FastQCFastQC Report
Fri 27 May 2016
SRR2139888_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139888_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10354770
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA127250.048.3231771
GTATCAA232250.035.266661
ATCAACG275900.028.9466973
TCAACGC278050.028.6790454
CAACGCA283700.028.1222525
TATCAAC293000.027.4260522
AACGCAG292300.027.3645446
ACGCAGA337700.023.6856887
CGCAGAG342250.023.346998
GTACATG374700.022.6967031
TACATGG367850.022.5765442
ACATGGG372350.021.7988033
CTTATAC88800.020.3289411
GCAGAGT408350.019.5877489
CATGGGG255700.019.2182274
GAGTACT229950.017.81770312-13
CAGAGTA403700.017.36079410-11
AGAGTAC375200.017.3002710-11
TATACAC134100.016.6512343
ATGGGGG160300.016.3191785
Produced by FastQC (version 0.11.5)