##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139891_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6363819
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.566996955758796	33.0	33.0	33.0	33.0	33.0
2	32.56512292382923	33.0	33.0	33.0	33.0	33.0
3	32.56664606582934	33.0	33.0	33.0	33.0	33.0
4	36.466552709937226	37.0	37.0	37.0	37.0	37.0
5	36.4990767336406	37.0	37.0	37.0	37.0	37.0
6	36.49250033666891	37.0	37.0	37.0	37.0	37.0
7	36.47200352492741	37.0	37.0	37.0	37.0	37.0
8	36.45601611233758	37.0	37.0	37.0	37.0	37.0
9	36.49484515508691	37.0	37.0	37.0	37.0	37.0
10-11	36.44775951358767	37.0	37.0	37.0	37.0	37.0
12-13	36.37988666868118	37.0	37.0	37.0	37.0	37.0
14-15	38.41631235897815	40.0	37.0	40.0	37.0	40.0
16-17	38.377761843949365	40.0	37.0	40.0	37.0	40.0
18-19	38.38077780024856	40.0	37.0	40.0	37.0	40.0
20-21	38.37637880167459	40.0	37.0	40.0	37.0	40.0
22-23	38.37023673242611	40.0	37.0	40.0	37.0	40.0
24-25	38.37359218370631	40.0	37.0	40.0	37.0	40.0
26-27	38.22762346932959	40.0	37.0	40.0	37.0	40.0
28-29	38.15098970412847	40.0	37.0	40.0	35.0	40.0
30-31	38.11369127632837	40.0	37.0	40.0	35.0	40.0
32-33	38.040601330071155	40.0	37.0	40.0	33.0	40.0
34-35	38.00697654809394	40.0	37.0	40.0	33.0	40.0
36-37	37.972650960898065	40.0	37.0	40.0	33.0	40.0
38-39	37.93062017299684	40.0	37.0	40.0	33.0	40.0
40-41	37.902820609956535	40.0	37.0	40.0	33.0	40.0
42-43	37.81326500085728	40.0	37.0	40.0	33.0	40.0
44-45	37.77026341363245	40.0	37.0	40.0	33.0	40.0
46-47	37.74292496731944	40.0	37.0	40.0	33.0	40.0
48-49	37.72241930819364	40.0	37.0	40.0	33.0	40.0
50-51	37.66786401199497	40.0	37.0	40.0	33.0	40.0
52-53	37.572711060371304	40.0	37.0	40.0	33.0	40.0
54-55	37.37515720846906	40.0	37.0	40.0	33.0	40.0
56-57	37.20981312717205	40.0	37.0	40.0	33.0	40.0
58-59	37.15402336646379	37.0	37.0	40.0	33.0	40.0
60-61	37.02865421788056	37.0	37.0	40.0	33.0	40.0
62-63	36.85429824044855	37.0	37.0	40.0	33.0	40.0
64-65	36.7150620058993	37.0	37.0	40.0	33.0	40.0
66-67	36.50541404443801	37.0	37.0	40.0	33.0	40.0
68-69	36.29653119474959	37.0	37.0	40.0	33.0	40.0
70-71	36.04464498235228	37.0	37.0	38.5	33.0	40.0
72-73	35.83990655523621	37.0	35.0	37.0	33.0	40.0
74-75	35.663381183018366	37.0	33.0	37.0	33.0	40.0
76-77	33.87219029116872	35.0	33.0	37.0	27.0	37.0
78-79	35.10362982914594	37.0	33.0	37.0	33.0	37.0
80-81	35.167612505639724	37.0	33.0	37.0	33.0	37.0
82-83	35.048207933705115	37.0	33.0	37.0	33.0	37.0
84-85	34.889321445359954	37.0	33.0	37.0	33.0	37.0
86-87	34.76344928943106	37.0	33.0	37.0	33.0	37.0
88-89	34.68683561578297	37.0	33.0	37.0	33.0	37.0
90-91	34.60042886977611	37.0	33.0	37.0	33.0	37.0
92-93	34.531963624170785	37.0	33.0	37.0	33.0	37.0
94-95	34.523672977533636	37.0	33.0	37.0	33.0	37.0
96-97	34.52378625854437	37.0	33.0	37.0	33.0	37.0
98-99	34.52946930368056	37.0	33.0	37.0	33.0	37.0
100	34.53740248084281	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	6.0
20	53.0
21	273.0
22	1052.0
23	2784.0
24	6368.0
25	12396.0
26	21566.0
27	34655.0
28	51718.0
29	71981.0
30	97847.0
31	130616.0
32	173013.0
33	229777.0
34	313832.0
35	448360.0
36	757469.0
37	1737293.0
38	2200371.0
39	72386.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.46888542870248	20.10072882336848	12.951656858876722	24.478728889052313
2	14.840884066627288	21.81869094642698	40.511680171921924	22.828744815023807
3	18.046144932783285	26.678807175376924	30.645733324596442	24.629314567243348
4	12.102371233374173	17.867997188480693	39.54230942143389	30.487322156711244
5	12.789207235466629	38.286129759504476	36.08788999184295	12.836773013185951
6	29.55004191668048	38.5311640894652	18.955720742982002	12.963073250872315
7	25.920158948581033	32.90550846904979	23.887700137291773	17.286632445077398
8	24.434038743088074	36.71064497591776	21.708081263782013	17.147235017212147
9	25.74578252461297	16.08235557925202	21.304942833854952	36.866919062280054
10-11	23.906210592066753	26.808221599770576	29.630523003429595	19.655044804733077
12-13	24.742119569402064	24.496048117147577	29.558666344427436	21.203165969022923
14-15	21.755057602602154	25.83978290649519	27.066190543615377	25.33896894728728
16-17	21.383786999598826	28.941583662263177	27.78051984193768	21.89410949620032
18-19	21.427235752619612	28.175879923674763	29.418828536763854	20.97805578694177
20-21	22.157348935398538	27.48531413498204	29.275683802978254	21.08165312664117
22-23	21.852884249090696	27.414596819920817	29.152056415162896	21.58046251582559
24-25	21.611141639903327	27.553664482006734	29.250419997937733	21.584773880152206
26-27	21.595487883841358	27.70597406795816	29.14945060059555	21.54908744760493
28-29	21.567027002492033	27.777091206559284	29.142370633135002	21.513511157813685
30-31	21.797087580395296	27.662984063444355	29.04902153395709	21.490906822203257
32-33	21.59217469282419	27.8414023660972	28.99341971706607	21.57300322401253
34-35	21.744578320123757	27.834470088101433	29.003424085472325	21.41752750630249
36-37	21.71737305992028	27.773937398898347	28.936722268581683	21.571967272599693
38-39	21.541415481017566	27.92617621650468	29.010622548135462	21.521785754342297
40-41	21.808758312532188	27.82623675871625	28.838130253828286	21.52687467492328
42-43	21.722990297364248	27.888599758256294	28.68369862184975	21.70471132252971
44-45	21.814581378518618	28.001143502615683	28.504038915130565	21.68023620373514
46-47	21.896140920204747	27.999709106481173	28.24284843330757	21.861301540006504
48-49	21.80533866465124	28.13590549537753	28.190981001540393	21.86777483843084
50-51	21.815194505361912	28.252247308630217	28.121394084732753	21.811164101275114
52-53	22.000141316568754	28.12877391551225	28.039043846313895	21.8320409216051
54-55	21.944539294656284	28.048437692671936	28.046654070406085	21.96036894226569
56-57	21.919955245863022	28.212605690885933	27.951023506826584	21.916415556424468
58-59	21.927449734251184	28.21315907594082	27.9672924803083	21.8920987094997
60-61	21.975577934916977	28.19765117322187	27.990909509745826	21.83586138211533
62-63	21.930025125829637	28.270585885018544	27.957702566420206	21.841686422731613
64-65	21.91287675900169	28.184223561266116	27.947110074822696	21.955789604909494
66-67	21.98691077687367	28.215650148604045	27.825948443464853	21.971490631057428
68-69	21.962493282687475	28.24231011248341	27.8567616353039	21.93843496952522
70-71	21.958944093026787	28.03483711645412	27.928100564019687	22.078118226499406
72-73	22.018539102635913	28.071341166872486	27.874546431659862	22.035573298831732
74-75	21.87095816012151	28.212586839207077	27.84450864940092	22.071946351270494
76-77	22.010560295059676	28.139520537621998	27.745838371285025	22.104080796033305
78-79	21.94244206465784	28.19220194078303	27.727079569775633	22.138276424783502
80-81	21.945001044689132	28.198898991914263	27.738253923868083	22.117846039528523
82-83	21.989613136023994	28.169509009004535	27.802748961263912	22.038128893707558
84-85	22.07674661222743	28.130949766775178	27.755403102103326	22.03690051889407
86-87	21.93401218331799	28.16517163369291	27.827954796520025	22.072861386469075
88-89	21.97084646917616	28.18651563642901	27.759856726884696	22.082781167510138
90-91	22.035120267284324	28.185557389048093	27.779450290923208	21.999872052744372
92-93	22.050677426464617	28.161505579760764	27.775863894014773	22.011953099759847
94-95	21.918269307951814	28.225844770535325	27.84444967126518	22.01143625024768
96-97	21.9922250165465	28.3372077610681	27.881810211315205	21.788757011070192
98-99	23.05592442654748	30.24577261896015	28.096321027080208	18.60198192741216
100	21.965477636449233	31.145812398307392	22.026170961960425	24.862539003282947
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1754.0
1	1529.5
2	2009.0
3	2940.5
4	3962.0
5	6279.0
6	8362.5
7	9790.5
8	10779.5
9	10755.5
10	9737.5
11	8629.0
12	9209.0
13	11832.0
14	17557.5
15	25276.5
16	31586.5
17	35323.0
18	36089.0
19	34453.0
20	32077.5
21	30051.0
22	29940.0
23	32849.0
24	39037.5
25	48839.5
26	61727.0
27	75753.5
28	90218.5
29	105666.5
30	120437.5
31	133978.0
32	148063.5
33	161805.5
34	174627.0
35	186551.0
36	199956.0
37	212510.0
38	222001.5
39	230468.0
40	238936.0
41	245713.5
42	249639.5
43	254240.5
44	258158.5
45	262349.0
46	264995.5
47	264153.0
48	262491.0
49	257363.5
50	250763.0
51	242923.0
52	232024.5
53	219614.5
54	206713.5
55	191971.0
56	174658.5
57	156392.5
58	138397.0
59	120234.0
60	100559.0
61	81878.0
62	65282.0
63	50645.5
64	38255.5
65	28938.0
66	21958.5
67	16754.0
68	12860.0
69	9361.0
70	6638.5
71	4814.5
72	3472.5
73	2445.0
74	1738.0
75	1215.5
76	810.0
77	536.5
78	366.0
79	252.0
80	175.5
81	123.0
82	85.0
83	57.5
84	40.0
85	29.0
86	21.0
87	14.5
88	8.5
89	6.5
90	5.0
91	4.0
92	3.5
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	6.285533890891617E-5
7	0.0
8	0.0
9	0.0
10-11	0.00147710046435953
12-13	2.6713519036289374E-4
14-15	0.007511212999615483
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	147.0
20-21	413.0
22-23	786.0
24-25	1974.0
26-27	3045.0
28-29	6961.0
30-31	14009.0
32-33	18161.0
34-35	24979.0
36-37	36754.0
38-39	43501.0
40-41	43690.0
42-43	50723.0
44-45	60156.0
46-47	59654.0
48-49	56116.0
50-51	50320.0
52-53	48949.0
54-55	52109.0
56-57	56242.0
58-59	56053.0
60-61	54665.0
62-63	52093.0
64-65	54959.0
66-67	59253.0
68-69	60122.0
70-71	59338.0
72-73	55990.0
74-75	57193.0
76-77	56666.0
78-79	60860.0
80-81	61734.0
82-83	58754.0
84-85	58786.0
86-87	61445.0
88-89	66944.0
90-91	68510.0
92-93	71840.0
94-95	83225.0
96-97	211062.0
98-99	370141.0
100-101	3995497.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.72819103368208
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.00566049874351	79.83761969380186
2	6.651329223707898	11.670181615307177
3	1.4141820416208575	3.721908969111513
4	0.45284863264305997	1.589103654154083
5	0.19361190561513134	0.8492611121099733
6	0.10124982482441387	0.5329478384593815
7	0.056255058623938724	0.3454608170700316
8	0.032466984591480154	0.22786158612231863
9	0.022435829701760533	0.17714292786676855
>10	0.06845263649588774	0.8970377837716734
>50	0.0011663178365039304	0.06713631146159045
>100	3.154616707275015E-4	0.07170154174699517
>500	2.5583924793533357E-5	0.012636149016649669
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.5713834727229042E-5	0.0	0.0	0.0
3	0.0	3.1427669454458085E-5	0.0	0.0	0.0
4	0.0	7.856917363614522E-5	0.0	0.0	0.0
5	0.0	7.856917363614522E-5	0.0	0.0	0.0
6	0.0	7.856917363614522E-5	0.0	0.0	0.0
7	0.0	7.856917363614522E-5	0.0	0.0	0.0
8	0.0	7.856917363614522E-5	0.0	0.0	0.0
9	0.0	1.4142451254506137E-4	0.0	0.0	0.0
10-11	7.856917363614521E-6	1.4142451254506137E-4	0.0	2.3570752090843565E-5	0.0
12-13	1.5713834727229042E-5	1.4142451254506137E-4	0.0	4.714150418168713E-5	7.856917363614521E-6
14-15	3.1427669454458085E-5	2.199936861812066E-4	0.0	8.642609099975975E-5	1.5713834727229042E-5
16-17	4.714150418168713E-5	2.199936861812066E-4	0.0	9.428300836337426E-5	4.714150418168713E-5
18-19	4.714150418168713E-5	2.4356443827205015E-4	0.0	1.0999684309060331E-4	4.714150418168713E-5
20-21	4.714150418168713E-5	2.8284902509012274E-4	0.0	1.5713834727229043E-4	6.285533890891617E-5
22-23	4.714150418168713E-5	3.299905292718099E-4	0.0	1.5713834727229043E-4	6.285533890891617E-5
24-25	4.714150418168713E-5	3.299905292718099E-4	0.0	2.1213676881759209E-4	6.285533890891617E-5
26-27	4.714150418168713E-5	3.299905292718099E-4	0.0	2.592782729992792E-4	6.285533890891617E-5
28-29	4.714150418168713E-5	3.3784744663542445E-4	0.0	5.26413463362173E-4	6.285533890891617E-5
30-31	4.714150418168713E-5	3.4570436399903893E-4	0.0	0.0014928142990867591	6.285533890891617E-5
32-33	6.285533890891617E-5	3.4570436399903893E-4	0.0	0.0029463440113554453	6.285533890891617E-5
34-35	7.856917363614522E-5	3.61418198726268E-4	0.0	0.004934144104349919	6.285533890891617E-5
36-37	7.856917363614522E-5	3.7713203345349705E-4	0.0	0.0076526375121605435	6.285533890891617E-5
38-39	7.856917363614522E-5	3.7713203345349705E-4	0.0	0.011753948375967323	7.071225627253069E-5
40-41	1.099968430906033E-4	4.085597029079551E-4	0.0	0.015721691644592656	7.856917363614522E-5
42-43	1.099968430906033E-4	4.321304549987987E-4	0.0	0.01975229025212691	7.856917363614522E-5
44-45	1.2571067781783234E-4	4.871288765441003E-4	0.0	0.024230733149387184	9.428300836337426E-5
46-47	1.4142451254506137E-4	5.185565459985584E-4	0.0	0.029267017179464092	9.428300836337426E-5
48-49	1.4142451254506137E-4	5.185565459985584E-4	0.0	0.034028309101814494	9.428300836337426E-5
50-51	1.4142451254506137E-4	5.656980501802455E-4	0.0	0.03876603027207405	9.428300836337426E-5
52-53	1.4142451254506137E-4	5.656980501802455E-4	0.0	0.04349589452496999	9.428300836337426E-5
54-55	1.4142451254506137E-4	5.971257196347036E-4	0.0	0.048068620430593643	9.428300836337426E-5
56-57	1.4142451254506137E-4	6.285533890891617E-4	0.0	0.053136332130125	9.428300836337426E-5
58-59	1.4142451254506137E-4	6.442672238163908E-4	0.0	0.05841618059847396	9.428300836337426E-5
60-61	1.4142451254506137E-4	6.599810585436198E-4	0.0	0.06364103064527762	9.428300836337426E-5
62-63	1.4142451254506137E-4	6.599810585436198E-4	0.0	0.06875588384899067	1.099968430906033E-4
64-65	1.4142451254506137E-4	7.22836397452536E-4	0.0	0.07369788487070422	1.099968430906033E-4
66-67	1.4142451254506137E-4	7.22836397452536E-4	0.0	0.07911130093423462	1.099968430906033E-4
68-69	1.4142451254506137E-4	7.306933148161505E-4	0.0	0.0846818553450373	1.099968430906033E-4
70-71	1.4142451254506137E-4	7.464071495433796E-4	0.0	0.08912101365547952	1.099968430906033E-4
72-73	1.4142451254506137E-4	7.542640669069941E-4	0.0	0.09340303361864943	1.099968430906033E-4
74-75	1.5713834727229043E-4	7.69977901634223E-4	0.0	0.09681293575445814	1.099968430906033E-4
76-77	1.7285218199951946E-4	8.092624884522957E-4	0.0	0.1006863960147201	1.099968430906033E-4
78-79	1.7285218199951946E-4	8.249763231795248E-4	0.0	0.10316918190162228	1.1785376045421781E-4
80-81	1.7285218199951946E-4	8.328332405431393E-4	0.0	0.10383701987752952	1.2571067781783234E-4
82-83	1.7285218199951946E-4	8.485470752703683E-4	0.0	0.1043712902582553	1.4142451254506137E-4
84-85	1.7285218199951946E-4	8.485470752703683E-4	0.0	0.10466985311807266	1.4142451254506137E-4
86-87	1.7285218199951946E-4	8.721178273612118E-4	0.0	0.1048034207132541	1.492814299086759E-4
88	1.7285218199951946E-4	9.742577530882007E-4	0.0	0.10481127763061772	1.5713834727229043E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7340	0.0	49.066284	1
GTATCAA	12770	0.0	35.401794	1
TCAACGC	14830	0.0	29.488928	4
ATCAACG	15150	0.0	28.893896	3
CAACGCA	15260	0.0	28.686518	5
AACGCAG	15805	0.0	27.64396	6
TATCAAC	16645	0.0	26.653442	2
ACGCAGA	18445	0.0	23.733244	7
GTACATG	17380	0.0	23.439522	1
CGCAGAG	18740	0.0	23.359642	8
TACATGG	17365	0.0	22.92549	2
ACATGGG	17510	0.0	22.253954	3
CATGGGG	12715	0.0	20.198648	4
GCAGAGT	22935	0.0	19.089073	9
GAGTACT	14330	0.0	18.89563	12-13
GTGGTAT	3825	0.0	18.74299	1
CAGAGTA	21915	0.0	17.995186	10-11
AGAGTAC	20210	0.0	17.415913	10-11
GAGTACA	12275	0.0	16.834335	1
ATGGGGG	7655	0.0	16.748018	5
>>END_MODULE