FastQCFastQC Report
Fri 27 May 2016
SRR2139898_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139898_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7118503
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA114000.050.776791
GTATCAA202650.036.7851371
ATCAACG239200.030.4745043
TCAACGC249200.029.453774
CAACGCA254600.028.7924125
TATCAAC256400.028.7270282
AACGCAG259100.028.368956
ACGCAGA261450.028.113967
GTGGTAT52450.027.4900511
TGGTATC50600.026.9906022
CGCAGAG305800.024.1057198
GTACATG259950.021.5034581
GCAGAGT350050.020.8894569
TACATGG259500.020.888692
ACATGGG253800.020.7414113
GAGTACT206700.020.02972812-13
CAGAGTA343050.019.03628210-11
GTACTTT225450.018.33611514-15
AGAGTAC324800.018.29051410-11
CATGGGG187650.017.8449694
Produced by FastQC (version 0.11.5)