##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139889_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9144155
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.3641139066431	33.0	31.0	34.0	31.0	34.0
2	32.525868163870804	34.0	31.0	34.0	31.0	34.0
3	32.64970934985244	34.0	31.0	34.0	31.0	34.0
4	36.164884344152085	37.0	35.0	37.0	35.0	37.0
5	35.99999650049676	37.0	35.0	37.0	35.0	37.0
6	36.043141547797475	37.0	35.0	37.0	35.0	37.0
7	36.02205365066537	37.0	35.0	37.0	35.0	37.0
8	36.00924809345423	37.0	35.0	37.0	35.0	37.0
9	37.862301218647325	39.0	38.0	39.0	35.0	39.0
10-11	37.736952949725804	39.0	37.0	39.0	35.0	39.0
12-13	37.7575102893597	39.0	37.0	39.0	35.0	39.0
14-15	39.08434201957425	40.0	38.5	41.0	36.0	41.0
16-17	39.03416860278506	40.0	38.0	41.0	36.0	41.0
18-19	39.09580114291589	40.0	38.5	41.0	36.0	41.0
20-21	39.09449890955726	40.0	39.0	41.0	36.0	41.0
22-23	39.056462268125216	40.0	39.0	41.0	36.0	41.0
24-25	39.040935526259695	40.0	39.0	41.0	36.0	41.0
26-27	38.947168937977416	40.0	38.0	41.0	35.0	41.0
28-29	38.87271498772138	40.0	38.0	41.0	35.0	41.0
30-31	38.735587434569766	40.0	38.0	41.0	35.0	41.0
32-33	38.65179439245679	40.0	38.0	41.0	35.0	41.0
34-35	38.603075001769255	40.0	38.0	41.0	35.0	41.0
36-37	38.51030264477438	40.0	38.0	41.0	34.5	41.0
38-39	38.47130809641655	40.0	38.0	41.0	34.0	41.0
40-41	38.485099412736346	40.0	38.0	41.0	34.0	41.0
42-43	38.452942145910754	40.0	38.0	41.0	34.0	41.0
44-45	38.34895188863963	40.0	38.0	41.0	34.0	41.0
46-47	38.295527788632015	40.0	38.0	41.0	34.0	41.0
48-49	38.27758483439436	40.0	38.0	41.0	34.0	41.0
50-51	38.19591363732168	40.0	38.0	41.0	33.5	41.0
52-53	38.075506940846566	40.0	37.0	41.0	33.0	41.0
54-55	37.89908921725384	40.0	37.0	41.0	33.0	41.0
56-57	37.77406139235878	39.5	37.0	41.0	33.0	41.0
58-59	37.559671603453026	39.0	36.0	41.0	32.5	41.0
60-61	37.38762502143626	39.0	36.0	40.5	32.0	41.0
62-63	37.177741145375734	39.0	35.0	40.0	32.0	41.0
64-65	36.87766940024022	38.5	35.0	40.0	31.0	41.0
66-67	36.808678446467205	38.0	35.0	40.0	32.0	41.0
68-69	36.577884518954306	37.5	35.0	40.0	31.5	41.0
70-71	36.26564290074849	37.0	35.0	39.5	31.0	41.0
72-73	35.95030280781262	36.5	35.0	39.0	31.0	41.0
74-75	35.55004867016332	36.0	34.0	39.0	31.0	40.0
76-77	33.50145097753556	34.5	32.0	36.5	27.5	39.0
78-79	34.689841871680194	35.0	34.0	37.0	30.0	39.0
80-81	34.68980683914565	35.0	34.0	37.0	31.0	39.0
82-83	34.40284611110327	35.0	34.0	36.5	30.0	39.0
84-85	34.14764259385258	35.0	34.0	36.0	30.0	37.0
86-87	33.93989553352529	35.0	34.0	36.0	30.0	37.0
88-89	33.70667300209791	35.0	34.0	35.0	30.0	36.5
90-91	33.542808569011115	35.0	33.5	35.0	30.0	36.0
92-93	33.47614861848301	35.0	33.0	35.0	30.0	36.0
94-95	33.4491649127025	35.0	33.0	35.0	30.0	36.0
96-97	33.48137171631768	35.0	33.0	35.0	30.0	35.5
98-99	33.50651176215478	35.0	33.0	35.0	30.0	35.0
100-101	32.25753484407055	34.0	31.0	34.5	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	9.0
20	42.0
21	187.0
22	763.0
23	2582.0
24	6759.0
25	14407.0
26	26554.0
27	43958.0
28	65067.0
29	90828.0
30	122364.0
31	160782.0
32	212156.0
33	288879.0
34	421120.0
35	642262.0
36	1140879.0
37	2121660.0
38	2603516.0
39	1161172.0
40	18206.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.863660447575526	20.388630770147707	13.066434241326835	24.68127454094993
2	14.78228763804734	21.88125738692233	40.59006574258344	22.74638923244689
3	18.05480294872904	26.65279840877379	30.739654880725293	24.552743761771882
4	12.196255289310074	18.26261399226675	39.21364596888218	30.32748474954099
5	12.696489012100631	38.244145163847925	36.26975716715059	12.78960865690086
6	29.37923733794976	38.53675927409367	19.097718706649218	12.98628468130735
7	25.890298228759246	32.62694037885404	23.87487963622664	17.607881756160083
8	24.281018997778027	36.723471099795034	21.947312164032805	17.04819773839413
9	25.579297423037296	16.072331374737715	21.241982704327373	37.10638849789761
10-11	23.88354588632668	26.714989724032336	29.68634266866328	19.715121720977706
12-13	24.713865491354778	24.284125131259128	29.532412392392672	21.469596984993427
14-15	21.787849373490427	25.72503700178278	26.780815371219692	25.706298253507104
16-17	21.646652055806626	28.89173540808202	27.452661748492456	22.008950787618893
18-19	21.68386800092518	28.199532925677662	28.87439572054498	21.242203352852176
20-21	22.31425701850459	27.465665307279487	28.62776007555363	21.59231759866229
22-23	22.01608514518582	27.58181903793303	28.513023413493276	21.889072403387875
24-25	21.85512839321968	27.69137379919056	28.65525972230747	21.798238085282293
26-27	21.831433285097425	27.80812177996304	28.582090850971397	21.778354083968132
28-29	21.6517770207346	27.774640523935574	28.68852813725947	21.885054318070356
30-31	21.982541032121055	27.639716788479696	28.395250557276153	21.982491622123096
32-33	21.900271016031546	27.778594908156595	28.55152983063799	21.769604245173866
34-35	21.953666902633227	28.00022880487219	28.489972011826087	21.556132280668493
36-37	21.85676841644886	27.857085937823694	28.362577289883127	21.92356835584432
38-39	21.74355921787823	27.814311992844814	28.561840905295877	21.88028788398108
40-41	21.92259162818262	27.70184892600588	28.517289382618355	21.858270063193146
42-43	21.966084416023868	27.75014147092322	28.362378669784547	21.92139544326836
44-45	21.903329333449133	27.99918551913211	28.20155170154117	21.89593344587759
46-47	22.13899838917478	27.77300529102094	27.861767825208457	22.226228494595823
48-49	22.002347943311694	27.887223604131982	27.917672350252616	22.192756102303708
50-51	21.860206495719844	27.988761360091885	28.027115255645008	22.123916888543263
52-53	22.017001656435596	27.9488434775163	28.026017408424668	22.008137457623437
54-55	21.994632229424973	27.892859264415844	27.88864553272446	22.223862973434727
56-57	22.13752460861472	28.065092191418195	27.652597110613208	22.14478608935388
58-59	21.931254624897566	28.240563926504365	27.878324636067852	21.949856812530218
60-61	22.18135610667299	28.300430995273874	27.60073224560133	21.91748065245181
62-63	22.025317608776326	28.44078979765267	27.53491943983147	21.99897315373953
64-65	21.977907344919654	28.335131413876496	27.59591308444899	22.091048156754862
66-67	22.092920261640863	28.21517862611423	27.51053501944401	22.181366092800893
68-69	22.107931737686766	28.128305598382653	27.614039843133963	22.14972282079662
70-71	22.098557181235915	27.881906064088124	27.67380599105654	22.34573076361942
72-73	22.128503075677127	27.93916836147396	27.653311812693083	22.27901675015583
74-75	21.98939429499337	28.069185906037557	27.635633312016587	22.30578648695248
76-77	22.11509992354569	27.99658419280464	27.561347309456018	22.32696857419365
78-79	22.09626678925719	28.018402225973315	27.510550513629667	22.374780471139825
80-81	22.07948827872806	28.013746544082846	27.580101810408014	22.326663366781077
82-83	22.083316665941332	27.988980935384493	27.67872651589962	22.248975882774555
84-85	22.251483588257287	27.94056999999146	27.534992874010438	22.27295353774081
86-87	22.070238574346444	28.0147898383168	27.644972970860593	22.269998616476162
88-89	22.163148160245104	27.980363544840785	27.553390805260214	22.3030974896539
90-91	22.210370746952535	27.976322577386277	27.56558096942026	22.247725706240924
92-93	22.222102367009533	27.94519522671637	27.608044357344742	22.22465804892936
94-95	22.065938009244473	27.983343475418067	27.688652288158316	22.262066227179147
96-97	22.055650531526908	27.98840028339804	27.71890074266225	22.237048442412796
98-99	22.315972901405917	28.529778995356597	27.75755503623025	21.396693067007234
100-101	26.365039308585477	35.560026419262506	28.40766581772237	9.667268454429651
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1815.0
1	1647.5
2	2317.0
3	3375.0
4	4581.5
5	7385.5
6	9748.5
7	11216.0
8	11970.5
9	11551.5
10	10481.5
11	9372.5
12	9949.0
13	13122.5
14	20253.5
15	29511.0
16	36898.0
17	41363.5
18	43239.5
19	42805.5
20	41276.0
21	40474.0
22	42082.0
23	47904.5
24	58412.5
25	73492.0
26	92674.0
27	113682.0
28	135590.5
29	157964.5
30	178594.0
31	197997.5
32	217892.0
33	237750.5
34	257784.0
35	277312.0
36	299243.0
37	320445.5
38	336230.5
39	348939.5
40	362108.0
41	375825.0
42	384761.0
43	392610.5
44	400974.0
45	406820.5
46	410228.0
47	412125.5
48	417723.0
49	415891.0
50	402943.5
51	389121.0
52	371214.0
53	349650.5
54	326635.0
55	301669.5
56	274660.0
57	245964.0
58	216906.5
59	186788.0
60	156337.0
61	127804.5
62	101777.5
63	79454.5
64	60241.5
65	45228.0
66	34388.5
67	26491.5
68	20454.0
69	14752.0
70	10382.5
71	7479.0
72	5246.5
73	3509.0
74	2345.5
75	1648.5
76	1164.5
77	798.5
78	546.5
79	372.5
80	263.0
81	187.5
82	141.0
83	100.5
84	65.5
85	45.0
86	32.0
87	24.5
88	17.5
89	12.0
90	8.0
91	7.0
92	7.0
93	5.0
94	2.5
95	1.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.005052407794924736
3	1.312313712967464E-4
4	4.37437904322488E-5
5	5.4679738040311E-5
6	0.0
7	0.0
8	3.608862710660526E-4
9	1.968470569451196E-4
10-11	4.37437904322488E-5
12-13	3.171424806338038E-4
14-15	1.09359476080622E-5
16-17	4.757137209507056E-4
18-19	0.0
20-21	8.202358976568705E-5
22-23	0.0
24-25	0.0
26-27	9.303490872263016E-5
28-29	1.643337662615109E-5
30-31	8.234992878378158E-5
32-33	5.345455282896924E-4
34-35	0.0
36-37	3.903479009125274E-4
38-39	0.0010534813021997873
40-41	0.0
42-43	0.0
44-45	3.2079851065500175E-4
46-47	5.9167130339744764E-6
48-49	4.19982528726805E-5
50-51	1.520047449801193E-4
52-53	4.5551925285978377E-4
54-55	0.0029794433988343393
56-57	0.0030334247384255727
58-59	0.00414860947523168
60-61	8.979258693223757E-4
62-63	6.600965496819358E-6
64-65	5.222474396317111E-4
66-67	0.0
68-69	0.0
70-71	1.401488661255986E-5
72-73	9.95271748287244E-5
74-75	4.3248560868096646E-5
76-77	0.0
78-79	0.0
80-81	4.518995144189084E-4
82-83	0.0
84-85	0.0
86-87	7.878836727575591E-6
88-89	0.0
90-91	0.0010509790847829715
92-93	1.4935781119778657E-4
94-95	8.455259585770068E-6
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	299.0
20-21	784.0
22-23	1576.0
24-25	3914.0
26-27	6696.0
28-29	15668.0
30-31	32936.0
32-33	40432.0
34-35	55706.0
36-37	85438.0
38-39	99232.0
40-41	95668.0
42-43	104422.0
44-45	119995.0
46-47	119261.0
48-49	112345.0
50-51	102892.0
52-53	99076.0
54-55	107517.0
56-57	113836.0
58-59	113600.0
60-61	111956.0
62-63	106818.0
64-65	109648.0
66-67	115551.0
68-69	107268.0
70-71	103920.0
72-73	97107.0
74-75	97574.0
76-77	96359.0
78-79	102242.0
80-81	102511.0
82-83	96854.0
84-85	94679.0
86-87	98738.0
88-89	104946.0
90-91	113786.0
92-93	110213.0
94-95	121091.0
96-97	179267.0
98-99	705215.0
100-101	4937119.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.91468910625849
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.30377571544513	77.58420815713765
2	6.779471402115843	11.649123556351059
3	1.6296371010233082	4.200292946713257
4	0.5958878625786569	2.047820818225528
5	0.27104512070214104	1.1643378639446378
6	0.14106701643368033	0.7271837316028262
7	0.08837046164583981	0.5314624516935207
8	0.05203829973018235	0.3576683474349529
9	0.034263471311030434	0.2649361936849548
>10	0.10297404332302697	1.348324553885997
>50	0.0012140950423372972	0.0684393017465386
>100	2.3558791806637067E-4	0.04421250880801795
>500	1.982273070658477E-5	0.011989568771088933
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.09359476080622E-5	0.0	0.0	0.0
3	0.0	1.09359476080622E-5	0.0	0.0	0.0
4	0.0	3.28078428241866E-5	0.0	0.0	0.0
5	0.0	3.28078428241866E-5	0.0	1.09359476080622E-5	0.0
6	0.0	3.28078428241866E-5	0.0	1.09359476080622E-5	0.0
7	0.0	3.28078428241866E-5	0.0	1.09359476080622E-5	0.0
8	0.0	6.56156856483732E-5	0.0	1.09359476080622E-5	0.0
9	0.0	9.84235284725598E-5	0.0	2.18718952161244E-5	1.09359476080622E-5
10-11	0.0	9.84235284725598E-5	0.0	3.82758166282177E-5	1.09359476080622E-5
12-13	0.0	9.84235284725598E-5	0.0	5.4679738040311E-5	1.09359476080622E-5
14-15	1.09359476080622E-5	1.4763529270883972E-4	0.0	5.4679738040311E-5	1.64039214120933E-5
16-17	1.09359476080622E-5	1.531032665128708E-4	0.0	5.4679738040311E-5	2.18718952161244E-5
18-19	1.09359476080622E-5	1.64039214120933E-4	0.0	7.65516332564354E-5	2.18718952161244E-5
20-21	1.09359476080622E-5	1.8044313553302628E-4	0.0	8.20196070604665E-5	2.18718952161244E-5
22-23	1.09359476080622E-5	2.132509783572129E-4	0.0	1.202954236886842E-4	2.7339869020155502E-5
24-25	1.09359476080622E-5	2.351228735733373E-4	0.0	1.913790831410885E-4	3.28078428241866E-5
26-27	1.09359476080622E-5	2.515267949854306E-4	0.0	3.335464020458971E-4	3.28078428241866E-5
28-29	1.09359476080622E-5	2.569947687894617E-4	0.0	9.29555546685287E-4	3.28078428241866E-5
30-31	1.09359476080622E-5	2.952705854176794E-4	0.0	0.0031550208849259447	3.28078428241866E-5
32-33	1.09359476080622E-5	2.952705854176794E-4	0.0	0.006933390783511434	3.28078428241866E-5
34-35	1.09359476080622E-5	3.9369411389023913E-4	0.0	0.011799887469099112	3.28078428241866E-5
36-37	1.09359476080622E-5	4.7571372095070566E-4	0.0	0.019088696549872566	3.28078428241866E-5
38-39	1.09359476080622E-5	5.030535899708612E-4	0.0	0.0315611447968675	3.28078428241866E-5
40-41	1.09359476080622E-5	5.358614327950477E-4	0.0	0.042836106780779634	3.28078428241866E-5
42-43	2.18718952161244E-5	5.522653542071411E-4	0.0	0.05218634198567282	3.28078428241866E-5
44-45	3.28078428241866E-5	6.069450922474521E-4	0.0	0.06248800463246741	3.28078428241866E-5
46-47	3.28078428241866E-5	6.452209088756698E-4	0.0	0.0739488777257166	3.82758166282177E-5
48-49	3.28078428241866E-5	6.452209088756698E-4	0.0	0.08476452991009012	4.37437904322488E-5
50-51	3.28078428241866E-5	6.944326731119497E-4	0.0	0.09502791674025649	4.37437904322488E-5
52-53	3.28078428241866E-5	6.999006469159808E-4	0.0	0.10467342253056734	6.56156856483732E-5
54-55	3.82758166282177E-5	7.053686207200119E-4	0.0	0.11401272178785246	8.74875808644976E-5
56-57	4.37437904322488E-5	7.655163325643539E-4	0.0	0.12388788247793261	8.74875808644976E-5
58-59	4.37437904322488E-5	7.655163325643539E-4	0.0	0.13248353729786952	8.74875808644976E-5
60-61	4.37437904322488E-5	7.873882277804784E-4	0.0	0.14171894505287802	9.84235284725598E-5
62-63	4.37437904322488E-5	8.037921491925717E-4	0.0	0.15034193974183507	1.09359476080622E-4
64-65	4.37437904322488E-5	8.858117562530382E-4	0.0	0.1590141461950284	1.09359476080622E-4
66-67	4.37437904322488E-5	9.186195990772248E-4	0.0	0.16797068728603134	1.09359476080622E-4
68-69	4.37437904322488E-5	9.35023520489318E-4	0.0	0.176522598315536	1.09359476080622E-4
70-71	4.37437904322488E-5	9.404914942933491E-4	0.0	0.18481751457625117	1.09359476080622E-4
72-73	4.37437904322488E-5	9.404914942933491E-4	0.0	0.1908213498130773	1.2029542368868419E-4
74-75	4.37437904322488E-5	9.514274419014113E-4	0.0	0.19567144257725289	1.2029542368868419E-4
76-77	4.37437904322488E-5	9.842352847255979E-4	0.0	0.19991459024918104	1.2029542368868419E-4
78-79	4.37437904322488E-5	0.0010061071799417223	0.0	0.20355079282886174	1.2029542368868419E-4
80-81	4.37437904322488E-5	0.0010225111013538158	0.0	0.20524586470811135	1.366993451007775E-4
82-83	4.37437904322488E-5	0.0010279790751578468	0.0	0.20594029738122333	1.531032665128708E-4
84-85	4.37437904322488E-5	0.0010279790751578468	0.0	0.20659645423770703	1.531032665128708E-4
86-87	4.37437904322488E-5	0.001049850970373971	0.0	0.20684251305888846	1.531032665128708E-4
88-89	4.92117642362799E-5	0.0010826588131981577	0.0	0.20695187253496905	1.531032665128708E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9285	0.0	41.989426	1
GTATCAA	15560	0.0	32.565018	1
CTTATAC	8105	0.0	28.381054	1
TCAACGC	18180	0.0	27.023203	4
ATCAACG	18110	0.0	27.015652	3
CAACGCA	18490	0.0	26.41641	5
AACGCAG	19185	0.0	25.56587	6
TATCAAC	19810	0.0	25.209951	2
TATACAC	11905	0.0	23.001753	3
TACATGG	24375	0.0	22.202898	2
GTACATG	25300	0.0	22.000473	1
ACGCAGA	22720	0.0	21.55237	7
ACATGGG	24630	0.0	21.395937	3
CGCAGAG	22875	0.0	21.388597	8
TTATACA	12035	0.0	20.25941	2
CATGGGG	17820	0.0	19.487345	4
ACATCTC	12615	0.0	19.327839	8
ACACATC	13040	0.0	18.262348	6
TACACAT	15500	0.0	17.823772	5
GCAGAGT	28225	0.0	17.463787	9
>>END_MODULE