##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139894_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5860264
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.71155343854816	33.0	33.0	33.0	33.0	33.0
2	32.725071771510635	33.0	33.0	33.0	33.0	33.0
3	32.63358749708204	33.0	33.0	33.0	33.0	33.0
4	36.611982668357605	37.0	37.0	37.0	37.0	37.0
5	36.67107266839856	37.0	37.0	37.0	37.0	37.0
6	36.683239355769636	37.0	37.0	37.0	37.0	37.0
7	36.66834770583714	37.0	37.0	37.0	37.0	37.0
8	36.6708040798162	37.0	37.0	37.0	37.0	37.0
9	36.66966505263244	37.0	37.0	37.0	37.0	37.0
10-11	36.638838199098196	37.0	37.0	37.0	37.0	37.0
12-13	36.61189478835766	37.0	37.0	37.0	37.0	37.0
14-15	38.762115239176936	40.0	37.0	40.0	37.0	40.0
16-17	38.72308517158954	40.0	37.0	40.0	37.0	40.0
18-19	38.740996480704624	40.0	37.0	40.0	37.0	40.0
20-21	38.76717064341281	40.0	37.0	40.0	37.0	40.0
22-23	38.80274643453755	40.0	37.0	40.0	37.0	40.0
24-25	38.82198461646654	40.0	37.0	40.0	37.0	40.0
26-27	38.54525235857288	40.0	37.0	40.0	37.0	40.0
28-29	38.51392770870713	40.0	37.0	40.0	37.0	40.0
30-31	38.52574532596654	40.0	37.0	40.0	37.0	40.0
32-33	38.523677945620086	40.0	37.0	40.0	37.0	40.0
34-35	38.35804259446329	40.0	37.0	40.0	37.0	40.0
36-37	38.427466582333864	40.0	37.0	40.0	37.0	40.0
38-39	38.36794590761561	40.0	37.0	40.0	37.0	40.0
40-41	38.37742432493469	40.0	37.0	40.0	37.0	40.0
42-43	38.33596195027327	40.0	37.0	40.0	37.0	40.0
44-45	38.26796985588318	40.0	37.0	40.0	37.0	40.0
46-47	38.200667685651325	40.0	37.0	40.0	37.0	40.0
48-49	37.979721797327535	40.0	37.0	40.0	33.0	40.0
50-51	38.084527283334786	40.0	37.0	40.0	35.0	40.0
52-53	38.05334477481201	40.0	37.0	40.0	35.0	40.0
54-55	33.59053469592148	40.0	33.0	40.0	10.5	40.0
56-57	36.77185594004479	37.0	35.0	40.0	30.0	40.0
58-59	37.54734799451296	38.5	37.0	40.0	33.0	40.0
60-61	37.59356866255995	40.0	37.0	40.0	33.0	40.0
62-63	37.374242937637526	38.5	37.0	40.0	33.0	40.0
64-65	32.56131719690273	37.0	33.0	40.0	8.5	40.0
66-67	35.59748893527069	37.0	33.0	40.0	30.0	40.0
68-69	36.44860221408881	37.0	37.0	40.0	33.0	40.0
70-71	36.38415274178695	37.0	37.0	40.0	33.0	40.0
72-73	35.746519363099225	37.0	37.0	37.0	33.0	40.0
74-75	35.80399029150153	37.0	37.0	37.0	33.0	40.0
76-77	33.98403487470442	35.0	33.0	37.0	30.0	37.0
78-79	35.48617599465906	37.0	33.0	37.0	33.0	37.0
80-81	35.66967377619657	37.0	37.0	37.0	33.0	37.0
82-83	35.590399450901245	37.0	37.0	37.0	33.0	37.0
84-85	35.462210471919335	37.0	33.0	37.0	33.0	37.0
86-87	35.35159397903047	37.0	33.0	37.0	33.0	37.0
88-89	35.26519068071329	37.0	33.0	37.0	33.0	37.0
90-91	35.16979704516848	37.0	33.0	37.0	33.0	37.0
92-93	35.10458576866061	37.0	33.0	37.0	33.0	37.0
94-95	35.05220402015127	37.0	33.0	37.0	33.0	37.0
96-97	35.02076717870236	37.0	33.0	37.0	33.0	37.0
98-99	34.987505743126654	37.0	33.0	37.0	33.0	37.0
100	34.87576062264067	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	6.0
20	37.0
21	117.0
22	460.0
23	1499.0
24	3311.0
25	7009.0
26	13046.0
27	21601.0
28	33438.0
29	48011.0
30	67461.0
31	93009.0
32	127342.0
33	175404.0
34	257274.0
35	423344.0
36	900493.0
37	1438284.0
38	2135358.0
39	113760.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.58040252111509	20.422885385368303	12.995540815226073	24.001171278290535
2	14.54304788999267	22.075080576574706	41.20773398604568	22.174137547386945
3	17.90472449262203	27.43249707043199	30.764397544811256	23.89838089213473
4	11.642112042946854	18.27389986314581	40.45880200787744	29.6251860860299
5	12.302814835345727	38.758792253720046	36.352608009486495	12.585784901447738
6	28.419777675544992	39.30664215810073	19.602376275198523	12.671203891155757
7	25.358721040553807	33.2593036764214	24.46806150712664	16.913913775898152
8	24.044889445253663	36.857622796515656	22.21812873959262	16.87935901863807
9	25.7187048228544	16.103540727857993	21.766920398125407	36.4108340511622
10-11	23.481510389293042	27.146703629734088	30.00270124349347	19.369084737479405
12-13	24.469529017805343	24.950462982555052	29.94223468430774	20.63777331533187
14-15	21.514218472068837	26.23572931185353	27.6456231323367	24.604429083740936
16-17	20.903375684098872	29.299806288590414	28.275944564954752	21.520873462355965
18-19	20.881883478286987	28.51322909684615	30.111433546338528	20.49345387852834
20-21	21.806886462438342	27.793832229411436	29.84665853766959	20.55262277048063
22-23	21.439355211570778	27.75235962983167	29.717994657879803	21.090290500717746
24-25	21.1429822379053	27.843571690405362	29.89605101390695	21.11739505778239
26-27	21.226950340677185	28.01235869248908	29.621272680885273	21.139418285948466
28-29	21.056208747859216	28.228786216894054	29.67399396625636	21.041011068990365
30-31	21.372294159774274	28.05787286745833	29.577099038213824	20.992733934553577
32-33	21.116981768683456	28.20191324588076	29.53839715558237	21.142707829853418
34-35	21.25394916328645	28.300048734401308	29.487097366816418	20.958904735495825
36-37	21.272910506017396	28.193703124300995	29.457688889104674	21.07569748057694
38-39	21.11463874891833	28.34355040937509	29.4730744125469	21.068736429159678
40-41	21.29036595608167	28.264637215377125	29.340339217715066	21.10465761082614
42-43	21.28744097270356	28.303657687829837	29.139403310695883	21.269498028770716
44-45	21.409668987412033	28.503447236803975	28.854866068146002	21.232017707637986
46-47	21.39397085078867	28.50277390895882	28.606435432264625	21.49681980798789
48-49	21.430862822396104	28.602025845786223	28.51178892068481	21.455322411132858
50-51	21.35865554043514	28.68165309571823	28.45407023658213	21.505621127264508
52-53	21.513241251126196	28.607316917445253	28.302769323902826	21.57667250752572
54-55	22.567193697514266	28.436561226605317	27.638718528653072	21.357526547227344
56-57	21.56964482508423	28.65946549019528	28.25200886861166	21.51888081610883
58-59	21.470953131915504	28.742339927902783	28.289204145467483	21.49750279471423
60-61	21.579902607738653	28.658027725435176	28.26011932395514	21.50195034287103
62-63	21.535112534257845	28.685993507561804	28.21511116996304	21.56378278821731
64-65	21.97534895023224	28.615781681705148	27.869805889575545	21.539063478487066
66-67	21.5949844821936	28.6738192136761	28.078653970438427	21.652542333691876
68-69	21.600459321362266	28.682614330455948	28.15181545290379	21.565110895277996
70-71	21.554698531045176	28.432590962397363	28.21818950489782	21.794521001659646
72-73	21.67516499409451	28.528729669182447	28.13570006114435	21.660405275578693
74-75	21.482329892507188	28.632281346947735	28.151533134995617	21.733855625549463
76-77	21.57243425276124	28.605794827861974	28.08689393458032	21.734876984796465
78-79	21.588847018844852	28.660029731368926	28.04804518191458	21.703078067871644
80-81	21.510410114232073	28.569811377655867	28.122713563699957	21.7970649444121
82-83	21.655515035986035	28.502744086280664	28.219685298517238	21.622055579216063
84-85	21.797856112423208	28.46789437720849	28.087270871393464	21.646978638974833
86-87	21.523245620668547	28.580789551156847	28.135678231119527	21.760286597055078
88-89	21.639016586243688	28.54985634196201	28.0877561216063	21.723370950188006
90-91	21.700563417581424	28.537570647485417	28.136127925738048	21.62573800919511
92-93	21.732265462222795	28.49467779576861	28.116653679498103	21.656403062510496
94-95	21.540577678896195	28.586742893523727	28.186918625102475	21.685760802477606
96-97	21.638309541200982	28.722252690913685	28.25986073563388	21.379577032251454
98-99	22.63518658295292	30.621609677511184	28.46287231534549	18.280331424190408
100	28.55765086289542	41.83715964677352	29.605189490331064	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1383.0
1	1308.5
2	2052.0
3	3095.0
4	4262.0
5	7215.0
6	9725.0
7	11107.0
8	11695.0
9	11106.5
10	9934.0
11	8704.5
12	9240.0
13	12005.0
14	18467.5
15	27509.0
16	34826.5
17	38856.0
18	39803.0
19	38481.5
20	36197.0
21	34381.5
22	34640.0
23	38245.0
24	45718.5
25	57077.5
26	71867.0
27	87859.5
28	103024.5
29	118204.5
30	131959.0
31	144513.0
32	158340.5
33	171949.5
34	184525.0
35	196561.5
36	208965.0
37	219837.5
38	226563.5
39	230210.0
40	234047.0
41	236600.0
42	237890.0
43	240621.5
44	242748.0
45	243789.5
46	242117.0
47	240016.0
48	237129.0
49	230496.0
50	223131.5
51	213740.5
52	201910.5
53	190557.5
54	178236.5
55	163913.0
56	148052.5
57	131422.5
58	115056.5
59	98039.0
60	82075.0
61	67048.5
62	52637.5
63	40753.5
64	30688.5
65	23089.0
66	17644.5
67	13448.5
68	10158.0
69	7256.5
70	5130.5
71	3623.5
72	2504.5
73	1765.0
74	1242.5
75	839.5
76	575.5
77	360.5
78	237.5
79	171.0
80	118.0
81	86.0
82	63.5
83	43.5
84	29.0
85	22.0
86	18.0
87	13.5
88	12.5
89	10.5
90	6.0
91	3.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	0.0
3	0.0031568543669704984
4	0.012678609700859893
5	0.01684224464972909
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0021507107117427183
26-27	0.035630475848292216
28-29	0.08330823860286214
30-31	0.08109471791142879
32-33	0.035454172326683284
34-35	0.0011034732942652062
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	1.2362665738353367
56-57	1.64441069640793E-4
58-59	0.0
60-61	0.0
62-63	0.0
64-65	5.147569424172144
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.00928675181267991
74-75	0.031091520063766826
76-77	0.004852888997120364
78-79	0.0
80-81	0.0
82-83	0.004655805858373125
84-85	0.0411409335985563
86-87	0.044836885029484415
88-89	0.014895035174512634
90-91	4.82822211704745E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	113.0
20-21	353.0
22-23	755.0
24-25	2059.0
26-27	4214.0
28-29	8163.0
30-31	16687.0
32-33	21644.0
34-35	29114.0
36-37	44112.0
38-39	52909.0
40-41	53721.0
42-43	60296.0
44-45	71437.0
46-47	71228.0
48-49	64637.0
50-51	58940.0
52-53	57634.0
54-55	58043.0
56-57	61446.0
58-59	62093.0
60-61	61650.0
62-63	60602.0
64-65	57753.0
66-67	61252.0
68-69	63428.0
70-71	62970.0
72-73	57064.0
74-75	57453.0
76-77	57886.0
78-79	61645.0
80-81	62533.0
82-83	58146.0
84-85	57159.0
86-87	59558.0
88-89	62819.0
90-91	65816.0
92-93	66931.0
94-95	74143.0
96-97	177024.0
98-99	1167102.0
100-101	2607732.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.55495249429353
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.10283950772411	77.98846993192015
2	7.091512732548355	12.276110953568011
3	1.6921343310265493	4.3938781990789835
4	0.5822965860881827	2.016026133858078
5	0.23277587199616087	1.0073952271222728
6	0.1134249770404632	0.5890496099642176
7	0.0668404432479023	0.40497599730137934
8	0.034049799209911295	0.2357743002443284
9	0.02304500425456709	0.17951933236363618
>10	0.05951755411928838	0.738056849245492
>50	0.0010364893790328004	0.05762269521394968
>100	4.984368554306037E-4	0.09335219012184509
>500	2.8266510059135404E-5	0.019768579997611314
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.7064077659299992E-5	0.0	0.0	0.0
3	0.0	1.7064077659299992E-5	0.0	0.0	0.0
4	0.0	1.7064077659299992E-5	0.0	3.4128155318599984E-5	0.0
5	0.0	1.7064077659299992E-5	0.0	3.4128155318599984E-5	0.0
6	0.0	1.7064077659299992E-5	0.0	3.4128155318599984E-5	1.7064077659299992E-5
7	0.0	1.7064077659299992E-5	0.0	5.119223297789997E-5	1.7064077659299992E-5
8	0.0	3.4128155318599984E-5	0.0	5.119223297789997E-5	1.7064077659299992E-5
9	0.0	8.532038829649995E-5	0.0	5.119223297789997E-5	1.7064077659299992E-5
10-11	0.0	8.532038829649995E-5	0.0	5.119223297789997E-5	1.7064077659299992E-5
12-13	0.0	1.0238446595579995E-4	0.0	6.825631063719997E-5	2.5596116488949987E-5
14-15	0.0	1.3651262127439994E-4	0.0	6.825631063719997E-5	5.972427180754997E-5
16-17	0.0	1.3651262127439994E-4	0.0	7.678834946684996E-5	1.1091650478544994E-4
18-19	0.0	1.3651262127439994E-4	0.0	8.532038829649995E-5	1.1944854361509993E-4
20-21	0.0	1.3651262127439994E-4	0.0	1.0238446595579995E-4	1.1944854361509993E-4
22-23	0.0	1.706407765929999E-4	0.0	1.0238446595579995E-4	1.4504466010404991E-4
24-25	0.0	1.8770485425229988E-4	0.0	1.365126212743999E-4	1.706407765929999E-4
26-27	0.0	2.047689319115999E-4	0.0	1.5357669893369992E-4	1.706407765929999E-4
28-29	0.0	2.3889708723019988E-4	0.0	5.375184462679497E-4	1.706407765929999E-4
30-31	0.0	3.0715339786739984E-4	0.0	0.0018002601930561491	1.706407765929999E-4
32-33	0.0	3.0715339786739984E-4	0.0	0.0038906097063203977	1.706407765929999E-4
34-35	0.0	3.924737861638998E-4	0.0	0.006706182520104896	1.706407765929999E-4
36-37	0.0	4.777941744603997E-4	0.0	0.011100182517374643	1.706407765929999E-4
38-39	0.0	5.460504850975996E-4	0.0	0.017678384455034793	1.962368930819499E-4
40-41	0.0	6.484349510533997E-4	0.0	0.024350438819821088	2.047689319115999E-4
42-43	0.0	6.569669898830496E-4	0.0	0.030211949495790633	2.047689319115999E-4
44-45	0.0	7.593514558388495E-4	0.0	0.03610758832707878	2.047689319115999E-4
46-47	0.0	8.873320382835995E-4	0.0	0.04249808541048662	2.133009707412499E-4
48-49	0.0	9.043961159428995E-4	0.0	0.04904215919282817	2.3889708723019986E-4
50-51	1.7064077659299992E-5	9.641203877504494E-4	0.0	0.05575687375176272	2.3889708723019986E-4
52-53	1.7064077659299992E-5	9.726524265800994E-4	0.0	0.06201939025272582	2.730252425487998E-4
54-55	1.7064077659299992E-5	9.811844654097493E-4	0.0	0.06769319607444306	3.0715339786739984E-4
56-57	1.7064077659299992E-5	9.897165042393994E-4	0.0	0.07387039218710965	3.0715339786739984E-4
58-59	1.7064077659299992E-5	0.0010238446595579995	0.0	0.08029501742583611	3.0715339786739984E-4
60-61	1.7064077659299992E-5	0.0010494407760469494	0.0	0.0866087261597771	3.0715339786739984E-4
62-63	1.7064077659299992E-5	0.0011091650478544993	0.0	0.0927347300394658	3.2421747552669985E-4
64-65	1.7064077659299992E-5	0.0011774213584916993	0.0	0.09845119605533129	3.2421747552669985E-4
66-67	1.7064077659299992E-5	0.0011774213584916993	0.0	0.10531948731319954	3.2421747552669985E-4
68-69	1.7064077659299992E-5	0.0011859533973213494	0.0	0.11214511837691954	3.2421747552669985E-4
70-71	1.7064077659299992E-5	0.0011944854361509993	0.0	0.11859533973213493	3.498135920156498E-4
72-73	1.7064077659299992E-5	0.0012030174749806492	0.0	0.12357805040865052	4.2660194148249974E-4
74-75	1.7064077659299992E-5	0.0012286135914695994	0.0	0.12784406982347551	4.2660194148249974E-4
76-77	1.7064077659299992E-5	0.0012542097079585495	0.0	0.1317602756462849	4.2660194148249974E-4
78-79	1.7064077659299992E-5	0.0012627417467881994	0.0	0.13410658632443861	4.2660194148249974E-4
80-81	1.7064077659299992E-5	0.0012627417467881994	0.0	0.13501951447921118	4.4366601914179975E-4
82-83	1.7064077659299992E-5	0.0012712737856178493	0.0	0.13567648146909422	4.6073009680109976E-4
84-85	1.7064077659299992E-5	0.0012798058244474994	0.0	0.13579593001270934	4.6073009680109976E-4
86-87	2.5596116488949987E-5	0.0012968699021067994	0.0	0.13587271836217618	4.6073009680109976E-4
88	3.4128155318599984E-5	0.0013139339797660992	0.0	0.13588125040100582	4.6073009680109976E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7230	0.0	49.60321	1
GTATCAA	13475	0.0	35.968884	1
ATCAACG	16185	0.0	29.267977	3
TCGACGG	220	3.6958372E-6	29.081692	94
TCAACGC	16360	0.0	29.053576	4
CAACGCA	16515	0.0	28.727242	5
TATCAAC	16980	0.0	28.161068	2
AACGCAG	17125	0.0	27.722971	6
ACGCAGA	20065	0.0	23.600124	7
CGCAGAG	20310	0.0	23.355461	8
GTACATG	18705	0.0	21.542465	1
TACATGG	18420	0.0	21.345991	2
TGCGCGA	345	5.0000635E-6	21.194109	94
ACATGGG	18470	0.0	20.913956	3
GCAGAGT	23875	0.0	19.833988	9
CATGGGG	12755	0.0	19.317928	4
GAGTACT	14755	0.0	17.961224	12-13
AGAGTAC	21850	0.0	17.765583	10-11
CAGAGTA	23380	0.0	17.663498	10-11
AGTACTT	15785	0.0	16.351465	12-13
>>END_MODULE