FastQCFastQC Report
Fri 27 May 2016
SRR2139887_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139887_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10984615
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA122450.042.793611
GTATCAA204700.031.8359871
TCAACGC230850.027.3826164
ATCAACG232250.027.2712733
CAACGCA236050.026.7264255
AACGCAG242300.026.1061576
TATCAAC254650.025.36292
GTACATG276850.022.3524721
ACGCAGA283800.022.2593547
TACATGG277300.022.016452
CGCAGAG287150.021.956228
ACATGGG278300.021.3391083
CATGGGG212600.019.541754
GCAGAGT354400.017.8131739
GAGTACT207800.017.60141612-13
AGAGTAC306450.017.07866310-11
CAGAGTA338750.016.7085610-11
ATGGGGG142550.016.10395
GTACTTT238250.015.43076614-15
AGTACTT235350.014.78117712-13
Produced by FastQC (version 0.11.5)