##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139890_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7290395
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.374355847659835	33.0	31.0	34.0	31.0	34.0
2	32.53720367689268	34.0	31.0	34.0	31.0	34.0
3	32.66123673682976	34.0	31.0	34.0	31.0	34.0
4	36.17328580961662	37.0	35.0	37.0	35.0	37.0
5	36.00982607938253	37.0	35.0	37.0	35.0	37.0
6	36.05231431218748	37.0	35.0	37.0	35.0	37.0
7	36.03043168442862	37.0	35.0	37.0	35.0	37.0
8	36.017790394073295	37.0	35.0	37.0	35.0	37.0
9	37.86772595997885	39.0	38.0	39.0	35.0	39.0
10-11	37.746012184524986	39.0	37.5	39.0	35.0	39.0
12-13	37.763260769821116	39.0	37.5	39.0	35.0	39.0
14-15	39.098896081762376	40.0	38.5	41.0	36.0	41.0
16-17	39.04904713667778	40.0	38.0	41.0	36.0	41.0
18-19	39.10692232725387	40.0	38.5	41.0	36.0	41.0
20-21	39.10424511912835	40.0	39.0	41.0	36.0	41.0
22-23	39.06688132069277	40.0	39.0	41.0	36.0	41.0
24-25	39.05223398113062	40.0	39.0	41.0	36.0	41.0
26-27	38.955121079914946	40.0	38.0	41.0	35.0	41.0
28-29	38.875761542783025	40.0	38.0	41.0	35.0	41.0
30-31	38.73480637863172	40.0	38.0	41.0	35.0	41.0
32-33	38.651468301800065	40.0	38.0	41.0	35.0	41.0
34-35	38.61124831712755	40.0	38.0	41.0	35.0	41.0
36-37	38.51344367983381	40.0	38.0	41.0	34.5	41.0
38-39	38.47598631665383	40.0	38.0	41.0	34.0	41.0
40-41	38.47706705028435	40.0	38.0	41.0	34.0	41.0
42-43	38.43982679256924	40.0	38.0	41.0	34.0	41.0
44-45	38.33472807779469	40.0	38.0	41.0	34.0	41.0
46-47	38.27763684877898	40.0	38.0	41.0	34.0	41.0
48-49	38.255057822032065	40.0	38.0	41.0	34.0	41.0
50-51	38.174746022179896	40.0	37.5	41.0	33.5	41.0
52-53	38.04539576350284	40.0	37.0	41.0	33.0	41.0
54-55	37.86891666255455	40.0	37.0	41.0	33.0	41.0
56-57	37.74288286655599	39.0	36.5	41.0	33.0	41.0
58-59	37.521415894777604	39.0	36.0	41.0	32.5	41.0
60-61	37.355441403348934	39.0	36.0	40.5	32.0	41.0
62-63	37.140927576159875	39.0	35.0	40.0	32.0	41.0
64-65	36.82621557888574	38.5	35.0	40.0	31.0	41.0
66-67	36.75254121077005	38.0	35.0	40.0	32.0	41.0
68-69	36.517799441416784	37.5	35.0	40.0	31.5	41.0
70-71	36.200199734937904	37.0	35.0	39.5	31.0	41.0
72-73	35.888673730418276	36.5	34.5	39.0	31.0	41.0
74-75	35.491690720792235	36.0	34.0	39.0	31.0	40.0
76-77	33.44976038375781	34.5	32.0	36.5	27.5	39.0
78-79	34.63910693851542	35.0	34.0	37.0	30.0	39.0
80-81	34.64799020634425	35.0	34.0	37.0	30.0	39.0
82-83	34.36682938852696	35.0	34.0	36.0	30.0	38.5
84-85	34.11460269486088	35.0	34.0	36.0	30.0	37.0
86-87	33.912095965750396	35.0	34.0	36.0	30.0	37.0
88-89	33.67727224957373	35.0	34.0	35.0	30.0	36.5
90-91	33.5199957686211	35.0	33.0	35.0	30.0	36.0
92-93	33.45656450242285	35.0	33.0	35.0	30.0	36.0
94-95	33.42644450266579	35.0	33.0	35.0	30.0	36.0
96-97	33.45952781192188	35.0	33.0	35.0	30.0	35.5
98-99	33.48702193984323	35.0	33.0	35.0	30.0	35.0
100-101	32.23792063240297	34.0	31.0	34.5	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	9.0
20	31.0
21	137.0
22	620.0
23	2017.0
24	5377.0
25	11699.0
26	21571.0
27	35766.0
28	53060.0
29	74825.0
30	99447.0
31	130345.0
32	172198.0
33	234294.0
34	340437.0
35	522220.0
36	926925.0
37	1711406.0
38	2045871.0
39	888438.0
40	13701.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.19955160179935	20.10419188535052	13.022010467196907	24.67424604565322
2	14.954560587278227	21.778545156903867	40.21133226072789	23.055561995090013
3	18.22409677622008	26.294725683934978	30.59078078993348	24.89039674991146
4	12.360914524337584	17.875913651270555	39.0395933496571	30.723578474734758
5	12.85298689741058	38.11293523214324	36.08596575958683	12.94811211085935
6	29.937129606832002	38.27414015289981	18.818033865106074	12.970696375162113
7	26.199194775809037	32.53214641956153	23.822902801385716	17.44575600324372
8	24.51277789947576	36.66073600470539	21.68349413698444	17.14299195883441
9	25.69773083252279	16.080760091753753	21.21114898901745	37.01036008670601
10-11	24.041521215555786	26.618933532188628	29.55738260218739	19.7821626500682
12-13	24.78852080918477	24.22066246391046	29.48445926886198	21.506357458042793
14-15	21.87674094683641	25.68416231579269	26.73086164690562	25.70823509046528
16-17	21.71485954462044	28.774060203902195	27.473403912400464	22.0376763390769
18-19	21.718287340613873	28.119464890301526	28.91925716487438	21.242990604210227
20-21	22.36382063594322	27.45228958091867	28.729000375234882	21.454889407903224
22-23	22.091110629436248	27.423052440673967	28.60101956545001	21.884817364439773
24-25	21.942060446553356	27.476101512227284	28.699420041865665	21.882417999353695
26-27	21.882266456678646	27.698005742982485	28.586400587270923	21.833327213067946
28-29	21.846712129800274	27.71826147720401	28.60001334093304	21.83501305206268
30-31	22.07943226462618	27.619322093886932	28.489025154360387	21.8122204871265
32-33	21.79062551190464	27.771536320264396	28.537545935024816	21.90029223280615
34-35	21.937492271714152	27.777680301298947	28.502535432565207	21.782291994421694
36-37	21.952640382751344	27.75966107301292	28.43774533441545	21.849953209820285
38-39	21.80996251879394	27.842274330658295	28.50543910875971	21.84232404178805
40-41	22.037422155677767	27.697736327957106	28.413334926949503	21.851506589415624
42-43	21.942693650884607	27.778907553647958	28.2891808785051	21.989217916962335
44-45	22.000300258139198	27.922910211703776	28.0964556393294	21.980333890827623
46-47	22.12641155706634	27.848199528246663	27.86317313827073	22.162215776416264
48-49	22.025409175189335	27.95119575701863	27.896393611916743	22.12700145587529
50-51	21.998340552989067	28.044971345946117	27.863628702729788	22.09305939833503
52-53	22.143640583040817	27.98504362276693	27.794911001467636	22.07640479272462
54-55	22.09736579014025	27.91199574108212	27.75619917819185	22.23443929058579
56-57	22.131945665046263	28.07167872323847	27.664179856265232	22.132195755450038
58-59	22.10094514928836	28.11327411989881	27.70791716538196	22.077863565430867
60-61	22.154412333988148	28.113168264523015	27.678808609850936	22.0536107916379
62-63	22.138857338709062	28.20123454964355	27.6169418317248	22.042966279922585
64-65	22.1056201868446	28.170242262240723	27.564806718593815	22.159330832320858
66-67	22.162562131919096	28.14477263328975	27.514440662063627	22.17822457272753
68-69	22.123495634602754	28.110358743511455	27.597403118830382	22.168742503055412
70-71	22.131297112177325	27.918830454487363	27.609939734639738	22.33993269869557
72-73	22.209334903441395	27.946739108244067	27.596201334649766	22.247724653664772
74-75	22.021490662205263	28.077589160618977	27.569379859322936	22.331540317852824
76-77	22.173351976016157	27.995786130514716	27.53599235359827	22.294869539870852
78-79	22.09098507043774	28.023449076194396	27.526035113392933	22.35953073997493
80-81	22.117340352858868	28.047698131043987	27.511444619308723	22.323516896788423
82-83	22.10184940472428	28.03634184593423	27.61777907776366	22.244029671577827
84-85	22.25689008903534	27.97347823213392	27.53407985799666	22.235551820834083
86-87	22.076031457593665	28.03828334132148	27.632276637407443	22.25340856367741
88-89	22.146636505865036	28.011750496849864	27.558113003412444	22.283499993872656
90-91	22.18292677015751	28.015877859816595	27.579937069023764	22.22125830100213
92-93	22.180867064336592	27.94303062176564	27.63664383547853	22.239458478419234
94-95	22.084227979903595	27.977494174843486	27.68348345897517	22.25479438627775
96-97	22.065829941778706	28.01354463994003	27.718525739309673	22.20209967897159
98-99	22.338327305697774	28.620462220952238	27.703818190510333	21.337392282839655
100-101	26.357603182107397	35.55241483988156	28.422304610714377	9.667677367296665
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1809.0
1	1540.5
2	1930.0
3	2789.0
4	3761.5
5	6075.5
6	8063.0
7	9307.5
8	10087.5
9	9876.0
10	8986.0
11	8002.5
12	8498.5
13	11121.0
14	16807.0
15	24289.0
16	30355.5
17	33895.5
18	35101.0
19	34732.5
20	33818.5
21	33272.0
22	34546.0
23	39035.5
24	47063.5
25	58682.0
26	73500.5
27	89180.5
28	106142.0
29	124054.0
30	139855.0
31	155272.5
32	171189.0
33	185882.5
34	200375.0
35	214382.5
36	229704.0
37	243896.0
38	255672.0
39	267153.0
40	278281.0
41	288941.0
42	295924.5
43	301576.0
44	306840.0
45	311931.0
46	314472.0
47	314492.5
48	316117.5
49	313628.5
50	306589.0
51	297913.0
52	286367.5
53	273496.0
54	258068.0
55	239523.0
56	220677.5
57	200630.0
58	178621.0
59	155474.0
60	131277.0
61	108218.0
62	87374.0
63	68516.0
64	52029.5
65	39730.5
66	30779.5
67	23771.5
68	18369.5
69	13610.5
70	9852.0
71	7222.5
72	5286.5
73	3683.0
74	2537.0
75	1770.0
76	1258.0
77	888.5
78	635.0
79	429.5
80	263.5
81	164.0
82	124.0
83	91.5
84	63.0
85	45.5
86	33.0
87	27.0
88	21.0
89	15.5
90	12.5
91	9.0
92	7.5
93	6.0
94	4.5
95	3.5
96	3.0
97	2.5
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.00491057068924249
3	1.2345010112620782E-4
4	5.486671161164793E-5
5	5.486671161164793E-5
6	0.0
7	4.115003370873595E-5
8	4.6636704869900737E-4
9	1.646001348349438E-4
10-11	2.0575016854367974E-5
12-13	2.811918970096956E-4
14-15	2.0575016854367974E-5
16-17	5.075170824077434E-4
18-19	2.7433355805823965E-5
20-21	6.172750598242412E-5
22-23	0.0
24-25	0.0
26-27	7.550228236535746E-5
28-29	6.869686984086507E-6
30-31	9.636437186157588E-5
32-33	5.041328883244135E-4
34-35	0.0
36-37	4.1172397271205165E-4
38-39	0.0012040369457680636
40-41	0.0
42-43	0.0
44-45	3.268401572958204E-4
46-47	2.2074113098925874E-5
48-49	5.2171197548848074E-5
50-51	2.3387314524450268E-4
52-53	6.332881587780072E-4
54-55	0.003310769934597245
56-57	0.0037824742805425225
58-59	0.004402331271944354
60-61	0.0010911222681928793
62-63	8.12567163754629E-6
64-65	6.663399133165811E-4
66-67	0.0
68-69	0.0
70-71	1.7142022081495743E-5
72-73	2.2587265247728873E-4
74-75	7.040977343983164E-5
76-77	8.919374120382474E-6
78-79	9.04224537037037E-6
80-81	5.871588721632199E-4
82-83	0.0
84-85	0.0
86-87	2.8734335835998205E-5
88-89	0.0
90-91	0.0012760527435133986
92-93	1.81210230646395E-4
94-95	1.0252848574551588E-5
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	199.0
20-21	577.0
22-23	1251.0
24-25	2898.0
26-27	4874.0
28-29	11231.0
30-31	22697.0
32-33	28401.0
34-35	39130.0
36-37	60282.0
38-39	69809.0
40-41	68208.0
42-43	75442.0
44-45	87588.0
46-47	88057.0
48-49	82867.0
50-51	75745.0
52-53	72940.0
54-55	79594.0
56-57	83130.0
58-59	82721.0
60-61	80687.0
62-63	75240.0
64-65	77477.0
66-67	83489.0
68-69	82158.0
70-71	79490.0
72-73	74615.0
74-75	75543.0
76-77	74619.0
78-79	79462.0
80-81	79838.0
82-83	75792.0
84-85	75033.0
86-87	77840.0
88-89	83464.0
90-91	89770.0
92-93	88204.0
94-95	97218.0
96-97	145475.0
98-99	577535.0
100-101	4079805.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.42362110263159
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.97493279603917	79.533580545983
2	6.517192657134732	11.39513163020399
3	1.4915118349629641	3.9118009658967883
4	0.4969641822761228	1.737856334915476
5	0.21380133923492145	0.9345643636254486
6	0.11020090111924936	0.5780497094770696
7	0.061758962419061675	0.3779434491150998
8	0.033537956082545145	0.23456076520936997
9	0.023263998530357554	0.1830440693565141
>10	0.07548531743200428	1.010634475103368
>50	0.001158606405563042	0.06255652334307467
>100	1.6863362068819567E-4	0.029406884376465308
>500	2.281474259063902E-5	0.010870283394299629
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.3716677902911983E-5	0.0	2.7433355805823965E-5	0.0
3	0.0	1.3716677902911983E-5	0.0	2.7433355805823965E-5	0.0
4	0.0	1.2345010112620785E-4	0.0	2.7433355805823965E-5	0.0
5	0.0	1.2345010112620785E-4	0.0	4.115003370873595E-5	0.0
6	0.0	1.371667790291198E-4	0.0	4.115003370873595E-5	0.0
7	0.0	1.371667790291198E-4	0.0	4.115003370873595E-5	0.0
8	0.0	1.371667790291198E-4	0.0	4.115003370873595E-5	1.3716677902911983E-5
9	0.0	2.3318352434950368E-4	0.0	5.486671161164793E-5	2.7433355805823965E-5
10-11	0.0	2.3318352434950368E-4	0.0	5.486671161164793E-5	2.7433355805823965E-5
12-13	0.0	2.3318352434950368E-4	0.0	5.486671161164793E-5	2.7433355805823965E-5
14-15	0.0	3.3605860862134356E-4	0.0	5.486671161164793E-5	6.172505056310391E-5
16-17	6.858338951455991E-6	3.703503033786235E-4	0.0	5.486671161164793E-5	8.23000674174719E-5
18-19	1.3716677902911983E-5	3.9092532023299147E-4	0.0	5.486671161164793E-5	8.23000674174719E-5
20-21	1.3716677902911983E-5	4.1150033708735946E-4	0.0	7.54417284660159E-5	8.23000674174719E-5
22-23	1.3716677902911983E-5	4.869420655533754E-4	0.0	9.601674532038387E-5	1.2345010112620782E-4
24-25	1.3716677902911983E-5	5.075170824077434E-4	0.0	2.1260850749513574E-4	1.508834569320318E-4
26-27	1.3716677902911983E-5	5.075170824077434E-4	0.0	3.429169475727996E-4	1.508834569320318E-4
28-29	1.3716677902911983E-5	5.075170824077434E-4	0.0	0.0010561841985242226	1.508834569320318E-4
30-31	1.3716677902911983E-5	5.623837940193913E-4	0.0	0.0034291694757279958	1.508834569320318E-4
32-33	1.3716677902911983E-5	5.623837940193913E-4	0.0	0.007845939760465654	1.508834569320318E-4
34-35	1.3716677902911983E-5	5.898171498252152E-4	0.0	0.013051419024620751	1.646001348349438E-4
36-37	1.3716677902911983E-5	6.241088445824951E-4	0.0	0.020602450210173796	1.646001348349438E-4
38-39	1.3716677902911983E-5	6.515422003883192E-4	0.0	0.03392134445390133	1.646001348349438E-4
40-41	1.3716677902911983E-5	7.68133962563071E-4	0.0	0.044956411826794024	1.9203349064076774E-4
42-43	1.3716677902911983E-5	7.955673183688949E-4	0.0	0.05385167744683245	1.9203349064076774E-4
44-45	1.3716677902911983E-5	8.572923689319988E-4	0.0	0.06432436102570574	1.9203349064076774E-4
46-47	1.3716677902911983E-5	9.053007415921908E-4	0.0	0.07592867053156928	1.9889182959222372E-4
48-49	1.3716677902911983E-5	9.053007415921908E-4	0.0	0.08696373790446196	2.3318352434950368E-4
50-51	1.3716677902911983E-5	0.0010150341648154867	0.0	0.09726496300954887	2.3318352434950368E-4
52-53	1.3716677902911983E-5	0.0010150341648154867	0.0	0.10624938703595621	2.4690020225241565E-4
54-55	1.3716677902911983E-5	0.0010218925037669426	0.0	0.11561101970469365	2.743335580582396E-4
56-57	1.3716677902911983E-5	0.0010424675206213106	0.0	0.12477376054383885	2.8805023596115164E-4
58-59	1.3716677902911983E-5	0.0010561841985242226	0.0	0.13298319226873168	3.017669138640636E-4
60-61	1.3716677902911983E-5	0.0010973342322329585	0.0	0.14102802385878954	3.017669138640636E-4
62-63	1.3716677902911983E-5	0.0011247675880387826	0.0	0.14842131324845909	3.1548359176697557E-4
64-65	1.3716677902911983E-5	0.0012482176891649903	0.0	0.15522478548830343	3.2234193071843155E-4
66-67	1.3716677902911983E-5	0.0012619343670679023	0.0	0.16274152497909922	3.3605860862134356E-4
68-69	1.3716677902911983E-5	0.0013168010786795503	0.0	0.17022397277513768	3.4291694757279954E-4
70-71	1.3716677902911983E-5	0.0013373760955339184	0.0	0.17729492023408883	3.5663362547571156E-4
72-73	1.3716677902911983E-5	0.0013579511123882862	0.0	0.18337140854507883	3.977836591844475E-4
74-75	1.3716677902911983E-5	0.0014333928408543021	0.0	0.1882202541837582	3.977836591844475E-4
76-77	1.3716677902911983E-5	0.0016185679925436137	0.0	0.19263016612954442	3.977836591844475E-4
78-79	1.3716677902911983E-5	0.0016871513820581737	0.0	0.1964845526202627	3.977836591844475E-4
80-81	1.3716677902911983E-5	0.0016940097210096298	0.0	0.19841174586562182	4.1150033708735946E-4
82-83	1.3716677902911983E-5	0.0017145847378639977	0.0	0.19911129643867032	4.3893369289318344E-4
84-85	1.3716677902911983E-5	0.001735159754718366	0.0	0.19963253019898097	4.3893369289318344E-4
86-87	1.3716677902911983E-5	0.0017420180936698218	0.0	0.19994801379074795	4.3893369289318344E-4
88-89	1.3716677902911983E-5	0.0018243181610872936	0.0	0.20004403053606834	4.457920318446394E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7095	0.0	44.78558	1
GTATCAA	12195	0.0	32.393116	1
TCAACGC	13865	0.0	27.57162	4
ATCAACG	14030	0.0	27.335445	3
CAACGCA	14320	0.0	26.66686	5
AACGCAG	14835	0.0	25.796707	6
TATCAAC	15220	0.0	25.415112	2
GTACATG	17895	0.0	23.223654	1
TACATGG	18165	0.0	22.49407	2
ACGCAGA	17235	0.0	22.132927	7
CGCAGAG	17515	0.0	21.80257	8
ACATGGG	18435	0.0	21.628717	3
CATGGGG	14250	0.0	20.422907	4
GCAGAGT	21650	0.0	17.60022	9
ATGGGGG	9375	0.0	17.231396	5
GAGTACT	13060	0.0	17.027302	12-13
GAGTACA	12745	0.0	16.836111	1
CAGAGTA	20870	0.0	16.554155	10-11
AGAGTAC	19060	0.0	16.400915	10-11
AGTACAT	13610	0.0	15.132011	2
>>END_MODULE