##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139899_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21148954
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.714512074687	33.0	33.0	33.0	33.0	33.0
2	32.70572071791352	33.0	33.0	33.0	33.0	33.0
3	32.702726338144195	33.0	33.0	33.0	33.0	33.0
4	36.62858130004916	37.0	37.0	37.0	37.0	37.0
5	36.64806273634147	37.0	37.0	37.0	37.0	37.0
6	36.57298951995451	37.0	37.0	37.0	37.0	37.0
7	36.62146714206291	37.0	37.0	37.0	37.0	37.0
8	36.625598079224154	37.0	37.0	37.0	37.0	37.0
9	36.63159837597642	37.0	37.0	37.0	37.0	37.0
10-11	36.59410957156557	37.0	37.0	37.0	37.0	37.0
12-13	36.5645186991281	37.0	37.0	37.0	37.0	37.0
14-15	38.660341121362315	40.0	37.0	40.0	37.0	40.0
16-17	38.61015133419837	40.0	37.0	40.0	37.0	40.0
18-19	38.64437936268621	40.0	37.0	40.0	37.0	40.0
20-21	38.67156479514598	40.0	37.0	40.0	37.0	40.0
22-23	38.52730187548532	40.0	37.0	40.0	37.0	40.0
24-25	38.68026013982918	40.0	37.0	40.0	37.0	40.0
26-27	38.47115807549558	40.0	37.0	40.0	37.0	40.0
28-29	38.458997051786	40.0	37.0	40.0	37.0	40.0
30-31	38.43495269043851	40.0	37.0	40.0	37.0	40.0
32-33	38.428195018281926	40.0	37.0	40.0	37.0	40.0
34-35	38.33991409236354	40.0	37.0	40.0	37.0	40.0
36-37	33.28615528598928	40.0	37.0	40.0	8.5	40.0
38-39	37.266892486265	40.0	37.0	40.0	33.0	40.0
40-41	37.84156750316282	40.0	37.0	40.0	33.0	40.0
42-43	38.05264990091287	40.0	37.0	40.0	33.0	40.0
44-45	38.058173939315	40.0	37.0	40.0	33.0	40.0
46-47	37.952403987158405	40.0	37.0	40.0	33.0	40.0
48-49	37.932920111887924	40.0	37.0	40.0	33.0	40.0
50-51	37.892466089799846	40.0	37.0	40.0	33.0	40.0
52-53	37.825285418318614	40.0	37.0	40.0	33.0	40.0
54-55	37.745390462557495	40.0	37.0	40.0	33.0	40.0
56-57	37.67225383708296	40.0	37.0	40.0	33.0	40.0
58-59	37.53213150348617	40.0	37.0	40.0	33.0	40.0
60-61	37.34381465626997	38.5	37.0	40.0	33.0	40.0
62-63	37.12402333299886	37.0	37.0	40.0	33.0	40.0
64-65	36.91693563725006	37.0	37.0	40.0	33.0	40.0
66-67	36.65091496265548	37.0	37.0	40.0	33.0	40.0
68-69	36.37553723206477	37.0	37.0	40.0	33.0	40.0
70-71	36.1072454722669	37.0	37.0	38.5	33.0	40.0
72-73	35.84923742087189	37.0	37.0	37.0	33.0	40.0
74-75	35.56722625587095	37.0	33.0	37.0	33.0	40.0
76-77	33.72964911899328	35.0	33.0	37.0	27.0	37.0
78-79	35.19061792674432	37.0	33.0	37.0	33.0	37.0
80-81	35.21775881648866	37.0	33.0	37.0	33.0	37.0
82-83	35.29128445228807	37.0	33.0	37.0	33.0	37.0
84-85	35.13812826070804	37.0	33.0	37.0	33.0	37.0
86-87	35.068324698413875	37.0	33.0	37.0	33.0	37.0
88-89	34.98185508036136	37.0	33.0	37.0	33.0	37.0
90-91	34.84415422284883	37.0	33.0	37.0	33.0	37.0
92-93	34.8254497422531	37.0	33.0	37.0	33.0	37.0
94-95	34.78834889619655	37.0	33.0	37.0	33.0	37.0
96-97	34.765317109332415	37.0	33.0	37.0	33.0	37.0
98-99	34.72611828822858	37.0	33.0	37.0	33.0	37.0
100	34.6719220173466	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	21.0
20	136.0
21	667.0
22	2470.0
23	7072.0
24	16672.0
25	33955.0
26	60063.0
27	97313.0
28	145681.0
29	204778.0
30	279981.0
31	378246.0
32	502546.0
33	682617.0
34	959715.0
35	1488312.0
36	2863555.0
37	6587746.0
38	6618568.0
39	218837.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.96595727618491	19.983829933149412	14.80258550848425	23.247627282181426
2	14.63932447912081	20.516499302991534	41.993537836433894	22.85063838145376
3	18.62906366420654	25.390996553707485	30.0791487859862	25.900790996099776
4	12.503546748605702	17.762041353386305	38.517041985180455	31.217369912827543
5	13.471437441274425	37.69503038006648	35.16010949969949	13.673422678959609
6	30.45063126999094	37.50911747219271	18.409534580291773	13.630716677524571
7	27.162690882962814	32.73262119724692	22.139444816041493	17.965243103748772
8	24.83465139694379	36.790784073765536	20.4206978747034	17.953866654587266
9	25.599308599375647	17.244507695274198	20.992565400633996	36.16361830471615
10-11	24.550221254441237	26.98029651962929	28.077305856355828	20.392176369573644
12-13	25.34666253470503	24.339390496570186	28.567339547856598	21.74660742086819
14-15	22.249923093123186	25.833348542911388	26.557159753621857	25.359568610343565
16-17	21.618804409901312	29.050176665947642	27.210712170445877	22.120306753705172
18-19	21.554318951187845	27.96588663439336	29.351401965317052	21.12839244910174
20-21	22.525272086319767	27.00192682864725	29.351530510594813	21.12127057443817
22-23	22.256086394978027	26.91150603068393	29.19201898608849	21.64038858824955
24-25	21.90951536756936	27.038311647848168	29.355595881517658	21.69657710306481
26-27	21.8359798591801	27.20918979335914	29.181762153251068	21.773068194209692
28-29	21.700467953683393	27.394528979573508	29.257631670865013	21.64737139587809
30-31	22.070676285675546	27.17978706018241	29.11197325149309	21.637563402648954
32-33	21.80771457813498	27.316056594329396	29.088096240834567	21.78813258670106
34-35	22.00035122829273	27.396786960533063	29.027229423015466	21.575632388158745
36-37	22.16326790398152	27.304049596480485	28.81707073141352	21.715611768124475
38-39	21.763320612432288	27.461118180588556	29.044980477222595	21.73058072975656
40-41	22.163281130192605	27.345656081562524	28.767971478619543	21.723091309625325
42-43	21.99144660459657	27.450107277368534	28.618777871958752	21.939668246076145
44-45	22.13654652755129	27.611473037081474	28.35876696599619	21.893213469371045
46-47	22.133659501232724	27.667156499758782	28.031716900595168	22.167467098413326
48-49	22.10466489298099	27.78792996930926	27.99369743712384	22.113707700585913
50-51	22.13504336016488	27.909681735162533	27.820610517566518	22.13466438710607
52-53	22.28334862630459	27.827199152639263	27.70111836406014	22.188333856996
54-55	22.24046314719246	27.735581647743658	27.765227390529525	22.25872781453436
56-57	22.218111957844933	27.869410879254563	27.711299653723962	22.201177509176542
58-59	22.207381336343218	27.92750974355347	27.72609800776231	22.139010912341
60-61	22.290337071729994	27.85579588493673	27.738058086477995	22.115808956855282
62-63	22.234814808602007	27.906516296507533	27.66690972338712	22.19175917150334
64-65	22.2335638150508	27.897679331942182	27.62992994969486	22.238826903312155
66-67	22.318629234760714	27.917947955068108	27.50401074960493	22.25941206056625
68-69	22.24714717486508	27.899615800272198	27.57814458588093	22.27509243898179
70-71	22.234887683598416	27.72283724316153	27.62200099774697	22.420274075493083
72-73	22.274722695221467	27.7810575588745	27.585788430210577	22.35843131569346
74-75	22.176644044363375	27.892800794165325	27.5204283635541	22.4101267979172
76-77	22.27295384773835	27.859897941605542	27.463553529549568	22.403594681106544
78-79	22.24371341332289	27.892401458949024	27.445806712370313	22.41807841535777
80-81	22.332604185653658	27.87904001384669	27.40988163146004	22.378474169039613
82-83	22.293440674350826	27.87673644226801	27.56351381834875	22.26630906503241
84-85	22.44347791795288	27.8244494290916	27.436053796829412	22.2960188561261
86-87	22.252217093378334	27.873804343261092	27.530716479140434	22.34326208422014
88-89	22.2822536953956	27.857119945417764	27.499275340566413	22.361351018620226
90-91	22.32504074489836	27.866468825525608	27.5112854039829	22.297205025593133
92-93	22.296829843005515	27.85423576840065	27.56778587827831	22.281148510315525
94-95	22.207532189718826	27.927891028538497	27.576838864745095	22.28773791699758
96-97	22.282437648638336	28.07286759426385	27.60223568032478	22.04245907677303
98-99	23.382647407595897	29.952223582381986	27.813129114181596	18.851999895840514
100	22.27588434938932	30.9858183152348	21.71521259802442	25.023084737351464
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4766.0
1	4200.5
2	5366.5
3	7574.5
4	10073.5
5	16541.5
6	22425.5
7	26872.5
8	30834.5
9	31887.5
10	30397.5
11	28309.5
12	30203.5
13	39565.5
14	62680.0
15	95645.0
16	125395.5
17	145272.5
18	151661.0
19	144691.5
20	130161.0
21	113561.5
22	101510.0
23	100420.0
24	111043.5
25	134081.5
26	166795.5
27	204199.0
28	243893.5
29	288672.0
30	335105.0
31	381962.0
32	432361.5
33	481419.5
34	531033.5
35	576360.5
36	624624.5
37	672168.0
38	705080.0
39	731421.5
40	761114.0
41	788221.0
42	806150.0
43	822347.0
44	836580.5
45	851512.5
46	861563.5
47	863853.5
48	860782.5
49	847880.5
50	830309.0
51	806302.0
52	771731.0
53	733459.0
54	693906.5
55	645004.5
56	591999.5
57	535085.5
58	475294.0
59	415409.0
60	350117.5
61	287304.5
62	229099.0
63	177447.0
64	133805.0
65	100435.5
66	76702.5
67	59007.0
68	44735.5
69	31654.0
70	22254.5
71	16319.5
72	11874.0
73	8409.5
74	5815.5
75	4022.5
76	2741.5
77	1814.0
78	1187.5
79	800.5
80	530.0
81	351.5
82	248.5
83	183.0
84	134.5
85	95.0
86	74.5
87	56.0
88	33.0
89	22.0
90	16.5
91	10.5
92	7.5
93	5.5
94	3.5
95	3.0
96	3.0
97	3.0
98	2.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	0.0
3	0.0020331974810669125
4	0.01336236298022115
5	0.008813674662113313
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0015371294202669095
26-27	0.03649602919985129
28-29	0.08404386185036083
30-31	0.08133036203062519
32-33	0.03662446854827485
34-35	0.0012893746960929567
36-37	7.440957518460727
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	2.798207305595247E-5
56-57	4.612194514178975E-5
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.011082713970391839
74-75	0.03318664036006031
76-77	0.004335618897932337
78-79	0.0
80-81	0.0
82-83	0.0032803532023955658
84-85	0.038904272868305334
86-87	0.04200356236058391
88-89	0.01189714097033164
90-91	2.9732654378259722E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	281.0
20-21	1125.0
22-23	2396.0
24-25	6552.0
26-27	8914.0
28-29	15663.0
30-31	35787.0
32-33	45729.0
34-35	74140.0
36-37	89351.0
38-39	111899.0
40-41	127210.0
42-43	153368.0
44-45	185993.0
46-47	179311.0
48-49	154416.0
50-51	136028.0
52-53	131117.0
54-55	139876.0
56-57	145794.0
58-59	147347.0
60-61	145138.0
62-63	141178.0
64-65	149537.0
66-67	157125.0
68-69	160206.0
70-71	157764.0
72-73	151576.0
74-75	154558.0
76-77	153877.0
78-79	164535.0
80-81	167063.0
82-83	162234.0
84-85	162856.0
86-87	174280.0
88-89	188754.0
90-91	199104.0
92-93	212199.0
94-95	260106.0
96-97	729013.0
98-99	1319844.0
100-101	1.414571E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.03995651435152
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.54366737885356	73.52662288761202
2	7.679655758339974	12.754365604354815
3	2.057860038789931	5.126538244012126
4	0.7832723823732365	2.6017161828466424
5	0.34814007994533536	1.445476854978175
6	0.20788694978100195	1.0357753961829346
7	0.11571711326626823	0.6726400837518041
8	0.0714339383340023	0.47454969063235347
9	0.047510653220897044	0.3550754319688552
>10	0.14180434219269134	1.7277146931366474
>50	0.0023339622526298695	0.13254434326599068
>100	6.672501714796007E-4	0.09666472085625062
>500	1.0073095878251939E-5	0.005211204634219441
>1k	4.007938313877849E-5	0.04510466176722282
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.728366235039331E-6
2	0.0	0.0	0.0	4.255529611535398E-5	4.728366235039331E-6
3	0.0	4.728366235039331E-6	0.0	4.255529611535398E-5	4.728366235039331E-6
4	0.0	1.4185098705117993E-5	0.0	6.619712729055063E-5	4.728366235039331E-6
5	0.0	1.4185098705117993E-5	0.0	7.092549352558996E-5	4.728366235039331E-6
6	7.092549352558996E-5	1.4185098705117993E-5	0.0	7.092549352558996E-5	9.456732470078662E-6
7	7.092549352558996E-5	1.4185098705117993E-5	0.0	7.56538597606293E-5	1.4185098705117993E-5
8	7.092549352558996E-5	1.8913464940157324E-5	0.0	7.56538597606293E-5	1.4185098705117993E-5
9	7.092549352558996E-5	5.674039482047197E-5	0.0	8.511059223070796E-5	1.4185098705117993E-5
10-11	7.092549352558996E-5	5.674039482047197E-5	0.0	1.229375221110226E-4	1.4185098705117993E-5
12-13	7.092549352558996E-5	5.674039482047197E-5	0.0	1.631286351088569E-4	1.4185098705117993E-5
14-15	7.092549352558996E-5	8.983895846574728E-5	0.0	1.7731373381397493E-4	2.3641831175196655E-5
16-17	1.3948680393366026E-4	8.983895846574728E-5	0.0	2.1041229745925023E-4	3.309856364527532E-5
18-19	1.4185098705117992E-4	9.693150781830628E-5	0.0	2.6478850916220254E-4	4.019111299783431E-5
20-21	1.4185098705117992E-4	1.0402405717086528E-4	0.0	2.7660942474980085E-4	4.728366235039331E-5
22-23	1.4894353640373893E-4	1.2766588834606192E-4	0.0	3.215289039826745E-4	4.964784546791297E-5
24-25	1.5840026887381758E-4	1.4894353640373893E-4	0.0	3.5462746762794986E-4	5.201202858543264E-5
26-27	1.6076445199133724E-4	1.5603608575629792E-4	0.0	4.0427531309586277E-4	5.201202858543264E-5
28-29	1.654928182263766E-4	1.5603608575629792E-4	0.0	6.028666949675147E-4	5.9104577937991635E-5
30-31	1.6785700134389626E-4	1.773137338139749E-4	0.0	0.001489435364037389	6.14687610555113E-5
32-33	1.7022118446141592E-4	1.9386301563661257E-4	0.0	0.0033902385905232	6.619712729055063E-5
34-35	1.7494955069645524E-4	2.1750484681180924E-4	0.0	0.005969562371737155	6.619712729055063E-5
36-37	1.8204210004901426E-4	2.411466779870059E-4	0.0	0.010236912898860152	7.092549352558996E-5
38-39	1.8440628316653392E-4	2.458750442220452E-4	0.0	0.018915829123274844	8.511059223070795E-5
40-41	1.8913464940157324E-4	2.742452416322812E-4	0.0	0.02581215127707971	1.0875242340590462E-4
42-43	1.8913464940157324E-4	2.8133779098484023E-4	0.0	0.0318219047618147	1.1820915587598327E-4
44-45	1.985913818716519E-4	3.073438052775565E-4	0.0	0.03846998768828	1.229375221110226E-4
46-47	2.0804811434173057E-4	3.215289039826745E-4	0.0	0.04575167169024057	1.2766588834606195E-4
48-49	2.0804811434173057E-4	3.262572702177138E-4	0.0	0.052487229392054094	1.3948680393366026E-4
50-51	2.1750484681180922E-4	3.498991013929105E-4	0.0	0.05875704301971625	1.5603608575629792E-4
52-53	2.1750484681180922E-4	3.546274676279498E-4	0.0	0.06514742998637191	1.6549281822637657E-4
54-55	2.1750484681180922E-4	3.546274676279498E-4	0.0	0.07125647916204272	1.7494955069645524E-4
56-57	2.1750484681180922E-4	3.546274676279498E-4	0.0	0.07646950293617358	1.9386301563661257E-4
58-59	2.1750484681180922E-4	3.5699165074546947E-4	0.0	0.08163524304795405	2.0331974810669122E-4
60-61	2.3641831175196654E-4	3.664483832155482E-4	0.0	0.08647709007263432	2.1750484681180922E-4
62-63	2.600601429271632E-4	3.782692988031465E-4	0.0	0.09127638180119924	2.2932576239940755E-4
64-65	2.6478850916220254E-4	4.4446642609369713E-4	0.0	0.09593382254271299	2.3168994551692722E-4
66-67	2.718810585147615E-4	4.5628734168129544E-4	0.0	0.10077803375051078	2.3641831175196654E-4
68-69	2.742452416322812E-4	4.846575390915314E-4	0.0	0.10576882431159479	2.4587504422204524E-4
70-71	2.837019741023599E-4	4.917500884440904E-4	0.0	0.11027022896735225	2.671526922797222E-4
72-73	3.1207217151259584E-4	4.917500884440904E-4	0.0	0.11386615148909965	2.8370197410235984E-4
74-75	3.1207217151259584E-4	4.964784546791297E-4	0.0	0.11675518325870868	2.8606615721987955E-4
76-77	3.1207217151259584E-4	5.106635533842478E-4	0.0	0.11945980874515118	2.884303403373992E-4
78-79	3.1207217151259584E-4	5.224844689718461E-4	0.0	0.12125185954823109	2.884303403373992E-4
80-81	3.1207217151259584E-4	5.248486520893658E-4	0.0	0.12194456520166434	3.026154390425172E-4
82-83	3.144363546301155E-4	5.248486520893658E-4	0.0	0.1223961241771106	3.215289039826745E-4
84-85	3.191647208651548E-4	5.343053845594444E-4	0.0	0.12263727085509761	3.215289039826745E-4
86-87	3.2389308710019415E-4	5.343053845594444E-4	0.0	0.12271765308109328	3.2625727021771386E-4
88	3.309856364527532E-4	5.721323144397591E-4	0.0	0.12272474563044583	3.357140026877925E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	31985	0.0	52.537724	1
GTATCAA	58850	0.0	38.319996	1
ATCAACG	72215	0.0	30.58736	3
TCAACGC	72320	0.0	30.55998	4
TATCAAC	74380	0.0	29.942429	2
CAACGCA	73965	0.0	29.854189	5
AACGCAG	76095	0.0	29.049591	6
ACGCAGA	87535	0.0	25.228842	7
GTGGTAT	14715	0.0	25.066383	1
TGGTATC	13905	0.0	25.06134	2
CGCAGAG	88945	0.0	24.838446	8
GCAGAGT	102060	0.0	21.530209	9
GTACATG	73000	0.0	21.007639	1
TACATGG	72395	0.0	20.849005	2
GAGTACT	60470	0.0	20.60781	12-13
ACATGGG	74165	0.0	19.773394	3
CAGAGTA	99305	0.0	19.39897	10-11
GTACTTT	66210	0.0	18.79273	14-15
AGAGTAC	93400	0.0	18.739855	10-11
CATGGGG	54065	0.0	17.80536	4
>>END_MODULE