FastQCFastQC Report
Fri 27 May 2016
SRR2139900_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139900_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20682267
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA391100.052.559181
GTATCAA699900.034.0263561
TCAACGC894250.026.2998964
TATCAAC898250.026.2626362
ATCAACG894400.026.217063
CAACGCA913650.025.729475
GTGGTAT148700.025.3894541
AACGCAG939500.025.0386266
TGGTATC154150.023.8526672
ACGCAGA1047400.022.4629827
CGCAGAG1059450.022.222378
GCAGAGT1186700.019.6933889
GAGTACT755800.019.62278412-13
ACCGAAC38850.019.4702018
GTACATG728100.018.7493271
CAGAGTA1163400.018.71971310-11
TACATGG724500.018.4617252
GTACTTT828200.017.83188414-15
GGACCGA46200.017.8223326
ACATGGG742250.017.7016683
Produced by FastQC (version 0.11.5)