FastQCFastQC Report
Fri 27 May 2016
SRR2139896_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139896_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6372904
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70750.047.2438131
GTATCAA124150.034.672151
TCAACGC144850.029.0353954
ATCAACG145900.028.7143973
CAACGCA147550.028.4454485
AACGCAG154750.027.2239636
TATCAAC157950.026.8361282
ACGCAGA179050.023.460367
CGCAGAG181850.023.0539328
TACATGG170400.023.0417042
GTACATG176950.022.9322871
ACATGGG175300.021.8816743
CATGGGG126100.019.6595154
GCAGAGT223250.018.8155779
GAGTACT139550.017.68647612-13
AGAGTAC199450.017.45450210-11
GTTATCG3100.001439968517.31642594
CAGAGTA218400.017.09741410-11
AGTACTT153200.015.57405112-13
GAGTACA121650.015.4786891
Produced by FastQC (version 0.11.5)