FastQCFastQC Report
Fri 27 May 2016
SRR2139893_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139893_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2910875
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31800.046.9686281
GTATCAA58900.035.192511
ATCAACG68350.029.0556453
TCAACGC68700.029.0275654
CAACGCA68800.028.9794035
AACGCAG71700.027.9741156
TATCAAC73350.027.5262782
TACATGG72600.024.3913022
CGCAGAG82550.024.197138
ACGCAGA82650.024.1178177
GTACATG73700.024.0833911
ACATGGG74200.022.973183
CATGGGG53950.020.7825184
GCAGAGT99950.019.8605949
AGAGTAC90300.018.45657310-11
GAGTACT64650.018.16701712-13
CAGAGTA98150.017.67565310-11
TATACCG2901.3562385E-817.1164635
ATGGGGG38500.016.3310345
GTATAAG13100.016.1074451
Produced by FastQC (version 0.11.5)