FastQCFastQC Report
Fri 27 May 2016
SRR2139903_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139903_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7037243
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97300.047.798081
GTATCAA169300.035.3050271
TCAACGC203800.028.5036184
ATCAACG204200.028.3665713
CAACGCA208750.027.8247595
TATCAAC214250.027.2514652
AACGCAG214600.027.1020936
GTGGTAT49050.024.8166121
TGGTATC47500.023.859082
ACGCAGA247700.023.4296327
CGCAGAG252400.023.0099728
TACATGG206650.020.6164632
GTACATG211500.020.5009251
GCAGAGT293500.019.74499
ACATGGG213150.019.4166853
GAGTACT178900.019.36315212-13
CAGAGTA285050.018.25450110-11
AGAGTAC264500.017.55476810-11
CATGGGG160250.017.5225054
GTACTTT197900.017.5148614-15
Produced by FastQC (version 0.11.5)