FastQCFastQC Report
Fri 27 May 2016
SRR2139889_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139889_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9144155
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92850.041.9894261
GTATCAA155600.032.5650181
CTTATAC81050.028.3810541
TCAACGC181800.027.0232034
ATCAACG181100.027.0156523
CAACGCA184900.026.416415
AACGCAG191850.025.565876
TATCAAC198100.025.2099512
TATACAC119050.023.0017533
TACATGG243750.022.2028982
GTACATG253000.022.0004731
ACGCAGA227200.021.552377
ACATGGG246300.021.3959373
CGCAGAG228750.021.3885978
TTATACA120350.020.259412
CATGGGG178200.019.4873454
ACATCTC126150.019.3278398
ACACATC130400.018.2623486
TACACAT155000.017.8237725
GCAGAGT282250.017.4637879
Produced by FastQC (version 0.11.5)