##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139896_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6372904
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.74289586034875	33.0	33.0	33.0	33.0	33.0
2	32.75720864459907	33.0	33.0	33.0	33.0	33.0
3	32.66780434790795	33.0	33.0	33.0	33.0	33.0
4	36.65219184221196	37.0	37.0	37.0	37.0	37.0
5	36.70545280456131	37.0	37.0	37.0	37.0	37.0
6	36.71300352241302	37.0	37.0	37.0	37.0	37.0
7	36.69983056389991	37.0	37.0	37.0	37.0	37.0
8	36.70284253458078	37.0	37.0	37.0	37.0	37.0
9	36.704722054498234	37.0	37.0	37.0	37.0	37.0
10-11	36.67398096691869	37.0	37.0	37.0	37.0	37.0
12-13	36.649852563289826	37.0	37.0	37.0	37.0	37.0
14-15	38.85008349411822	40.0	37.0	40.0	37.0	40.0
16-17	38.80993029237534	40.0	37.0	40.0	37.0	40.0
18-19	38.82158290788627	40.0	37.0	40.0	37.0	40.0
20-21	38.8415973511888	40.0	37.0	40.0	37.0	40.0
22-23	38.87677185016227	40.0	37.0	40.0	37.0	40.0
24-25	38.896622872950616	40.0	40.0	40.0	37.0	40.0
26-27	38.63242443256084	40.0	37.0	40.0	37.0	40.0
28-29	38.604635861530014	40.0	37.0	40.0	37.0	40.0
30-31	38.61685222432743	40.0	37.0	40.0	37.0	40.0
32-33	38.615210658623795	40.0	37.0	40.0	37.0	40.0
34-35	38.463731631681526	40.0	37.0	40.0	37.0	40.0
36-37	38.52349210212082	40.0	37.0	40.0	37.0	40.0
38-39	38.466017441717085	40.0	37.0	40.0	37.0	40.0
40-41	38.471886926627995	40.0	37.0	40.0	37.0	40.0
42-43	38.429320534668584	40.0	37.0	40.0	37.0	40.0
44-45	38.36124267260519	40.0	37.0	40.0	37.0	40.0
46-47	38.29077757655726	40.0	37.0	40.0	37.0	40.0
48-49	38.06973814077098	40.0	37.0	40.0	37.0	40.0
50-51	38.16388533538922	40.0	37.0	40.0	37.0	40.0
52-53	38.129561634271	40.0	37.0	40.0	37.0	40.0
54-55	33.90734219699325	40.0	37.0	40.0	10.5	40.0
56-57	36.908331178267495	38.5	37.0	40.0	30.0	40.0
58-59	37.6255441860521	38.5	37.0	40.0	33.0	40.0
60-61	37.654606628115324	40.0	37.0	40.0	33.0	40.0
62-63	37.42903205271126	38.5	37.0	40.0	33.0	40.0
64-65	32.87310534279801	37.0	33.0	40.0	10.5	40.0
66-67	35.72720015812309	37.0	35.0	40.0	30.0	40.0
68-69	36.508791334722304	37.0	37.0	40.0	33.0	40.0
70-71	36.42387793201786	37.0	37.0	40.0	33.0	40.0
72-73	35.78429349380532	37.0	37.0	37.0	33.0	40.0
74-75	35.838473632147526	37.0	37.0	37.0	33.0	40.0
76-77	34.02177384927427	35.0	33.0	37.0	30.0	37.0
78-79	35.52228880548026	37.0	33.0	37.0	33.0	37.0
80-81	35.706721025700716	37.0	37.0	37.0	33.0	37.0
82-83	35.63093557795092	37.0	37.0	37.0	33.0	37.0
84-85	35.50701164308789	37.0	35.0	37.0	33.0	37.0
86-87	35.399508313799366	37.0	33.0	37.0	33.0	37.0
88-89	35.315001816405484	37.0	33.0	37.0	33.0	37.0
90-91	35.21915868444218	37.0	33.0	37.0	33.0	37.0
92-93	35.152821038284706	37.0	33.0	37.0	33.0	37.0
94-95	35.101432834690684	37.0	33.0	37.0	33.0	37.0
96-97	35.06714676712441	37.0	33.0	37.0	33.0	37.0
98-99	35.02766650425172	37.0	33.0	37.0	33.0	37.0
100	34.9139115648928	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	4.0
20	27.0
21	136.0
22	424.0
23	1360.0
24	3383.0
25	7085.0
26	12759.0
27	21339.0
28	32549.0
29	47786.0
30	66812.0
31	92567.0
32	127771.0
33	179462.0
34	265901.0
35	445913.0
36	967832.0
37	1601321.0
38	2380389.0
39	118081.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.40084269275044	20.260480936163482	12.919055425909445	24.419620945176643
2	14.639793726690375	21.845143124704215	40.994246892782314	22.520816255823092
3	17.937027487647732	26.961906514913498	30.83121375074399	24.269852246694775
4	11.811896280307511	18.129414683699572	40.09408889654035	29.964600139452568
5	12.520386703914122	38.49704949935654	36.37363068520669	12.608933111522646
6	28.910211106271177	39.032880457637525	19.300855622491724	12.756052813599577
7	25.465188240714127	33.00493778032746	24.37976470381478	17.15010927514364
8	24.107910616572916	36.792583098694095	22.12068469884373	16.978821585889257
9	25.65405033560838	15.925283042079405	21.54692742900254	36.873739193309675
10-11	23.64313819884938	26.855425721146908	30.023039731965206	19.47839634803851
12-13	24.494963363640814	24.630670727191244	29.810405115156293	21.063960794011646
14-15	21.63060199871205	25.902069135201156	27.324254374457862	25.143074491628937
16-17	21.226257291809198	28.953629303061838	27.990771554067027	21.829341851061933
18-19	21.21491238531131	28.272715233118213	29.604965020656206	20.90740736091427
20-21	22.067397848696334	27.557806566892218	29.369946885488275	21.00484869892317
22-23	21.70114576003505	27.618643567756017	29.1969746235463	21.483236048662633
24-25	21.477355672345357	27.655215976769487	29.378630699614227	21.48879765127093
26-27	21.540237812279695	27.801204320172907	29.128285770537087	21.530272097010307
28-29	21.37352735293107	27.957317952914135	29.238419377617298	21.430735316537497
30-31	21.723152104473307	27.78905085437723	29.07164836023331	21.41614868091616
32-33	21.48950776132472	27.956809972943475	29.08368103266029	21.470001233071514
34-35	21.627356719591436	28.038519555382496	29.03482338642708	21.299300338598986
36-37	21.593365190741928	27.899681253384728	29.034887103307618	21.472066452565727
38-39	21.47800869543378	28.042296497713963	29.106056031088066	21.373638775764192
40-41	21.692922495176802	27.931447195391613	28.93462521661797	21.44100509281361
42-43	21.599689354073977	27.986905320595284	28.805404429186478	21.60800089614426
44-45	21.654831439688767	28.198792545337298	28.600578970410734	21.5457970445632
46-47	21.724884085532103	28.129072867916776	28.311004736756868	21.83503830979425
48-49	21.729234926110465	28.238645797949065	28.24568957289893	21.78642970304154
50-51	21.669935739394763	28.315436878128164	28.222328701485385	21.792298680991685
52-53	21.837578025312965	28.25528502948581	28.11564989481671	21.79148705038452
54-55	22.745524237044137	28.092815516419	27.51667428137931	21.644985965157552
56-57	21.867420554070975	28.30836405529548	28.031677143617166	21.79253824701637
58-59	21.77252502365021	28.395910409404113	28.069587341645057	21.761977225300615
60-61	21.86097217686431	28.35749595019323	28.000529652434942	21.781002220507524
62-63	21.839169381774536	28.39553826122302	27.968371807196064	21.796920549806384
64-65	22.27021707777566	28.30547610875211	27.61978709761554	21.804519715856696
66-67	21.856383058922162	28.33416879316478	27.87606814299523	21.933380004917826
68-69	21.81909659528166	28.354874162109684	27.944159450888524	21.881869791720135
70-71	21.841900820065145	28.129845395367102	27.976603883656175	22.051649900911574
72-73	21.89608003061837	28.20871728471844	27.90178123606239	21.9934214486008
74-75	21.745353766149446	28.340085355233217	27.911163212815303	22.003397665802034
76-77	21.841990418730568	28.26886728487141	27.849313210859428	22.039829085538596
78-79	21.858310694676238	28.27355979474284	27.810289300833453	22.05784020974747
80-81	21.8496800897951	28.262018227759096	27.86617418107938	22.022127501366427
82-83	21.938673670884494	28.248812881862346	27.92915831014412	21.883355137109035
84-85	22.045615017423014	28.201056080219093	27.816572117439698	21.93675678491819
86-87	21.817955182022907	28.255823083964017	27.900607597436846	22.02561413657623
88-89	21.90262224136571	28.218373494135797	27.874523286994368	22.004480977504127
90-91	21.968636869353155	28.22900269360733	27.9139053172911	21.88845511974841
92-93	21.94995760007235	28.230520608199765	27.91927106201661	21.900250729711274
94-95	21.80157888770651	28.31471805782659	27.956953825287716	21.926749229179187
96-97	21.883064591898115	28.373870376998696	28.03619185654928	21.706873174553905
98-99	22.954091987775268	30.336199772700063	28.207115292889124	18.50259294663555
100	29.042737276802548	41.58878265489589	29.368480068301565	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1185.0
1	1200.0
2	1930.5
3	2890.0
4	4073.0
5	6823.0
6	9077.0
7	10224.5
8	10664.5
9	10104.0
10	9196.0
11	8364.0
12	9058.0
13	11773.5
14	17676.0
15	25707.5
16	32207.5
17	35901.0
18	36832.0
19	35785.5
20	33995.0
21	32603.0
22	33152.0
23	37421.5
24	45415.5
25	56821.0
26	70962.0
27	86191.0
28	99952.5
29	112814.5
30	125109.0
31	137536.0
32	152296.5
33	167730.0
34	182319.5
35	196527.0
36	211443.0
37	224749.5
38	234456.0
39	242154.5
40	249958.0
41	256650.0
42	259792.0
43	263544.5
44	268502.5
45	270581.0
46	270794.5
47	270385.0
48	268964.0
49	263216.5
50	255150.5
51	246551.0
52	234051.5
53	220300.0
54	205795.0
55	188638.0
56	170043.5
57	150499.0
58	131334.0
59	112373.5
60	93499.0
61	75923.0
62	59710.0
63	45858.0
64	34624.5
65	26058.0
66	19776.0
67	15142.5
68	11497.0
69	8384.5
70	6054.5
71	4371.5
72	3085.5
73	2088.5
74	1415.0
75	965.5
76	660.0
77	434.0
78	272.5
79	174.5
80	121.5
81	88.0
82	65.0
83	41.0
84	27.0
85	23.5
86	20.0
87	14.5
88	10.0
89	7.5
90	4.5
91	2.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0029342980845153167
4	0.013259261397943544
5	0.01732334270216529
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0015381298867504784
26-27	0.03466772407412016
28-29	0.0801338140419425
30-31	0.07897424759638462
32-33	0.03526666578809082
34-35	8.871233725642522E-4
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	1.1972410780221205
56-57	8.802426371224374E-5
58-59	0.0
60-61	0.0
62-63	0.0
64-65	4.861624100204603
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.009287661443040258
74-75	0.031711372681179004
76-77	0.005009820925528914
78-79	0.0
80-81	0.0
82-83	0.003867134664984907
84-85	0.03908460642163707
86-87	0.04335181068472807
88-89	0.014192808184568926
90-91	3.2292276968276176E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	113.0
20-21	304.0
22-23	672.0
24-25	1775.0
26-27	4108.0
28-29	7935.0
30-31	17073.0
32-33	21810.0
34-35	29512.0
36-37	45157.0
38-39	53200.0
40-41	53075.0
42-43	60050.0
44-45	70654.0
46-47	70873.0
48-49	64457.0
50-51	58793.0
52-53	58079.0
54-55	59274.0
56-57	63209.0
58-59	62497.0
60-61	61870.0
62-63	61711.0
64-65	58508.0
66-67	63533.0
68-69	63724.0
70-71	62353.0
72-73	56983.0
74-75	57330.0
76-77	58157.0
78-79	61519.0
80-81	62433.0
82-83	58678.0
84-85	57269.0
86-87	60407.0
88-89	64031.0
90-91	67176.0
92-93	68201.0
94-95	78300.0
96-97	197061.0
98-99	1320507.0
100-101	2930533.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.67557733242506
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.25123553937333	78.22577945339862
2	7.028398142604832	12.183809334648355
3	1.6145427721288952	4.19824280706498
4	0.5689758270522317	1.9726523319178482
5	0.2341544652327759	1.0147736729508048
6	0.11080932828236609	0.5762677501615378
7	0.062402781253614946	0.3786157964614275
8	0.03396969817320769	0.2355474560776801
9	0.023145646509730573	0.18055460465668136
>10	0.07104018597674397	0.8926160019390048
>50	8.892111732854845E-4	0.04974439754126137
>100	4.1015821825657323E-4	0.07426778436705583
>500	2.6244020703958707E-5	0.01712860881467922
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.569143360703378E-5
2	0.0	4.7074300821101336E-5	0.0	3.138286721406756E-5	1.569143360703378E-5
3	0.0	4.7074300821101336E-5	0.0	3.138286721406756E-5	1.569143360703378E-5
4	0.0	6.276573442813511E-5	0.0	6.276573442813511E-5	1.569143360703378E-5
5	0.0	7.84571680351689E-5	0.0	9.414860164220267E-5	1.569143360703378E-5
6	0.0	7.84571680351689E-5	0.0	9.414860164220267E-5	1.569143360703378E-5
7	0.0	7.84571680351689E-5	0.0	1.0984003524923645E-4	1.569143360703378E-5
8	0.0	7.84571680351689E-5	0.0	1.0984003524923645E-4	1.569143360703378E-5
9	0.0	2.196800704984729E-4	0.0	1.4122290246330402E-4	1.569143360703378E-5
10-11	0.0	2.196800704984729E-4	0.0	1.4122290246330402E-4	1.569143360703378E-5
12-13	0.0	2.196800704984729E-4	0.0	1.490686192668209E-4	1.569143360703378E-5
14-15	0.0	2.589086545160573E-4	0.0	1.8045148648088846E-4	1.569143360703378E-5
16-17	0.0	2.667543713195742E-4	0.0	2.0398863689143914E-4	1.569143360703378E-5
18-19	0.0	3.0598295533715866E-4	0.0	2.196800704984729E-4	1.569143360703378E-5
20-21	0.0	3.609029729617769E-4	0.0	2.353715041055067E-4	1.569143360703378E-5
22-23	0.0	3.765944065688107E-4	0.0	2.981372385336418E-4	1.569143360703378E-5
24-25	1.569143360703378E-5	4.472058578004627E-4	0.0	3.216743889441925E-4	1.569143360703378E-5
26-27	1.569143360703378E-5	4.8643444181804715E-4	0.0	4.0013155697936136E-4	1.569143360703378E-5
28-29	1.569143360703378E-5	4.8643444181804715E-4	0.0	7.76725963548172E-4	1.569143360703378E-5
30-31	1.569143360703378E-5	5.648916098532161E-4	0.0	0.002071269236128459	1.569143360703378E-5
32-33	1.569143360703378E-5	5.648916098532161E-4	0.0	0.0045897443300573805	1.569143360703378E-5
34-35	1.569143360703378E-5	6.198116274778343E-4	0.0	0.007994785422783712	1.569143360703378E-5
36-37	1.569143360703378E-5	6.982687955130032E-4	0.0	0.013353409999585748	1.569143360703378E-5
38-39	1.569143360703378E-5	7.531888131376214E-4	0.0	0.020744075228498655	1.569143360703378E-5
40-41	3.138286721406756E-5	7.84571680351689E-4	0.0	0.02774245461723572	1.569143360703378E-5
42-43	3.138286721406756E-5	8.081088307622397E-4	0.0	0.03402687377685275	1.569143360703378E-5
44-45	3.922858401758445E-5	8.630288483868579E-4	0.0	0.040609430175003416	1.569143360703378E-5
46-47	5.4920017624618225E-5	9.101031492079592E-4	0.0	0.047505815245294764	1.569143360703378E-5
48-49	6.276573442813511E-5	9.101031492079592E-4	0.0	0.05423744026271226	1.569143360703378E-5
50-51	7.84571680351689E-5	0.0010042517508501618	0.0	0.060969065280129756	2.3537150410550668E-5
52-53	7.84571680351689E-5	0.0010042517508501618	0.0	0.06760654169590503	3.922858401758445E-5
54-55	7.84571680351689E-5	0.0010042517508501618	0.0	0.07371835508584469	4.7074300821101336E-5
56-57	7.84571680351689E-5	0.0010199431844571955	0.0	0.08004984854628283	4.7074300821101336E-5
58-59	7.84571680351689E-5	0.0010356346180642295	0.0	0.08662455922762999	4.7074300821101336E-5
60-61	7.84571680351689E-5	0.0010591717684747801	0.0	0.09266576116633798	4.7074300821101336E-5
62-63	7.84571680351689E-5	0.0011219375029029152	0.0	0.09895802604275852	4.7074300821101336E-5
64-65	8.630288483868579E-5	0.0012239318213486349	0.0	0.10546212527287403	4.7074300821101336E-5
66-67	9.414860164220267E-5	0.0012239318213486349	0.0	0.11293909338662562	4.7074300821101336E-5
68-69	9.414860164220267E-5	0.00128669755577677	0.0	0.12039252434996667	4.7074300821101336E-5
70-71	1.0984003524923645E-4	0.0013416175734013882	0.0	0.12663771492556614	4.7074300821101336E-5
72-73	1.0984003524923645E-4	0.001349463290204905	0.0	0.1311882306716059	9.414860164220267E-5
74-75	1.0984003524923645E-4	0.0013651547238119387	0.0	0.1349463290204905	9.414860164220267E-5
76-77	1.0984003524923645E-4	0.0014436118918471077	0.0	0.13854751303330476	9.414860164220267E-5
78-79	1.1768575205275335E-4	0.0015063776262752428	0.0	0.14096399380878796	9.414860164220267E-5
80-81	1.2553146885627023E-4	0.0015063776262752428	0.0	0.14174856548913964	1.0984003524923645E-4
82-83	1.2553146885627023E-4	0.0015063776262752428	0.0	0.14225853708136824	1.2553146885627023E-4
84-85	1.2553146885627023E-4	0.0015063776262752428	0.0	0.14252529145268783	1.3337718565978714E-4
86-87	1.2553146885627023E-4	0.0015220690598822767	0.0	0.14267436007195464	1.4122290246330402E-4
88	1.2553146885627023E-4	0.0016476005287385468	0.0	0.1426978972223652	1.4122290246330402E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7075	0.0	47.243813	1
GTATCAA	12415	0.0	34.67215	1
TCAACGC	14485	0.0	29.035395	4
ATCAACG	14590	0.0	28.714397	3
CAACGCA	14755	0.0	28.445448	5
AACGCAG	15475	0.0	27.223963	6
TATCAAC	15795	0.0	26.836128	2
ACGCAGA	17905	0.0	23.46036	7
CGCAGAG	18185	0.0	23.053932	8
TACATGG	17040	0.0	23.041704	2
GTACATG	17695	0.0	22.932287	1
ACATGGG	17530	0.0	21.881674	3
CATGGGG	12610	0.0	19.659515	4
GCAGAGT	22325	0.0	18.815577	9
GAGTACT	13955	0.0	17.686476	12-13
AGAGTAC	19945	0.0	17.454502	10-11
GTTATCG	310	0.0014399685	17.316425	94
CAGAGTA	21840	0.0	17.097414	10-11
AGTACTT	15320	0.0	15.574051	12-13
GAGTACA	12165	0.0	15.478689	1
>>END_MODULE