FastQCFastQC Report
Fri 27 May 2016
SRR2139901_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139901_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7388051
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA134100.036.8250581
GTATCAA213750.031.939831
TCAACGC239800.027.6573374
ATCAACG239850.027.6166323
CAACGCA245450.027.0003365
AACGCAG252200.026.3423526
TATCAAC261550.025.762572
ACGCAGA291000.022.9173437
CGCAGAG294650.022.6478218
GTACATG216000.021.1171671
TACATGG215850.020.1311682
GAGTACT188850.020.06701512-13
GCAGAGT335850.019.7308449
ACATGGG214500.019.5865553
AGAGTAC275000.018.93865410-11
CAGAGTA309500.018.20933710-11
GTACTTT209100.018.06418614-15
CATGGGG165100.017.7511634
AGTACTT202450.017.31766112-13
ACTTTTT241650.015.76393116-17
Produced by FastQC (version 0.11.5)