FastQCFastQC Report
Fri 27 May 2016
SRR2139891_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139891_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6363819
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73400.049.0662841
GTATCAA127700.035.4017941
TCAACGC148300.029.4889284
ATCAACG151500.028.8938963
CAACGCA152600.028.6865185
AACGCAG158050.027.643966
TATCAAC166450.026.6534422
ACGCAGA184450.023.7332447
GTACATG173800.023.4395221
CGCAGAG187400.023.3596428
TACATGG173650.022.925492
ACATGGG175100.022.2539543
CATGGGG127150.020.1986484
GCAGAGT229350.019.0890739
GAGTACT143300.018.8956312-13
GTGGTAT38250.018.742991
CAGAGTA219150.017.99518610-11
AGAGTAC202100.017.41591310-11
GAGTACA122750.016.8343351
ATGGGGG76550.016.7480185
Produced by FastQC (version 0.11.5)