FastQCFastQC Report
Fri 27 May 2016
SRR2139890_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139890_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7290395
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70950.044.785581
GTATCAA121950.032.3931161
TCAACGC138650.027.571624
ATCAACG140300.027.3354453
CAACGCA143200.026.666865
AACGCAG148350.025.7967076
TATCAAC152200.025.4151122
GTACATG178950.023.2236541
TACATGG181650.022.494072
ACGCAGA172350.022.1329277
CGCAGAG175150.021.802578
ACATGGG184350.021.6287173
CATGGGG142500.020.4229074
GCAGAGT216500.017.600229
ATGGGGG93750.017.2313965
GAGTACT130600.017.02730212-13
GAGTACA127450.016.8361111
CAGAGTA208700.016.55415510-11
AGAGTAC190600.016.40091510-11
AGTACAT136100.015.1320112
Produced by FastQC (version 0.11.5)