##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139892_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4480739
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.717427861787975	33.0	33.0	33.0	33.0	33.0
2	32.73128271921217	33.0	33.0	33.0	33.0	33.0
3	32.643194794430116	33.0	33.0	33.0	33.0	33.0
4	36.619841950178305	37.0	37.0	37.0	37.0	37.0
5	36.6748163639971	37.0	37.0	37.0	37.0	37.0
6	36.681800256609456	37.0	37.0	37.0	37.0	37.0
7	36.66532551884856	37.0	37.0	37.0	37.0	37.0
8	36.66901665104796	37.0	37.0	37.0	37.0	37.0
9	36.67251585062196	37.0	37.0	37.0	37.0	37.0
10-11	36.639153162011894	37.0	37.0	37.0	37.0	37.0
12-13	36.613091501201026	37.0	37.0	37.0	37.0	37.0
14-15	38.7807614993866	40.0	37.0	40.0	37.0	40.0
16-17	38.7369279933511	40.0	37.0	40.0	37.0	40.0
18-19	38.74502766173169	40.0	37.0	40.0	37.0	40.0
20-21	38.76798039707593	40.0	37.0	40.0	37.0	40.0
22-23	38.80332667815871	40.0	37.0	40.0	37.0	40.0
24-25	38.82505874043098	40.0	37.0	40.0	37.0	40.0
26-27	38.54783266722248	40.0	37.0	40.0	37.0	40.0
28-29	38.52143770722135	40.0	37.0	40.0	37.0	40.0
30-31	38.54479664424643	40.0	37.0	40.0	37.0	40.0
32-33	38.54041981327467	40.0	37.0	40.0	37.0	40.0
34-35	38.36807876963881	40.0	37.0	40.0	37.0	40.0
36-37	38.433831373416496	40.0	37.0	40.0	37.0	40.0
38-39	38.35858592080106	40.0	37.0	40.0	37.0	40.0
40-41	38.374286954126354	40.0	37.0	40.0	37.0	40.0
42-43	38.33199452265086	40.0	37.0	40.0	37.0	40.0
44-45	38.26174352296043	40.0	37.0	40.0	37.0	40.0
46-47	38.18946199452632	40.0	37.0	40.0	37.0	40.0
48-49	37.96506461730398	40.0	37.0	40.0	33.0	40.0
50-51	38.04797802303364	40.0	37.0	40.0	33.0	40.0
52-53	38.01539509517413	40.0	37.0	40.0	33.0	40.0
54-55	33.425639191858465	38.5	33.0	40.0	10.5	40.0
56-57	36.649648819422815	37.0	35.0	40.0	30.0	40.0
58-59	37.45623612314477	38.5	37.0	40.0	33.0	40.0
60-61	37.49300545223121	40.0	37.0	40.0	33.0	40.0
62-63	37.26365212373422	37.0	37.0	40.0	33.0	40.0
64-65	32.253712522852766	37.0	33.0	40.0	8.5	40.0
66-67	35.41445713156065	37.0	33.0	40.0	30.0	40.0
68-69	36.32159069922472	37.0	37.0	40.0	33.0	40.0
70-71	36.25635668198541	37.0	37.0	40.0	33.0	40.0
72-73	35.60324409674901	37.0	37.0	37.0	33.0	40.0
74-75	35.671738050033525	37.0	35.0	37.0	33.0	40.0
76-77	33.85695218946621	35.0	33.0	37.0	30.0	37.0
78-79	35.36535203233541	37.0	33.0	37.0	33.0	37.0
80-81	35.56487570832322	37.0	35.0	37.0	33.0	37.0
82-83	35.49319082074916	37.0	35.0	37.0	33.0	37.0
84-85	35.37247143207433	37.0	33.0	37.0	33.0	37.0
86-87	35.26850744632716	37.0	33.0	37.0	33.0	37.0
88-89	35.18384327391093	37.0	33.0	37.0	33.0	37.0
90-91	35.09505404415397	37.0	33.0	37.0	33.0	37.0
92-93	35.037143370516446	37.0	33.0	37.0	33.0	37.0
94-95	34.98816207796689	37.0	33.0	37.0	33.0	37.0
96-97	34.963318811830405	37.0	33.0	37.0	33.0	37.0
98-99	34.93540926402864	37.0	33.0	37.0	33.0	37.0
100	34.89012223501106	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	18.0
21	128.0
22	417.0
23	1283.0
24	3071.0
25	6464.0
26	11532.0
27	19050.0
28	28173.0
29	40213.0
30	55053.0
31	74205.0
32	100564.0
33	138169.0
34	200171.0
35	331015.0
36	702547.0
37	1101760.0
38	1589624.0
39	77280.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.081986922246536	20.039863959940536	12.920167856239786	24.95798126157315
2	14.432574626640829	21.49779310957411	41.17653360305075	22.893098660734314
3	17.857451330617472	26.694570736124202	30.823291312816238	24.62468662044209
4	11.742442927577503	18.085970954931998	39.59491453505593	30.57667158243457
5	12.617973961688515	38.20079580108455	36.37417204499807	12.807058192228862
6	29.276085931360875	38.75563829984295	19.089351109270144	12.878924659526028
7	25.646260583354664	32.84980446305843	23.942970121669664	17.560964831917236
8	24.19495980462151	36.79953239856194	21.999830831476682	17.00567696533987
9	25.466200999433354	16.063354727869665	21.2242891183798	37.24615515431718
10-11	23.685579543910055	26.700372416246516	29.822100774001786	19.79194726584164
12-13	24.58197185776721	24.27348479793177	29.660553761332675	21.483989582968345
14-15	21.699233095255046	25.716717711074	26.881804541616905	25.702244652054045
16-17	21.57069403060522	28.920385677451865	27.5232612298998	21.985659062043116
18-19	21.466860712038795	28.07295850081873	29.062114530661127	21.39806625648135
20-21	22.104339760384395	27.40405120482936	28.869716575462224	21.621892459324023
22-23	21.748134653582028	27.585844772197728	28.821820217411148	21.844200356809093
24-25	21.65059325279381	27.64912188272861	29.01084797773679	21.689436886740783
26-27	21.70537630973642	27.751785436840176	28.821980841633575	21.720857411789833
28-29	21.409500413530154	27.58456344948111	28.950543672496643	22.055392464492098
30-31	21.66330502582011	27.502234965760763	28.90839042486131	21.92606958355782
32-33	21.482973744025795	27.781326192416934	28.95054876494224	21.785151298615027
34-35	21.620056201061654	27.984790985773735	28.939743148702124	21.455409664462486
36-37	21.71373666038725	27.759064867107693	28.664175471580606	21.86302300092445
38-39	21.65019191161532	27.694867470531882	28.87576489934167	21.779175718511127
40-41	21.79986042069916	27.570497628160005	28.83891192298669	21.790730028154144
42-43	21.77550315190572	27.62621299722239	28.80332149853848	21.794962352333407
44-45	21.726731328913118	27.86973010859472	28.565124177844964	21.8384143846472
46-47	21.986630494321428	27.62937529826904	28.214394023099835	22.169600184309697
48-49	21.84805089448423	27.736807977469258	28.262255456930564	22.152885671115946
50-51	21.640099817273082	27.93305441334601	28.361528679504648	22.065317089876256
52-53	21.78416082517975	27.960818879528	28.36600056074412	21.889019734548125
54-55	22.82039201299593	27.8590886330235	27.499106887285844	21.821412466694724
56-57	21.84702624430433	28.020270798755764	28.012203637737077	22.12049931920283
58-59	21.610264389431464	28.246591441045783	28.24173300436429	21.901411165158464
60-61	21.931584595789523	28.43614146693222	27.816943278278316	21.815330658999937
62-63	21.623923557115212	28.553507802330408	27.84608806640928	21.976480574145103
64-65	22.273386139161815	28.391411696372543	27.422944627128583	21.91225753733706
66-67	21.710400444527053	28.424507229474493	27.671601223488494	22.19349110250996
68-69	21.800752669705084	28.28010843198896	27.689371334851227	22.22976756345473
70-71	21.8223199162268	28.018755829595516	27.63847659446506	22.520447659712623
72-73	21.92166411595186	27.984639178171804	27.610013877503768	22.483682828372565
74-75	21.73074264114297	28.11466066570207	27.677965834130813	22.476630859024148
76-77	21.887160723758143	28.00946431373617	27.51566027877751	22.587714683728176
78-79	21.902153896140746	28.071474590434818	27.46950585124909	22.556865662175348
80-81	21.871624858975338	28.089071973582193	27.481565948574872	22.557737218867604
82-83	21.860183315430415	27.989824204508114	27.69006733730738	22.459925142754095
84-85	22.006481178046535	28.010366075302823	27.63847107460079	22.344681672049852
86-87	21.81795695687306	28.00319069436296	27.76786019350338	22.4109921552606
88-89	21.789232602958386	28.101220763850694	27.771346515196022	22.3382001179949
90-91	21.881401719055475	27.861480363715064	27.875059427137383	22.38205849009208
92-93	21.84715934124512	27.82924094327625	27.918834453974622	22.404765261504007
94-95	21.677686851683024	27.9191235917637	28.109195137752057	22.29399441880122
96-97	21.747455344663095	28.01414162532198	28.200742588268092	22.037660441746837
98-99	22.937761269710062	29.983190465404796	28.29308465395214	18.785963610933006
100	21.893047054562924	30.795872192879603	22.14841747075001	25.162663281807458
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	471.0
1	470.5
2	894.0
3	1440.0
4	2129.5
5	3791.0
6	5126.0
7	5822.5
8	6143.5
9	5802.0
10	5192.5
11	4641.5
12	4929.5
13	6501.0
14	10244.5
15	15246.5
16	19214.5
17	21479.0
18	22105.5
19	21515.0
20	20317.0
21	19583.0
22	20070.5
23	22362.0
24	26887.5
25	33472.0
26	42446.5
27	52622.5
28	62662.0
29	73567.0
30	84167.0
31	93656.5
32	103534.5
33	113293.5
34	122659.5
35	131099.0
36	140176.0
37	149684.5
38	156940.0
39	162353.0
40	167680.5
41	172624.0
42	175803.0
43	179895.5
44	184365.0
45	189400.0
46	196301.5
47	202518.5
48	201081.0
49	194228.0
50	187594.5
51	177713.0
52	166130.5
53	155631.0
54	145537.0
55	134378.5
56	122939.0
57	110274.5
58	96372.0
59	83175.5
60	69365.0
61	55769.0
62	44209.0
63	34502.0
64	25853.0
65	18846.0
66	14158.0
67	10970.5
68	8400.0
69	6126.5
70	4430.5
71	3162.5
72	2254.5
73	1565.5
74	1075.0
75	746.5
76	511.0
77	339.0
78	205.0
79	114.5
80	72.5
81	51.5
82	38.0
83	30.0
84	22.5
85	15.5
86	11.5
87	9.5
88	7.0
89	5.0
90	3.5
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	0.0
3	0.003347662070921783
4	0.013346012789408175
5	0.01618036667612195
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0017189128746685932
26-27	0.035808164708178526
28-29	0.08200132851314351
30-31	0.08053160482245227
32-33	0.03561484058629095
34-35	9.790245674399386E-4
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	1.262145387902027
56-57	4.911511145508061E-5
58-59	0.0
60-61	0.0
62-63	0.0
64-65	5.42117419816358
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.010242190800574586
74-75	0.03470416366978777
76-77	0.005487174451715753
78-79	0.0
80-81	0.0
82-83	0.004019976145290491
84-85	0.04145518966614725
86-87	0.04522922195959727
88-89	0.014750882281603801
90-91	2.9975848458896667E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	92.0
20-21	286.0
22-23	549.0
24-25	1152.0
26-27	2730.0
28-29	5065.0
30-31	10395.0
32-33	13411.0
34-35	17646.0
36-37	26798.0
38-39	29713.0
40-41	32449.0
42-43	35191.0
44-45	41128.0
46-47	41138.0
48-49	38359.0
50-51	33868.0
52-53	34533.0
54-55	34536.0
56-57	37879.0
58-59	41696.0
60-61	44312.0
62-63	49744.0
64-65	45405.0
66-67	49150.0
68-69	44754.0
70-71	43649.0
72-73	40102.0
74-75	39615.0
76-77	39856.0
78-79	43270.0
80-81	44381.0
82-83	41423.0
84-85	40315.0
86-87	42049.0
88-89	46055.0
90-91	48320.0
92-93	49507.0
94-95	56685.0
96-97	143103.0
98-99	261540.0
100-101	2788890.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.71901763850292
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.0911603236035	79.90427099137872
2	6.506553198196337	11.41496909516884
3	1.4620816432902688	3.847570963701312
4	0.4711808201627629	1.653260746991267
5	0.19414334349282755	0.8515031686122635
6	0.09910853595522144	0.5216222048149363
7	0.05557794884158757	0.3412670152322923
8	0.031405890270776116	0.2203915074092078
9	0.024649327398891065	0.19459933063925025
>10	0.06211738558005263	0.8150589438333096
>50	0.0012937466659716933	0.07390247148814146
>100	6.916019176228311E-4	0.14283061345021286
>500	3.6234624215889866E-5	0.01875294728023719
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.2317747139478554E-5	0.0	0.0	0.0
3	0.0	2.2317747139478554E-5	0.0	0.0	0.0
4	0.0	2.2317747139478554E-5	0.0	2.2317747139478554E-5	0.0
5	2.2317747139478554E-5	2.2317747139478554E-5	0.0	2.2317747139478554E-5	0.0
6	2.2317747139478554E-5	2.2317747139478554E-5	0.0	2.2317747139478554E-5	0.0
7	4.463549427895711E-5	4.463549427895711E-5	0.0	2.2317747139478554E-5	0.0
8	4.463549427895711E-5	4.463549427895711E-5	0.0	2.2317747139478554E-5	0.0
9	4.463549427895711E-5	1.1158873569739278E-4	0.0	4.463549427895711E-5	2.2317747139478554E-5
10-11	6.695324141843567E-5	1.2274760926713206E-4	0.0	1.004298621276535E-4	2.2317747139478554E-5
12-13	1.1158873569739278E-4	1.3390648283687133E-4	0.0	1.450653564066106E-4	3.347662070921783E-5
14-15	1.2274760926713206E-4	1.7854197711582843E-4	0.0	1.562242299763499E-4	6.695324141843567E-5
16-17	1.450653564066106E-4	1.7854197711582843E-4	0.0	2.454952185342641E-4	6.695324141843567E-5
18-19	1.562242299763499E-4	1.7854197711582843E-4	0.0	3.347662070921783E-4	6.695324141843567E-5
20-21	1.7854197711582843E-4	1.7854197711582843E-4	0.0	5.579436784869639E-4	6.695324141843567E-5
22-23	1.8970085068556773E-4	2.1201859782504626E-4	0.0	7.030090348935745E-4	1.004298621276535E-4
24-25	2.2317747139478556E-4	2.454952185342641E-4	0.0	7.364856556027923E-4	1.3390648283687133E-4
26-27	2.454952185342641E-4	2.6781296567374266E-4	0.0	9.2618650628836E-4	1.562242299763499E-4
28-29	2.454952185342641E-4	2.6781296567374266E-4	0.0	0.0019528028747043736	1.562242299763499E-4
30-31	2.6781296567374266E-4	3.347662070921783E-4	0.0	0.00509960522137085	1.562242299763499E-4
32-33	2.6781296567374266E-4	3.347662070921783E-4	0.0	0.011426686535413021	1.562242299763499E-4
34-35	2.901307128132212E-4	4.01719448510614E-4	0.0	0.018378664769360592	1.562242299763499E-4
36-37	3.124484599526998E-4	4.6867268992904966E-4	0.0	0.039725589908271824	1.562242299763499E-4
38-39	3.124484599526998E-4	4.6867268992904966E-4	0.0	0.0957989295962117	1.562242299763499E-4
40-41	3.124484599526998E-4	4.6867268992904966E-4	0.0	0.18156603185322778	1.562242299763499E-4
42-43	3.124484599526998E-4	4.6867268992904966E-4	0.0	0.23139040234211367	1.562242299763499E-4
44-45	3.124484599526998E-4	5.021493106382675E-4	0.0	0.2463209751784248	1.562242299763499E-4
46-47	3.124484599526998E-4	5.356259313474853E-4	0.0	0.2614970432332702	1.7854197711582843E-4
48-49	3.124484599526998E-4	5.356259313474853E-4	0.0	0.27713062510447495	1.7854197711582843E-4
50-51	3.124484599526998E-4	6.025791727659209E-4	0.0	0.2942371782868853	1.7854197711582843E-4
52-53	3.124484599526998E-4	6.025791727659209E-4	0.0	0.3467285195589388	1.7854197711582843E-4
54-55	3.124484599526998E-4	6.025791727659209E-4	0.0	0.38244807385567425	1.7854197711582843E-4
56-57	3.124484599526998E-4	6.025791727659209E-4	0.0	0.41810067491099123	1.7854197711582843E-4
58-59	3.124484599526998E-4	6.248969199053996E-4	0.0	0.4507626978496181	1.7854197711582843E-4
60-61	3.124484599526998E-4	6.248969199053996E-4	0.0	0.4641533461333053	1.7854197711582843E-4
62-63	3.124484599526998E-4	6.248969199053996E-4	0.0	0.47833627444044385	1.7854197711582843E-4
64-65	3.124484599526998E-4	6.472146670448781E-4	0.0	0.48552258901935597	1.8970085068556773E-4
66-67	3.124484599526998E-4	6.472146670448781E-4	0.0	0.4924299317590246	2.2317747139478556E-4
68-69	3.124484599526998E-4	6.695324141843567E-4	0.0	0.4981209572795916	2.2317747139478556E-4
70-71	3.124484599526998E-4	6.695324141843567E-4	0.0	0.5037338706851704	2.2317747139478556E-4
72-73	3.124484599526998E-4	6.695324141843567E-4	0.0	0.5089115880215295	2.454952185342641E-4
74-75	3.124484599526998E-4	6.806912877540959E-4	0.0	0.5130738478630422	2.454952185342641E-4
76-77	3.124484599526998E-4	7.25326782033053E-4	0.0	0.5160644259797323	2.454952185342641E-4
78-79	3.124484599526998E-4	7.364856556027923E-4	0.0	0.5184189483029473	2.454952185342641E-4
80-81	3.124484599526998E-4	7.364856556027923E-4	0.0	0.5192112283263988	2.566540921040034E-4
82-83	3.2360733352243906E-4	7.364856556027923E-4	0.0	0.5197691720048858	2.6781296567374266E-4
84-85	3.347662070921783E-4	7.364856556027923E-4	0.0	0.5199142373612924	2.6781296567374266E-4
86-87	3.347662070921783E-4	7.588034027422709E-4	0.0	0.5200035083498503	2.6781296567374266E-4
88	3.347662070921783E-4	8.03438897021228E-4	0.0	0.5200035083498503	2.6781296567374266E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5210	0.0	36.73599	1
GTATCAA	8885	0.0	32.615685	1
TCAACGC	9900	0.0	28.935938	4
ATCAACG	9860	0.0	28.843117	3
CAACGCA	10245	0.0	28.041006	5
AACGCAG	10600	0.0	27.020248	6
TATCAAC	10980	0.0	26.392565	2
ACGCAGA	12200	0.0	23.408562	7
CGCAGAG	12425	0.0	22.951256	8
GTACATG	10640	0.0	22.63158	1
TACATGG	10425	0.0	22.580603	2
ACATGGG	10630	0.0	21.637398	3
CTTATAC	7125	0.0	20.452703	1
CATGGGG	8260	0.0	19.803402	4
GCAGAGT	14580	0.0	19.35965	9
GAGTACT	8850	0.0	18.268812	12-13
AGAGTAC	12460	0.0	18.006271	10-11
TATACAC	9095	0.0	17.71159	3
CAGAGTA	14045	0.0	16.594881	10-11
TTATACA	9215	0.0	16.17438	2
>>END_MODULE