##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139888_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10354770
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.359226134428866	33.0	31.0	34.0	31.0	34.0
2	32.52934821343207	34.0	31.0	34.0	31.0	34.0
3	32.65234650310919	34.0	31.0	34.0	31.0	34.0
4	36.1701951854073	37.0	35.0	37.0	35.0	37.0
5	36.00535907605867	37.0	35.0	37.0	35.0	37.0
6	36.049355997284344	37.0	35.0	37.0	35.0	37.0
7	36.030604059771484	37.0	35.0	37.0	35.0	37.0
8	36.02278891757132	37.0	35.0	37.0	35.0	37.0
9	37.87466645806715	39.0	38.0	39.0	35.0	39.0
10-11	37.75445244075919	39.0	37.5	39.0	35.0	39.0
12-13	37.77220237629614	39.0	37.5	39.0	35.0	39.0
14-15	39.09126431586602	40.0	38.5	41.0	36.0	41.0
16-17	39.04655955661015	40.0	38.0	41.0	36.0	41.0
18-19	39.12151694339903	40.0	38.5	41.0	36.0	41.0
20-21	39.125970225154646	40.0	39.0	41.0	36.0	41.0
22-23	39.08998042641001	40.0	39.0	41.0	36.0	41.0
24-25	39.07234522379105	40.0	39.0	41.0	36.0	41.0
26-27	38.97633113066652	40.0	38.5	41.0	35.5	41.0
28-29	38.89769380442425	40.0	38.0	41.0	35.0	41.0
30-31	38.752874549932045	40.0	38.0	41.0	35.0	41.0
32-33	38.65960076305082	40.0	38.0	41.0	35.0	41.0
34-35	38.61457720113128	40.0	38.0	41.0	35.0	41.0
36-37	38.518780325083156	40.0	38.0	41.0	34.5	41.0
38-39	38.486120196290884	40.0	38.0	41.0	34.0	41.0
40-41	38.499975776808796	40.0	38.0	41.0	34.0	41.0
42-43	38.46962243256998	40.0	38.0	41.0	34.0	41.0
44-45	38.36917756593782	40.0	38.0	41.0	34.0	41.0
46-47	38.319688005652864	40.0	38.0	41.0	34.0	41.0
48-49	38.303753107071955	40.0	38.0	41.0	34.0	41.0
50-51	38.23295265955777	40.0	38.0	41.0	34.0	41.0
52-53	38.11302523162637	40.0	37.0	41.0	33.5	41.0
54-55	37.94931291478014	40.0	37.0	41.0	33.0	41.0
56-57	37.83155116112539	40.0	37.0	41.0	33.0	41.0
58-59	37.61948872646727	39.0	36.0	41.0	33.0	41.0
60-61	37.466873872278974	39.0	36.0	41.0	32.5	41.0
62-63	37.272553476904335	39.0	35.5	40.0	32.0	41.0
64-65	36.96894581880663	39.0	35.0	40.0	32.0	41.0
66-67	36.910487950843255	38.0	35.0	40.0	32.0	41.0
68-69	36.686959756729834	38.0	35.0	40.0	32.0	41.0
70-71	36.382205219267384	37.0	35.0	40.0	31.0	41.0
72-73	36.07530767491539	37.0	35.0	39.0	31.0	41.0
74-75	35.68195700286945	36.0	34.0	39.0	31.0	40.5
76-77	33.631957371387635	34.5	32.0	36.5	28.0	39.0
78-79	34.80685354720542	35.0	34.0	37.0	30.0	39.0
80-81	34.804748686488644	35.0	34.0	37.0	31.0	39.0
82-83	34.513319067113635	35.0	34.0	36.5	30.5	39.0
84-85	34.25020785009595	35.0	34.0	36.0	30.0	37.5
86-87	34.03350032185496	35.0	34.0	36.0	30.0	37.0
88-89	33.78781775012409	35.0	34.0	35.5	30.0	37.0
90-91	33.614370255946504	35.0	34.0	35.0	30.0	36.0
92-93	33.54317997332895	35.0	33.5	35.0	30.0	36.0
94-95	33.51152958073934	35.0	33.5	35.0	30.0	36.0
96-97	33.53396214703356	35.0	34.0	35.0	30.0	36.0
98-99	33.55288670649546	35.0	33.0	35.0	30.0	35.0
100-101	32.311171678579214	34.0	31.0	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	6.0
19	12.0
20	60.0
21	214.0
22	828.0
23	2770.0
24	7094.0
25	15322.0
26	28545.0
27	47444.0
28	71073.0
29	100202.0
30	135655.0
31	179076.0
32	236828.0
33	323580.0
34	470651.0
35	716140.0
36	1265188.0
37	2381532.0
38	2989204.0
39	1362926.0
40	20417.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.7812563678382	20.782866253910033	13.536331565066147	23.899545813185615
2	14.421113771108768	22.049041222580765	41.480118073505814	22.049726932804646
3	17.87007204613456	27.42474616007295	31.0031025362843	23.702079257508192
4	11.882998065915329	18.519910970490987	39.99399696893286	29.603093994660817
5	12.3026330212031	38.8231440248875	36.272877041368126	12.601345912541277
6	28.45661468096346	39.15986545331282	19.52930871472761	12.854211150996111
7	25.289501926986862	33.24552836389269	24.292569789354022	17.172399919766423
8	23.961887967195683	37.015452351025885	22.161146567246774	16.86151311453166
9	25.611547545065044	16.313162407029427	21.72037870524218	36.35491134266335
10-11	23.58665879589223	27.113480391269235	29.892182461934784	19.40767835090375
12-13	24.506009223981113	24.854911968886157	29.817495317124088	20.821583490008642
14-15	21.621985078662068	26.151424824224705	27.373398062931102	24.853192034182126
16-17	21.05325829570112	29.33204683412113	28.055565601971395	21.559129268206355
18-19	21.078730917187485	28.492652588741834	29.736301711013464	20.69231478305722
20-21	22.033434567821708	27.70139410576827	29.45578345937625	20.80938786703377
22-23	21.65970992988917	27.832565235736478	29.286740622772623	21.22098421160173
24-25	21.405433526932992	27.949301637960826	29.437259967862474	21.20800486724371
26-27	21.38174322506079	28.095694617868812	29.314411903970967	21.208150253099433
28-29	21.20744968798405	28.201457402963946	29.338002641310702	21.2530902677413
30-31	21.611319793231914	27.988118294190915	29.248064366364517	21.15249754621265
32-33	21.205007303261773	28.259159917913156	29.211310480052955	21.324522298772116
34-35	21.392952256821633	28.296723314760147	29.277825385666663	21.03249904275156
36-37	21.355412908201448	28.241662106031473	29.171392114460037	21.231532871307042
38-39	21.202882136417152	28.18117918225216	29.292689348215927	21.323249333114763
40-41	21.409295402003327	28.06466964721927	29.239097544139327	21.286937406638078
42-43	21.426951367192963	28.113866541438952	29.04491353182494	21.414268559543142
44-45	21.43284774626385	28.34507885471094	28.78443617694585	21.43763722207936
46-47	21.634920856687494	28.2194386410209	28.46166887114509	21.683971631146516
48-49	21.509020219003745	28.36290644246982	28.45333727353635	21.67473606499009
50-51	21.424833129523975	28.480656395417732	28.44684411187366	21.647666363184637
52-53	21.560770192516685	28.40627470379036	28.430801953787388	21.602153149905575
54-55	21.569215363783247	28.34847802738951	28.33778294234356	21.74452366648368
56-57	21.653296476690027	28.504842077472237	28.166206097638486	21.67565534819925
58-59	21.503618716441917	28.606754418618603	28.323223736804138	21.566403128135345
60-61	21.65109656937436	28.672558203214603	28.125284338496908	21.55106088891413
62-63	21.537229519209816	28.701350103448668	28.10976587700248	21.651654500339028
64-65	21.528625338460348	28.626025943010276	28.14321333274496	21.70213538578442
66-67	21.650805286133583	28.62088529328506	27.978688597863492	21.749620822717862
68-69	21.640486798134337	28.541568580504013	28.11357163247456	21.704372988887094
70-71	21.635498267671867	28.332139153562924	28.124204519709494	21.908158059055722
72-73	21.67817680762447	28.356329946356322	28.147489340901068	21.818003905118136
74-75	21.57843403857378	28.471083811281733	28.102488118810502	21.847994031333982
76-77	21.666791887171648	28.41105939813837	28.038619669073274	21.883529045616708
78-79	21.68905138174521	28.40765635749907	28.01527622298285	21.888016037772875
80-81	21.690257005585305	28.35727209803065	28.037835608098337	21.91463528828571
82-83	21.736407627662484	28.29225368397727	28.161441205501735	21.809897482858513
84-85	21.884601681967226	28.26200461741304	28.030603337933695	21.822790362686046
86-87	21.68478069829231	28.287288078882277	28.148077400253353	21.87985382257206
88-89	21.845323088400793	28.221705748983478	28.063343497086855	21.869627665528874
90-91	21.844872877789513	28.1701243692618	28.17536876151319	21.809633991435494
92-93	21.83465143675943	28.088381128234403	28.200013397037218	21.87695403796895
94-95	21.72357374814131	28.084020435116425	28.26914920106443	21.923256615677833
96-97	21.72569827826783	28.127267432689855	28.325641251198174	21.821393037844132
98-99	21.974913639976062	28.71871329105307	28.338072184631557	20.96830088433931
100-101	25.79451255574805	35.621618889653334	29.143275885276935	9.440592669321681
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1978.0
1	1874.5
2	2944.0
3	4420.0
4	6175.0
5	10422.0
6	13926.0
7	16118.5
8	17561.5
9	17159.0
10	15570.5
11	14046.5
12	15287.5
13	20268.5
14	31000.5
15	45149.0
16	56414.0
17	63148.5
18	65420.5
19	63686.5
20	60338.5
21	57919.0
22	59114.0
23	66435.0
24	80536.0
25	100053.5
26	125088.5
27	152818.0
28	180589.5
29	207357.0
30	230234.0
31	251368.5
32	273253.0
33	295412.0
34	317786.5
35	339871.0
36	361880.0
37	379517.5
38	392058.0
39	403153.5
40	413429.0
41	420173.0
42	423896.5
43	429980.5
44	436752.0
45	440276.5
46	443144.0
47	445904.5
48	441526.5
49	431576.5
50	420427.0
51	404371.0
52	381213.5
53	357023.5
54	333748.5
55	307808.5
56	279941.0
57	250158.5
58	219994.0
59	190650.0
60	160812.0
61	131224.5
62	104359.0
63	81790.5
64	62157.5
65	46615.5
66	35067.0
67	26430.5
68	19895.0
69	14109.0
70	9910.5
71	7226.0
72	5094.0
73	3387.0
74	2273.0
75	1558.0
76	1111.0
77	756.0
78	511.0
79	351.5
80	236.0
81	162.5
82	119.5
83	88.5
84	61.0
85	44.5
86	37.5
87	35.0
88	28.5
89	19.0
90	13.0
91	9.0
92	5.5
93	3.0
94	1.0
95	0.0
96	0.0
97	1.0
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.005234302645061165
3	1.5451815926379822E-4
4	9.657384953987389E-6
5	1.0623123449386128E-4
6	0.0
7	2.8972154861962168E-5
8	3.380084733895586E-4
9	1.4486077430981084E-4
10-11	7.243038715490542E-5
12-13	2.1246246898772256E-4
14-15	1.4486077430981084E-5
16-17	5.263274799923127E-4
18-19	4.8286924769936944E-6
20-21	5.794666289755817E-5
22-23	0.0
24-25	0.0
26-27	7.248942657142458E-5
28-29	0.0
30-31	8.724138805119654E-5
32-33	4.523930277770292E-4
34-35	0.0
36-37	3.3946360322903684E-4
38-39	0.0010878679918389037
40-41	0.0
42-43	0.0
44-45	2.723621532746282E-4
46-47	0.0
48-49	0.0
50-51	3.2140943608821574E-5
52-53	6.072138194491996E-4
54-55	0.003177145971420063
56-57	0.0034640871468688433
58-59	0.004155382903875167
60-61	9.378971944578281E-4
62-63	5.798367005900128E-6
64-65	4.5831967432979117E-4
66-67	0.0
68-69	0.0
70-71	6.13974137321824E-6
72-73	1.7440206252862062E-4
74-75	2.5260980664108653E-5
76-77	0.0
78-79	0.0
80-81	4.5532706935264765E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0011217443424609741
92-93	1.529007344404754E-4
94-95	2.2265321287101817E-5
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	278.0
20-21	763.0
22-23	1771.0
24-25	4224.0
26-27	7212.0
28-29	15889.0
30-31	35854.0
32-33	44389.0
34-35	59929.0
36-37	91799.0
38-39	107678.0
40-41	105177.0
42-43	116914.0
44-45	136777.0
46-47	135699.0
48-49	127235.0
50-51	113912.0
52-53	108669.0
54-55	117166.0
56-57	124330.0
58-59	124484.0
60-61	123163.0
62-63	114010.0
64-65	116287.0
66-67	125394.0
68-69	122010.0
70-71	118575.0
72-73	110542.0
74-75	111567.0
76-77	109554.0
78-79	116586.0
80-81	118935.0
82-83	111269.0
84-85	108853.0
86-87	113699.0
88-89	121810.0
90-91	133782.0
92-93	127390.0
94-95	137409.0
96-97	199635.0
98-99	790629.0
100-101	5643522.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.64542357513648
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.85328789240913	76.95522901164063
2	7.155799222482518	12.257229108562951
3	1.7060345339794902	4.3834215088945205
4	0.6258722201418373	2.1441236559183485
5	0.2735261251361491	1.171313042307564
6	0.13521524171893168	0.6948339990499421
7	0.08119711863545932	0.4867913133030165
8	0.04557658443724726	0.31227407033888116
9	0.028761180117237602	0.22169371082954473
>10	0.09304085790811555	1.2098247703856246
>50	0.001375698311917377	0.07703257851619866
>100	2.4358412597007872E-4	0.04479707080301911
>500	5.230815326288651E-5	0.025311662907696566
>1k	1.7432442701079078E-5	0.016124496542098715
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.8972154861962168E-5	0.0	0.0	0.0
3	0.0	2.8972154861962168E-5	0.0	0.0	0.0
4	0.0	7.725907963189911E-5	0.0	9.657384953987389E-6	0.0
5	0.0	8.69164645858865E-5	0.0	9.657384953987389E-6	0.0
6	0.0	8.69164645858865E-5	0.0	9.657384953987389E-6	0.0
7	0.0	8.69164645858865E-5	0.0	1.9314769907974778E-5	0.0
8	0.0	1.0623123449386128E-4	0.0	1.9314769907974778E-5	0.0
9	0.0	2.0280508403373518E-4	0.0	4.828692476993695E-5	0.0
10-11	0.0	2.0280508403373518E-4	0.0	8.208777210889281E-5	0.0
12-13	0.0	2.0280508403373518E-4	0.0	9.65738495398739E-5	0.0
14-15	0.0	3.2835108843557126E-4	0.0	9.65738495398739E-5	4.8286924769936944E-6
16-17	0.0	3.2835108843557126E-4	0.0	1.0623123449386128E-4	9.657384953987389E-6
18-19	0.0	3.718093207285145E-4	0.0	1.2554600440183608E-4	9.657384953987389E-6
20-21	0.0	4.1526755302145776E-4	0.0	1.5934685174079193E-4	9.657384953987389E-6
22-23	9.657384953987389E-6	4.58725785314401E-4	0.0	2.3177723889569735E-4	9.657384953987389E-6
24-25	9.657384953987389E-6	5.21498787515319E-4	0.0	2.897215486196217E-4	9.657384953987389E-6
26-27	9.657384953987389E-6	5.552996348542748E-4	0.0	4.442397078834199E-4	9.657384953987389E-6
28-29	9.657384953987389E-6	5.601283273312686E-4	0.0	0.0017141858293327618	9.657384953987389E-6
30-31	9.657384953987389E-6	6.95331716687092E-4	0.0	0.004254078072231445	1.4486077430981084E-5
32-33	9.657384953987389E-6	6.95331716687092E-4	0.0	0.008928252389961342	1.9314769907974778E-5
34-35	9.657384953987389E-6	7.822481812729785E-4	0.0	0.014862715444186592	2.8972154861962168E-5
36-37	9.657384953987389E-6	8.739933383358587E-4	0.0	0.024433183933588093	2.8972154861962168E-5
38-39	1.4486077430981084E-5	9.367663405367767E-4	0.0	0.0389047752871382	2.8972154861962168E-5
40-41	1.9314769907974778E-5	0.0010526549599846255	0.0	0.05259411845941532	2.8972154861962168E-5
42-43	1.9314769907974778E-5	0.0010719697298926002	0.0	0.06475276611648545	2.8972154861962168E-5
44-45	1.9314769907974778E-5	0.0011733722719094678	0.0	0.07811858689280399	2.8972154861962168E-5
46-47	1.9314769907974778E-5	0.0012554600440183607	0.0	0.09277366856047986	2.8972154861962168E-5
48-49	1.9314769907974778E-5	0.0012602887364953543	0.0	0.1069024227481634	2.8972154861962168E-5
50-51	1.9314769907974778E-5	0.0013713486634662094	0.0	0.12078491361952029	2.8972154861962168E-5
52-53	1.9314769907974778E-5	0.0013713486634662094	0.0	0.13351817568135266	3.380084733895586E-5
54-55	1.9314769907974778E-5	0.0013713486634662094	0.0	0.14668602006611445	3.8629539815949555E-5
56-57	1.9314769907974778E-5	0.0013810060484201966	0.0	0.15696147765715704	3.8629539815949555E-5
58-59	1.9314769907974778E-5	0.0013858347408971902	0.0	0.16556137895868284	3.8629539815949555E-5
60-61	1.9314769907974778E-5	0.0014196355882361462	0.0	0.17393433171378989	3.8629539815949555E-5
62-63	1.9314769907974778E-5	0.001438950358144121	0.0	0.18177129960395064	3.8629539815949555E-5
64-65	1.9314769907974778E-5	0.001564496362545957	0.0	0.18932337463796878	3.8629539815949555E-5
66-67	1.9314769907974778E-5	0.001564496362545957	0.0	0.19694305136666485	3.8629539815949555E-5
68-69	1.9314769907974778E-5	0.0016031259023619067	0.0	0.20430197870160322	3.8629539815949555E-5
70-71	1.9314769907974778E-5	0.0016417554421778563	0.0	0.21110077770921035	3.8629539815949555E-5
72-73	1.9314769907974778E-5	0.0016514128271318436	0.0	0.21740222139168713	3.8629539815949555E-5
74-75	1.9314769907974778E-5	0.0016803849819938058	0.0	0.22238543202794459	3.8629539815949555E-5
76-77	1.9314769907974778E-5	0.0017286719067637429	0.0	0.22686162995411777	3.8629539815949555E-5
78-79	1.9314769907974778E-5	0.0017479866766717176	0.0	0.2304396910795701	3.8629539815949555E-5
80-81	2.8972154861962168E-5	0.0017479866766717176	0.0	0.23202350221202403	3.8629539815949555E-5
82-83	2.8972154861962168E-5	0.0017479866766717176	0.0	0.23266088961898718	3.8629539815949555E-5
84-85	2.8972154861962168E-5	0.0017673014465796923	0.0	0.2331872170989795	3.8629539815949555E-5
86-87	2.8972154861962168E-5	0.0017673014465796923	0.0	0.23344313780026016	3.8629539815949555E-5
88-89	2.8972154861962168E-5	0.0018638752961195662	0.0	0.23353488295732305	3.8629539815949555E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12725	0.0	48.323177	1
GTATCAA	23225	0.0	35.26666	1
ATCAACG	27590	0.0	28.946697	3
TCAACGC	27805	0.0	28.679045	4
CAACGCA	28370	0.0	28.122252	5
TATCAAC	29300	0.0	27.426052	2
AACGCAG	29230	0.0	27.364544	6
ACGCAGA	33770	0.0	23.685688	7
CGCAGAG	34225	0.0	23.34699	8
GTACATG	37470	0.0	22.696703	1
TACATGG	36785	0.0	22.576544	2
ACATGGG	37235	0.0	21.798803	3
CTTATAC	8880	0.0	20.328941	1
GCAGAGT	40835	0.0	19.587748	9
CATGGGG	25570	0.0	19.218227	4
GAGTACT	22995	0.0	17.817703	12-13
CAGAGTA	40370	0.0	17.360794	10-11
AGAGTAC	37520	0.0	17.30027	10-11
TATACAC	13410	0.0	16.651234	3
ATGGGGG	16030	0.0	16.319178	5
>>END_MODULE