##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139903_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7037243
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.729232456517416	33.0	33.0	33.0	33.0	33.0
2	32.74418675040779	33.0	33.0	33.0	33.0	33.0
3	32.64736303691659	33.0	33.0	33.0	33.0	33.0
4	36.629779162095154	37.0	37.0	37.0	37.0	37.0
5	36.681736867690944	37.0	37.0	37.0	37.0	37.0
6	36.695299991772345	37.0	37.0	37.0	37.0	37.0
7	36.67966517569452	37.0	37.0	37.0	37.0	37.0
8	36.68295140582754	37.0	37.0	37.0	37.0	37.0
9	36.67918487396272	37.0	37.0	37.0	37.0	37.0
10-11	36.65297624367952	37.0	37.0	37.0	37.0	37.0
12-13	36.62792495583853	37.0	37.0	37.0	37.0	37.0
14-15	38.79003410852801	40.0	37.0	40.0	37.0	40.0
16-17	38.74916988087522	40.0	37.0	40.0	37.0	40.0
18-19	38.76560991285935	40.0	37.0	40.0	37.0	40.0
20-21	38.79012952948037	40.0	37.0	40.0	37.0	40.0
22-23	38.826239333636664	40.0	37.0	40.0	37.0	40.0
24-25	38.84673850224959	40.0	37.0	40.0	37.0	40.0
26-27	38.57006776629849	40.0	37.0	40.0	37.0	40.0
28-29	38.54203658009954	40.0	37.0	40.0	37.0	40.0
30-31	38.55263784205765	40.0	37.0	40.0	37.0	40.0
32-33	38.54868734271051	40.0	37.0	40.0	37.0	40.0
34-35	38.37762225185507	40.0	37.0	40.0	37.0	40.0
36-37	38.4399205202247	40.0	37.0	40.0	37.0	40.0
38-39	38.374272502088445	40.0	37.0	40.0	37.0	40.0
40-41	38.37792623088832	40.0	37.0	40.0	37.0	40.0
42-43	38.33386557335244	40.0	37.0	40.0	37.0	40.0
44-45	38.25820624814516	40.0	37.0	40.0	37.0	40.0
46-47	38.18026908193351	40.0	37.0	40.0	37.0	40.0
48-49	37.95071383076998	40.0	37.0	40.0	33.0	40.0
50-51	38.043796293929006	40.0	37.0	40.0	33.0	40.0
52-53	38.006597324033294	40.0	37.0	40.0	33.0	40.0
54-55	33.457313120756	38.5	33.0	40.0	10.5	40.0
56-57	36.66887601945819	37.0	35.0	40.0	30.0	40.0
58-59	37.45937075139876	38.5	37.0	40.0	33.0	40.0
60-61	37.49749039402591	38.5	37.0	40.0	33.0	40.0
62-63	37.26470011257764	37.0	37.0	40.0	33.0	40.0
64-65	32.37310698300293	37.0	33.0	40.0	8.5	40.0
66-67	35.4357597137782	37.0	33.0	40.0	30.0	40.0
68-69	36.29653830840003	37.0	37.0	40.0	33.0	40.0
70-71	36.21821292620639	37.0	37.0	38.5	33.0	40.0
72-73	35.58227507272373	37.0	37.0	37.0	33.0	40.0
74-75	35.63765431555251	37.0	35.0	37.0	33.0	40.0
76-77	33.82582123243247	35.0	33.0	37.0	30.0	37.0
78-79	35.34572884135646	37.0	33.0	37.0	33.0	37.0
80-81	35.54871877836375	37.0	33.0	37.0	33.0	37.0
82-83	35.48567067113844	37.0	33.0	37.0	33.0	37.0
84-85	35.36941694063735	37.0	33.0	37.0	33.0	37.0
86-87	35.26753108091782	37.0	33.0	37.0	33.0	37.0
88-89	35.18362166400449	37.0	33.0	37.0	33.0	37.0
90-91	35.088787153853886	37.0	33.0	37.0	33.0	37.0
92-93	35.02481384702865	37.0	33.0	37.0	33.0	37.0
94-95	34.975429149827505	37.0	33.0	37.0	33.0	37.0
96-97	34.943574579489805	37.0	33.0	37.0	33.0	37.0
98-99	34.90768290888492	37.0	33.0	37.0	33.0	37.0
100	34.862377081046844	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	35.0
21	159.0
22	591.0
23	1740.0
24	4200.0
25	8826.0
26	16113.0
27	26470.0
28	40806.0
29	59053.0
30	82493.0
31	114719.0
32	157655.0
33	219514.0
34	323698.0
35	539722.0
36	1142098.0
37	1809753.0
38	2396280.0
39	93314.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.30386814836435	19.928173575930234	14.134327889487402	23.633630386218012
2	14.766422020669173	20.635837074263318	41.60707538449361	22.990665520573895
3	18.84585553377551	25.253566068146437	30.057601070452815	25.84297732762524
4	12.527247636927457	17.444911382114977	38.63040564835093	31.397435332606634
5	13.390427575785743	37.67542068132766	35.41090884903908	13.523242893847517
6	30.677184232518336	37.506975387946675	18.322743722221897	13.493096657313098
7	27.122866156533178	32.547149501587484	22.413237684132834	17.916746657746508
8	24.737159140305373	36.86455050649807	20.650601378977534	17.747688974219024
9	25.417823428862697	16.992577917232644	21.105793845686442	36.48380480821822
10-11	24.525975584472498	26.876910460531207	28.372091172636782	20.22502278235951
12-13	25.191641101493868	24.221026615110492	28.794735381455496	21.792596901940147
14-15	22.1082389793844	25.79838439570724	26.558156368907536	25.535220256000823
16-17	21.65239284759671	29.00098092392148	27.220667241418266	22.125958987063544
18-19	21.59290222037238	28.02758125589808	29.21047347661577	21.169043047113764
20-21	22.404967064789076	27.146050130071824	29.246376352328845	21.20260645281025
22-23	22.13800394802915	27.08487836336918	29.085979593630544	21.691138094971127
24-25	21.869201759903902	27.14348367983861	29.216182945630877	21.771131614626615
26-27	21.86696204325914	27.390934850841482	28.9914335050966	21.750669600802773
28-29	21.726089229322028	27.520296382072797	29.083872156537854	21.669742232067325
30-31	22.077905876978953	27.327078196716307	28.9391834999434	21.655832426361336
32-33	21.805377428283343	27.454776302296818	28.92560691016387	21.814239359255968
34-35	21.976807582800507	27.549145686453226	28.86017457668033	21.613872154065938
36-37	21.952886727917356	27.459245101026692	28.841817358272685	21.746050812783267
38-39	21.81052567026401	27.59019680670733	28.886155783807677	21.713121739220988
40-41	22.06042630976195	27.45119812242891	28.722397398242894	21.765978169566246
42-43	21.943105425303198	27.57293692417277	28.512047798185048	21.971909852338985
44-45	22.114808795226406	27.703296370637215	28.275726086089907	21.906168748046476
46-47	22.12891239329055	27.702115859802586	27.998898897985637	22.170072848921237
48-49	22.093363663815037	27.88597156811904	27.89060150336188	22.13006326470405
50-51	22.086160115784683	27.98196151908579	27.830289739042556	22.10158862608697
52-53	22.24062957604926	27.871820503849786	27.719672446562114	22.16787747353884
54-55	23.182155661426336	27.72898544074106	27.11508917501978	21.97376972281283
56-57	22.196447100013653	27.95340513326499	27.700456750544344	22.149691016177012
58-59	22.17208048493514	27.982887664462808	27.700933969644037	22.144097880958018
60-61	22.26831795997363	27.929129367062455	27.699958308679424	22.102594364284496
62-63	22.228776744778607	27.964328956484707	27.654605703349212	22.15228859538748
64-65	22.689950978297908	27.9400375344302	27.25139079384534	22.118620693426553
66-67	22.26613097964452	27.98544116751769	27.487093704506204	22.26133414833159
68-69	22.240865639903404	27.98953515094278	27.57133727188597	22.198261937267844
70-71	22.187434105522843	27.778944604883172	27.625056778640396	22.408564510953592
72-73	22.33201383083047	27.806547819698235	27.53621688018838	22.325221469282912
74-75	22.110726528916764	27.95528338969494	27.552000391597947	22.38198968979035
76-77	22.255682536212678	27.89258103878618	27.484943073187374	22.36679335181377
78-79	22.23424904023537	27.959861780373686	27.469872459783655	22.336016719607294
80-81	22.1876709242462	27.964835988057335	27.507376569261965	22.340116518434503
82-83	22.26024561765486	27.887853761106125	27.59980181983936	22.252098801399658
84-85	22.414759569412897	27.85905249281946	27.478859413601054	22.24732852416659
86-87	22.181241792978998	27.892405332493514	27.5965498611311	22.329803013396393
88-89	22.32222930674386	27.845208877143595	27.532867512305927	22.29969430380662
90-91	22.326866078579396	27.89014965253573	27.585463903213782	22.19752036567109
92-93	22.313640357309374	27.878680732157115	27.60383170008125	22.203847210452263
94-95	22.173764042659577	27.958750843542735	27.602413253526358	22.26507186027133
96-97	22.28107912111879	27.99949349619399	27.667760692877007	22.051666689810215
98-99	23.341036204406333	29.973586096137012	27.87152651058888	18.81385118886778
100	22.2312707713267	31.028731826364	21.693628182253804	25.046369220055496
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1167.0
1	1078.0
2	1554.5
3	2329.0
4	3235.0
5	5362.5
6	7203.0
7	8482.5
8	9522.0
9	9598.0
10	8977.0
11	8228.0
12	8830.5
13	11716.0
14	18883.0
15	29050.0
16	37871.5
17	43806.5
18	45598.5
19	43567.5
20	39814.0
21	35429.5
22	32118.5
23	32062.5
24	35977.0
25	43843.0
26	54806.5
27	67778.5
28	81757.0
29	97315.0
30	113482.5
31	129541.0
32	147223.0
33	164306.0
34	181266.5
35	198132.5
36	215088.5
37	231718.0
38	243968.5
39	252640.0
40	262259.0
41	270679.0
42	275975.5
43	283216.0
44	289551.5
45	294244.5
46	297498.0
47	298266.5
48	297229.0
49	291655.5
50	284229.5
51	275533.5
52	263482.5
53	249484.5
54	235477.0
55	218880.5
56	200396.5
57	181577.5
58	160764.0
59	139673.0
60	117926.0
61	96071.5
62	76060.5
63	58991.0
64	44904.0
65	33860.0
66	25950.5
67	20301.0
68	15747.0
69	11459.5
70	8033.5
71	5805.5
72	4098.5
73	2736.5
74	1834.5
75	1294.0
76	864.0
77	541.5
78	372.5
79	264.5
80	185.5
81	137.5
82	98.0
83	63.5
84	36.0
85	22.0
86	16.5
87	13.0
88	9.0
89	6.5
90	5.5
91	3.5
92	1.5
93	1.0
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	0.0
3	0.0031404344002331597
4	0.012121224178275498
5	0.01644109774239713
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0017483030622238826
26-27	0.03554151432303831
28-29	0.0828427093329266
30-31	0.08079228434040682
32-33	0.0355992883568769
34-35	9.091557930082769E-4
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	1.2555669512421164
56-57	6.248720964927492E-5
58-59	0.0
60-61	0.0
62-63	0.0
64-65	5.237474158371813
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.009358611121514244
74-75	0.03199405450899958
76-77	0.0049671814290898385
78-79	0.0
80-81	0.0
82-83	0.0038607334277796237
84-85	0.03863814757374266
86-87	0.041837730214937384
88-89	0.01410298215382464
90-91	9.267442926221053E-6
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	111.0
20-21	309.0
22-23	801.0
24-25	2308.0
26-27	3996.0
28-29	6471.0
30-31	14591.0
32-33	18619.0
34-35	25772.0
36-37	38550.0
38-39	47177.0
40-41	48505.0
42-43	57872.0
44-45	68632.0
46-47	68311.0
48-49	59261.0
50-51	53222.0
52-53	54391.0
54-55	53277.0
56-57	55956.0
58-59	57131.0
60-61	55872.0
62-63	57634.0
64-65	55277.0
66-67	58852.0
68-69	60236.0
70-71	59186.0
72-73	55549.0
74-75	56968.0
76-77	57712.0
78-79	61166.0
80-81	61972.0
82-83	59124.0
84-85	59672.0
86-87	62783.0
88-89	67430.0
90-91	69347.0
92-93	72219.0
94-95	86540.0
96-97	229205.0
98-99	420439.0
100-101	4534797.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.67968198966196
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.8562888974258	76.98858257502634
2	7.195307176304784	12.32983261367453
3	1.7401417131758696	4.472843658055622
4	0.5719678342617833	1.960240885914611
5	0.25873629625219163	1.1084221791035374
6	0.12853781900522562	0.6607847673607475
7	0.07854278659436738	0.4710664684591097
8	0.04761326808187572	0.32635917341949067
9	0.030857050421564714	0.23794400405327665
>10	0.089613446238536	1.162169206645048
>50	0.0016261183964520897	0.0932085935048081
>100	6.409152008234241E-4	0.10112967748278935
>500	1.013430618729878E-4	0.05607064905774085
>1k	2.5335578840879505E-5	0.03134554824231654
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.421011040829484E-5	0.0
2	0.0	0.0	0.0	1.421011040829484E-5	0.0
3	0.0	0.0	0.0	1.421011040829484E-5	0.0
4	0.0	2.842022081658968E-5	0.0	1.421011040829484E-5	0.0
5	2.842022081658968E-5	2.842022081658968E-5	0.0	1.421011040829484E-5	0.0
6	2.842022081658968E-5	2.842022081658968E-5	0.0	1.421011040829484E-5	0.0
7	2.842022081658968E-5	2.842022081658968E-5	0.0	1.421011040829484E-5	0.0
8	2.842022081658968E-5	4.263033122488452E-5	0.0	1.421011040829484E-5	2.842022081658968E-5
9	2.842022081658968E-5	8.526066244976904E-5	0.0	2.842022081658968E-5	7.10505520414742E-5
10-11	2.842022081658968E-5	8.526066244976904E-5	0.0	7.10505520414742E-5	7.10505520414742E-5
12-13	4.263033122488452E-5	8.526066244976904E-5	0.0	8.526066244976904E-5	7.10505520414742E-5
14-15	4.973538642903194E-5	9.947077285806388E-5	0.0	9.947077285806388E-5	7.10505520414742E-5
16-17	6.394549683732677E-5	9.947077285806388E-5	0.0	9.947077285806388E-5	9.947077285806388E-5
18-19	7.10505520414742E-5	1.2078593847050613E-4	0.0	9.947077285806388E-5	1.492061592870958E-4
20-21	7.10505520414742E-5	1.2789099367465354E-4	0.0	9.947077285806388E-5	1.7052132489953808E-4
22-23	8.526066244976904E-5	1.9183649051198034E-4	0.0	1.1368088326635872E-4	2.1315165612442258E-4
24-25	1.0657582806221129E-4	2.557819873493071E-4	0.0	1.6341626969539064E-4	2.557819873493071E-4
26-27	1.3499604887880098E-4	2.6999209775760197E-4	0.0	3.339375945949287E-4	2.557819873493071E-4
28-29	1.421011040829484E-4	2.842022081658968E-4	0.0	0.0010586532254179657	2.6999209775760197E-4
30-31	1.492061592870958E-4	2.842022081658968E-4	0.0	0.003957515748710113	2.842022081658968E-4
32-33	1.5631121449124322E-4	2.842022081658968E-4	0.0	0.008185023595177827	3.1262242898248645E-4
34-35	1.6341626969539064E-4	3.481477050032236E-4	0.0	0.014409051954010967	3.694628706156658E-4
36-37	1.776263801036855E-4	4.1209320184055034E-4	0.0	0.024576385951145925	3.694628706156658E-4
38-39	1.847314353078329E-4	4.2630331224884516E-4	0.0	0.037045757834424646	3.836729810239607E-4
40-41	2.1315165612442258E-4	4.831437538820245E-4	0.0	0.0474830839293172	3.978830914322555E-4
42-43	2.1315165612442258E-4	4.90248809086172E-4	0.0	0.05765752298165631	4.191982570446978E-4
44-45	2.1315165612442258E-4	5.470892507193513E-4	0.0	0.06603438306734613	4.2630331224884516E-4
46-47	2.1315165612442258E-4	5.968246371483832E-4	0.0	0.07382152357109169	4.7603869867787713E-4
48-49	2.1315165612442258E-4	5.968246371483832E-4	0.0	0.08189286628300316	4.973538642903194E-4
50-51	2.2736176653271743E-4	6.394549683732678E-4	0.0	0.0893176489713372	4.973538642903194E-4
52-53	2.4157187694101226E-4	6.394549683732678E-4	0.0	0.09605324130486897	5.25774085106909E-4
54-55	2.4157187694101226E-4	6.394549683732678E-4	0.0	0.10219200900125233	5.541943059234987E-4
56-57	2.4157187694101226E-4	6.394549683732678E-4	0.0	0.10709449709211405	5.541943059234987E-4
58-59	2.4867693214515965E-4	6.465600235774152E-4	0.0	0.11263644015134905	5.612993611276461E-4
60-61	2.557819873493071E-4	6.536650787815627E-4	0.0	0.11804338716170523	6.039296923525307E-4
62-63	2.557819873493071E-4	6.536650787815627E-4	0.0	0.12299561063899599	6.252448579649729E-4
64-65	2.557819873493071E-4	7.673459620479214E-4	0.0	0.12791941389547012	6.323499131691203E-4
66-67	2.557819873493071E-4	7.673459620479214E-4	0.0	0.13344714684429684	6.536650787815625E-4
68-69	2.6999209775760197E-4	7.957661828645109E-4	0.0	0.13954328420945533	6.820852995981523E-4
70-71	2.6999209775760197E-4	8.099762932728058E-4	0.0	0.14476549978450368	7.46030796435479E-4
72-73	2.6999209775760197E-4	8.099762932728058E-4	0.0	0.14912089862464606	7.886611276603636E-4
74-75	2.6999209775760197E-4	8.241864036811007E-4	0.0	0.1526307958954949	8.028712380686585E-4
76-77	2.6999209775760197E-4	8.455015692935429E-4	0.0	0.1561122729455271	8.099762932728058E-4
78-79	2.842022081658968E-4	8.526066244976903E-4	0.0	0.15809458334748422	8.099762932728058E-4
80-81	2.842022081658968E-4	8.526066244976903E-4	0.0	0.15876956359187824	8.31291458885248E-4
82-83	2.842022081658968E-4	8.526066244976903E-4	0.0	0.1593805983394349	8.881319005184275E-4
84-85	2.842022081658968E-4	8.668167349059852E-4	0.0	0.1595440146091303	9.094470661308697E-4
86-87	2.842022081658968E-4	8.668167349059852E-4	0.0	0.15967190560280498	9.30762231743312E-4
88	2.842022081658968E-4	9.236571765391645E-4	0.0	0.1596790106580091	9.378672869474594E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9730	0.0	47.79808	1
GTATCAA	16930	0.0	35.305027	1
TCAACGC	20380	0.0	28.503618	4
ATCAACG	20420	0.0	28.366571	3
CAACGCA	20875	0.0	27.824759	5
TATCAAC	21425	0.0	27.251465	2
AACGCAG	21460	0.0	27.102093	6
GTGGTAT	4905	0.0	24.816612	1
TGGTATC	4750	0.0	23.85908	2
ACGCAGA	24770	0.0	23.429632	7
CGCAGAG	25240	0.0	23.009972	8
TACATGG	20665	0.0	20.616463	2
GTACATG	21150	0.0	20.500925	1
GCAGAGT	29350	0.0	19.7449	9
ACATGGG	21315	0.0	19.416685	3
GAGTACT	17890	0.0	19.363152	12-13
CAGAGTA	28505	0.0	18.254501	10-11
AGAGTAC	26450	0.0	17.554768	10-11
CATGGGG	16025	0.0	17.522505	4
GTACTTT	19790	0.0	17.51486	14-15
>>END_MODULE