FastQCFastQC Report
Fri 27 May 2016
SRR2139897_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139897_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14319236
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA229800.047.384031
GTATCAA402450.036.0011941
TCAACGC487050.029.3012454
ATCAACG486450.029.2509383
CAACGCA502450.028.400395
TATCAAC505850.028.3898932
AACGCAG517700.027.617566
ACGCAGA583800.024.4250167
CGCAGAG589250.024.1991088
GTGGTAT119250.023.3719351
TGGTATC116750.022.9612432
GCAGAGT677450.020.9166589
GAGTACT411150.020.64132312-13
GTACATG457650.020.5573441
TACATGG452700.020.1147672
ACATGGG464650.019.1120933
CAGAGTA653550.019.0356510-11
AGAGTAC610700.018.80400510-11
GTACTTT459500.018.46076814-15
CATGGGG358850.017.7384324
Produced by FastQC (version 0.11.5)