FastQCFastQC Report
Fri 27 May 2016
SRR2139886_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139886_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17274472
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA178500.046.680381
GTATCAA319950.034.0651931
TCAACGC377000.028.192514
ATCAACG380550.027.8967193
CAACGCA387650.027.3535845
AACGCAG398550.026.7621256
TATCAAC408300.026.368042
ACGCAGA464500.022.8549547
CGCAGAG471000.022.5131178
GTACATG439350.021.3325251
TACATGG438250.020.9494882
ACATGGG436150.020.573043
CATGGGG327150.018.5012154
GCAGAGT571700.018.4602559
AGAGTAC511650.017.525210-11
GAGTACT354800.017.49348312-13
CAGAGTA558950.016.77524610-11
AGTACTT389650.015.80610312-13
GTGGTAT104750.015.3293941
GTACTTT409000.015.24693814-15
Produced by FastQC (version 0.11.5)