##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139898_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7118503
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.788824981881724	33.0	33.0	33.0	33.0	33.0
2	32.83088157720802	33.0	33.0	33.0	33.0	33.0
3	33.43768247340768	33.0	33.0	33.0	33.0	37.0
4	36.625133121388025	37.0	37.0	37.0	37.0	37.0
5	36.6736701522778	37.0	37.0	37.0	37.0	37.0
6	36.67644194291974	37.0	37.0	37.0	37.0	37.0
7	36.66881842994236	37.0	37.0	37.0	37.0	37.0
8	36.658068276433966	37.0	37.0	37.0	37.0	37.0
9	36.661331181570056	37.0	37.0	37.0	37.0	37.0
10-11	36.64513634397569	37.0	37.0	37.0	37.0	37.0
12-13	36.86725614922126	37.0	37.0	37.0	37.0	38.5
14-15	38.75493976753259	40.0	37.0	40.0	37.0	40.0
16-17	38.71303938482572	40.0	37.0	40.0	37.0	40.0
18-19	38.74301570147544	40.0	37.0	40.0	37.0	40.0
20-21	38.77468309882224	40.0	37.0	40.0	37.0	40.0
22-23	38.810399121037584	40.0	37.0	40.0	37.0	40.0
24-25	38.81085666645973	40.0	37.0	40.0	37.0	40.0
26-27	38.52551278721175	40.0	37.0	40.0	37.0	40.0
28-29	38.52636997312558	40.0	37.0	40.0	37.0	40.0
30-31	38.526430225790705	40.0	37.0	40.0	37.0	40.0
32-33	38.49543709529141	40.0	37.0	40.0	37.0	40.0
34-35	38.35935944537911	40.0	37.0	40.0	37.0	40.0
36-37	38.396327336883914	40.0	37.0	40.0	37.0	40.0
38-39	38.3389514897038	40.0	37.0	40.0	37.0	40.0
40-41	38.33423138911795	40.0	37.0	40.0	37.0	40.0
42-43	38.27963034081484	40.0	37.0	40.0	37.0	40.0
44-45	38.198263072472315	40.0	37.0	40.0	37.0	40.0
46-47	38.09224170896667	40.0	37.0	40.0	35.0	40.0
48-49	37.902677560804335	40.0	37.0	40.0	33.0	40.0
50-51	37.9566994206148	40.0	37.0	40.0	33.0	40.0
52-53	37.378747646504536	40.0	37.0	40.0	33.0	40.0
54-55	33.84053274143963	37.0	33.0	40.0	10.5	40.0
56-57	36.75471857893064	37.0	37.0	40.0	30.0	40.0
58-59	37.434182441279376	38.5	37.0	40.0	33.0	40.0
60-61	37.408584718678384	38.5	37.0	40.0	33.0	40.0
62-63	36.58369237371035	37.0	37.0	40.0	33.0	40.0
64-65	32.757601687668014	37.0	33.0	40.0	10.5	40.0
66-67	35.550044271753634	37.0	33.0	40.0	30.0	40.0
68-69	36.26020401478773	37.0	37.0	40.0	33.0	40.0
70-71	36.10259045462732	37.0	37.0	38.5	33.0	40.0
72-73	35.563303373330974	37.0	37.0	37.0	33.0	40.0
74-75	35.3487749285601	37.0	33.0	37.0	33.0	40.0
76-77	34.01879877688984	35.0	33.0	37.0	30.0	37.0
78-79	35.34878061550782	37.0	33.0	37.0	33.0	37.0
80-81	35.51373834423081	37.0	33.0	37.0	33.0	37.0
82-83	35.44168899179249	37.0	33.0	37.0	33.0	37.0
84-85	35.32595065148854	37.0	33.0	37.0	33.0	37.0
86-87	35.22856443929258	37.0	33.0	37.0	33.0	37.0
88-89	35.1437094276647	37.0	33.0	37.0	33.0	37.0
90-91	35.05185846033784	37.0	33.0	37.0	33.0	37.0
92-93	34.98606496053088	37.0	33.0	37.0	33.0	37.0
94-95	34.94063437968889	37.0	33.0	37.0	33.0	37.0
96-97	34.910452579875	37.0	33.0	37.0	33.0	37.0
98-99	34.87597623529004	37.0	33.0	37.0	33.0	37.0
100	34.81714951544514	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	8.0
20	28.0
21	199.0
22	708.0
23	2166.0
24	4963.0
25	10397.0
26	18804.0
27	30574.0
28	46042.0
29	65528.0
30	90118.0
31	122077.0
32	164925.0
33	226469.0
34	327576.0
35	537894.0
36	1133292.0
37	1802004.0
38	2425406.0
39	109323.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.85189884478245	1.2643137880723513E-4	24.892231297497812	29.255743426340935
2	15.036739801625018	23.480979956557885	38.94166107789378	22.540619163923324
3	17.175797082107007	24.18676200979187	32.52054255670364	26.116898351397484
4	13.02613665580625	23.01391341732244	37.20687760885356	26.753072318017757
5	17.590224671268466	36.35387937635493	31.82649550193818	14.229400450438426
6	28.30342278425675	37.01413063954598	20.159884739811165	14.522561836386105
7	26.74264518818072	33.04123071943638	21.713891249325876	18.50223284305703
8	24.18154491190072	32.56556891245252	21.82922448722716	21.4236616884196
9	23.946537635792247	20.88398361284669	23.41488090965193	31.75459784170914
10-11	24.967749539474802	26.279528153601962	27.99928580489465	20.753436502028585
12-13	25.304084299746734	24.19260060717822	28.19137675435411	22.311938338720935
14-15	22.494146592338303	26.633268258789805	26.605959146185654	24.26662600268624
16-17	21.378857421206217	29.016109778706966	27.547465214769538	22.05756758531728
18-19	21.453569662048327	27.852386941467888	29.607095761566722	21.08694763491706
20-21	22.402330667529792	27.05067808286918	29.54318893938781	21.003802310213217
22-23	22.20482693563072	26.850618836910723	29.347005199689765	21.597549027768792
24-25	21.76781460558972	27.127728119151843	29.591581203505495	21.51287607175294
26-27	21.739338642476664	27.353261958757454	29.316238595010812	21.591160803755077
28-29	21.642104643734292	27.302615741412044	29.44690540190878	21.608374212944884
30-31	21.888751081061773	27.197301647272496	29.388857126228135	21.5250901454376
32-33	21.699330114516425	27.447288691660944	29.285188500074554	21.568192693748074
34-35	21.88770591077475	27.355478647792424	29.194399236829284	21.56241620460354
36-37	21.81065207659009	27.397796506490373	29.20868289908542	21.58286851783411
38-39	21.650615924319087	27.598750739846622	29.196762329105336	21.553871006728954
40-41	21.97005169418013	27.313009478140245	28.996799918290282	21.72013890938934
42-43	21.84967710718468	27.397781342615957	28.9286811158559	21.823860434343466
44-45	22.000120486022528	27.74321948862873	28.49899213296289	21.75766789238585
46-47	22.06292858794214	27.63067294535013	28.192643446575644	22.113755020132082
48-49	22.011482739280417	27.83353707693777	28.141140981530015	22.0138392022518
50-51	22.002872517103437	28.048497369007542	27.945150600319508	22.00347951356952
52-53	22.406959659453506	27.75072877936588	27.70912748202284	22.13318407915778
54-55	22.911636547333003	27.701847591714234	27.43941793721704	21.94709792373572
56-57	22.141360951274656	28.022054429806854	27.7866139982128	22.049970620705686
58-59	22.171712027906356	27.874598063539374	27.840762161794448	22.11292774675982
60-61	22.14633224863343	27.88733313404818	27.91995121848284	22.046383398835545
62-63	22.227376428627927	27.981729540323514	27.711470537092612	22.079423493955947
64-65	22.55498085243124	27.844726812225716	27.448758358953395	22.151533976389643
66-67	22.16039892602239	27.935277727087694	27.710027640630962	22.194295706258956
68-69	22.17964619904001	27.97447466038743	27.715985422137006	22.129893718435557
70-71	22.220097997909114	27.663708285172973	27.73813461066053	22.378059106257382
72-73	22.20014221671478	27.761428471144107	27.71612259339658	22.322306718744535
74-75	22.073398314891186	27.91477225727712	27.751589783373966	22.260239644457723
76-77	22.270772530843825	27.766592453456717	27.56426477246283	22.398370243236627
78-79	22.088580674957335	27.854255118810123	27.68915052551287	22.368013680719677
80-81	22.173512606161125	27.8679258615787	27.653548250547562	22.305013281712615
82-83	22.27519223479593	27.706077734217814	27.755478351781292	22.263251679204966
84-85	22.381678619397817	27.708500835768373	27.72421021383384	22.185610330999975
86-87	22.167485140631573	27.81619848415272	27.70917916903082	22.307137206184887
88-89	22.299203471898107	27.67232855622922	27.69999579726911	22.32847217460356
90-91	22.36325841876674	27.627308425683793	27.76597178291117	22.243461372638297
92-93	22.284318391558468	27.669946848645605	27.8058633358678	22.239871423928122
94-95	22.269342348112495	27.65992993098159	27.79683149378087	22.273896227125036
96-97	22.3061398293056	27.89434064591575	27.91440517064026	21.88511435413839
98-99	23.343023622464194	29.958524839804813	27.45961230018013	19.23883923755086
100	22.452828076191633	30.41627413377521	21.81363258068317	25.317265209349987
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1016.0
1	945.5
2	1475.5
3	2312.0
4	3317.5
5	5606.0
6	7548.5
7	9035.0
8	10427.5
9	10841.5
10	10419.5
11	9660.0
12	10333.5
13	13226.5
14	20739.0
15	32913.5
16	44659.5
17	52414.5
18	54147.0
19	50659.5
20	44800.5
21	38119.5
22	33079.5
23	32073.0
24	35601.0
25	43112.5
26	53853.5
27	66863.0
28	80971.5
29	96866.5
30	113410.5
31	129744.5
32	146765.5
33	164046.0
34	181407.5
35	197519.5
36	214234.0
37	231224.5
38	243921.0
39	253259.5
40	263529.5
41	272054.0
42	276692.0
43	282005.0
44	287490.0
45	292567.0
46	295477.0
47	295947.0
48	295138.0
49	290093.5
50	282953.5
51	273768.0
52	261134.0
53	248166.0
54	234176.5
55	216655.0
56	197571.5
57	178027.0
58	157896.5
59	136517.0
60	114356.5
61	93441.5
62	74078.0
63	57655.5
64	44380.0
65	33752.0
66	25489.0
67	19335.5
68	14824.0
69	10673.5
70	7350.0
71	5285.0
72	3799.5
73	2664.0
74	1853.5
75	1300.5
76	900.5
77	606.0
78	408.0
79	287.5
80	206.0
81	139.0
82	96.0
83	66.0
84	48.0
85	37.5
86	29.5
87	17.5
88	10.0
89	8.0
90	6.5
91	4.5
92	3.5
93	2.5
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.3881425631203638E-4
2	9.131133329577861E-4
3	0.0049589077928322844
4	0.012825730353699367
5	0.01394956214810895
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	7.023948715059894E-6
18-19	0.0
20-21	0.0
22-23	1.5454127618359475E-4
24-25	0.004728537341146966
26-27	0.040684529152489525
28-29	0.08308852437340745
30-31	0.07560478902616695
32-33	0.030116614579291566
34-35	7.356080929056046E-4
36-37	0.0
38-39	7.138792110663842E-6
40-41	0.0
42-43	2.892623571251863E-5
44-45	4.376003745859206E-5
46-47	1.0311104433443477E-4
48-49	3.9398121586766034E-4
50-51	0.011224427711538502
52-53	0.24025943096959088
54-55	0.9856196121267412
56-57	4.67369559837478E-4
58-59	0.0014439119729489655
60-61	0.045351964556509625
62-63	1.0066661781410302
64-65	4.166039104207854
66-67	1.5951423449198622E-5
68-69	4.827211182524337E-5
70-71	8.036809561952477E-4
72-73	0.012113411012048872
74-75	0.027493895867695538
76-77	0.00409222764287271
78-79	0.0
80-81	2.6328942769796287E-4
82-83	0.00756521070098196
84-85	0.039790494471814965
86-87	0.03899003216677654
88-89	0.012013942897322456
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	131.0
20-21	356.0
22-23	849.0
24-25	2802.0
26-27	3869.0
28-29	6157.0
30-31	13080.0
32-33	16906.0
34-35	24569.0
36-37	35186.0
38-39	42453.0
40-41	45025.0
42-43	55440.0
44-45	66282.0
46-47	64974.0
48-49	55068.0
50-51	49433.0
52-53	49677.0
54-55	47888.0
56-57	50105.0
58-59	51300.0
60-61	51093.0
62-63	52754.0
64-65	50666.0
66-67	53998.0
68-69	55448.0
70-71	55161.0
72-73	52406.0
74-75	53274.0
76-77	53237.0
78-79	57257.0
80-81	58119.0
82-83	56473.0
84-85	57868.0
86-87	61157.0
88-89	66449.0
90-91	70016.0
92-93	75536.0
94-95	98298.0
96-97	336229.0
98-99	1270236.0
100-101	3751278.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.08939466063691
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.17763151540211	75.88070682914903
2	7.716950689595202	13.132613256072808
3	1.8455142177852466	4.711010628868364
4	0.626520019960063	2.1324083696468774
5	0.26272830815466336	1.1177696350546793
6	0.13620312043548308	0.6953664641247049
7	0.0716265897133015	0.42662642122174393
8	0.04637442969210731	0.31567777201869424
9	0.02937845245923456	0.2249815262240312
>10	0.08508400109716474	1.0985473413889444
>50	0.0012757245892843672	0.07182260498704819
>100	6.107670487266447E-4	0.11060209690098909
>500	7.662306243681046E-5	0.050550253064133126
>1k	2.554100497301086E-5	0.03131680127788226
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.4047897430119788E-5	0.0
4	0.0	0.0	0.0	1.4047897430119788E-5	0.0
5	0.0	0.0	0.0	1.4047897430119788E-5	0.0
6	0.0	0.0	0.0	1.4047897430119788E-5	0.0
7	0.0	1.4047897430119788E-5	0.0	1.4047897430119788E-5	0.0
8	0.0	2.8095794860239576E-5	0.0	1.4047897430119788E-5	1.4047897430119788E-5
9	0.0	5.619158972047915E-5	0.0	2.8095794860239576E-5	2.8095794860239576E-5
10-11	0.0	5.619158972047915E-5	0.0	2.8095794860239576E-5	2.8095794860239576E-5
12-13	0.0	7.726343586565883E-5	0.0	5.619158972047915E-5	2.8095794860239576E-5
14-15	0.0	1.8262266659155725E-4	0.0	8.428738458071873E-5	4.916764100541926E-5
16-17	0.0	2.177424101668567E-4	0.0	1.123831794409583E-4	7.023948715059894E-5
18-19	0.0	2.247663588819166E-4	0.0	1.33455025586138E-4	8.428738458071873E-5
20-21	0.0	2.247663588819166E-4	0.0	1.8262266659155725E-4	9.131133329577862E-5
22-23	0.0	2.528621537421562E-4	0.0	2.0369451273673692E-4	1.1238317944095829E-4
24-25	0.0	2.598861024572161E-4	0.0	2.598861024572161E-4	1.264310768710781E-4
26-27	0.0	2.66910051172276E-4	0.0	4.2143692290359363E-4	1.264310768710781E-4
28-29	0.0	2.66910051172276E-4	0.0	0.001194071281560182	1.264310768710781E-4
30-31	0.0	3.30125589607815E-4	0.0	0.00330125589607815	1.4750292301625776E-4
32-33	0.0	3.371495383228749E-4	0.0	0.006735966817742439	1.6857476916143745E-4
34-35	0.0	4.0036507675841394E-4	0.0	0.012003928354037359	1.9667056402167702E-4
36-37	0.0	4.495327177638332E-4	0.0	0.019898846709764682	2.1071846145179682E-4
38-39	0.0	4.8465246133913266E-4	0.0	0.028685806552304606	2.1071846145179682E-4
40-41	0.0	5.478679997746717E-4	0.0	0.037114545010376476	2.247663588819166E-4
42-43	0.0	6.391793330704504E-4	0.0	0.0451850620839803	2.458382050270963E-4
44-45	0.0	7.30490666366229E-4	0.0	0.052054483927308874	2.66910051172276E-4
46-47	0.0	7.30490666366229E-4	0.0	0.05841115751443808	2.66910051172276E-4
48-49	0.0	7.445385637963488E-4	0.0	0.0642550828453679	2.9500584603251557E-4
50-51	0.0	7.726343586565883E-4	0.0	0.07060473248378205	2.9500584603251557E-4
52-53	0.0	7.726343586565883E-4	0.0	0.07664532837873356	3.090537434626353E-4
54-55	0.0	7.866822560867081E-4	0.0	0.0815972122228508	3.231016408927551E-4
56-57	0.0	7.866822560867081E-4	0.0	0.08609956334920418	3.231016408927551E-4
58-59	0.0	7.937062048017679E-4	0.0	0.09062298632170275	3.371495383228749E-4
60-61	0.0	8.428738458071873E-4	0.0	0.09514640929420132	3.511974357529947E-4
62-63	0.0	8.850175380975467E-4	0.0	0.09940994616424267	3.511974357529947E-4
64-65	0.0	9.412091278180258E-4	0.0	0.10381396200858523	3.582213844680546E-4
66-67	0.0	9.693049226782653E-4	0.0	0.10921537857046629	3.722692818981744E-4
68-69	0.0	0.0010044246662535648	0.0	0.11428666954273953	3.792932306132343E-4
70-71	0.0	0.0010395444098288643	0.0	0.11859235010507124	3.9334112804335404E-4
72-73	0.0	0.001053592307258984	0.0	0.12195682153958494	3.9334112804335404E-4
74-75	0.0	0.0010676402046891037	0.0	0.12504033502549622	3.9334112804335404E-4
76-77	0.0	0.0010957359995493435	0.0	0.12782181871665993	3.9334112804335404E-4
78-79	0.0	0.0011097838969794632	0.0	0.12925470425453217	3.9334112804335404E-4
80-81	0.0	0.0011168078456945231	0.0	0.12978150040816167	4.1441297418853373E-4
82-83	0.0	0.001123831794409583	0.0	0.13023805707464056	4.354848203337134E-4
84-85	0.0	0.001123831794409583	0.0	0.13048389527966764	4.354848203337134E-4
86-87	0.0	0.0011659754866999424	0.0	0.13056818266424836	4.354848203337134E-4
88	0.0	0.0012081191789903016	0.0	0.13057520661296343	4.354848203337134E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11400	0.0	50.77679	1
GTATCAA	20265	0.0	36.785137	1
ATCAACG	23920	0.0	30.474504	3
TCAACGC	24920	0.0	29.45377	4
CAACGCA	25460	0.0	28.792412	5
TATCAAC	25640	0.0	28.727028	2
AACGCAG	25910	0.0	28.36895	6
ACGCAGA	26145	0.0	28.11396	7
GTGGTAT	5245	0.0	27.490051	1
TGGTATC	5060	0.0	26.990602	2
CGCAGAG	30580	0.0	24.105719	8
GTACATG	25995	0.0	21.503458	1
GCAGAGT	35005	0.0	20.889456	9
TACATGG	25950	0.0	20.88869	2
ACATGGG	25380	0.0	20.741411	3
GAGTACT	20670	0.0	20.029728	12-13
CAGAGTA	34305	0.0	19.036282	10-11
GTACTTT	22545	0.0	18.336115	14-15
AGAGTAC	32480	0.0	18.290514	10-11
CATGGGG	18765	0.0	17.844969	4
>>END_MODULE