##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139895_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4925507
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.729641638921635	33.0	33.0	33.0	33.0	33.0
2	32.74490950880793	33.0	33.0	33.0	33.0	33.0
3	32.6502073796667	33.0	33.0	33.0	33.0	33.0
4	36.633488694666354	37.0	37.0	37.0	37.0	37.0
5	36.6911609302352	37.0	37.0	37.0	37.0	37.0
6	36.70027552493581	37.0	37.0	37.0	37.0	37.0
7	36.68708216230329	37.0	37.0	37.0	37.0	37.0
8	36.68899627997686	37.0	37.0	37.0	37.0	37.0
9	36.69153652608757	37.0	37.0	37.0	37.0	37.0
10-11	36.66245119537948	37.0	37.0	37.0	37.0	37.0
12-13	36.640347582492524	37.0	37.0	37.0	37.0	37.0
14-15	38.83121686762398	40.0	37.0	40.0	37.0	40.0
16-17	38.796373043424765	40.0	37.0	40.0	37.0	40.0
18-19	38.80997286167698	40.0	37.0	40.0	37.0	40.0
20-21	38.83078805247453	40.0	37.0	40.0	37.0	40.0
22-23	38.85893090669667	40.0	37.0	40.0	37.0	40.0
24-25	38.87515192782533	40.0	37.0	40.0	37.0	40.0
26-27	38.60182853299212	40.0	37.0	40.0	37.0	40.0
28-29	38.57398358551977	40.0	37.0	40.0	37.0	40.0
30-31	38.593137594590004	40.0	37.0	40.0	37.0	40.0
32-33	38.592158517510185	40.0	37.0	40.0	37.0	40.0
34-35	38.42515141025861	40.0	37.0	40.0	37.0	40.0
36-37	38.49370725570202	40.0	37.0	40.0	37.0	40.0
38-39	38.43795448836677	40.0	37.0	40.0	37.0	40.0
40-41	38.450591629792555	40.0	37.0	40.0	37.0	40.0
42-43	38.41493845425346	40.0	37.0	40.0	37.0	40.0
44-45	38.35304187207912	40.0	37.0	40.0	37.0	40.0
46-47	38.290704771549116	40.0	37.0	40.0	37.0	40.0
48-49	38.065463856577196	40.0	37.0	40.0	35.0	40.0
50-51	38.16985347247695	40.0	37.0	40.0	37.0	40.0
52-53	38.13947957847288	40.0	37.0	40.0	37.0	40.0
54-55	33.50639429348447	40.0	33.0	40.0	10.5	40.0
56-57	36.81971589882062	38.5	35.0	40.0	30.0	40.0
58-59	37.62377812677188	38.5	37.0	40.0	33.0	40.0
60-61	37.68086342148301	40.0	37.0	40.0	33.0	40.0
62-63	37.46498704521735	38.5	37.0	40.0	33.0	40.0
64-65	32.47901400714012	37.0	33.0	40.0	8.5	40.0
66-67	35.65261472617476	37.0	33.0	40.0	30.0	40.0
68-69	36.5460313546229	37.0	37.0	40.0	33.0	40.0
70-71	36.4874900554578	37.0	37.0	40.0	33.0	40.0
72-73	35.829932138279254	37.0	37.0	37.0	33.0	40.0
74-75	35.89662793840583	37.0	37.0	37.0	33.0	40.0
76-77	34.10691973251609	35.0	33.0	37.0	30.0	37.0
78-79	35.55344649327441	37.0	35.0	37.0	33.0	37.0
80-81	35.71748423908298	37.0	37.0	37.0	33.0	37.0
82-83	35.63516220309634	37.0	37.0	37.0	33.0	37.0
84-85	35.50411658394462	37.0	35.0	37.0	33.0	37.0
86-87	35.39334673557849	37.0	33.0	37.0	33.0	37.0
88-89	35.30718075434896	37.0	33.0	37.0	33.0	37.0
90-91	35.2151280649868	37.0	33.0	37.0	33.0	37.0
92-93	35.152363497890754	37.0	33.0	37.0	33.0	37.0
94-95	35.104874108743914	37.0	33.0	37.0	33.0	37.0
96-97	35.07182441781865	37.0	33.0	37.0	33.0	37.0
98-99	35.041135833635735	37.0	33.0	37.0	33.0	37.0
100	34.99839657796067	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	13.0
21	95.0
22	341.0
23	1059.0
24	2557.0
25	5463.0
26	10160.0
27	16827.0
28	26098.0
29	37938.0
30	53732.0
31	74034.0
32	102783.0
33	143819.0
34	211136.0
35	350646.0
36	764534.0
37	1197406.0
38	1835579.0
39	91282.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.27335378875718	20.363345336835376	12.999311542953853	24.363989331453595
2	14.51223193876285	21.980539262252595	41.15795592210102	22.349272876883536
3	17.91379596689608	27.007161741996182	30.818778325467207	24.26026396564053
4	11.756503877838936	18.201883162081405	40.40049072997912	29.641122230100542
5	12.442544163526682	38.42322259522094	36.59064640506578	12.543586836186599
6	28.76083619412174	39.262760158497386	19.37816756731845	12.598236080062417
7	25.371053172800284	33.10254152516685	24.513111036082176	17.013294265950694
8	23.78494234197617	37.126594277502804	22.215905895575826	16.872557484945204
9	25.41137389511374	16.25404247725158	21.851110961775102	36.48347266585957
10-11	23.42634981535911	27.08330330258388	30.168244609133637	19.322102272923374
12-13	24.314694913640363	24.756152006280775	30.0862428984468	20.842910181632064
14-15	21.393472793765188	26.177092023217103	27.554686248542538	24.87474893447517
16-17	21.063496610602726	29.21939812490369	28.22158206251661	21.495523201976972
18-19	20.937225345532955	28.583108297277825	29.853251655108803	20.626414702080414
20-21	21.752149255143483	27.888908944024042	29.698236314417503	20.660705486414972
22-23	21.43710315766219	27.802936246235575	29.516546906039693	21.24341369006254
24-25	21.194009572044397	27.855548889685906	29.68647756808495	21.263963970184744
26-27	21.27828586880517	28.095175042860493	29.388799334027528	21.237739754306805
28-29	21.1761899069338	28.214253193984145	29.4885708399358	21.12098605914625
30-31	21.45778528988813	28.05799708765336	29.379394643144813	21.1048229793137
32-33	21.184357001063784	28.25859473537642	29.353513161558958	21.203535102000846
34-35	21.371721340528207	28.322801083902462	29.249354932161854	21.056122643407484
36-37	21.36868731742759	28.17372147448447	29.28628002660784	21.171311181480103
38-39	21.183108752008433	28.32640961679804	29.329514529537636	21.160967101655896
40-41	21.429035939655737	28.245722659460082	29.169400710203096	21.15584069068108
42-43	21.296820846456313	28.342842188847122	29.00643240836425	21.353904556332314
44-45	21.45892796251794	28.486782006890937	28.77712398296712	21.277166047624007
46-47	21.472850782547106	28.434806698984932	28.54281071539439	21.549531803073574
48-49	21.460974268256297	28.552691679763427	28.51384531018325	21.472488741797026
50-51	21.464494900326184	28.625039004951358	28.440974157947075	21.46949193677538
52-53	21.551315357095667	28.56177467052313	28.342173428860967	21.54473654352024
54-55	22.612066067274032	28.37220888642651	27.66123973745998	21.354485308839482
56-57	21.535905970447953	28.62505251234359	28.29815785848285	21.54088365872561
58-59	21.5219032397328	28.693273274230037	28.239666903020264	21.5451565830169
60-61	21.549317494780105	28.641664396727904	28.321756549096776	21.48726155939521
62-63	21.589396032037307	28.569680350623834	28.317891385400245	21.523032231938615
64-65	22.047984792975704	28.53777545009264	27.899323477956678	21.51491627897498
66-67	21.627718001621833	28.669456996167497	28.107976873107095	21.594848129103575
68-69	21.569271044189758	28.633919661697792	28.21980445231944	21.577004841793006
70-71	21.544347569631856	28.41334293059025	28.24686065226319	21.795448847514702
72-73	21.713423075202467	28.45828951139891	28.15943909906688	21.668848314331747
74-75	21.48966632100978	28.613397911647592	28.184336417094038	21.712599350248592
76-77	21.570931861509607	28.570786032270203	28.107958741601358	21.750323364618836
78-79	21.59144350864033	28.594742443513894	28.079387213586326	21.734426834259445
80-81	21.54730433051538	28.546943948455894	28.138727237499563	21.767024483529166
82-83	21.62711606037589	28.54095978006902	28.211602095231587	21.620322064323506
84-85	21.762196925221033	28.50993230792809	28.113315807335347	21.61455495951553
86-87	21.553506191380418	28.532241535937903	28.184888176534894	21.729364096146785
88-89	21.67203646463228	28.560925959507678	28.130055915890285	21.63698165996976
90-91	21.681123772215148	28.57347464909768	28.181346628782894	21.564054949904275
92-93	21.682704182901606	28.490500431380973	28.18368764174522	21.643107743972205
94-95	21.538928697933972	28.573040027448464	28.236030044620854	21.65200122999671
96-97	21.628785924452647	28.677590640912076	28.308610387052845	21.385013047582436
98-99	22.69941411293413	30.62431834943908	28.468166456568937	18.20810108105786
100	21.585875653912538	31.581714729245043	22.440166529232062	24.39224308761036
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	930.0
1	878.0
2	1382.5
3	2122.0
4	3003.5
5	5028.0
6	6707.5
7	7565.0
8	7982.0
9	7719.5
10	6999.0
11	6173.0
12	6594.0
13	8715.5
14	13183.5
15	19161.0
16	23925.5
17	26715.5
18	27672.5
19	27041.5
20	25934.5
21	25202.0
22	25636.5
23	28737.5
24	34720.0
25	43290.0
26	54846.5
27	67487.5
28	79245.0
29	91819.5
30	103750.0
31	114509.0
32	125897.0
33	136473.0
34	147167.0
35	158007.5
36	168806.5
37	178813.0
38	186016.0
39	191928.0
40	198919.0
41	204127.0
42	205971.5
43	208401.0
44	210824.5
45	213264.0
46	213546.0
47	210588.5
48	206377.0
49	199792.0
50	191893.5
51	182458.5
52	171172.0
53	159465.0
54	147796.5
55	134598.5
56	121161.5
57	107685.5
58	93578.5
59	80331.0
60	66674.0
61	53219.5
62	41653.0
63	31864.0
64	23786.0
65	17710.5
66	13355.0
67	10326.5
68	7830.5
69	5479.5
70	3840.5
71	2819.5
72	1995.5
73	1346.5
74	920.5
75	613.0
76	396.5
77	251.5
78	174.5
79	120.5
80	82.0
81	57.5
82	37.5
83	25.0
84	18.0
85	12.0
86	10.0
87	8.5
88	5.5
89	3.5
90	2.5
91	2.0
92	1.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	0.0
3	0.0034311188675602328
4	0.012161184625257867
5	0.017358619122863902
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0016246947097097036
26-27	0.03441678133001356
28-29	0.08065500730459485
30-31	0.07869223263171099
32-33	0.034371922783939954
34-35	8.809805518421201E-4
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	1.2832396891824536
56-57	1.1338696385541076E-5
58-59	0.0
60-61	0.0
62-63	0.0
64-65	5.314981462904619
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.008512720011062819
74-75	0.03010381117005484
76-77	0.004915635407321839
78-79	0.0
80-81	0.0
82-83	0.004099883678941263
84-85	0.03825945415448202
86-87	0.04191042071521465
88-89	0.013837301957875981
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	138.0
20-21	353.0
22-23	651.0
24-25	1543.0
26-27	3178.0
28-29	5889.0
30-31	12249.0
32-33	15836.0
34-35	21581.0
36-37	33123.0
38-39	38990.0
40-41	39359.0
42-43	44701.0
44-45	52531.0
46-47	53175.0
48-49	48349.0
50-51	43962.0
52-53	44198.0
54-55	44350.0
56-57	46863.0
58-59	46999.0
60-61	46612.0
62-63	46353.0
64-65	44228.0
66-67	47134.0
68-69	48601.0
70-71	48253.0
72-73	44380.0
74-75	44254.0
76-77	45126.0
78-79	47476.0
80-81	48602.0
82-83	45622.0
84-85	44288.0
86-87	46694.0
88-89	50043.0
90-91	52023.0
92-93	53051.0
94-95	60420.0
96-97	150565.0
98-99	278381.0
100-101	3035383.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.92851046042672
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.23595888646877	80.2224196531593
2	6.410851285593981	11.27393208651181
3	1.4425330428869714	3.8051934525299473
4	0.4537296969167337	1.5958310560619706
5	0.19380166134374366	0.8520345703355722
6	0.10080375097695174	0.531811420333629
7	0.050759417618409367	0.31242399881178334
8	0.031955000481456886	0.22478044752773782
9	0.021079026358220714	0.16681026506709787
>10	0.05642684995393577	0.7337251093910419
>50	0.001378322857256584	0.08254704521772803
>100	6.249240059673991E-4	0.14579754597760183
>500	9.813453755908732E-5	0.0526933490747739
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.030247850627357E-5	0.0
2	0.0	0.0	0.0	4.060495701254714E-5	0.0
3	0.0	0.0	0.0	4.060495701254714E-5	0.0
4	0.0	0.0	0.0	4.060495701254714E-5	0.0
5	0.0	0.0	0.0	4.060495701254714E-5	0.0
6	0.0	0.0	0.0	4.060495701254714E-5	0.0
7	0.0	2.030247850627357E-5	0.0	4.060495701254714E-5	2.030247850627357E-5
8	0.0	2.030247850627357E-5	0.0	4.060495701254714E-5	2.030247850627357E-5
9	0.0	4.060495701254714E-5	0.0	6.09074355188207E-5	2.030247850627357E-5
10-11	2.030247850627357E-5	4.060495701254714E-5	0.0	6.09074355188207E-5	2.030247850627357E-5
12-13	2.030247850627357E-5	4.060495701254714E-5	0.0	6.09074355188207E-5	3.0453717759410353E-5
14-15	2.030247850627357E-5	8.120991402509427E-5	0.0	1.0151239253136784E-4	4.060495701254714E-5
16-17	4.060495701254714E-5	8.120991402509427E-5	0.0	1.4211734954391498E-4	4.060495701254714E-5
18-19	4.060495701254714E-5	8.120991402509427E-5	0.0	2.3347850282214602E-4	4.060495701254714E-5
20-21	4.060495701254714E-5	1.0151239253136784E-4	0.0	2.7408345983469316E-4	4.060495701254714E-5
22-23	6.0907435518820706E-5	1.218148710376414E-4	0.0	2.8423469908782996E-4	7.105867477195749E-5
24-25	8.120991402509427E-5	1.3196611029077818E-4	0.0	3.248396561003771E-4	8.120991402509427E-5
26-27	1.0151239253136784E-4	1.4211734954391498E-4	0.0	4.0604957012547137E-4	8.120991402509427E-5
28-29	1.218148710376414E-4	1.4211734954391498E-4	0.0	8.933090542760369E-4	8.120991402509427E-5
30-31	1.218148710376414E-4	1.4211734954391498E-4	0.0	0.0026596246843218372	8.120991402509427E-5
32-33	1.3196611029077818E-4	1.5226858879705175E-4	0.0	0.005491820435947	8.120991402509427E-5
34-35	1.4211734954391498E-4	1.8272230655646212E-4	0.0	0.00972488720450504	1.0151239253136784E-4
36-37	1.6241982805018855E-4	2.0302478506273569E-4	0.0	0.016384100154562767	1.0151239253136784E-4
38-39	2.1317602431587248E-4	2.436297420752828E-4	0.0	0.02634246586188995	1.0151239253136784E-4
40-41	2.2332726356900925E-4	2.8423469908782996E-4	0.0	0.03599629439162304	1.0151239253136784E-4
42-43	2.6393222058155636E-4	2.9438593834096676E-4	0.0	0.04477711634558636	1.0151239253136784E-4
44-45	3.248396561003771E-4	3.654446131129242E-4	0.0	0.05347672838552457	1.0151239253136784E-4
46-47	3.248396561003771E-4	4.263520486317449E-4	0.0	0.06268390238811963	1.218148710376414E-4
48-49	3.3499089535351384E-4	4.263520486317449E-4	0.0	0.07195198382623352	1.218148710376414E-4
50-51	3.4514213460665064E-4	4.568057663911553E-4	0.0	0.08099673800077839	1.218148710376414E-4
52-53	3.5529337385978744E-4	4.6695700564429205E-4	0.0	0.09030542439590483	1.218148710376414E-4
54-55	3.7559585236606103E-4	4.872594841505656E-4	0.0	0.09817263481708582	1.218148710376414E-4
56-57	3.857470916191978E-4	5.075619626568391E-4	0.0	0.10361369905676715	1.218148710376414E-4
58-59	3.857470916191978E-4	5.075619626568391E-4	0.0	0.10956232525910531	1.218148710376414E-4
60-61	3.857470916191978E-4	5.075619626568391E-4	0.0	0.115328229154887	1.218148710376414E-4
62-63	3.857470916191978E-4	5.075619626568391E-4	0.0	0.12101292313664361	1.218148710376414E-4
64-65	3.857470916191978E-4	5.481669196693863E-4	0.0	0.12661640720437511	1.218148710376414E-4
66-67	3.857470916191978E-4	5.481669196693863E-4	0.0	0.13319441024040773	1.3196611029077818E-4
68-69	3.857470916191978E-4	5.786206374287967E-4	0.0	0.1398333207119592	1.4211734954391498E-4
70-71	3.857470916191978E-4	5.989231159350703E-4	0.0	0.14557892212923462	1.4211734954391498E-4
72-73	3.857470916191978E-4	6.192255944413437E-4	0.0	0.15074590290908124	1.4211734954391498E-4
74-75	3.857470916191978E-4	6.395280729476174E-4	0.0	0.1544815589542356	1.4211734954391498E-4
76-77	3.857470916191978E-4	6.902842692133013E-4	0.0	0.1578314679077707	1.4211734954391498E-4
78-79	3.857470916191978E-4	7.105867477195749E-4	0.0	0.1599429256724232	1.4211734954391498E-4
80-81	3.857470916191978E-4	7.105867477195749E-4	0.0	0.16058245374537078	1.5226858879705175E-4
82-83	3.857470916191978E-4	7.105867477195749E-4	0.0	0.16125243553607782	1.8272230655646212E-4
84-85	3.857470916191978E-4	7.105867477195749E-4	0.0	0.16140470412487484	1.8272230655646212E-4
86-87	3.857470916191978E-4	7.308892262258485E-4	0.0	0.16153667023516566	1.928735458095989E-4
88	3.857470916191978E-4	7.308892262258485E-4	0.0	0.1615468214744188	2.0302478506273569E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6065	0.0	39.573994	1
GTATCAA	9790	0.0	30.719437	1
TCAACGC	10860	0.0	26.969456	4
ATCAACG	10890	0.0	26.668095	3
CAACGCA	10980	0.0	26.63411	5
AACGCAG	11330	0.0	25.917028	6
TATCAAC	11885	0.0	24.852575	2
TACATGG	11810	0.0	23.156487	2
GTACATG	12230	0.0	23.070597	1
ACGGGCG	290	0.0	22.78921	5
ACGCAGA	13135	0.0	22.292648	7
CGCAGAG	13295	0.0	22.024366	8
ACATGGG	12010	0.0	21.842144	3
CATGGGG	8720	0.0	20.323408	4
CGTACGA	220	0.0	19.814709	60-61
GGCGTCG	335	1.2732926E-11	18.492344	8
GCAGAGT	16275	0.0	17.94089	9
GAGTACT	10425	0.0	17.411186	12-13
AGAGTAC	14170	0.0	17.225157	10-11
CAGAGTA	15550	0.0	16.732302	10-11
>>END_MODULE