FastQCFastQC Report
Fri 27 May 2016
SRR2139895_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139895_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4925507
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60650.039.5739941
GTATCAA97900.030.7194371
TCAACGC108600.026.9694564
ATCAACG108900.026.6680953
CAACGCA109800.026.634115
AACGCAG113300.025.9170286
TATCAAC118850.024.8525752
TACATGG118100.023.1564872
GTACATG122300.023.0705971
ACGGGCG2900.022.789215
ACGCAGA131350.022.2926487
CGCAGAG132950.022.0243668
ACATGGG120100.021.8421443
CATGGGG87200.020.3234084
CGTACGA2200.019.81470960-61
GGCGTCG3351.2732926E-1118.4923448
GCAGAGT162750.017.940899
GAGTACT104250.017.41118612-13
AGAGTAC141700.017.22515710-11
CAGAGTA155500.016.73230210-11
Produced by FastQC (version 0.11.5)