##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139893_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2910875
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.71223875982308	33.0	33.0	33.0	33.0	33.0
2	32.72652999527634	33.0	33.0	33.0	33.0	33.0
3	32.6357294627904	33.0	33.0	33.0	33.0	33.0
4	36.61317988577318	37.0	37.0	37.0	37.0	37.0
5	36.67153587838708	37.0	37.0	37.0	37.0	37.0
6	36.68034972302143	37.0	37.0	37.0	37.0	37.0
7	36.66485781766651	37.0	37.0	37.0	37.0	37.0
8	36.66616326705888	37.0	37.0	37.0	37.0	37.0
9	36.668388371194226	37.0	37.0	37.0	37.0	37.0
10-11	36.63666492034182	37.0	37.0	37.0	37.0	37.0
12-13	36.60963644952119	37.0	37.0	37.0	37.0	37.0
14-15	38.76979533645382	40.0	37.0	40.0	37.0	40.0
16-17	38.727669686949795	40.0	37.0	40.0	37.0	40.0
18-19	38.739726199166924	40.0	37.0	40.0	37.0	40.0
20-21	38.76240544744111	40.0	37.0	40.0	37.0	40.0
22-23	38.7968795659671	40.0	37.0	40.0	37.0	40.0
24-25	38.82102599998396	40.0	37.0	40.0	37.0	40.0
26-27	38.54715022931515	40.0	37.0	40.0	37.0	40.0
28-29	38.517812704896954	40.0	37.0	40.0	37.0	40.0
30-31	38.53485930245596	40.0	37.0	40.0	37.0	40.0
32-33	38.5336804046692	40.0	37.0	40.0	37.0	40.0
34-35	38.369122116716895	40.0	37.0	40.0	37.0	40.0
36-37	38.43443398957559	40.0	37.0	40.0	37.0	40.0
38-39	38.37188480990983	40.0	37.0	40.0	37.0	40.0
40-41	38.3794667669588	40.0	37.0	40.0	37.0	40.0
42-43	38.3412319579043	40.0	37.0	40.0	37.0	40.0
44-45	38.27244722940236	40.0	37.0	40.0	37.0	40.0
46-47	38.204265458837114	40.0	37.0	40.0	37.0	40.0
48-49	37.98293352601799	40.0	37.0	40.0	33.0	40.0
50-51	38.08378873394733	40.0	37.0	40.0	35.0	40.0
52-53	38.049211886309706	40.0	37.0	40.0	35.0	40.0
54-55	33.5258935999621	40.0	33.0	40.0	10.5	40.0
56-57	36.75296872898297	37.0	35.0	40.0	30.0	40.0
58-59	37.536649194464026	38.5	37.0	40.0	33.0	40.0
60-61	37.58014280891447	40.0	37.0	40.0	33.0	40.0
62-63	37.35701796117637	37.0	37.0	40.0	33.0	40.0
64-65	32.48167525671326	37.0	33.0	40.0	8.5	40.0
66-67	35.55853737301865	37.0	33.0	40.0	30.0	40.0
68-69	36.417009249476415	37.0	37.0	40.0	33.0	40.0
70-71	36.350251646652275	37.0	37.0	40.0	33.0	40.0
72-73	35.70790267908574	37.0	37.0	37.0	33.0	40.0
74-75	35.76515422394155	37.0	37.0	37.0	33.0	40.0
76-77	33.95660947972921	35.0	33.0	37.0	30.0	37.0
78-79	35.453931725224926	37.0	33.0	37.0	33.0	37.0
80-81	35.642692565301886	37.0	37.0	37.0	33.0	37.0
82-83	35.56362771110296	37.0	37.0	37.0	33.0	37.0
84-85	35.43971563432836	37.0	33.0	37.0	33.0	37.0
86-87	35.331455965969994	37.0	33.0	37.0	33.0	37.0
88-89	35.236246883243155	37.0	33.0	37.0	33.0	37.0
90-91	35.14389845400073	37.0	33.0	37.0	33.0	37.0
92-93	35.07616402749321	37.0	33.0	37.0	33.0	37.0
94-95	35.022905924399346	37.0	33.0	37.0	33.0	37.0
96-97	34.990256788366366	37.0	33.0	37.0	33.0	37.0
98-99	34.95808263506199	37.0	33.0	37.0	33.0	37.0
100	34.91375944213309	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	18.0
21	63.0
22	296.0
23	778.0
24	1801.0
25	3677.0
26	6917.0
27	11143.0
28	16726.0
29	24829.0
30	34493.0
31	47234.0
32	64296.0
33	88712.0
34	129366.0
35	212379.0
36	453214.0
37	715496.0
38	1047408.0
39	52025.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.47310516597243	20.21797569459355	12.806458539099069	24.502460600334953
2	14.545351483660413	21.867239232189633	41.13835187014214	22.449057414007815
3	17.927651355609502	26.97690046083646	30.77342470817241	24.32202347538163
4	11.715268264810765	18.108669263470446	40.316667130504335	29.85939534121445
5	12.45482613861495	38.515892197076575	36.49065324547437	12.538628418834108
6	28.88791171039636	39.20831365139348	19.339339545669258	12.5644350925409
7	25.527032249753077	33.02999957057586	24.450414394297248	16.99255378537381
8	23.95990896208185	37.11866706746253	22.093185038862885	16.828238931592736
9	25.648816936488167	15.928234637351313	21.654485335165543	36.76846309099497
10-11	23.572826040279985	26.949147593077683	30.153167003048914	19.324859363593422
12-13	24.39115386267016	24.764271911366855	29.962124790655732	20.882449435307255
14-15	21.481272813157556	26.07745093829175	27.487490874736974	24.953785373813716
16-17	21.056417743805554	29.105612573538885	28.217889809765108	21.620079872890454
18-19	21.01294284364667	28.444969296173834	29.80356422038047	20.73852363979903
20-21	21.863052524445703	27.786294945955348	29.604600129689988	20.746052399908958
22-23	21.55242520807744	27.72395488863884	29.424323372529482	21.299296530754233
24-25	21.314551554474388	27.789921646559694	29.580751878252343	21.314774920713575
26-27	21.38541284992057	27.98295095519596	29.27944246893058	21.352193725952887
28-29	21.22234811669557	28.14910335477212	29.39520042526396	21.233348103268355
30-31	21.531204999751726	27.953757675904416	29.317385889025594	21.197651435318264
32-33	21.291911667633194	28.135430613030604	29.302069516569055	21.270588202767144
34-35	21.457069082195275	28.197214857054103	29.243567573414392	21.102148487336226
36-37	21.416414299431807	28.070617067504344	29.254977778211362	21.257990854852487
38-39	21.289787899642494	28.203044584346536	29.28255001924931	21.22461749676166
40-41	21.493947743830986	28.12186879065265	29.139370304955026	21.24481316056134
42-43	21.374079026418723	28.22231941925329	29.012629985173096	21.390971569154896
44-45	21.54185219633439	28.36221011749417	28.737385829109318	21.358551857062125
46-47	21.505178448136526	28.360096438654107	28.56006578907807	21.574659324131293
48-49	21.521459671944577	28.472698780314314	28.457735572851288	21.548105974889822
50-51	21.44367008157156	28.604301689094147	28.40846738524711	21.543560844087185
52-53	21.62713906050331	28.443653846370747	28.277499171952325	21.65170792117362
54-55	22.64904424895445	28.276434443441346	27.625530799284302	21.4489905083199
56-57	21.578336810700538	28.552766610986613	28.254019134178705	21.614877444134144
58-59	21.58144329297652	28.59141898588367	28.221493902882074	21.605643818257736
60-61	21.62408056784686	28.50355726936314	28.292743789444287	21.579618373345717
62-63	21.652088345280845	28.530850831970916	28.216222764748117	21.60083805800012
64-65	22.09116899920531	28.46434492795532	27.849255490814606	21.595230582024765
66-67	21.621344590312084	28.611790916984837	28.07940941296109	21.687455079741987
68-69	21.6277380888611	28.569191268865413	28.167542885088693	21.635527757184793
70-71	21.621887731630874	28.356020665164106	28.179536059784677	21.842555543420342
72-73	21.6807425663954	28.444435051909217	28.109379606541584	21.765442775153794
74-75	21.556583065965533	28.518068350951577	28.1678191779141	21.757529405168793
76-77	21.686537256554086	28.5202808329181	28.018879020720306	21.774302889807508
78-79	21.663023811810636	28.539712742290952	28.009530262086535	21.78773318381188
80-81	21.63895768351339	28.47055041369117	28.083346600737702	21.807145302057737
82-83	21.695049264349496	28.443704189520634	28.213911796438175	21.647334749691698
84-85	21.74121438012041	28.436281956633447	28.10276387152945	21.719739791716687
86-87	21.595705955994543	28.484874358519612	28.145090436841357	21.774329248644484
88-89	21.68896819000691	28.493149740003975	28.06680914808909	21.751072921900022
90-91	21.732520812607746	28.44064176208595	28.162169117064334	21.664668308241968
92-93	21.733254220188844	28.434120215875776	28.126307335647287	21.706318228288087
94-95	21.546766002466583	28.540693213054396	28.21734749877211	21.69519328570691
96-97	21.665416251121023	28.573322839810128	28.30530662305204	21.45595428601681
98-99	22.652762182351022	30.55891354957222	28.521553543413685	18.266770724663072
100	21.63629915079173	31.50570474947355	22.404622503685683	24.453373596049037
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	633.0
1	613.5
2	903.5
3	1340.0
4	1936.0
5	3314.5
6	4459.5
7	4999.5
8	5169.0
9	4829.5
10	4279.0
11	3787.0
12	4077.5
13	5384.5
14	8125.0
15	11825.5
16	14800.5
17	16450.5
18	16928.0
19	16378.5
20	15426.5
21	15033.5
22	15771.0
23	17986.0
24	21706.5
25	26960.5
26	33653.5
27	40893.5
28	47269.0
29	53643.5
30	59656.5
31	65310.0
32	71736.5
33	78218.5
34	84536.5
35	90153.5
36	96430.5
37	102500.0
38	107042.5
39	110851.0
40	113811.5
41	115731.5
42	117400.0
43	119247.5
44	120466.0
45	121965.5
46	121784.5
47	120837.0
48	120007.0
49	117181.0
50	113669.0
51	108625.5
52	102598.5
53	96669.5
54	90215.0
55	83081.0
56	75759.5
57	67676.0
58	59251.5
59	51212.5
60	42449.0
61	34103.5
62	26866.0
63	20735.0
64	15810.0
65	11913.0
66	8952.5
67	6886.0
68	5270.0
69	3716.0
70	2607.5
71	1877.0
72	1351.0
73	935.5
74	615.5
75	425.5
76	312.0
77	217.5
78	146.5
79	95.0
80	57.5
81	33.5
82	24.0
83	18.5
84	15.0
85	12.0
86	10.0
87	6.5
88	4.0
89	4.0
90	4.0
91	3.5
92	3.0
93	2.0
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	0.0
3	0.002782668441619788
4	0.012848370335380254
5	0.015562330914244
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0016150836441510262
26-27	0.03485760890209704
28-29	0.08084216204278166
30-31	0.07802571006583345
32-33	0.03404719587398426
34-35	6.413664363930036E-4
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	1.2436492627773057
56-57	1.9171367485974227E-5
58-59	0.0
60-61	0.0
62-63	0.0
64-65	5.215419183570007
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.00832704446202376
74-75	0.030688951089299133
76-77	0.005495742723968088
78-79	0.0
80-81	0.0
82-83	0.004234705562695925
84-85	0.038850091136212635
86-87	0.04260527270325348
88-89	0.014851424828685596
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	64.0
20-21	171.0
22-23	360.0
24-25	874.0
26-27	1759.0
28-29	3676.0
30-31	7408.0
32-33	9304.0
34-35	12769.0
36-37	19247.0
38-39	23207.0
40-41	22883.0
42-43	26193.0
44-45	31087.0
46-47	31317.0
48-49	28450.0
50-51	26029.0
52-53	25771.0
54-55	25378.0
56-57	27508.0
58-59	27650.0
60-61	27361.0
62-63	26956.0
64-65	25663.0
66-67	27274.0
68-69	28260.0
70-71	27960.0
72-73	25868.0
74-75	25866.0
76-77	26172.0
78-79	28127.0
80-81	28458.0
82-83	26216.0
84-85	26117.0
86-87	27354.0
88-89	29439.0
90-91	30546.0
92-93	31804.0
94-95	36076.0
96-97	89943.0
98-99	165458.0
100-101	1798852.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	90.03957868360814
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.64768019551751	83.41958090818063
2	5.562156243377121	10.016284094521533
3	1.1132400340815192	3.007069909272767
4	0.3463446622315037	1.2473890986656466
5	0.14193092117567158	0.6389700172416933
6	0.07022612302530507	0.3793878317869021
7	0.037248594152477076	0.2347693406832022
8	0.021888802001787902	0.15766868081039212
9	0.014475151626428795	0.11730028984424945
>10	0.04337478807017587	0.5711638189119715
>50	5.319989280314579E-4	0.031775233838641254
>100	9.02485812451885E-4	0.17864077624242133
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.435393137802207E-5
2	0.0	0.0	0.0	3.435393137802207E-5	3.435393137802207E-5
3	0.0	0.0	0.0	3.435393137802207E-5	3.435393137802207E-5
4	0.0	0.0	0.0	3.435393137802207E-5	3.435393137802207E-5
5	0.0	0.0	0.0	3.435393137802207E-5	3.435393137802207E-5
6	0.0	0.0	0.0	3.435393137802207E-5	3.435393137802207E-5
7	0.0	0.0	0.0	3.435393137802207E-5	3.435393137802207E-5
8	0.0	0.0	0.0	3.435393137802207E-5	3.435393137802207E-5
9	0.0	0.0	0.0	3.435393137802207E-5	3.435393137802207E-5
10-11	0.0	0.0	0.0	3.435393137802207E-5	3.435393137802207E-5
12-13	0.0	0.0	0.0	3.435393137802207E-5	3.435393137802207E-5
14-15	0.0	3.435393137802207E-5	0.0	3.435393137802207E-5	3.435393137802207E-5
16-17	0.0	3.435393137802207E-5	0.0	6.870786275604414E-5	3.435393137802207E-5
18-19	0.0	3.435393137802207E-5	0.0	6.870786275604414E-5	3.435393137802207E-5
20-21	0.0	3.435393137802207E-5	0.0	1.0306179413406621E-4	3.435393137802207E-5
22-23	0.0	3.435393137802207E-5	0.0	1.7176965689011037E-4	3.435393137802207E-5
24-25	0.0	3.435393137802207E-5	0.0	3.263623480912097E-4	3.435393137802207E-5
26-27	0.0	3.435393137802207E-5	0.0	5.153089706703311E-4	3.435393137802207E-5
28-29	0.0	3.435393137802207E-5	0.0	0.00132262635805385	3.435393137802207E-5
30-31	0.0	3.435393137802207E-5	0.0	0.005376390260660454	3.435393137802207E-5
32-33	0.0	3.435393137802207E-5	0.0	0.010168763687894534	3.435393137802207E-5
34-35	0.0	8.588482844505517E-5	0.0	0.0178983982479495	1.0306179413406621E-4
36-37	0.0	1.0306179413406621E-4	0.0	0.029887920298879204	1.0306179413406621E-4
38-39	0.0	1.0306179413406621E-4	0.0	0.04505518100227595	1.0306179413406621E-4
40-41	0.0	1.0306179413406621E-4	0.0	0.057577188989564994	1.0306179413406621E-4
42-43	0.0	1.2023875982307726E-4	0.0	0.06927470262378152	1.0306179413406621E-4
44-45	0.0	1.7176965689011037E-4	0.0	0.07940911238029802	1.0306179413406621E-4
46-47	0.0	1.7176965689011037E-4	0.0	0.0898011766221497	1.0306179413406621E-4
48-49	0.0	1.7176965689011037E-4	0.0	0.09972946279039807	1.0306179413406621E-4
50-51	0.0	2.7483145102417657E-4	0.0	0.10891913943401899	1.0306179413406621E-4
52-53	0.0	2.7483145102417657E-4	0.0	0.11810881607763989	1.2023875982307726E-4
54-55	0.0	2.7483145102417657E-4	0.0	0.12693777644179155	1.3741572551208828E-4
56-57	0.0	2.7483145102417657E-4	0.0	0.13452999527633444	1.3741572551208828E-4
58-59	0.0	2.7483145102417657E-4	0.0	0.1425344612874136	1.3741572551208828E-4
60-61	0.0	2.7483145102417657E-4	0.0	0.1498690256366213	1.3741572551208828E-4
62-63	0.0	2.9200841671318757E-4	0.0	0.15655086528964657	1.3741572551208828E-4
64-65	0.0	3.091853824021986E-4	0.0	0.16391978357023232	1.3741572551208828E-4
66-67	0.0	3.263623480912097E-4	0.0	0.17230214282646972	1.3741572551208828E-4
68-69	0.0	3.607162794692318E-4	0.0	0.18032378580323788	1.5459269120109934E-4
70-71	0.0	3.778932451582428E-4	0.0	0.18659337827972688	1.7176965689011037E-4
72-73	0.0	3.778932451582428E-4	0.0	0.19123115901575988	2.0612358826813243E-4
74-75	0.0	3.778932451582428E-4	0.0	0.1948383218104522	2.0612358826813243E-4
76-77	0.0	4.294241422252759E-4	0.0	0.19815347618843132	2.0612358826813243E-4
78-79	0.0	4.4660110791428696E-4	0.0	0.2006613131790269	2.0612358826813243E-4
80-81	0.0	4.4660110791428696E-4	0.0	0.20127968394383133	2.0612358826813243E-4
82-83	0.0	4.4660110791428696E-4	0.0	0.20193240864001374	2.404775196461545E-4
84-85	0.0	4.63778073603298E-4	0.0	0.20208700133121485	2.404775196461545E-4
86-87	0.0	4.80955039292309E-4	0.0	0.20212135526259287	2.404775196461545E-4
88	0.0	4.80955039292309E-4	0.0	0.20213853222828188	2.404775196461545E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3180	0.0	46.968628	1
GTATCAA	5890	0.0	35.19251	1
ATCAACG	6835	0.0	29.055645	3
TCAACGC	6870	0.0	29.027565	4
CAACGCA	6880	0.0	28.979403	5
AACGCAG	7170	0.0	27.974115	6
TATCAAC	7335	0.0	27.526278	2
TACATGG	7260	0.0	24.391302	2
CGCAGAG	8255	0.0	24.19713	8
ACGCAGA	8265	0.0	24.117817	7
GTACATG	7370	0.0	24.083391	1
ACATGGG	7420	0.0	22.97318	3
CATGGGG	5395	0.0	20.782518	4
GCAGAGT	9995	0.0	19.860594	9
AGAGTAC	9030	0.0	18.456573	10-11
GAGTACT	6465	0.0	18.167017	12-13
CAGAGTA	9815	0.0	17.675653	10-11
TATACCG	290	1.3562385E-8	17.116463	5
ATGGGGG	3850	0.0	16.331034	5
GTATAAG	1310	0.0	16.107445	1
>>END_MODULE