0	30.92302	4
ATCAACG	30280	0.0	30.495386	3
CAACGCA	30970	0.0	29.990292	5
TATCAAC	31835	0.0	29.287302	2
AACGCAG	32020	0.0	29.000977	6
ACGCAGA	37275	0.0	24.923883	7
CGCAGAG	37950	0.0	24.469336	8
GTGGTAT	6675	0.0	22.693935	1
GCAGAGT	44610	0.0	20.797089	9
GTACATG	32475	0.0	20.73436	1
TGGTATC	6730	0.0	20.669754	2
TACATGG	32405	0.0	20.476282	2
GAGTACT	26200	0.0	20.236961	12-13
ACATGGG	32855	0.0	19.442545	3
CAGAGTA	42935	0.0	18.871365	10-11
GTACTTT	28925	0.0	18.360268	14-15
AGAGTAC	39885	0.0	18.218307	10-11
CATGGGG	25125	0.0	17.56054	4
>>END_MODULE