##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139901_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7388051
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.71342022408887	33.0	33.0	33.0	33.0	33.0
2	32.70202818036854	33.0	33.0	33.0	33.0	33.0
3	32.70152398785552	33.0	33.0	33.0	33.0	33.0
4	36.62511885746322	37.0	37.0	37.0	37.0	37.0
5	36.64353711147906	37.0	37.0	37.0	37.0	37.0
6	36.56739307836397	37.0	37.0	37.0	37.0	37.0
7	36.61722259361772	37.0	37.0	37.0	37.0	37.0
8	36.62023976282784	37.0	37.0	37.0	37.0	37.0
9	36.62788711122866	37.0	37.0	37.0	37.0	37.0
10-11	36.590484757075984	37.0	37.0	37.0	37.0	37.0
12-13	36.562319751176595	37.0	37.0	37.0	37.0	37.0
14-15	38.64996668268803	40.0	37.0	40.0	37.0	40.0
16-17	38.5977971727591	40.0	37.0	40.0	37.0	40.0
18-19	38.625040013936015	40.0	37.0	40.0	37.0	40.0
20-21	38.64935483764836	40.0	37.0	40.0	37.0	40.0
22-23	38.5026556631078	40.0	37.0	40.0	37.0	40.0
24-25	38.658752469970935	40.0	37.0	40.0	37.0	40.0
26-27	38.44732359925122	40.0	37.0	40.0	37.0	40.0
28-29	38.43665816832097	40.0	37.0	40.0	37.0	40.0
30-31	38.415641718538495	40.0	37.0	40.0	37.0	40.0
32-33	38.4119875380587	40.0	37.0	40.0	37.0	40.0
34-35	38.32221681846114	40.0	37.0	40.0	37.0	40.0
36-37	33.282996543836354	40.0	37.0	40.0	8.5	40.0
38-39	37.24694122869379	40.0	37.0	40.0	33.0	40.0
40-41	37.826600381683235	40.0	37.0	40.0	33.0	40.0
42-43	38.03761642568975	40.0	37.0	40.0	33.0	40.0
44-45	38.04491833650884	40.0	37.0	40.0	33.0	40.0
46-47	37.940063999012715	40.0	37.0	40.0	33.0	40.0
48-49	37.91847153401491	40.0	37.0	40.0	33.0	40.0
50-51	37.87718188833395	40.0	37.0	40.0	33.0	40.0
52-53	37.80676792638256	40.0	37.0	40.0	33.0	40.0
54-55	37.721809958464064	40.0	37.0	40.0	33.0	40.0
56-57	37.646267350781756	40.0	37.0	40.0	33.0	40.0
58-59	37.500724572507615	40.0	37.0	40.0	33.0	40.0
60-61	37.311514520936	37.0	37.0	40.0	33.0	40.0
62-63	37.09004958955239	37.0	37.0	40.0	33.0	40.0
64-65	36.88099235752666	37.0	37.0	40.0	33.0	40.0
66-67	36.61142025855945	37.0	37.0	40.0	33.0	40.0
68-69	36.332361297562926	37.0	37.0	40.0	33.0	40.0
70-71	36.064999524344934	37.0	37.0	38.5	33.0	40.0
72-73	35.8082142220849	37.0	37.0	37.0	33.0	40.0
74-75	35.52529613970839	37.0	33.0	37.0	33.0	40.0
76-77	33.68065582988335	35.0	33.0	37.0	27.0	37.0
78-79	35.15074246923032	37.0	33.0	37.0	33.0	37.0
80-81	35.18419533111298	37.0	33.0	37.0	33.0	37.0
82-83	35.26099636621668	37.0	33.0	37.0	33.0	37.0
84-85	35.10993059590847	37.0	33.0	37.0	33.0	37.0
86-87	35.04069814938179	37.0	33.0	37.0	33.0	37.0
88-89	34.955617992059935	37.0	33.0	37.0	33.0	37.0
90-91	34.82008389992894	37.0	33.0	37.0	33.0	37.0
92-93	34.801833044671525	37.0	33.0	37.0	33.0	37.0
94-95	34.766759328497486	37.0	33.0	37.0	33.0	37.0
96-97	34.74414996812013	37.0	33.0	37.0	33.0	37.0
98-99	34.70499760841098	37.0	33.0	37.0	33.0	37.0
100	34.64808776351059	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	4.0
19	5.0
20	29.0
21	206.0
22	862.0
23	2331.0
24	5738.0
25	11587.0
26	21023.0
27	33737.0
28	50671.0
29	71960.0
30	99284.0
31	133202.0
32	178676.0
33	242952.0
34	340726.0
35	531829.0
36	1014311.0
37	2306880.0
38	2268729.0
39	73308.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.21519315445982	19.89065857829081	14.457845512977643	23.43630275427173
2	14.76383961074443	20.441872964872605	41.707806294244584	23.08648113013838
3	18.78375018256146	25.132841801410223	29.993450121231636	26.089957894796683
4	12.552587066394027	17.508391391133248	38.470225198608105	31.468796343864625
5	13.509000364538487	37.58458125384183	35.254228707358436	13.652189674261242
6	30.8517496698385	37.402557183213816	18.221558026602686	13.524135120345
7	27.354656864171623	32.4868358380309	22.14719416528121	18.011313132516275
8	24.831569246070444	36.740339231551054	20.442955794430766	17.98513572794774
9	25.435016623464023	17.16472991320715	20.99033967145056	36.40991379187826
10-11	24.681286038767194	26.78204982613141	28.15543639317054	20.381227741930854
12-13	25.399851733562752	24.10879405136754	28.579215276126273	21.912138938943436
14-15	22.274534921320928	25.658282542987322	26.45596924006074	25.611213295631014
16-17	21.79132223099164	28.8761474440282	27.12792589006221	22.20460443491795
18-19	21.716349819458475	27.96856031448619	29.048256434613133	21.266833431442205
20-21	22.60112975598478	27.028114493815526	29.038141277838996	21.332614472360696
22-23	22.31824350434878	26.938731553831563	28.872408435640097	21.87061650617956
24-25	22.092636158066398	27.019697534620974	28.98905153892469	21.89861476838794
26-27	22.015078930704647	27.133240222063197	28.930169393041805	21.921511454190355
28-29	21.895460565688946	27.369674996622408	28.93506850415105	21.7997959335376
30-31	22.217685450012535	27.191525194848293	28.796010434636433	21.79477892050274
32-33	21.97195609329366	27.31299404665662	28.761234336590253	21.95381552345947
34-35	22.17705065662155	27.41275635230678	28.670335827645964	21.73985716342571
36-37	22.359384613747476	27.24654365109687	28.519942353171217	21.874129381984435
38-39	21.972332505768442	27.41809723602127	28.75353134801637	21.856038910193913
40-41	22.331116892262738	27.307816220415344	28.502166249192406	21.858900638129512
42-43	22.15851257414077	27.42512422685349	28.333425208863705	22.082937990142035
44-45	22.27174893788054	27.556104118400338	28.083366616829704	22.08878032688942
46-47	22.352864962224597	27.554854162374408	27.808665509792718	22.283615365608274
48-49	22.309526064110194	27.692723421400334	27.78354550623493	22.214205008254538
50-51	22.2970977004815	27.81696538859349	27.639752200898414	22.246184710026593
52-53	22.46740086921353	27.701530743683893	27.52876199936939	22.302306387733182
54-55	22.36926078469765	27.650213509343686	27.6108927312681	22.36963297469057
56-57	22.41048100829067	27.780101965365546	27.50326340204635	22.306153624297433
58-59	22.358814773903752	27.86192338469673	27.505144825532472	22.274117015867045
60-61	22.43569827057283	27.73714284775138	27.574562454948243	22.25259642672755
62-63	22.404688200755622	27.80421390883383	27.47594830716451	22.315149583246033
64-65	22.360476866864488	27.77847285728842	27.481385167937322	22.379665107909766
66-67	22.447934570077525	27.82197028671036	27.341438116975525	22.388657026236586
68-69	22.413621447840693	27.828742165032615	27.379895219394523	22.377741167732168
70-71	22.377458521304753	27.576788236896434	27.475703591880617	22.5700496499182
72-73	22.42574658027697	27.61787251627663	27.472905014546246	22.483475888900152
74-75	22.306331151744487	27.78358647119022	27.383027040119508	22.527055336945782
76-77	22.410156018935222	27.736126720820593	27.322434336635755	22.531282923608437
78-79	22.38647860750705	27.784334196242604	27.269451914961714	22.559735281288635
80-81	22.493475494495588	27.767989243859304	27.240369305455815	22.498165956189293
82-83	22.416002791834806	27.76105242436218	27.431717552362116	22.3912272314409
84-85	22.56400708125816	27.68576115511176	27.333691376964552	22.416540386665528
86-87	22.372464308372642	27.7436165586843	27.39402793438284	22.489891198560215
88-89	22.449165141879362	27.736842809892792	27.33723753449307	22.47675451373478
90-91	22.474852937344245	27.73553169544829	27.369580900576178	22.420034466631282
92-93	22.486038791735556	27.729090469358496	27.374728783358794	22.410141955547154
94-95	22.331495627094274	27.83286165706028	27.399586878969227	22.43605583687622
96-97	22.410462013365397	27.931278017404704	27.477510967060926	22.180749002168973
98-99	23.516102899434145	29.833082295227236	27.664418763299736	18.98639604203888
100	22.438552708443133	30.863892981600245	21.526553109994737	25.171001199961886
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1202.0
1	1118.0
2	1583.0
3	2294.0
4	3154.0
5	5365.5
6	7353.0
7	8701.0
8	9777.0
9	9922.0
10	9422.5
11	8767.0
12	9390.5
13	12386.5
14	19541.5
15	29644.5
16	38667.5
17	44779.5
18	46805.0
19	44918.5
20	40899.5
21	36004.0
22	32452.0
23	32577.5
24	36450.5
25	44181.5
26	54932.5
27	67419.5
28	81026.0
29	96658.0
30	112455.0
31	128042.0
32	145521.5
33	162647.5
34	179460.5
35	195820.0
36	212960.5
37	229209.5
38	242458.0
39	254337.0
40	266749.5
41	276926.0
42	284383.0
43	292400.0
44	298507.0
45	305514.0
46	311744.0
47	315353.5
48	315779.5
49	311086.0
50	304312.0
51	293706.0
52	279049.0
53	264121.5
54	249438.5
55	231121.5
56	211589.5
57	191495.5
58	169868.5
59	148117.0
60	124228.5
61	101259.5
62	81087.5
63	63215.0
64	47739.0
65	35666.5
66	27302.0
67	21270.0
68	16366.5
69	11818.5
70	8167.0
71	5803.0
72	4080.0
73	2692.0
74	1807.0
75	1293.0
76	868.0
77	542.5
78	370.5
79	263.5
80	186.0
81	131.0
82	95.0
83	70.5
84	51.0
85	35.0
86	24.5
87	17.0
88	11.5
89	7.5
90	4.5
91	3.0
92	2.5
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	0.0
3	0.0017325272930573976
4	0.013751935388643093
5	0.008500212031562858
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0016451974047315605
26-27	0.03702031064551339
28-29	0.08492385991818793
30-31	0.08213752391183553
32-33	0.03741621876518866
34-35	0.0012810168766478186
36-37	7.422228346007426
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	7.353730621081381E-6
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.01145341476726944
74-75	0.033229545804242956
76-77	0.004604097198886448
78-79	0.0
80-81	0.0
82-83	0.003256157467775141
84-85	0.03890901996920152
86-87	0.04245926760487816
88-89	0.011615674195843317
90-91	1.7144163038247427E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	235.0
20-21	814.0
22-23	1147.0
24-25	2625.0
26-27	3619.0
28-29	6025.0
30-31	13326.0
32-33	16932.0
34-35	27232.0
36-37	33280.0
38-39	41322.0
40-41	45529.0
42-43	53335.0
44-45	64364.0
46-47	62515.0
48-49	55110.0
50-51	49142.0
52-53	47588.0
54-55	51455.0
56-57	53001.0
58-59	53202.0
60-61	52239.0
62-63	51025.0
64-65	53507.0
66-67	56780.0
68-69	57392.0
70-71	56917.0
72-73	54254.0
74-75	55315.0
76-77	55039.0
78-79	58653.0
80-81	59751.0
82-83	57908.0
84-85	57941.0
86-87	61742.0
88-89	67365.0
90-91	70381.0
92-93	75142.0
94-95	91378.0
96-97	255425.0
98-99	457110.0
100-101	4900989.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.66606414554394
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.09681919593498	79.86099595085855
2	6.420213054926875	11.256696190025561
3	1.5025634650907185	3.951714753401811
4	0.4769713940191461	1.672568192946879
5	0.2116437903750492	0.927698905151256
6	0.11058339906048748	0.5816646813281386
7	0.05549825389802672	0.34057194403330665
8	0.03506482125800991	0.24591958957253834
9	0.021524098458754304	0.16982396965441424
>10	0.06795338480719126	0.8908793493398549
>50	9.014371768930398E-4	0.054355604288633605
>100	2.4156016179367753E-4	0.03148299970851094
>500	2.2144832108558683E-5	0.015627869690575714
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.353536947701092E-5
2	0.0	0.0	0.0	0.0	1.353536947701092E-5
3	0.0	0.0	0.0	0.0	1.353536947701092E-5
4	0.0	1.353536947701092E-5	0.0	0.0	1.353536947701092E-5
5	0.0	1.353536947701092E-5	0.0	0.0	1.353536947701092E-5
6	4.060610843103276E-5	1.353536947701092E-5	0.0	0.0	1.353536947701092E-5
7	4.060610843103276E-5	1.353536947701092E-5	0.0	2.707073895402184E-5	1.353536947701092E-5
8	4.060610843103276E-5	2.707073895402184E-5	0.0	4.060610843103276E-5	1.353536947701092E-5
9	4.060610843103276E-5	8.121221686206552E-5	0.0	4.060610843103276E-5	4.060610843103276E-5
10-11	4.060610843103276E-5	8.121221686206552E-5	0.0	4.060610843103276E-5	4.060610843103276E-5
12-13	4.060610843103276E-5	8.121221686206552E-5	0.0	7.444453212356005E-5	4.060610843103276E-5
14-15	4.060610843103276E-5	1.9626285741665834E-4	0.0	1.4888906424712013E-4	5.414147790804368E-5
16-17	8.121221686206552E-5	2.1656591163217472E-4	0.0	2.3010128110918565E-4	5.414147790804368E-5
18-19	8.121221686206552E-5	2.368689658476911E-4	0.0	3.2484886744826207E-4	6.76768473850546E-5
20-21	8.121221686206552E-5	2.5717202006320745E-4	0.0	5.617178332959531E-4	6.76768473850546E-5
22-23	8.121221686206552E-5	2.9101044375573476E-4	0.0	7.309099517585895E-4	8.121221686206552E-5
24-25	8.121221686206552E-5	3.316165521867675E-4	0.0	9.204051244367426E-4	9.474758633907643E-5
26-27	8.121221686206552E-5	3.519196064022839E-4	0.0	0.0011166679818534009	9.474758633907643E-5
28-29	8.121221686206552E-5	3.586872911407894E-4	0.0	0.0014753552729941902	9.474758633907643E-5
30-31	8.121221686206552E-5	4.195964537873385E-4	0.0	0.0027003062106636787	9.474758633907643E-5
32-33	8.121221686206552E-5	4.195964537873385E-4	0.0	0.004967480598063008	1.0828295581608736E-4
34-35	8.121221686206552E-5	4.8050561643388765E-4	0.0	0.008344555282577232	1.2181832529309828E-4
36-37	8.121221686206552E-5	5.346470943419313E-4	0.0	0.01367072317178103	1.2181832529309828E-4
38-39	8.797990160057097E-5	5.549501485574477E-4	0.0	0.023808714910062208	1.3535369477010918E-4
40-41	9.474758633907643E-5	6.226269959425023E-4	0.0	0.032227714724763	1.6919211846263649E-4
42-43	9.474758633907643E-5	6.293946806810078E-4	0.0	0.0389141872464064	1.8272748793964741E-4
44-45	1.0828295581608735E-4	7.512130059741061E-4	0.0	0.04650076183827101	1.8949517267815286E-4
46-47	1.2181832529309828E-4	8.662636465286989E-4	0.0	0.05372864913899485	1.9626285741665834E-4
48-49	1.2181832529309828E-4	8.730313312672043E-4	0.0	0.06149795121879911	2.1656591163217472E-4
50-51	1.2858601003160373E-4	0.001015152710775819	0.0	0.06825210058782756	2.1656591163217472E-4
52-53	1.353536947701092E-4	0.0010286880802528298	0.0	0.0748708962620859	2.3010128110918565E-4
54-55	1.353536947701092E-4	0.0010354557649913353	0.0	0.0813069644484046	2.4363665058619655E-4
56-57	1.353536947701092E-4	0.0010692941886838627	0.0	0.08673464760868596	2.4363665058619655E-4
58-59	1.353536947701092E-4	0.0010828295581608736	0.0	0.09206758318262828	2.50404335324702E-4
60-61	1.353536947701092E-4	0.00110313261237639	0.0	0.09754264013607919	2.707073895402184E-4
62-63	1.6242443372413103E-4	0.0011369710360689172	0.0	0.10253719147309623	2.707073895402184E-4
64-65	1.6919211846263649E-4	0.0013129308392700592	0.0	0.10793103620968507	2.707073895402184E-4
66-67	1.7595980320114196E-4	0.00132646620874707	0.0	0.11337902242418196	2.707073895402184E-4
68-69	1.7595980320114196E-4	0.0013535369477010919	0.0	0.1189962007571415	2.707073895402184E-4
70-71	1.7595980320114196E-4	0.0013806076866551137	0.0	0.12390953987729647	2.7747507427872386E-4
72-73	1.8949517267815286E-4	0.0014076784256091357	0.0	0.12776712017824457	3.2484886744826207E-4
74-75	1.8949517267815286E-4	0.0014144461103476412	0.0	0.13061631545315538	3.2484886744826207E-4
76-77	1.8949517267815286E-4	0.0014618199035171793	0.0	0.13383096570394545	3.2484886744826207E-4
78-79	1.8949517267815286E-4	0.0014753552729941902	0.0	0.13582743270180458	3.2484886744826207E-4
80-81	1.8949517267815286E-4	0.0014753552729941902	0.0	0.13658541339251717	3.2484886744826207E-4
82-83	1.8949517267815286E-4	0.0014821229577326957	0.0	0.13702531290052006	3.3838423692527297E-4
84-85	1.8949517267815286E-4	0.0014888906424712011	0.0	0.13718096964950566	3.3838423692527297E-4
86-87	1.8949517267815286E-4	0.001515961381425223	0.0	0.13728925260532177	3.3838423692527297E-4
88	1.8949517267815286E-4	0.0016107089677642994	0.0	0.13730278797479878	3.3838423692527297E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13410	0.0	36.825058	1
GTATCAA	21375	0.0	31.93983	1
TCAACGC	23980	0.0	27.657337	4
ATCAACG	23985	0.0	27.616632	3
CAACGCA	24545	0.0	27.000336	5
AACGCAG	25220	0.0	26.342352	6
TATCAAC	26155	0.0	25.76257	2
ACGCAGA	29100	0.0	22.917343	7
CGCAGAG	29465	0.0	22.647821	8
GTACATG	21600	0.0	21.117167	1
TACATGG	21585	0.0	20.131168	2
GAGTACT	18885	0.0	20.067015	12-13
GCAGAGT	33585	0.0	19.730844	9
ACATGGG	21450	0.0	19.586555	3
AGAGTAC	27500	0.0	18.938654	10-11
CAGAGTA	30950	0.0	18.209337	10-11
GTACTTT	20910	0.0	18.064186	14-15
CATGGGG	16510	0.0	17.751163	4
AGTACTT	20245	0.0	17.317661	12-13
ACTTTTT	24165	0.0	15.763931	16-17
>>END_MODULE