FastQCFastQC Report
Fri 27 May 2016
SRR2139894_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139894_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5860264
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72300.049.603211
GTATCAA134750.035.9688841
ATCAACG161850.029.2679773
TCGACGG2203.6958372E-629.08169294
TCAACGC163600.029.0535764
CAACGCA165150.028.7272425
TATCAAC169800.028.1610682
AACGCAG171250.027.7229716
ACGCAGA200650.023.6001247
CGCAGAG203100.023.3554618
GTACATG187050.021.5424651
TACATGG184200.021.3459912
TGCGCGA3455.0000635E-621.19410994
ACATGGG184700.020.9139563
GCAGAGT238750.019.8339889
CATGGGG127550.019.3179284
GAGTACT147550.017.96122412-13
AGAGTAC218500.017.76558310-11
CAGAGTA233800.017.66349810-11
AGTACTT157850.016.35146512-13
Produced by FastQC (version 0.11.5)