##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139902_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12932744
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.718413895767206	33.0	33.0	33.0	33.0	33.0
2	32.732008922468424	33.0	33.0	33.0	33.0	33.0
3	32.634958056851666	33.0	33.0	33.0	33.0	33.0
4	36.61599116165912	37.0	37.0	37.0	37.0	37.0
5	36.67021708618063	37.0	37.0	37.0	37.0	37.0
6	36.6807959702906	37.0	37.0	37.0	37.0	37.0
7	36.66403664991745	37.0	37.0	37.0	37.0	37.0
8	36.666159169314724	37.0	37.0	37.0	37.0	37.0
9	36.66312647957773	37.0	37.0	37.0	37.0	37.0
10-11	36.63394384826607	37.0	37.0	37.0	37.0	37.0
12-13	36.60623151591031	37.0	37.0	37.0	37.0	37.0
14-15	38.758332454427304	40.0	37.0	40.0	37.0	40.0
16-17	38.71616963113165	40.0	37.0	40.0	37.0	40.0
18-19	38.73410553862351	40.0	37.0	40.0	37.0	40.0
20-21	38.7588837168591	40.0	37.0	40.0	37.0	40.0
22-23	38.79563336290812	40.0	37.0	40.0	37.0	40.0
24-25	38.81766895682999	40.0	37.0	40.0	37.0	40.0
26-27	38.54039907617691	40.0	37.0	40.0	37.0	40.0
28-29	38.50947389792904	40.0	37.0	40.0	37.0	40.0
30-31	38.51607797556535	40.0	37.0	40.0	37.0	40.0
32-33	38.50727826731026	40.0	37.0	40.0	37.0	40.0
34-35	38.3360307886407	40.0	37.0	40.0	37.0	40.0
36-37	38.394870803442686	40.0	37.0	40.0	37.0	40.0
38-39	38.327155885386	40.0	37.0	40.0	37.0	40.0
40-41	38.325312189915536	40.0	37.0	40.0	37.0	40.0
42-43	38.27310079968019	40.0	37.0	40.0	37.0	40.0
44-45	38.19235831525356	40.0	37.0	40.0	37.0	40.0
46-47	38.10973777991781	40.0	37.0	40.0	37.0	40.0
48-49	37.87590351316041	40.0	37.0	40.0	33.0	40.0
50-51	37.96712465556811	40.0	37.0	40.0	33.0	40.0
52-53	37.927089313140094	40.0	37.0	40.0	33.0	40.0
54-55	33.429287562620736	37.0	33.0	40.0	10.5	40.0
56-57	36.59704148334626	37.0	35.0	40.0	30.0	40.0
58-59	37.37942974770625	38.5	37.0	40.0	33.0	40.0
60-61	37.41421142995473	38.5	37.0	40.0	33.0	40.0
62-63	37.17788182798746	37.0	37.0	40.0	33.0	40.0
64-65	32.33781057278017	37.0	33.0	40.0	8.5	40.0
66-67	35.35889616757775	37.0	33.0	40.0	30.0	40.0
68-69	36.21236981354781	37.0	37.0	40.0	33.0	40.0
70-71	36.14079078760846	37.0	37.0	38.5	33.0	40.0
72-73	35.51166936003435	37.0	35.0	37.0	33.0	40.0
74-75	35.56253129611386	37.0	33.0	37.0	33.0	40.0
76-77	33.74212132194506	35.0	33.0	37.0	27.0	37.0
78-79	35.27687448163195	37.0	33.0	37.0	33.0	37.0
80-81	35.48875100795603	37.0	33.0	37.0	33.0	37.0
82-83	35.42599349447243	37.0	33.0	37.0	33.0	37.0
84-85	35.310821029963634	37.0	33.0	37.0	33.0	37.0
86-87	35.20955157255845	37.0	33.0	37.0	33.0	37.0
88-89	35.12600167393854	37.0	33.0	37.0	33.0	37.0
90-91	35.031698248767455	37.0	33.0	37.0	33.0	37.0
92-93	34.96402253809913	37.0	33.0	37.0	33.0	37.0
94-95	34.913854394328816	37.0	33.0	37.0	33.0	37.0
96-97	34.88257720339525	37.0	33.0	37.0	33.0	37.0
98-99	34.853865668046865	37.0	33.0	37.0	33.0	37.0
100	34.81072711293841	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	4.0
19	8.0
20	77.0
21	320.0
22	1237.0
23	3784.0
24	9165.0
25	18891.0
26	34402.0
27	55799.0
28	83954.0
29	120351.0
30	165161.0
31	225050.0
32	301967.0
33	417468.0
34	606329.0
35	1000850.0
36	2088716.0
37	3321974.0
38	4316886.0
39	160350.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.4857632688005	19.77100142088949	14.29682672138256	23.446408588927454
2	14.815316842272608	20.385178891656714	41.89947624417525	22.90002802189543
3	18.8999148182263	25.17709086612605	29.95375007036621	25.96924424528144
4	12.458000648356775	17.45448510697268	38.55021578897224	31.537298455698306
5	13.440298987639213	37.605471004831344	35.31436515395147	13.63986485357797
6	30.788013742481873	37.419924186236116	18.21766517608328	13.57439689519873
7	27.27687952378861	32.48145946444158	22.205032435498605	18.03662857627121
8	24.77715479406381	36.995404842158784	20.3544661519628	17.8729742118146
9	25.437787989926964	16.998295180048412	20.90518454552259	36.65873228450204
10-11	24.66259673894419	26.821894873972607	28.229465456054804	20.286042931028405
12-13	25.356819094230893	24.157282476170565	28.649384075026923	21.83651435457162
14-15	22.229590255555976	25.77077223518845	26.48915033035526	25.510487178900316
16-17	21.724299189715655	28.94884875166477	27.129385689533482	22.197466369086097
18-19	21.619507043516826	27.897583065125236	29.217913847208294	21.26499604414964
20-21	22.546553401286378	27.001974102374955	29.220275956778526	21.231196539560145
22-23	22.238686046352925	26.915740014311325	29.097491636746376	21.74808230258938
24-25	21.927140930394945	27.034760375340834	29.225790304195243	21.812308390068978
26-27	21.967948110408187	27.240263242107993	28.989708777398203	21.802079870085617
28-29	21.785457892496616	27.358959143874284	29.116276680466953	21.739306283162147
30-31	22.134081125936326	27.15403338818942	28.96598284137248	21.745902644501776
32-33	21.86787965553832	27.343210308696364	28.93241885295629	21.85649118280903
34-35	22.01166276096432	27.40078454553368	28.894071403889903	21.693481289612098
36-37	22.018714888481576	27.328421942886493	28.816098910488535	21.836764258143397
38-39	21.88941141602347	27.479853564205875	28.86708274359585	21.763652276174806
40-41	22.13515401005469	27.34792492386052	28.72010057696176	21.79682048912303
42-43	22.021793156184284	27.45231552284458	28.526695819792263	21.99919550117887
44-45	22.203124475302904	27.5797543602178	28.247892093436374	21.96922907104292
46-47	22.1881053206762	27.636897042292887	27.935057090550192	22.239940546480717
48-49	22.16968893457808	27.773202306692625	27.84555262572526	22.21155613300403
50-51	22.15111824944699	27.89623768181166	27.761269458531302	22.191374610210048
52-53	22.29088726012923	27.774324649769135	27.647815499821178	22.28697259028046
54-55	23.25162210060197	27.60826156368227	27.085699681661364	22.0544166540544
56-57	22.272574683110548	27.828563328138372	27.670834254968856	22.228027733782223
58-59	22.22394684886221	27.924890938544234	27.656239637420626	22.19492257517293
60-61	22.299040370646754	27.864144982710766	27.65135502729692	22.18545961934556
62-63	22.252596079114788	27.904213690903035	27.578636799590896	22.26455343039128
64-65	22.743922314292845	27.857637272005864	27.21839986338852	22.18004055031277
66-67	22.330363391123072	27.919074841512952	27.42012349301359	22.330438274350385
68-69	22.277340257999516	27.92295790381004	27.502056001000835	22.29764583718961
70-71	22.232202204396405	27.70706343666898	27.558251810335125	22.502482548599488
72-73	22.33007111384684	27.78112928873886	27.490189870653005	22.398609726761297
74-75	22.188820801201153	27.910291951954928	27.46382329705958	22.437063949784335
76-77	22.266206082266233	27.86992613742764	27.42837228468612	22.435495495620003
78-79	22.259512291822293	27.884994282592245	27.372454572689552	22.483038852895906
80-81	22.241119602073667	27.8775329600231	27.433917398361768	22.447430039541462
82-83	22.301644126352205	27.870824604710755	27.51710082969726	22.310430439239777
84-85	22.46982126718829	27.807442323358927	27.420254150309127	22.30248225914366
86-87	22.230883625135707	27.843221190576905	27.53412422647763	22.39177095780976
88-89	22.321891405382075	27.827956127355986	27.498588718644505	22.351563748617433
90-91	22.360246266876462	27.85946818517232	27.494191270899744	22.286094277051472
92-93	22.35919641045758	27.8107646528441	27.547207249082494	22.282831687615825
94-95	22.224034048074955	27.891224627807198	27.581595202397892	22.303146121719962
96-97	22.311681837258288	27.99988976681908	27.628493912279517	22.05993448364312
98-99	23.388836538528853	29.920647433159946	27.8071814114995	18.883334616811702
100	22.333698245021186	30.918192901000985	21.693706505401515	25.054402348576314
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2090.0
1	1959.5
2	2878.5
3	4260.0
4	5878.5
5	9825.5
6	13349.0
7	15860.0
8	17819.5
9	18081.5
10	17003.0
11	15675.5
12	16910.0
13	22394.0
14	36088.0
15	55609.0
16	72275.0
17	83063.0
18	86734.0
19	83474.0
20	76280.5
21	67450.5
22	60544.5
23	60461.0
24	67975.5
25	81536.5
26	101112.5
27	124693.0
28	148926.5
29	175697.5
30	203273.0
31	231410.5
32	263809.0
33	294923.5
34	324413.0
35	353629.5
36	385749.0
37	416611.5
38	436884.5
39	451674.0
40	467420.0
41	481764.0
42	492527.5
43	505540.0
44	516310.5
45	524781.5
46	531069.0
47	532124.0
48	530161.0
49	521252.5
50	509963.0
51	495302.0
52	474707.0
53	454950.5
54	433868.5
55	405368.5
56	374531.0
57	341954.0
58	306604.0
59	270016.5
60	228152.5
61	186828.0
62	149055.0
63	115535.5
64	88167.0
65	66771.5
66	51573.5
67	40344.5
68	31049.5
69	22563.5
70	16000.0
71	11572.0
72	8358.5
73	5985.5
74	4193.5
75	2884.5
76	1943.0
77	1304.0
78	880.0
79	592.0
80	404.0
81	275.5
82	192.0
83	131.5
84	89.0
85	62.0
86	42.5
87	33.0
88	25.0
89	15.5
90	10.0
91	5.5
92	3.5
93	2.5
94	2.0
95	1.5
96	1.0
97	1.0
98	1.5
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	0.0
3	0.003092924440474504
4	0.012309839273088525
5	0.0168255089561813
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0017440753162103295
26-27	0.03581176928632254
28-29	0.08359800680861
30-31	0.08128722507919953
32-33	0.035981924470341174
34-35	9.226289943363426E-4
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	1.2383411553364496
56-57	4.647787298324058E-5
58-59	0.0
60-61	0.0
62-63	0.0
64-65	5.206288935065212
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.008817214570401815
74-75	0.030644223577552393
76-77	0.004652616532497029
78-79	0.0
80-81	0.0
82-83	0.003944913081995588
84-85	0.039137406767396216
86-87	0.042402249398233754
88-89	0.014059517254722145
90-91	9.888249432278518E-6
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	169.0
20-21	587.0
22-23	1391.0
24-25	4070.0
26-27	7175.0
28-29	10825.0
30-31	24287.0
32-33	30956.0
34-35	43698.0
36-37	65827.0
38-39	80933.0
40-41	84881.0
42-43	100730.0
44-45	121106.0
46-47	119508.0
48-49	103115.0
50-51	92379.0
52-53	93729.0
54-55	90476.0
56-57	94614.0
58-59	96770.0
60-61	96436.0
62-63	99026.0
64-65	94231.0
66-67	99921.0
68-69	102705.0
70-71	102786.0
72-73	95312.0
74-75	97282.0
76-77	97320.0
78-79	103449.0
80-81	106302.0
82-83	101609.0
84-85	101627.0
86-87	107626.0
88-89	116270.0
90-91	123310.0
92-93	129182.0
94-95	155743.0
96-97	422605.0
98-99	790166.0
100-101	8522610.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.29708635007549
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.7851051389103	71.36673272728446
2	7.96335944691754	12.94795841185489
3	2.241462275605527	5.466730565110177
4	0.8670991275569365	2.819705305882856
5	0.4289334302782103	1.743551905988087
6	0.2264777559810961	1.1047189010619898
7	0.1476960926540098	0.8405083398643249
8	0.08672676537006027	0.5640506666522022
9	0.06573487948277494	0.4809648756170664
>10	0.18284108829830112	2.2499906760432316
>50	0.0034626004522492477	0.1869703041416681
>100	0.0010302191228901195	0.1285323969634399
>500	3.559169849312475E-5	0.021946599092815555
>1k	3.558767153396739E-5	0.07763832444282394
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	7.73231110118626E-6	0.0	0.0	0.0
3	0.0	7.73231110118626E-6	0.0	0.0	0.0
4	0.0	3.86615555059313E-5	0.0	1.546462220237252E-5	0.0
5	0.0	3.86615555059313E-5	0.0	2.319693330355878E-5	0.0
6	0.0	3.86615555059313E-5	0.0	2.319693330355878E-5	7.73231110118626E-6
7	7.73231110118626E-6	4.639386660711756E-5	0.0	9.278773321423513E-5	7.73231110118626E-6
8	7.73231110118626E-6	5.412617770830382E-5	0.0	9.278773321423513E-5	7.73231110118626E-6
9	7.73231110118626E-6	1.546462220237252E-4	0.0	1.3918159982135268E-4	7.73231110118626E-6
10-11	6.572464436008322E-5	1.546462220237252E-4	0.0	1.3918159982135268E-4	7.73231110118626E-6
12-13	7.73231110118626E-5	1.546462220237252E-4	0.0	1.8944162197906338E-4	7.73231110118626E-6
14-15	7.73231110118626E-5	2.3583548858618095E-4	0.0	2.049062441814359E-4	1.1598466651779389E-5
16-17	8.892157766364199E-5	2.474339552379603E-4	0.0	2.3970164413677407E-4	1.546462220237252E-5
18-19	1.0052004431542139E-4	2.551662663391466E-4	0.0	3.0156013294626417E-4	1.546462220237252E-5
20-21	1.0052004431542139E-4	2.706308885415191E-4	0.0	3.440878440027886E-4	1.546462220237252E-5
22-23	1.1985082206838704E-4	2.8996166629448474E-4	0.0	3.7888324395812676E-4	1.546462220237252E-5
24-25	1.662446886755046E-4	3.324893773510092E-4	0.0	4.6007251052058247E-4	1.546462220237252E-5
26-27	1.7011084422609773E-4	3.479539995533817E-4	0.0	5.567263992854107E-4	1.546462220237252E-5
28-29	1.7011084422609773E-4	3.479539995533817E-4	0.0	0.0012217051539874292	1.546462220237252E-5
30-31	1.817093108778771E-4	3.7888324395812676E-4	0.0	0.003425413817825513	1.546462220237252E-5
32-33	1.8557546642847025E-4	3.86615555059313E-4	0.0	0.0069668123021688205	3.86615555059313E-5
34-35	2.049062441814359E-4	4.4847404386880304E-4	0.0	0.012193854606570732	4.639386660711756E-5
36-37	2.319693330355878E-4	5.103325326782931E-4	0.0	0.021066681595181963	5.0260022157710694E-5
38-39	2.3583548858618095E-4	5.296633104312589E-4	0.0	0.031629018559402394	6.185848880949008E-5
40-41	2.590324218897397E-4	5.721910214877832E-4	0.0	0.040992847302938965	6.185848880949008E-5
42-43	2.6289857744033287E-4	5.915217992407489E-4	0.0	0.05040306991308263	6.572464436008322E-5
44-45	2.7836319964270537E-4	6.417818213984596E-4	0.0	0.05818177488087602	7.345695546126947E-5
46-47	2.9382782184507786E-4	6.727110658032047E-4	0.0	0.06550040733814881	8.118926656245574E-5
48-49	2.9382782184507786E-4	6.727110658032047E-4	0.0	0.07256773968463305	8.505542211304887E-5
50-51	2.9769397739567104E-4	7.848295767704054E-4	0.0	0.0794610950313406	8.505542211304887E-5
52-53	3.0156013294626417E-4	7.886957323209985E-4	0.0	0.08617660722272087	9.665388876482825E-5
54-55	3.0156013294626417E-4	7.886957323209985E-4	0.0	0.09199130517081294	1.0825235541660764E-4
56-57	3.0156013294626417E-4	8.041603545233711E-4	0.0	0.09697864583107807	1.0825235541660764E-4
58-59	3.0156013294626417E-4	8.157588211751504E-4	0.0	0.10215929426887287	1.0825235541660764E-4
60-61	3.092924440474504E-4	8.273572878269299E-4	0.0	0.10705771335147436	1.159846665177939E-4
62-63	3.092924440474504E-4	8.389557544787093E-4	0.0	0.11177828927874858	1.159846665177939E-4
64-65	3.092924440474504E-4	0.0010361296875589588	0.0	0.11683135458337379	1.159846665177939E-4
66-67	3.092924440474504E-4	0.001039995843109552	0.0	0.12255713095380222	1.159846665177939E-4
68-69	3.092924440474504E-4	0.001070925087514297	0.0	0.12810893032445397	1.159846665177939E-4
70-71	3.092924440474504E-4	0.0010979881763684488	0.0	0.1331001371402697	1.3531544427075956E-4
72-73	3.092924440474504E-4	0.0011057204874696351	0.0	0.13698948962416638	1.6237853312491145E-4
74-75	3.1702475514863666E-4	0.0011366497318743803	0.0	0.14020999719781047	1.6237853312491145E-4
76-77	3.1702475514863666E-4	0.0011637128207285321	0.0	0.14357355252682646	1.6237853312491145E-4
78-79	3.247570662498229E-4	0.0011753112873803115	0.0	0.1456960719241021	1.6237853312491145E-4
80-81	3.247570662498229E-4	0.0011791774429309046	0.0	0.14635331836770293	1.7011084422609773E-4
82-83	3.3635553290160234E-4	0.0011830435984814978	0.0	0.14689458014478596	1.8557546642847025E-4
84-85	3.4022168845219546E-4	0.0011830435984814978	0.0	0.14723480183323817	1.8557546642847025E-4
86-87	3.4022168845219546E-4	0.0012139728428862428	0.0	0.14730439263314885	1.8557546642847025E-4
88	3.4022168845219546E-4	0.0012912959538981055	0.0	0.14731599109980062	1.8557546642847025E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	18215	0.0	50.95307	1
GTATCAA	33220	0.0	38.047966	1
TCAACGC	40530	0.0	30.726301	4
ATCAACG	40590	0.0	30.494255	3
TATCAAC	41710	0.0	29.999521	2
CAACGCA	41485	0.0	29.953703	5
AACGCAG	42865	0.0	29.034483	6
ACGCAGA	49265	0.0	25.219763	7
CGCAGAG	49745	0.0	24.959433	8
GTGGTAT	8385	0.0	24.73705	1
TGGTATC	8460	0.0	23.269392	2
GCAGAGT	57300	0.0	21.609571	9
GAGTACT	35430	0.0	20.186028	12-13
CAGAGTA	55670	0.0	19.382353	10-11
GTACATG	39545	0.0	19.271452	1
TACATGG	39330	0.0	18.882713	2
AGAGTAC	52160	0.0	18.715141	10-11
GTACTTT	39295	0.0	18.200945	14-15
ACATGGG	39655	0.0	18.16335	3
AGTACTT	38115	0.0	17.162964	12-13
>>END_MODULE