FastQCFastQC Report
Fri 27 May 2016
SRR2139892_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139892_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4480739
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52100.036.735991
GTATCAA88850.032.6156851
TCAACGC99000.028.9359384
ATCAACG98600.028.8431173
CAACGCA102450.028.0410065
AACGCAG106000.027.0202486
TATCAAC109800.026.3925652
ACGCAGA122000.023.4085627
CGCAGAG124250.022.9512568
GTACATG106400.022.631581
TACATGG104250.022.5806032
ACATGGG106300.021.6373983
CTTATAC71250.020.4527031
CATGGGG82600.019.8034024
GCAGAGT145800.019.359659
GAGTACT88500.018.26881212-13
AGAGTAC124600.018.00627110-11
TATACAC90950.017.711593
CAGAGTA140450.016.59488110-11
TTATACA92150.016.174382
Produced by FastQC (version 0.11.5)