FastQCFastQC Report
Fri 27 May 2016
SRR2139902_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139902_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12932744
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA182150.050.953071
GTATCAA332200.038.0479661
TCAACGC405300.030.7263014
ATCAACG405900.030.4942553
TATCAAC417100.029.9995212
CAACGCA414850.029.9537035
AACGCAG428650.029.0344836
ACGCAGA492650.025.2197637
CGCAGAG497450.024.9594338
GTGGTAT83850.024.737051
TGGTATC84600.023.2693922
GCAGAGT573000.021.6095719
GAGTACT354300.020.18602812-13
CAGAGTA556700.019.38235310-11
GTACATG395450.019.2714521
TACATGG393300.018.8827132
AGAGTAC521600.018.71514110-11
GTACTTT392950.018.20094514-15
ACATGGG396550.018.163353
AGTACTT381150.017.16296412-13
Produced by FastQC (version 0.11.5)