FastQCFastQC Report
Fri 27 May 2016
SRR2139885_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139885_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12131799
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA121300.046.8662451
GTATCAA217850.035.7044221
TCAACGC257700.029.2774964
ATCAACG260400.028.973933
CAACGCA265200.028.402445
AACGCAG273600.027.6216956
TATCAAC281150.027.1182252
ACGCAGA319800.023.5792667
CGCAGAG324900.023.1963318
GTACATG335650.022.4916271
TACATGG332700.022.3660132
ACATGGG340900.021.3019333
CATGGGG245400.019.5356774
GCAGAGT401350.018.7258479
AGAGTAC352450.017.5810610-11
GAGTACT227450.017.2716912-13
CAGAGTA388200.016.84102610-11
AGTACTT256750.015.243937512-13
GTACTTT262450.014.96922214-15
TATACTG64800.014.770785
Produced by FastQC (version 0.11.5)