##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139886_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17274472
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.02894178183854	33.0	31.0	34.0	30.0	34.0
2	32.21248215285538	33.0	31.0	34.0	30.0	34.0
3	32.32330151682784	34.0	31.0	34.0	30.0	34.0
4	35.93807087128336	37.0	35.0	37.0	35.0	37.0
5	35.71343031497576	37.0	35.0	37.0	33.0	37.0
6	35.74467341172569	37.0	35.0	37.0	33.0	37.0
7	35.71403183842609	37.0	35.0	37.0	33.0	37.0
8	35.68804065328307	37.0	35.0	37.0	33.0	37.0
9	37.48028541769612	39.0	37.0	39.0	35.0	39.0
10-11	37.32078326330321	39.0	37.0	39.0	34.0	39.0
12-13	37.33939228359628	39.0	37.0	39.0	34.0	39.0
14-15	38.55326307513191	40.0	38.0	41.0	34.5	41.0
16-17	38.482402530161266	40.0	38.0	41.0	34.0	41.0
18-19	38.54331596936798	40.0	38.0	41.0	34.0	41.0
20-21	38.54073205119038	40.0	38.0	41.0	34.0	41.0
22-23	38.49770463943146	40.0	38.0	41.0	34.0	41.0
24-25	38.47431409741645	40.0	38.0	41.0	34.0	41.0
26-27	38.35586133661345	40.0	38.0	41.0	34.0	41.0
28-29	38.262444353446256	40.0	38.0	41.0	34.0	41.0
30-31	38.10832401788032	40.0	38.0	41.0	34.0	41.0
32-33	38.0059257389516	40.0	37.0	41.0	33.5	41.0
34-35	37.94633565455338	40.0	37.0	41.0	33.0	41.0
36-37	37.840385265676986	40.0	37.0	41.0	33.0	41.0
38-39	37.79579280307746	40.0	37.0	41.0	33.0	41.0
40-41	37.787451466918284	40.0	37.0	41.0	33.0	41.0
42-43	37.74326653817949	40.0	37.0	41.0	33.0	41.0
44-45	37.62622587571676	39.0	37.0	41.0	33.0	41.0
46-47	37.561974708489394	39.0	37.0	41.0	33.0	41.0
48-49	37.53353794957961	39.0	37.0	40.0	32.0	41.0
50-51	37.438066795546966	39.0	36.0	40.0	32.0	41.0
52-53	37.28486482903563	39.0	36.0	40.0	31.5	41.0
54-55	37.09144365156226	39.0	36.0	40.0	31.0	41.0
56-57	36.95184871366736	39.0	35.0	40.0	31.0	41.0
58-59	36.71756866338163	38.5	35.0	40.0	31.0	41.0
60-61	36.53972806475363	38.0	35.0	40.0	31.0	41.0
62-63	36.320642774733244	38.0	35.0	40.0	30.0	41.0
64-65	36.01034725734468	38.0	34.0	40.0	30.0	41.0
66-67	35.95629938738783	37.0	34.0	40.0	30.0	41.0
68-69	35.73459800553092	37.0	34.0	39.0	30.0	41.0
70-71	35.436973489331706	36.0	34.0	39.0	30.0	40.5
72-73	35.13242808528777	36.0	34.0	39.0	29.0	40.0
74-75	34.73837845867578	35.5	33.5	38.5	29.0	40.0
76-77	32.672150329711215	33.5	31.0	36.0	26.5	38.5
78-79	33.93519294053348	35.0	33.0	37.0	29.0	39.0
80-81	33.998480308344625	35.0	33.0	36.5	29.0	39.0
82-83	33.721691047548575	35.0	33.0	36.0	29.0	38.5
84-85	33.46716875823027	35.0	33.0	36.0	29.0	37.0
86-87	33.264346664006865	35.0	33.0	35.0	29.0	37.0
88-89	33.03816091243098	34.5	32.5	35.0	29.0	36.5
90-91	32.895989486787755	34.0	32.0	35.0	28.5	36.0
92-93	32.84114780987535	34.0	32.0	35.0	29.0	36.0
94-95	32.83385852582178	34.0	32.0	35.0	29.0	35.5
96-97	32.90364315581091	34.0	32.0	35.0	29.0	35.0
98-99	32.97479371593601	34.0	32.0	35.0	29.0	35.0
100-101	31.75831541875626	33.0	30.5	34.5	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	3.0
18	9.0
19	32.0
20	117.0
21	706.0
22	2539.0
23	8087.0
24	21181.0
25	44417.0
26	80022.0
27	128263.0
28	187357.0
29	257743.0
30	340061.0
31	441645.0
32	578612.0
33	782261.0
34	1118161.0
35	1676219.0
36	2726509.0
37	4044389.0
38	3661756.0
39	1164103.0
40	10279.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.497575150198514	20.816265759092374	13.650263811247024	24.03589527946209
2	14.99209475617572	22.108238305515865	40.35949567375249	22.540171264555926
3	18.0441835995606	26.78429269932775	31.0280423172609	24.14348138385075
4	12.425245849956864	18.425844768857885	39.22640355915053	29.92250582203472
5	12.685853834405957	38.34698296247952	36.20002174307946	12.76714146003505
6	29.453299643543374	38.86789709115277	18.85634478437315	12.822458480930704
7	25.792099646715606	32.94412308384837	23.93517415413314	17.328603115302883
8	24.325334410842398	36.861038958783546	21.748343358760156	17.065283271613907
9	25.52696009333549	16.186713354479775	21.32725669308988	36.95906985909485
10-11	23.830447894321246	26.84281606281827	29.702047799973897	19.62468824288659
12-13	24.664144655121497	24.443755436681776	29.59695043054511	21.295149477651616
14-15	21.75329509423966	25.906169729800073	26.972144670388694	25.368390505571575
16-17	21.501651677680023	29.009429586448	27.611983520937954	21.876935214934022
18-19	21.487381581757266	28.27849836906728	29.151677061485703	21.082442987689753
20-21	22.21660770409531	27.53581896609117	29.017574146084762	21.22999918372876
22-23	21.910624494552515	27.54444464001023	28.876119279585062	21.668811585852197
24-25	21.67298216800474	27.659660472972732	29.013682918640427	21.653674440382105
26-27	21.67810672820922	27.807708243393957	28.910375236316714	21.603809792080106
28-29	21.582885974447116	27.85559527902983	28.843359178116252	21.7181595684068
30-31	21.870495890728296	27.68123047947164	28.826296435960476	21.62197719383959
32-33	21.622732176484188	27.888227355063393	28.796771367715913	21.692269100736503
34-35	21.723568691251145	27.977340927052403	28.821988789451876	21.477101592244576
36-37	21.81357119952333	27.841239581063164	28.675508866782167	21.669680352631342
38-39	21.68315819883978	27.937947604233855	28.75792602898148	21.62096816794489
40-41	21.884013084816	27.815368173767475	28.67730583718357	21.623312904232957
42-43	21.837769738720905	27.8940769972987	28.51106683481272	21.757086429167675
44-45	21.851653354246782	28.055554746462057	28.290424475531434	21.802367423759726
46-47	21.990668308861665	27.91934022769238	28.02308057873043	22.066910884715522
48-49	21.896252520915933	28.044887452635912	28.095607510217313	21.96325251623084
50-51	21.840072206297794	28.175613567747725	28.023484292512013	21.960829933442465
52-53	22.022905134434705	28.075274608663875	27.907645405739895	21.994174851161524
54-55	21.93076059268058	28.02985778824261	27.903539327117727	22.135842291959086
56-57	22.018755099083954	28.121293548458482	27.81683533183235	22.043116020625213
58-59	21.93339551344252	28.235461501789434	27.863566484355008	21.967576500413045
60-61	22.016086772356708	28.203480753561934	27.833734114940068	21.94669835914129
62-63	21.95709869546814	28.25661209552257	27.77302790610901	22.013261302900275
64-65	21.876508864992534	28.186390497414592	27.796271355159803	22.14082928243307
66-67	21.96492786189065	28.26663295070514	27.622174960484823	22.146264226919385
68-69	21.94222637110917	28.28314074507864	27.6676845731297	22.10694831068249
70-71	21.97042915004836	28.01378903043878	27.698930468484935	22.316851351027932
72-73	22.01229094514729	27.996394037857254	27.714765184453345	22.276549832542113
74-75	21.886970985522034	28.070473540946416	27.714805040568635	22.327750432962915
76-77	22.05754326128293	28.001848920690716	27.594200000256386	22.34640781776997
78-79	22.022088172470102	28.047146444088188	27.534682189626476	22.39608319381523
80-81	22.05966141548844	28.009728005825107	27.532542466973815	22.398068111712636
82-83	22.066353611408882	27.984242915950798	27.64211310616406	22.307290366476256
84-85	22.22230630197681	27.916662009941795	27.58212289531558	22.278908792765808
86-87	22.010861085588477	27.96071948092474	27.696166193600448	22.332253239886338
88-89	22.11173534673755	27.894917416814824	27.680478941299796	22.312868295147833
90-91	22.124698864021397	27.85600974781176	27.753528965457335	22.265762422709503
92-93	22.10347636430455	27.761011105644435	27.829451861448746	22.30606066860227
94-95	21.983257033975267	27.785791651166402	27.891191338266125	22.339759976592205
96-97	21.916477628281942	27.83635210318905	27.95778705848714	22.28938321004187
98-99	22.131029875335184	28.493811309851413	27.96342483388841	21.41173398092499
100-101	26.232388763866375	35.45908032288271	28.614397985769713	9.694132927481201
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2507.0
1	2384.0
2	4006.5
3	6179.5
4	8558.5
5	14335.5
6	19420.5
7	22876.0
8	25109.0
9	24567.5
10	22039.0
11	19345.5
12	20526.5
13	27305.0
14	43171.0
15	64513.5
16	81369.5
17	91108.0
18	94664.5
19	93314.5
20	90068.5
21	87945.0
22	89427.0
23	99011.5
24	119378.5
25	149778.0
26	187771.5
27	227822.5
28	268617.0
29	310020.0
30	344719.5
31	376656.5
32	412245.5
33	447262.0
34	481099.5
35	513825.0
36	549628.0
37	581283.0
38	604048.5
39	623857.0
40	645262.5
41	665642.5
42	677619.0
43	690468.0
44	705776.5
45	716353.5
46	724918.0
47	732170.5
48	728683.0
49	717879.0
50	705159.5
51	683505.0
52	651271.0
53	616587.5
54	580566.5
55	536295.5
56	490892.5
57	442064.5
58	388136.5
59	336405.0
60	282548.5
61	230244.0
62	184060.5
63	143814.0
64	109354.0
65	81902.5
66	62457.0
67	48308.5
68	36958.5
69	27215.5
70	19880.5
71	14723.5
72	10558.0
73	7247.0
74	5096.0
75	3552.5
76	2407.0
77	1641.5
78	1128.0
79	760.5
80	547.0
81	408.0
82	309.5
83	225.0
84	161.5
85	112.0
86	76.0
87	56.0
88	38.5
89	29.5
90	24.0
91	17.5
92	13.0
93	9.5
94	4.0
95	1.5
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0028655000280182223
3	4.631111156393087E-5
4	7.525555629138767E-5
5	5.788888945491358E-5
6	0.0
7	2.3155555781965434E-5
8	3.589111146204642E-4
9	8.104444523687901E-5
10-11	3.762777814569383E-5
12-13	1.9392777967396053E-4
14-15	2.3155555781965434E-5
16-17	3.849611148751753E-4
18-19	5.7888889454913585E-6
20-21	5.789154366643355E-5
22-23	0.0
24-25	0.0
26-27	7.531655039191402E-5
28-29	5.79702533181231E-6
30-31	4.6447187834691903E-5
32-33	3.9003396846581186E-4
34-35	0.0
36-37	3.199325241760387E-4
38-39	9.754971954012224E-4
40-41	0.0
42-43	0.0
44-45	2.6396605724185874E-4
46-47	0.0
48-49	6.205426614547288E-6
50-51	8.152519594188896E-5
52-53	5.3825455888155E-4
54-55	0.002848053834674343
56-57	0.003107608838104143
58-59	0.003997692834788812
60-61	7.731423817027728E-4
62-63	6.686820297254573E-6
64-65	4.5330230219722725E-4
66-67	0.0
68-69	0.0
70-71	0.0
72-73	9.630156685859332E-5
74-75	1.0841236986585668E-5
76-77	0.0
78-79	0.0
80-81	3.8426047978160744E-4
82-83	0.0
84-85	0.0
86-87	3.94002944226401E-6
88-89	0.0
90-91	8.526733103380804E-4
92-93	1.2899201685051843E-4
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	497.0
20-21	1539.0
22-23	3118.0
24-25	6721.0
26-27	9346.0
28-29	19087.0
30-31	43879.0
32-33	54509.0
34-35	74670.0
36-37	112590.0
38-39	130362.0
40-41	137543.0
42-43	157011.0
44-45	182585.0
46-47	182393.0
48-49	172205.0
50-51	158547.0
52-53	146158.0
54-55	160882.0
56-57	169404.0
58-59	171819.0
60-61	182930.0
62-63	172156.0
64-65	183155.0
66-67	195393.0
68-69	191870.0
70-71	190112.0
72-73	182252.0
74-75	186703.0
76-77	180287.0
78-79	193103.0
80-81	198498.0
82-83	190834.0
84-85	192735.0
86-87	202426.0
88-89	219161.0
90-91	243211.0
92-93	243671.0
94-95	281550.0
96-97	412512.0
98-99	1459708.0
100-101	9677340.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.43314828558564
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.96043668644054	71.62895283951781
2	7.800777099875993	12.70483676634005
3	2.218391798078594	5.4195188474538325
4	0.8905797800295817	2.900908611491727
5	0.43123853197064327	1.755855566021183
6	0.2222402731150499	1.0858635069364146
7	0.14203579117775644	0.8096495151387101
8	0.08521049540478122	0.5551167126228611
9	0.057472728968198925	0.4212166734400155
>10	0.1873045396335598	2.3228895349008747
>50	0.003292431663050728	0.18216833271268695
>100	9.254335852505642E-4	0.12183386382807486
>500	4.046574418598559E-5	0.02629583368004612
>1k	5.394431282322851E-5	0.06489339591576623
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	5.7888889454913585E-6	0.0	5.7888889454913585E-6	0.0
4	0.0	7.525555629138767E-5	0.0	2.3155555781965434E-5	0.0
5	0.0	8.104444523687901E-5	0.0	3.473333367294815E-5	0.0
6	0.0	8.104444523687901E-5	0.0	3.473333367294815E-5	0.0
7	0.0	8.104444523687901E-5	0.0	4.052222261843951E-5	0.0
8	0.0	8.683333418237038E-5	0.0	4.052222261843951E-5	0.0
9	0.0	1.968222241467062E-4	0.0	4.052222261843951E-5	0.0
10-11	0.0	1.968222241467062E-4	0.0	4.631111156393087E-5	0.0
12-13	0.0	1.968222241467062E-4	0.0	4.920555603667655E-5	0.0
14-15	0.0	2.633944470198568E-4	0.0	5.210000050942223E-5	0.0
16-17	0.0	2.662888914926025E-4	0.0	6.367777840040494E-5	0.0
18-19	0.0	2.8944444727456794E-4	0.0	1.244611123280642E-4	0.0
20-21	0.0	3.3286111436575314E-4	0.0	3.06811114111042E-4	0.0
22-23	0.0	3.647000035659556E-4	0.0	7.322944516046569E-4	0.0
24-25	0.0	3.9364444829341233E-4	0.0	0.0017453500170656446	0.0
26-27	0.0	3.994333372389037E-4	0.0	0.00411300559577161	0.0
28-29	0.0	4.052222261843951E-4	0.0	0.025285866913906253	0.0
30-31	0.0	4.920555603667655E-4	0.0	0.03868714482271875	0.0
32-33	5.7888889454913585E-6	4.920555603667655E-4	0.0	0.0453559448879248	0.0
34-35	5.7888889454913585E-6	5.731000056036445E-4	0.0	0.05414058386270793	0.0
36-37	5.7888889454913585E-6	6.541444508405235E-4	0.0	0.06700060065511698	0.0
38-39	5.7888889454913585E-6	6.715111176769976E-4	0.0	0.09116342311359792	0.0
40-41	5.7888889454913585E-6	7.699222297503507E-4	0.0	0.13895070135862908	0.0
42-43	5.7888889454913585E-6	8.046555634232988E-4	0.0	0.1589686793321382	0.0
44-45	5.7888889454913585E-6	9.291166757513631E-4	0.0	0.18793917405984972	0.0
46-47	5.7888889454913585E-6	0.0010188444544064791	0.0	0.2225046299533786	0.0
48-49	5.7888889454913585E-6	0.0010275277878247161	0.0	0.2502710357804279	0.0
50-51	5.7888889454913585E-6	0.0011751444559347458	0.0	0.27816769160875077	0.0
52-53	5.7888889454913585E-6	0.0011751444559347458	0.0	0.31530920308302335	0.0
54-55	5.7888889454913585E-6	0.0011809333448802371	0.0	0.38706248156238876	0.0
56-57	5.7888889454913585E-6	0.0012040889006622027	0.0	0.4662081712251466	0.0
58-59	5.7888889454913585E-6	0.0012156666785531852	0.0	0.5499183998214243	0.0
60-61	5.7888889454913585E-6	0.0012330333453896593	0.0	0.6001977947574895	0.0
62-63	5.7888889454913585E-6	0.001244611123280642	0.0	0.6231970505379267	0.0
64-65	5.7888889454913585E-6	0.0014038055692816545	0.0	0.6447693451933003	0.0
66-67	5.7888889454913585E-6	0.0014067000137544	0.0	0.6536639730580478	0.0
68-69	5.7888889454913585E-6	0.0014182777916453828	0.0	0.6617221064701717	0.0
70-71	5.7888889454913585E-6	0.0014240666805908741	0.0	0.6692766065440379	0.0
72-73	5.7888889454913585E-6	0.0014240666805908741	0.0	0.674715267708327	0.0
74-75	5.7888889454913585E-6	0.0014472222363728397	0.0	0.6792595455305378	0.0
76-77	5.7888889454913585E-6	0.0014906389034640248	0.0	0.6831988844579446	0.0
78-79	5.7888889454913585E-6	0.0015166889037187359	0.0	0.6866635344918213	0.0
80-81	5.7888889454913585E-6	0.0015195833481914816	0.0	0.6883220511747045	0.0
82-83	5.7888889454913585E-6	0.0015369500150279557	0.0	0.6890109289592179	0.0
84-85	5.7888889454913585E-6	0.001560105570809921	0.0	0.6896650734100584	0.0
86-87	5.7888889454913585E-6	0.0015832611265918866	0.0	0.6898676845231506	0.0
88-89	5.7888889454913585E-6	0.0016816722386652396	0.0	0.6899545178573331	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	17850	0.0	46.68038	1
GTATCAA	31995	0.0	34.065193	1
TCAACGC	37700	0.0	28.19251	4
ATCAACG	38055	0.0	27.896719	3
CAACGCA	38765	0.0	27.353584	5
AACGCAG	39855	0.0	26.762125	6
TATCAAC	40830	0.0	26.36804	2
ACGCAGA	46450	0.0	22.854954	7
CGCAGAG	47100	0.0	22.513117	8
GTACATG	43935	0.0	21.332525	1
TACATGG	43825	0.0	20.949488	2
ACATGGG	43615	0.0	20.57304	3
CATGGGG	32715	0.0	18.501215	4
GCAGAGT	57170	0.0	18.460255	9
AGAGTAC	51165	0.0	17.5252	10-11
GAGTACT	35480	0.0	17.493483	12-13
CAGAGTA	55895	0.0	16.775246	10-11
AGTACTT	38965	0.0	15.806103	12-13
GTGGTAT	10475	0.0	15.329394	1
GTACTTT	40900	0.0	15.246938	14-15
>>END_MODULE