##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139887_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10984615
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.37496452993573	33.0	31.0	34.0	31.0	34.0
2	32.52809297367272	34.0	31.0	34.0	31.0	34.0
3	32.64684315290067	34.0	31.0	34.0	31.0	34.0
4	36.16224082500843	37.0	35.0	37.0	35.0	37.0
5	36.00669927894605	37.0	35.0	37.0	35.0	37.0
6	36.05005564601035	37.0	35.0	37.0	35.0	37.0
7	36.02655659756851	37.0	35.0	37.0	35.0	37.0
8	36.01437619798236	37.0	35.0	37.0	35.0	37.0
9	37.85299284499275	39.0	38.0	39.0	35.0	39.0
10-11	37.73114182882149	39.0	37.0	39.0	35.0	39.0
12-13	37.74961894431439	39.0	37.0	39.0	35.0	39.0
14-15	39.079504880234765	40.0	38.5	41.0	36.0	41.0
16-17	39.03163779522541	40.0	38.0	41.0	36.0	41.0
18-19	39.09306602916898	40.0	38.5	41.0	36.0	41.0
20-21	39.09069076327525	40.0	39.0	41.0	36.0	41.0
22-23	39.05589839772786	40.0	39.0	41.0	36.0	41.0
24-25	39.039778119167636	40.0	39.0	41.0	36.0	41.0
26-27	38.94426836067693	40.0	38.0	41.0	35.0	41.0
28-29	38.86608123366262	40.0	38.0	41.0	35.0	41.0
30-31	38.723133958568525	40.0	38.0	41.0	35.0	41.0
32-33	38.636024740198636	40.0	38.0	41.0	35.0	41.0
34-35	38.59078852807318	40.0	38.0	41.0	35.0	41.0
36-37	38.492272482178734	40.0	38.0	41.0	34.0	41.0
38-39	38.45657976181771	40.0	38.0	41.0	34.0	41.0
40-41	38.45713665781958	40.0	38.0	41.0	34.0	41.0
42-43	38.420260435421845	40.0	38.0	41.0	34.0	41.0
44-45	38.31493152226512	40.0	38.0	41.0	34.0	41.0
46-47	38.25961390394785	40.0	38.0	41.0	34.0	41.0
48-49	38.2358453274646	40.0	38.0	41.0	34.0	41.0
50-51	38.159138580059064	40.0	37.5	41.0	33.0	41.0
52-53	38.03036135851106	40.0	37.0	41.0	33.0	41.0
54-55	37.8566981293736	40.0	37.0	41.0	33.0	41.0
56-57	37.73483983293943	39.0	37.0	41.0	33.0	41.0
58-59	37.51576350843615	39.0	36.0	41.0	32.5	41.0
60-61	37.353062506870685	39.0	36.0	40.5	32.0	41.0
62-63	37.14208329775377	39.0	35.0	40.0	32.0	41.0
64-65	36.82457170060886	38.5	35.0	40.0	31.0	41.0
66-67	36.75314779137885	38.0	35.0	40.0	31.5	41.0
68-69	36.52086694801215	37.5	35.0	40.0	31.0	41.0
70-71	36.21038446173863	37.0	35.0	39.5	31.0	41.0
72-73	35.89968974600458	36.5	34.5	39.0	31.0	41.0
74-75	35.498740404367034	36.0	34.0	39.0	30.5	40.0
76-77	33.45414928566747	34.5	32.0	36.5	27.5	39.0
78-79	34.652523604751394	35.0	34.0	37.0	30.0	39.0
80-81	34.660736375661074	35.0	34.0	37.0	30.0	39.0
82-83	34.37583725222753	35.0	34.0	36.5	30.0	39.0
84-85	34.11795051819716	35.0	34.0	36.0	30.0	37.0
86-87	33.91004103670656	35.0	34.0	36.0	30.0	37.0
88-89	33.67369439618145	35.0	33.5	35.0	30.0	37.0
90-91	33.508590910714034	35.0	33.0	35.0	29.5	36.0
92-93	33.4420540267153	35.0	33.0	35.0	30.0	36.0
94-95	33.41463373945423	35.0	33.0	35.0	30.0	36.0
96-97	33.446480591901846	35.0	33.0	35.0	30.0	35.5
98-99	33.47188971973422	35.0	33.0	35.0	30.0	35.0
100-101	32.22297750954817	34.0	31.0	34.5	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	4.0
19	15.0
20	50.0
21	217.0
22	926.0
23	3168.0
24	8198.0
25	17886.0
26	33271.0
27	55095.0
28	81925.0
29	114183.0
30	153687.0
31	201323.0
32	264473.0
33	358557.0
34	520730.0
35	794486.0
36	1405203.0
37	2579929.0
38	3069151.0
39	1301427.0
40	20711.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.10280469547635	20.270869757383394	13.326083799932906	24.300241747207345
2	14.84180294146539	21.76302382795085	40.6207799862292	22.774393244354556
3	18.0796330574898	26.449389322824324	30.72759349012787	24.743384129558002
4	12.276127732140198	18.08694744975972	39.404705418816796	30.232219399283288
5	12.778798043699144	38.1624962709187	36.18477453316727	12.873931152214885
6	29.58320341677883	38.587715636824775	18.990005566876945	12.839075379519446
7	26.05512583327917	32.7087415179086	23.873619956058537	17.3625126927537
8	24.326821439570626	36.81235682753813	21.734165761836586	17.126655971054664
9	25.571430015009206	16.268552921087366	21.33856417594428	36.82145288795915
10-11	23.889260494742302	26.800684503292448	29.683937273134248	19.626117728831
12-13	24.70840390760271	24.393043834649582	29.608820504986692	21.289731752761014
14-15	21.798928109760073	25.819242525010093	26.99520561192253	25.386623753307298
16-17	21.576290433792444	28.924034476750187	27.639285936025487	21.860389153431882
18-19	21.52694017951471	28.280394897772933	29.181236666009685	21.01142825670267
20-21	22.25909361113057	27.52499254264779	29.005000989382268	21.210912856839368
22-23	21.976491450294215	27.4964927832551	28.822383690968678	21.704632075482007
24-25	21.748542042259192	27.59627402501979	28.949604696647523	21.705579236073493
26-27	21.758441427575605	27.751240702419196	28.84053783952188	21.649780030483317
28-29	21.669964429749896	27.828112989569647	28.862335563958517	21.63958701672194
30-31	21.947505657418727	27.76272288987598	28.705426591658224	21.58434486104707
32-33	21.78835025384547	27.852525198028594	28.667520947615245	21.6916036005107
34-35	21.878744827486663	27.87317173583202	28.693285651447198	21.554797785234125
36-37	21.845969518100876	27.820826207589946	28.653529573294634	21.679674701014545
38-39	21.681589894244258	27.939788898348183	28.723935969061277	21.654685238346282
40-41	21.909981464882158	27.81076104090907	28.612363315366917	21.666894178841854
42-43	21.79038381819382	27.944118199456252	28.432816662438498	21.832681319911433
44-45	21.90721123709252	28.017409026892192	28.272472293481503	21.802907442533787
46-47	21.96319593999334	27.98404136149715	28.0356094785892	22.017153219920306
48-49	21.892361838049318	28.074067304298865	28.062795301707727	21.97077555594409
50-51	21.88259248696653	28.15746453920837	28.022944712182547	21.93699826164255
52-53	22.05461679913119	28.037086326748405	27.907169391449845	22.00112748267056
54-55	21.966483989994153	27.9601988841222	27.955112864345633	22.118204261538015
56-57	22.008985270960444	28.115513886830335	27.866720289094353	22.008780553114864
58-59	22.012601155710136	28.19532412811624	27.80243654107287	21.989638175100755
60-61	22.068232640444847	28.13861829351163	27.83402915999964	21.959119906043878
62-63	22.02470108586029	28.191082615490043	27.819465290262222	21.964751008387445
64-65	22.01044579448827	28.14057234693487	27.788624674229002	22.060357184347854
66-67	22.059217620231923	28.206053935248903	27.670812055261646	22.06391638925753
68-69	22.040147867785116	28.173808672627505	27.709931614034634	22.07611184555274
70-71	22.011572630391147	27.932182276751693	27.800098319640615	22.256146773216546
72-73	22.0936398240806	27.98315092710056	27.765095873952177	22.158113374866662
74-75	21.938457662562453	28.08199622648017	27.7652236149114	22.214322496045984
76-77	22.072820186352573	28.01489909160118	27.687958562819563	22.224322159226684
78-79	22.046332455798513	28.02870359742403	27.67339486330712	22.251569083470336
80-81	22.067984276133622	28.016314372771124	27.671747358315596	22.243953992779662
82-83	22.08279177535234	27.978905313662167	27.78782178576363	22.150481125221862
84-85	22.202657012487755	27.941709193512892	27.699817237324385	22.155816556674967
86-87	22.0193794145848	27.932784208428256	27.81627264177251	22.23156373521443
88-89	22.160988907418812	27.910145585767648	27.718385722491696	22.21047978432185
90-91	22.195846216904616	27.88591890254914	27.75761252752823	22.160622353018013
92-93	22.180176426710357	27.78786701989384	27.85231344048995	22.17964311290585
94-95	22.087036474986405	27.76681576693815	27.91711911865124	22.229028639424207
96-97	22.087014195805878	27.823546898418883	27.921053293577902	22.16838561219734
98-99	22.325135439575174	28.410141241103616	27.96940152505385	21.295321794267355
100-101	26.339165363608853	35.32280228411121	28.711627978225124	9.626404374054811
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1534.0
1	1461.0
2	2470.5
3	3834.5
4	5282.5
5	8721.0
6	11699.5
7	13684.5
8	15125.5
9	15023.5
10	13678.0
11	12171.0
12	12892.5
13	16811.0
14	25802.5
15	38198.5
16	48232.5
17	53959.5
18	55795.5
19	54519.5
20	52066.5
21	50418.0
22	51779.5
23	58957.0
24	71710.0
25	90058.5
26	113441.5
27	138650.0
28	164626.5
29	191214.0
30	214900.0
31	236932.0
32	261487.5
33	284838.0
34	306426.5
35	327221.0
36	350665.5
37	372657.5
38	390056.0
39	405075.5
40	419832.0
41	434645.0
42	444416.5
43	451357.0
44	459296.0
45	467099.5
46	471071.0
47	471773.5
48	468225.5
49	458935.5
50	447557.5
51	432791.5
52	412267.5
53	390385.0
54	367299.5
55	340443.0
56	312475.0
57	283342.5
58	251961.0
59	219157.5
60	185294.5
61	152132.5
62	121579.5
63	95264.5
64	72715.5
65	55205.0
66	42392.0
67	33027.0
68	25595.5
69	18587.5
70	13276.5
71	9719.5
72	6959.0
73	4807.0
74	3257.0
75	2251.0
76	1593.0
77	1066.0
78	719.5
79	504.5
80	355.0
81	243.5
82	164.0
83	119.5
84	97.5
85	74.0
86	60.0
87	51.5
88	35.0
89	20.0
90	13.0
91	9.5
92	7.5
93	4.0
94	2.5
95	2.5
96	1.5
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.004533613604118124
3	5.462185065202558E-5
4	2.731092532601279E-5
5	5.462185065202558E-5
6	0.0
7	9.103641775337598E-6
8	4.3697480521620466E-4
9	2.1848740260810233E-4
10-11	6.827731331503197E-5
12-13	2.594537905971215E-4
14-15	1.3655462663006396E-5
16-17	4.8249301409289267E-4
18-19	0.0
20-21	4.55202124992768E-5
22-23	0.0
24-25	0.0
26-27	1.1844183030164765E-4
28-29	4.559119262046367E-6
30-31	8.67701505749824E-5
32-33	4.625718039677486E-4
34-35	0.0
36-37	3.4197905683285246E-4
38-39	0.0011782582204584468
40-41	0.0
42-43	0.0
44-45	2.9211298298068353E-4
46-47	0.0
48-49	4.90300219159295E-6
50-51	8.427311071087443E-5
52-53	5.55925198511623E-4
54-55	0.003294417707874721
56-57	0.003592669112615847
58-59	0.004443248070268363
60-61	0.0010009280856752517
62-63	0.0
64-65	6.057951543893076E-4
66-67	0.0
68-69	0.0
70-71	1.6703979645198563E-5
72-73	2.4250839403327837E-4
74-75	3.997129147642474E-5
76-77	0.0
78-79	0.0
80-81	5.227795857898528E-4
82-83	0.0
84-85	0.0
86-87	2.4771242222681454E-5
88-89	0.0
90-91	0.001348355418564349
92-93	1.8861062390313166E-4
94-95	6.622459541739045E-6
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	307.0
20-21	886.0
22-23	1788.0
24-25	4408.0
26-27	7111.0
28-29	14762.0
30-31	29677.0
32-33	37239.0
34-35	50602.0
36-37	78327.0
38-39	92679.0
40-41	92213.0
42-43	103802.0
44-45	122305.0
46-47	121585.0
48-49	113977.0
50-51	104455.0
52-53	101296.0
54-55	108919.0
56-57	114646.0
58-59	114205.0
60-61	111070.0
62-63	105378.0
64-65	108657.0
66-67	117169.0
68-69	117901.0
70-71	116132.0
72-73	109542.0
74-75	110934.0
76-77	110085.0
78-79	116181.0
80-81	119477.0
82-83	112862.0
84-85	111730.0
86-87	116532.0
88-89	125515.0
90-91	139726.0
92-93	133989.0
94-95	148125.0
96-97	220774.0
98-99	894877.0
100-101	6322770.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.18103565753626
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.3328022696407	75.20127813248259
2	7.270089497863068	12.240073265061815
3	1.8686298888100188	4.719095979019626
4	0.6943415116901208	2.3380155021637474
5	0.31403781769060707	1.3218014364413933
6	0.17443472401063767	0.8810457433111192
7	0.10037661800300392	0.5914865361505638
8	0.0673441157007583	0.4535277924104624
9	0.04234553614267897	0.32082219791679134
>10	0.13294874213359434	1.6952694195415965
>50	0.002214982019568658	0.12436143600238027
>100	3.44050372571689E-4	0.051217694664126574
>500	5.415094257021525E-5	0.026165606585262482
>1k	3.6094980034483224E-5	0.03583925824854105
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.103641775337598E-6
2	0.0	9.103641775337598E-6	0.0	1.8207283550675195E-5	9.103641775337598E-6
3	0.0	9.103641775337598E-6	0.0	1.8207283550675195E-5	9.103641775337598E-6
4	9.103641775337598E-6	2.731092532601279E-5	0.0	2.731092532601279E-5	9.103641775337598E-6
5	2.731092532601279E-5	3.641456710135039E-5	0.0	2.731092532601279E-5	9.103641775337598E-6
6	5.462185065202558E-5	3.641456710135039E-5	0.0	2.731092532601279E-5	1.8207283550675195E-5
7	7.282913420270078E-5	3.641456710135039E-5	0.0	2.731092532601279E-5	1.8207283550675195E-5
8	7.282913420270078E-5	3.641456710135039E-5	0.0	2.731092532601279E-5	1.8207283550675195E-5
9	1.0924370130405117E-4	6.372549242736319E-5	0.0	4.551820887668799E-5	1.8207283550675195E-5
10-11	1.4110644751773278E-4	6.372549242736319E-5	0.0	7.282913420270078E-5	1.8207283550675195E-5
12-13	1.7296919373141435E-4	6.372549242736319E-5	0.0	8.193277597803838E-5	2.731092532601279E-5
14-15	1.9117647728208954E-4	1.0014005952871357E-4	0.0	9.103641775337597E-5	6.827731331503198E-5
16-17	2.2303922349577112E-4	1.2289916396705756E-4	0.0	1.0924370130405117E-4	7.282913420270078E-5
18-19	2.4124650704644633E-4	1.4110644751773278E-4	0.0	2.0028011905742714E-4	9.103641775337597E-5
20-21	2.640056114847903E-4	1.5476191018073916E-4	0.0	3.004201785861407E-4	9.103641775337597E-5
22-23	2.685574323724591E-4	1.5476191018073916E-4	0.0	4.870448349805614E-4	1.0469188041638237E-4
24-25	2.867647159231343E-4	1.8207283550675195E-4	0.0	7.556022673530205E-4	1.1834734307938876E-4
26-27	2.913165368108031E-4	2.0028011905742714E-4	0.0	0.0013746499080759772	1.1834734307938876E-4
28-29	3.004201785861407E-4	2.0483193994509592E-4	0.0	0.003946428709608848	1.1834734307938876E-4
30-31	3.186274621368159E-4	2.3214286527110874E-4	0.0	0.008612045119469367	1.1834734307938876E-4
32-33	3.186274621368159E-4	2.5035014882178393E-4	0.0	0.015976891315717483	1.2745098485472637E-4
34-35	3.459383874628287E-4	3.231792830244847E-4	0.0	0.02580882443308209	1.4565826840540156E-4
36-37	3.5504202923816627E-4	4.005602381148543E-4	0.0	0.04352451132788905	1.5476191018073916E-4
38-39	3.641456710135039E-4	4.415266261038735E-4	0.0	0.06588760734900587	1.7752101461908316E-4
40-41	3.914565963395167E-4	5.098039394189054E-4	0.0	0.08103151544227996	1.8207283550675195E-4
42-43	4.0966387989019185E-4	5.462185065202559E-4	0.0	0.0927387987653641	1.9572829816975835E-4
44-45	4.0966387989019185E-4	5.780812527339373E-4	0.0	0.10548844907172442	2.0938376083276473E-4
46-47	4.0966387989019185E-4	6.008403571722814E-4	0.0	0.12050945800103144	2.2303922349577112E-4
48-49	4.1876752166552947E-4	6.053921780599502E-4	0.0	0.1320847385183732	2.3669468615877752E-4
50-51	4.3697480521620466E-4	6.372549242736318E-4	0.0	0.14250840835113476	2.3669468615877752E-4
52-53	4.5518208876687985E-4	6.372549242736318E-4	0.0	0.15272269442306352	2.457983279341151E-4
54-55	4.5518208876687985E-4	6.418067451613006E-4	0.0	0.16260924939108018	2.5490196970945274E-4
56-57	4.642857305422175E-4	6.55462207824307E-4	0.0	0.1722500060311627	2.594537905971215E-4
58-59	4.8249301409289267E-4	6.645658495996445E-4	0.0	0.1811032976576785	2.640056114847903E-4
60-61	4.915966558682302E-4	6.873249540379886E-4	0.0	0.18976996462779988	2.822128950354655E-4
62-63	5.007002976435679E-4	7.100840584763325E-4	0.0	0.1980542786433571	2.822128950354655E-4
64-65	5.007002976435679E-4	7.373949838023454E-4	0.0	0.20600175791322684	2.822128950354655E-4
66-67	5.007002976435679E-4	7.464986255776829E-4	0.0	0.21428607192878402	3.004201785861407E-4
68-69	5.007002976435679E-4	7.556022673530205E-4	0.0	0.22289356522736573	3.186274621368159E-4
70-71	5.143557603065742E-4	7.556022673530205E-4	0.0	0.2301673750058605	3.277311039121535E-4
72-73	5.507703274079247E-4	7.601540882406894E-4	0.0	0.23573880377236708	3.823529545641791E-4
74-75	5.553221482955934E-4	7.783613717913646E-4	0.0	0.240522767525307	3.823529545641791E-4
76-77	5.64425790070931E-4	8.056722971173774E-4	0.0	0.24447374805580352	3.823529545641791E-4
78-79	5.735294318462686E-4	8.102241180050462E-4	0.0	0.24791492464688114	3.823529545641791E-4
80-81	5.735294318462686E-4	8.193277597803837E-4	0.0	0.24949440649490218	3.914565963395167E-4
82-83	5.826330736216061E-4	8.193277597803837E-4	0.0	0.2500588322849731	4.460784469915423E-4
84-85	5.962885362846126E-4	8.238795806680525E-4	0.0	0.25061415443326873	4.460784469915423E-4
86-87	6.008403571722814E-4	8.284314015557213E-4	0.0	0.2509691964625069	4.460784469915423E-4
88-89	6.190476407229566E-4	8.648459686570717E-4	0.0	0.2511148547309123	4.460784469915423E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12245	0.0	42.79361	1
GTATCAA	20470	0.0	31.835987	1
TCAACGC	23085	0.0	27.382616	4
ATCAACG	23225	0.0	27.271273	3
CAACGCA	23605	0.0	26.726425	5
AACGCAG	24230	0.0	26.106157	6
TATCAAC	25465	0.0	25.3629	2
GTACATG	27685	0.0	22.352472	1
ACGCAGA	28380	0.0	22.259354	7
TACATGG	27730	0.0	22.01645	2
CGCAGAG	28715	0.0	21.95622	8
ACATGGG	27830	0.0	21.339108	3
CATGGGG	21260	0.0	19.54175	4
GCAGAGT	35440	0.0	17.813173	9
GAGTACT	20780	0.0	17.601416	12-13
AGAGTAC	30645	0.0	17.078663	10-11
CAGAGTA	33875	0.0	16.70856	10-11
ATGGGGG	14255	0.0	16.1039	5
GTACTTT	23825	0.0	15.430766	14-15
AGTACTT	23535	0.0	14.781177	12-13
>>END_MODULE