##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139900_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20682267
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.68671006906545	33.0	33.0	33.0	33.0	33.0
2	32.69756149072053	33.0	33.0	33.0	33.0	33.0
3	32.59097931575876	33.0	33.0	33.0	33.0	33.0
4	36.57303577987848	37.0	37.0	37.0	37.0	37.0
5	36.632055373813714	37.0	37.0	37.0	37.0	37.0
6	36.655661393405275	37.0	37.0	37.0	37.0	37.0
7	36.63640165751656	37.0	37.0	37.0	37.0	37.0
8	36.642820344597624	37.0	37.0	37.0	37.0	37.0
9	36.637416343189074	37.0	37.0	37.0	37.0	37.0
10-11	36.60427761618202	37.0	37.0	37.0	37.0	37.0
12-13	36.57205037049371	37.0	37.0	37.0	37.0	37.0
14-15	38.645472447483634	40.0	37.0	40.0	37.0	40.0
16-17	38.597725626499255	40.0	37.0	40.0	37.0	40.0
18-19	38.63721435856137	40.0	37.0	40.0	37.0	40.0
20-21	38.67035209749109	40.0	37.0	40.0	37.0	40.0
22-23	38.71304525333705	40.0	37.0	40.0	37.0	40.0
24-25	38.728291224625664	40.0	37.0	40.0	37.0	40.0
26-27	38.43198529464452	40.0	37.0	40.0	37.0	40.0
28-29	38.3892935756663	40.0	37.0	40.0	37.0	40.0
30-31	38.38349290793126	40.0	37.0	40.0	37.0	40.0
32-33	38.36767381366862	40.0	37.0	40.0	37.0	40.0
34-35	38.19211786087176	40.0	37.0	40.0	37.0	40.0
36-37	38.25165218313796	40.0	37.0	40.0	37.0	40.0
38-39	38.185374853441814	40.0	37.0	40.0	37.0	40.0
40-41	38.19241720643846	40.0	37.0	40.0	37.0	40.0
42-43	38.14361846066171	40.0	37.0	40.0	37.0	40.0
44-45	38.06350992045316	40.0	37.0	40.0	33.0	40.0
46-47	37.97948758833045	40.0	37.0	40.0	33.0	40.0
48-49	37.75117961184186	40.0	37.0	40.0	33.0	40.0
50-51	37.8586240169283	40.0	37.0	40.0	33.0	40.0
52-53	37.83599419404524	40.0	37.0	40.0	33.0	40.0
54-55	33.38528577665462	37.0	33.0	40.0	10.5	40.0
56-57	36.511188223952786	37.0	35.0	40.0	30.0	40.0
58-59	37.31377891702783	37.0	37.0	40.0	33.0	40.0
60-61	37.35730913991347	37.0	37.0	40.0	33.0	40.0
62-63	37.131039880056164	37.0	37.0	40.0	33.0	40.0
64-65	32.34230321452143	37.0	33.0	40.0	8.5	40.0
66-67	35.32651733712584	37.0	33.0	40.0	30.0	40.0
68-69	36.177819845164045	37.0	37.0	40.0	33.0	40.0
70-71	36.10277136279091	37.0	37.0	38.5	33.0	40.0
72-73	35.47256558023479	37.0	35.0	37.0	33.0	40.0
74-75	35.543583497209134	37.0	33.0	37.0	33.0	40.0
76-77	33.70072431958461	35.0	33.0	37.0	27.0	37.0
78-79	35.24834863420303	37.0	33.0	37.0	33.0	37.0
80-81	35.47027585689571	37.0	33.0	37.0	33.0	37.0
82-83	35.41912252039636	37.0	33.0	37.0	33.0	37.0
84-85	35.310883809242256	37.0	33.0	37.0	33.0	37.0
86-87	35.21577445434381	37.0	33.0	37.0	33.0	37.0
88-89	35.14221464366053	37.0	33.0	37.0	33.0	37.0
90-91	35.057333245369996	37.0	33.0	37.0	33.0	37.0
92-93	34.99980814946274	37.0	33.0	37.0	33.0	37.0
94-95	34.95917631459943	37.0	33.0	37.0	33.0	37.0
96-97	34.93558396334714	37.0	33.0	37.0	33.0	37.0
98-99	34.90417761731008	37.0	33.0	37.0	33.0	37.0
100	34.7916343110024	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	1.0
14	0.0
15	0.0
16	6.0
17	4.0
18	14.0
19	57.0
20	168.0
21	688.0
22	2342.0
23	6579.0
24	15077.0
25	30339.0
26	54190.0
27	88189.0
28	133455.0
29	190974.0
30	264403.0
31	362559.0
32	492964.0
33	677975.0
34	981472.0
35	1586770.0
36	3226094.0
37	5197718.0
38	6995747.0
39	374478.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.2029031447334	20.548187620870483	14.026197908139833	23.222711326256288
2	14.986999731412395	21.937017053016195	41.143424088222446	21.932559127348963
3	17.701661567145415	27.170176697444308	31.621236766065426	23.50692496934485
4	12.507827129778393	18.878768646025627	39.19239059608045	29.421013628115528
5	12.805419903645983	38.174779860904515	35.84112265232356	13.178677583125939
6	28.68523207273323	38.42943514990089	19.203582431441507	13.681750345924378
7	25.254060652735056	33.76532339348116	23.941718958647954	17.038896995135833
8	24.430456508005456	35.83071119963354	21.872767713170308	17.866064579190695
9	26.024574578792546	16.15096642935709	21.571614949173608	36.25284404267675
10-11	24.055794950127805	26.62751750702752	29.357375540958618	19.959312001886058
12-13	25.27490337495401	24.908386493608266	29.06625032932802	20.7504598021097
14-15	22.419827383988828	25.912119052534116	27.384689911493975	24.28336365198308
16-17	21.24659527086544	28.727682204199905	28.0770377050634	21.94868481987125
18-19	21.24977900751632	27.73662912975511	30.217670313615802	20.795921549112766
20-21	22.299756755632234	26.9259763047256	30.08686932751453	20.68739761212763
22-23	22.064629675770828	26.860735342421897	29.93459756995862	21.14003741184865
24-25	21.716700867900865	27.078055153565945	29.998637207646695	21.2066067708865
26-27	21.583390640405135	27.268826081403695	29.84120286684045	21.306580411350716
28-29	21.46281633855348	27.40616301663714	29.862138740695855	21.268881904113524
30-31	21.821464266894303	27.20772462378785	29.709299668851635	21.26151144046621
32-33	21.484311774739442	27.473830792995425	29.67901014289579	21.36284728936934
34-35	21.64260747559849	27.586444388827346	29.672570363776696	21.09837777179747
36-37	21.7152910816613	27.50365939936405	29.51975697590108	21.26129254307357
38-39	21.568085952568964	27.52988720364221	29.478321248505974	21.423705595282854
40-41	21.776319035448804	27.410825139926164	29.369077918144615	21.443777906480413
42-43	21.72498918203692	27.567604196129235	29.028992858657894	21.678413763175953
44-45	21.847048828877384	27.743822502670053	28.741783364668255	21.66734530378431
46-47	21.951912459850416	27.80233603588405	28.314408862513936	21.9313426417516
48-49	21.911441339387817	28.05555645203801	28.080888356736907	21.952113851837268
50-51	21.97041791922108	28.106678205630992	27.913183815412385	22.009720059735542
52-53	22.116817277897024	27.90916276855305	27.821811934564643	22.152208018985284
54-55	23.052115698802282	27.769216067721075	27.295709529056484	21.882958704420155
56-57	22.06154315727142	28.021162974402603	27.81003833635024	22.107255531975735
58-59	22.022811134786803	28.105498150138324	27.769699573062763	22.10199114201211
60-61	22.163492592248023	27.991685451038734	27.767547345137693	22.077274611575547
62-63	22.07991138006352	28.041822707399678	27.737495643986946	22.140770268549854
64-65	22.543817156390066	28.00468752300855	27.380454580306363	22.07104074029503
66-67	22.204762526481197	28.066694260420793	27.549792160054597	22.17875105304341
68-69	22.165420077584827	28.01572896636627	27.620375501642076	22.198475454406825
70-71	22.150602978729825	27.779572065141316	27.66656188644468	22.403263069684183
72-73	22.32326856090131	27.783052614373904	27.559863792352974	22.33381503237181
74-75	22.092125250036286	27.89511392976078	27.58628451561156	22.42647630459138
76-77	22.158579614306507	27.77381222502189	27.603880252637197	22.463727908034407
78-79	22.241528877743775	27.832473257732037	27.52212397100794	22.403873893516245
80-81	22.276971444772567	27.755469841925873	27.539266279405634	22.42829243389593
82-83	22.314877139688342	27.63960192090544	27.644485060425637	22.40103587898058
84-85	22.451767701099858	27.59310675428229	27.54552351874046	22.409602025877394
86-87	22.222977671795565	27.62725064903342	27.664332099409688	22.48543957976133
88-89	22.33340026158106	27.540525469473188	27.60735595820935	22.518718310736404
90-91	22.434669642971272	27.473778491803834	27.64119936344637	22.450352501778525
92-93	22.48179818614417	27.34898358422172	27.6554183635925	22.51379986604161
94-95	22.339352515858977	27.33041932529862	27.776025916898984	22.55420224194342
96-97	22.430390147582134	27.44410640323859	27.823427499897274	22.302075949282003
98-99	23.503979668848025	29.397331797871463	27.987092668983344	19.11159586429716
100	30.01358078355964	40.69511263700505	29.291306579435307	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	12271.0
1	10652.5
2	12018.0
3	15763.0
4	19966.5
5	31058.5
6	41429.0
7	49354.5
8	55740.5
9	57369.0
10	54479.5
11	49568.5
12	51518.5
13	63503.0
14	93417.0
15	136732.5
16	173280.0
17	194797.0
18	201607.5
19	195751.0
20	180226.0
21	160270.0
22	144747.0
23	141924.0
24	153912.5
25	179790.0
26	216912.0
27	257584.0
28	299972.0
29	349495.5
30	399315.5
31	446962.0
32	497471.5
33	546811.5
34	593929.0
35	637477.5
36	683963.0
37	726343.5
38	759633.5
39	789077.0
40	816483.0
41	836382.5
42	844440.0
43	855825.5
44	870708.0
45	880259.5
46	883452.0
47	883367.5
48	875579.5
49	857219.0
50	835635.5
51	810193.0
52	775767.0
53	738435.5
54	698370.5
55	654601.0
56	607534.0
57	552802.5
58	493064.5
59	427861.0
60	362957.0
61	300242.5
62	238648.0
63	187424.0
64	147511.5
65	117862.5
66	94185.0
67	73672.5
68	57361.0
69	41339.0
70	28392.0
71	20680.0
72	14914.5
73	10709.0
74	7657.5
75	5411.5
76	3709.0
77	2450.0
78	1622.0
79	1084.0
80	751.0
81	528.0
82	357.0
83	237.0
84	173.0
85	137.5
86	110.5
87	79.5
88	56.5
89	42.0
90	26.5
91	14.5
92	8.5
93	4.5
94	1.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	2.417529954525778E-5
2	9.670119818103112E-6
3	0.0028381801666132636
4	0.012532475284261633
5	0.017033916059588632
6	4.835059909051556E-6
7	4.835059909051556E-6
8	4.835059909051556E-6
9	0.0
10-11	4.835059909051556E-6
12-13	0.0
14-15	1.208764977262889E-5
16-17	2.417529954525778E-5
18-19	1.208764977262889E-5
20-21	0.0
22-23	2.4178793863112627E-6
24-25	0.0017294634021718625
26-27	0.03546864140724232
28-29	0.08255177934041455
30-31	0.08075544801762995
32-33	0.0354455837476132
34-35	8.682601611579157E-4
36-37	7.413067696430726E-6
38-39	3.497645472509043E-5
40-41	1.5181173644389509E-5
42-43	2.562890973823477E-6
44-45	1.5612814686038007E-5
46-47	0.0
48-49	1.882612798695683E-5
50-51	4.094539980071055E-5
52-53	0.0
54-55	1.2312165105548025
56-57	5.1135561737781844E-5
58-59	0.0
60-61	0.0
62-63	0.0
64-65	5.173141449629445
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.009181694889013381
74-75	0.03139288412351224
76-77	0.005095948991367039
78-79	0.0
80-81	0.0
82-83	0.004358142412347208
84-85	0.042186794793979035
86-87	0.04627187286368288
88-89	0.01552660424624769
90-91	1.4950292147396426E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	523.0
20-21	1702.0
22-23	4085.0
24-25	15222.0
26-27	21389.0
28-29	33050.0
30-31	84441.0
32-33	105791.0
34-35	135774.0
36-37	203111.0
38-39	254036.0
40-41	247813.0
42-43	281131.0
44-45	320536.0
46-47	310778.0
48-49	281250.0
50-51	250607.0
52-53	242123.0
54-55	229281.0
56-57	237860.0
58-59	251371.0
60-61	249706.0
62-63	248720.0
64-65	232531.0
66-67	244052.0
68-69	257219.0
70-71	259186.0
72-73	243302.0
74-75	239301.0
76-77	233051.0
78-79	249653.0
80-81	262812.0
82-83	253290.0
84-85	246678.0
86-87	246500.0
88-89	258012.0
90-91	272033.0
92-93	278898.0
94-95	297083.0
96-97	618456.0
98-99	3836050.0
100-101	8143860.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.97680246048974
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.49855401081396	71.72851677722988
2	8.66912110461767	14.213336565986106
3	2.1054078982886826	5.177838221302987
4	0.7628101678543361	2.5013095378019172
5	0.3618181922481062	1.483034923626725
6	0.19619271644996375	0.9649950936363332
7	0.11409591483515177	0.6547252790393069
8	0.0775963029570793	0.5088877439341444
9	0.04916836622433344	0.3627598900749478
>10	0.16084620175840006	2.0086148594539344
>50	0.003473906236270386	0.1901648388937616
>100	8.278000174984623E-4	0.1161924511953524
>500	3.2886942714386415E-5	0.017250909742679326
>1k	5.453075585734863E-5	0.07237290808195304
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.835059909051556E-6	0.0
2	0.0	1.9340239636206225E-5	0.0	4.835059909051556E-6	0.0
3	0.0	1.9340239636206225E-5	0.0	1.4505179727154669E-5	0.0
4	0.0	6.285577881767023E-5	0.0	1.4505179727154669E-5	0.0
5	0.0	7.252589863577334E-5	0.0	1.4505179727154669E-5	0.0
6	0.0	7.252589863577334E-5	0.0	3.384541936336089E-5	9.670119818103112E-6
7	0.0	7.73609585448249E-5	0.0	5.3185658999567115E-5	1.9340239636206225E-5
8	0.0	8.7031078362928E-5	0.0	5.8020718908618674E-5	2.417529954525778E-5
9	0.0	1.4988685718059824E-4	0.0	9.186613827197956E-5	3.384541936336089E-5
10-11	0.0	1.547219170896498E-4	0.0	1.0637131799913423E-4	3.384541936336089E-5
12-13	0.0	1.5955697699870136E-4	0.0	1.2087649772628891E-4	3.384541936336089E-5
14-15	0.0	1.95819926316588E-4	0.0	1.2812908758986623E-4	4.593306913598978E-5
16-17	0.0	2.0307251618016537E-4	0.0	1.52304387135124E-4	5.8020718908618674E-5
18-19	0.0	2.0790757608921693E-4	0.0	2.2966534567994893E-4	6.527330877219601E-5
20-21	0.0	2.1757769590732002E-4	0.0	2.9252112449761915E-4	6.769083872672178E-5
22-23	4.835059909051556E-6	2.5867570513425826E-4	0.0	3.6988208304244405E-4	7.494342859029912E-5
24-25	4.835059909051556E-6	2.755984148159387E-4	0.0	6.237227282676508E-4	9.186613827197956E-5
26-27	4.835059909051556E-6	2.8285100467951603E-4	0.0	0.0012208526270355178	9.186613827197956E-5
28-29	4.835059909051556E-6	2.9010359454309336E-4	0.0	0.003952661475649647	1.0637131799913423E-4
30-31	7.252589863577334E-6	3.5779443326981514E-4	0.0	0.009534738140649668	1.3538167745344356E-4
32-33	1.4505179727154669E-5	3.674645530879183E-4	0.0	0.017776097755628047	1.547219170896498E-4
34-35	1.4505179727154669E-5	4.061450323603307E-4	0.0	0.0300668200444371	1.6439203690775292E-4
36-37	1.4505179727154669E-5	4.3757292176916583E-4	0.0	0.0487108110537399	1.6439203690775292E-4
38-39	1.4505179727154669E-5	4.859235208596814E-4	0.0	0.06986903321574951	1.6439203690775292E-4
40-41	1.4505179727154669E-5	5.173514102685165E-4	0.0	0.08828819393928142	1.668095668622787E-4
42-43	1.4505179727154669E-5	5.246040001320939E-4	0.0	0.10590473471791076	1.7889721663490757E-4
44-45	1.4505179727154669E-5	5.536143595864032E-4	2.417529954525778E-6	0.11975959888729798	1.885673364530107E-4
46-47	1.4505179727154669E-5	5.802071890861867E-4	4.835059909051556E-6	0.13237185266005896	2.0307251618016534E-4
48-49	1.4505179727154669E-5	5.898773089042899E-4	4.835059909051556E-6	0.14383336217446568	2.2724781572542314E-4
50-51	1.4505179727154669E-5	6.527330877219601E-4	4.835059909051556E-6	0.15555112986405215	2.2724781572542314E-4
52-53	1.4505179727154669E-5	6.527330877219601E-4	4.835059909051556E-6	0.16694011347982307	2.4175299545257782E-4
54-55	1.4505179727154669E-5	6.624032075400633E-4	4.835059909051556E-6	0.17592848985074994	2.5867570513425826E-4
56-57	1.4505179727154669E-5	6.817434471762694E-4	4.835059909051556E-6	0.18345909565909774	2.6351076504330985E-4
58-59	1.4505179727154669E-5	6.86578507085321E-4	4.835059909051556E-6	0.19080355166094704	2.683458249523613E-4
60-61	1.4505179727154669E-5	7.059187467215271E-4	4.835059909051556E-6	0.19850580209606616	2.755984148159387E-4
62-63	1.4505179727154669E-5	7.155888665396303E-4	4.835059909051556E-6	0.2059638820057782	2.755984148159387E-4
64-65	1.4505179727154669E-5	7.542693458120427E-4	4.835059909051556E-6	0.21288285273563096	2.755984148159387E-4
66-67	1.4505179727154669E-5	7.687745255391974E-4	4.835059909051556E-6	0.22099608326301948	2.804334747249903E-4
68-69	1.4505179727154669E-5	8.002024149480326E-4	4.835059909051556E-6	0.22899085482263623	2.852685346340418E-4
70-71	1.4505179727154669E-5	8.122900647206615E-4	4.835059909051556E-6	0.2364634399120754	2.8768606458856756E-4
72-73	1.4505179727154669E-5	8.122900647206615E-4	4.835059909051556E-6	0.2424830894988446	3.1427889408835113E-4
74-75	1.4505179727154669E-5	8.316303043568677E-4	4.835059909051556E-6	0.24695310238476276	3.1427889408835113E-4
76-77	1.4505179727154669E-5	8.678932536747544E-4	4.835059909051556E-6	0.251152351915774	3.166964240428769E-4
78-79	1.4505179727154669E-5	8.751458435383317E-4	4.835059909051556E-6	0.2538478978150703	3.191139539974027E-4
80-81	1.4505179727154669E-5	8.799809034473832E-4	4.835059909051556E-6	0.2548052396770625	3.312016037700316E-4
82-83	1.4505179727154669E-5	8.896510232654863E-4	4.835059909051556E-6	0.2555691791426926	3.529593733607636E-4
84-85	1.4505179727154669E-5	9.210789126743214E-4	4.835059909051556E-6	0.2559052158063717	3.529593733607636E-4
86-87	1.4505179727154669E-5	9.47671742174105E-4	4.835059909051556E-6	0.25605993772346136	3.529593733607636E-4
88	1.4505179727154669E-5	9.621769219012597E-4	4.835059909051556E-6	0.25607927796309754	3.529593733607636E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	39110	0.0	52.55918	1
GTATCAA	69990	0.0	34.026356	1
TCAACGC	89425	0.0	26.299896	4
TATCAAC	89825	0.0	26.262636	2
ATCAACG	89440	0.0	26.21706	3
CAACGCA	91365	0.0	25.72947	5
GTGGTAT	14870	0.0	25.389454	1
AACGCAG	93950	0.0	25.038626	6
TGGTATC	15415	0.0	23.852667	2
ACGCAGA	104740	0.0	22.462982	7
CGCAGAG	105945	0.0	22.22237	8
GCAGAGT	118670	0.0	19.693388	9
GAGTACT	75580	0.0	19.622784	12-13
ACCGAAC	3885	0.0	19.470201	8
GTACATG	72810	0.0	18.749327	1
CAGAGTA	116340	0.0	18.719713	10-11
TACATGG	72450	0.0	18.461725	2
GTACTTT	82820	0.0	17.831884	14-15
GGACCGA	4620	0.0	17.822332	6
ACATGGG	74225	0.0	17.701668	3
>>END_MODULE