FastQCFastQC Report
Fri 27 May 2016
SRR2139899_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139899_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21148954
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA319850.052.5377241
GTATCAA588500.038.3199961
ATCAACG722150.030.587363
TCAACGC723200.030.559984
TATCAAC743800.029.9424292
CAACGCA739650.029.8541895
AACGCAG760950.029.0495916
ACGCAGA875350.025.2288427
GTGGTAT147150.025.0663831
TGGTATC139050.025.061342
CGCAGAG889450.024.8384468
GCAGAGT1020600.021.5302099
GTACATG730000.021.0076391
TACATGG723950.020.8490052
GAGTACT604700.020.6078112-13
ACATGGG741650.019.7733943
CAGAGTA993050.019.3989710-11
GTACTTT662100.018.7927314-15
AGAGTAC934000.018.73985510-11
CATGGGG540650.017.805364
Produced by FastQC (version 0.11.5)