##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139885_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12131799
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.191059215537614	33.0	31.0	34.0	30.0	34.0
2	32.36696272333559	34.0	31.0	34.0	30.0	34.0
3	32.479917281847484	34.0	31.0	34.0	31.0	34.0
4	36.04459280935993	37.0	35.0	37.0	35.0	37.0
5	35.85961142284009	37.0	35.0	37.0	35.0	37.0
6	35.89531412447569	37.0	35.0	37.0	35.0	37.0
7	35.86707305322154	37.0	35.0	37.0	35.0	37.0
8	35.84579756060911	37.0	35.0	37.0	35.0	37.0
9	37.65514215987258	39.0	37.0	39.0	35.0	39.0
10-11	37.51321972116419	39.0	37.0	39.0	35.0	39.0
12-13	37.53100496472122	39.0	37.0	39.0	35.0	39.0
14-15	38.80008678020465	40.0	38.0	41.0	35.5	41.0
16-17	38.74034448641953	40.0	38.0	41.0	35.0	41.0
18-19	38.799801043522066	40.0	38.0	41.0	35.0	41.0
20-21	38.79556129053121	40.0	38.0	41.0	35.0	41.0
22-23	38.75535677548919	40.0	38.0	41.0	35.0	41.0
24-25	38.73633486921972	40.0	38.0	41.0	35.0	41.0
26-27	38.630239476372296	40.0	38.0	41.0	34.5	41.0
28-29	38.54390545047429	40.0	38.0	41.0	34.0	41.0
30-31	38.39622094904027	40.0	38.0	41.0	34.0	41.0
32-33	38.305051304636606	40.0	38.0	41.0	34.0	41.0
34-35	38.25380939168126	40.0	38.0	41.0	34.0	41.0
36-37	38.15311089112038	40.0	38.0	41.0	34.0	41.0
38-39	38.11465943188742	40.0	38.0	41.0	33.5	41.0
40-41	38.10904705220423	40.0	38.0	41.0	33.0	41.0
42-43	38.06882187363777	40.0	37.5	41.0	33.0	41.0
44-45	37.95913217867201	40.0	37.0	41.0	33.0	41.0
46-47	37.897178939420996	40.0	37.0	41.0	33.0	41.0
48-49	37.87040874057905	40.0	37.0	41.0	33.0	41.0
50-51	37.78533846180774	40.0	37.0	41.0	33.0	41.0
52-53	37.64062410450721	39.0	37.0	41.0	33.0	41.0
54-55	37.45486764045562	39.0	36.0	40.0	32.0	41.0
56-57	37.324429310857376	39.0	36.0	40.0	32.0	41.0
58-59	37.09835510348277	39.0	35.5	40.0	31.5	41.0
60-61	36.9236064589705	39.0	35.0	40.0	31.0	41.0
62-63	36.7065409735806	38.0	35.0	40.0	31.0	41.0
64-65	36.38269978712395	38.0	35.0	40.0	31.0	41.0
66-67	36.3159576957364	37.5	35.0	40.0	31.0	41.0
68-69	36.086062429479426	37.0	34.5	40.0	31.0	41.0
70-71	35.777934423149546	36.5	34.0	39.0	30.0	41.0
72-73	35.46597272869403	36.0	34.0	39.0	30.0	40.0
74-75	35.064350898470934	35.5	34.0	38.5	30.0	40.0
76-77	33.000143473061826	34.0	31.0	36.0	27.0	39.0
78-79	34.240924067121256	35.0	33.0	37.0	29.0	39.0
80-81	34.27813498246255	35.0	34.0	37.0	30.0	39.0
82-83	33.99960359179525	35.0	33.0	36.0	29.0	38.5
84-85	33.74749380795929	35.0	33.0	36.0	29.0	37.0
86-87	33.54377489272724	35.0	33.0	35.5	29.0	37.0
88-89	33.31315089721837	35.0	33.0	35.0	29.0	36.5
90-91	33.157813494876066	35.0	33.0	35.0	29.0	36.0
92-93	33.09878965631915	35.0	33.0	35.0	29.0	36.0
94-95	33.08487452098663	34.5	33.0	35.0	29.0	36.0
96-97	33.138624040238	34.0	33.0	35.0	29.0	35.0
98-99	33.18716174733086	34.0	33.0	35.0	29.0	35.0
100-101	31.936327728141173	33.0	31.0	34.5	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	0.0
16	0.0
17	2.0
18	3.0
19	21.0
20	87.0
21	341.0
22	1494.0
23	4683.0
24	11987.0
25	25707.0
26	46399.0
27	75296.0
28	111689.0
29	154170.0
30	206332.0
31	268849.0
32	352840.0
33	476565.0
34	685229.0
35	1042951.0
36	1774136.0
37	2891106.0
38	2921872.0
39	1066314.0
40	13725.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.917031431199945	20.329878528320492	13.44862373667747	24.3044663038021
2	14.967351714366778	21.765815010248595	40.50317118740777	22.763662087976854
3	18.232085822798748	26.497355542315777	30.56913787151572	24.701420763369754
4	12.42660918246489	18.097986637923466	39.112416734305725	30.362987445305915
5	12.836850483424048	38.27512868667712	35.97660592928069	12.911414900618146
6	29.82354059772998	38.43639348129655	18.82579821838459	12.914267702588875
7	26.25357758182258	32.620177157582084	23.67405062299916	17.452194637596175
8	24.468285477480777	36.75699579903798	21.522732302731388	17.25198642074986
9	25.787439705909748	16.192916690089984	21.08023501238812	36.93940859161215
10-11	24.085089820201336	26.66222351982296	29.494430830993473	19.758255828982232
12-13	24.87780221957774	24.285930483168695	29.36875147701073	21.467515820242838
14-15	21.912502169917435	25.67533270830919	26.818308382648638	25.59385673912474
16-17	21.70139292444564	28.721460611345016	27.474277807754238	22.102868656455108
18-19	21.738097755093587	28.084366723068037	28.9316813934621	21.245854128376276
20-21	22.49833777006806	27.356376821796584	28.755094056742834	21.390191351392524
22-23	22.175008541784546	27.34979041558394	28.5872640621846	21.88793698044691
24-25	21.948209399557143	27.42639517622134	28.710537203840268	21.914858220381245
26-27	21.92042292351996	27.594183192942896	28.626306188761703	21.859087694775443
28-29	21.889589697019733	27.671798893750516	28.611458763312143	21.827152645917607
30-31	22.18376214120969	27.55492378913277	28.46879848695782	21.79251558269972
32-33	21.95206766036723	27.66562065298151	28.489000936843762	21.893310749807497
34-35	22.036360365879986	27.71504052090666	28.513802450731323	21.734796662482026
36-37	22.08696485463511	27.620083588871992	28.40758570497584	21.88536585151705
38-39	21.906033426257057	27.74180863284189	28.499550320167323	21.85260762073373
40-41	22.148110187715567	27.632603190556427	28.359556328430717	21.85973029329729
42-43	22.03337911659551	27.727882246546294	28.20745849092748	22.031280145930722
44-45	22.136281306396338	27.82461240163341	28.04491601114178	21.994190280828477
46-47	22.196728587954883	27.77455934382519	27.80891054014532	22.21980152807461
48-49	22.120269045204306	27.849084807061608	27.8487661296155	22.181880018118584
50-51	22.074180031082655	27.99366087422039	27.750865377022055	22.181293717674897
52-53	22.234510859679123	27.896109894524955	27.677892677441875	22.19148656835405
54-55	22.218454255614684	27.821285161189003	27.70027043073748	22.25999015245884
56-57	22.251867707093005	27.951990009720873	27.61065147222469	22.185490810961426
58-59	22.20585609733396	27.991500221386346	27.63641809828358	22.16622558299611
60-61	22.28536152154176	27.95421345703644	27.60444726692386	22.15597775449794
62-63	22.243953901918786	28.035973472668925	27.57759714753219	22.1424754778801
64-65	22.201223187588987	27.94228275161989	27.570909940509758	22.28558412028137
66-67	22.280505680023083	27.9867492227821	27.447991683054045	22.284753414140766
68-69	22.277357476993824	27.942977018259118	27.528334987341413	22.251330517405645
70-71	22.249876809734424	27.72866877633815	27.596673287866214	22.424781126061216
72-73	22.30947915469777	27.736483962196157	27.573902756154283	22.38013412695179
74-75	22.15242900699123	27.85815783579078	27.56510496271325	22.424308194504743
76-77	22.25402771917307	27.812343035525554	27.499583862384085	22.43404538291729
78-79	22.288118553153105	27.799595159350847	27.468403696040294	22.443882591455754
80-81	22.27465135599498	27.789725397298987	27.480884165997928	22.45473908070811
82-83	22.282582492102083	27.780539408096228	27.58512650715937	22.351751592642323
84-85	22.42001955774669	27.70718075075932	27.497427968371408	22.37537172312258
86-87	22.23877491396582	27.72978441336479	27.608052434812873	22.423388237856514
88-89	22.372008338690474	27.71355548081576	27.526528133146577	22.387908047347185
90-91	22.39143336331777	27.67588580797529	27.578523489467806	22.354157339239134
92-93	22.36780835970978	27.638044575958048	27.611744600710963	22.382402463621208
94-95	22.233434023626835	27.640505183618842	27.6876415319991	22.438419260755232
96-97	22.22840107244819	27.68087804946307	27.713608021242752	22.37711285684599
98-99	22.46489220840993	28.26780136008947	27.775270282430675	21.49203614906992
100-101	26.597628464347174	35.26191640919509	28.37006658078021	9.77038854567753
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1762.0
1	1696.5
2	2690.0
3	4026.0
4	5489.0
5	9063.5
6	12221.0
7	14380.0
8	15958.5
9	15918.5
10	14546.0
11	12975.5
12	13698.0
13	17762.0
14	27434.0
15	40632.0
16	51370.5
17	57837.5
18	59908.0
19	58332.0
20	55165.0
21	52466.5
22	52968.0
23	59405.5
24	71902.5
25	90129.0
26	113717.0
27	139200.0
28	166302.5
29	194794.0
30	221688.5
31	248447.5
32	276045.5
33	304343.0
34	331841.5
35	356404.0
36	383358.0
37	407119.5
38	424499.0
39	440165.5
40	456737.5
41	472943.5
42	482600.0
43	491089.0
44	500378.5
45	508821.0
46	515545.5
47	519517.0
48	519426.0
49	512928.5
50	503172.5
51	488854.5
52	468688.0
53	445976.5
54	421312.5
55	392318.5
56	360028.5
57	325730.0
58	289370.0
59	251780.0
60	213569.5
61	176392.5
62	141447.5
63	110962.5
64	85117.5
65	64836.5
66	49875.0
67	38733.0
68	29711.5
69	21985.0
70	16031.5
71	11566.0
72	8281.0
73	5819.0
74	4066.0
75	2848.5
76	1918.5
77	1263.0
78	842.5
79	592.5
80	437.0
81	328.0
82	247.5
83	170.5
84	115.0
85	84.5
86	66.5
87	51.5
88	32.0
89	19.5
90	14.0
91	12.0
92	11.0
93	8.0
94	5.0
95	3.0
96	2.0
97	2.0
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.00314050702620444
3	9.067080653083685E-5
4	1.648560118742488E-5
5	5.7699604155987084E-5
6	0.0
7	8.24280059371244E-6
8	4.4511123206047185E-4
9	1.0715640771826174E-4
10-11	5.7699604155987084E-5
12-13	2.637696189987981E-4
14-15	8.24280059371244E-6
16-17	4.6983963384160913E-4
18-19	1.2364200890568661E-5
20-21	7.830980405362045E-5
22-23	0.0
24-25	0.0
26-27	6.599616760254732E-5
28-29	8.25559248282073E-6
30-31	4.961225336663584E-5
32-33	3.3580002635822927E-4
34-35	0.0
36-37	3.34478126531152E-4
38-39	0.001057216604770355
40-41	0.0
42-43	0.0
44-45	2.898837159596475E-4
46-47	0.0
48-49	0.0
50-51	3.5794034503570075E-5
52-53	5.694359072996306E-4
54-55	0.0029126324842613984
56-57	0.0034759069105045367
58-59	0.004142211894452518
60-61	9.82891126047801E-4
62-63	0.0
64-65	6.575922164290712E-4
66-67	0.0
68-69	0.0
70-71	1.0043024820482187E-5
72-73	2.1362770824619737E-4
74-75	4.120394422695915E-5
76-77	0.0
78-79	0.0
80-81	5.358449170501352E-4
82-83	0.0
84-85	0.0
86-87	5.5904464636764375E-6
88-89	0.0
90-91	0.0011087400883977683
92-93	1.470584688589869E-4
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	310.0
20-21	1019.0
22-23	2104.0
24-25	4946.0
26-27	7360.0
28-29	15011.0
30-31	31445.0
32-33	38549.0
34-35	53107.0
36-37	82204.0
38-39	99091.0
40-41	98170.0
42-43	110792.0
44-45	129720.0
46-47	130237.0
48-49	123616.0
50-51	113549.0
52-53	109496.0
54-55	118333.0
56-57	124516.0
58-59	125304.0
60-61	124572.0
62-63	118287.0
64-65	120812.0
66-67	129230.0
68-69	130253.0
70-71	128941.0
72-73	122459.0
74-75	123818.0
76-77	120716.0
78-79	129443.0
80-81	133847.0
82-83	127153.0
84-85	127313.0
86-87	132376.0
88-89	143727.0
90-91	161426.0
92-93	158517.0
94-95	178597.0
96-97	267352.0
98-99	1014948.0
100-101	6919133.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.96517720974019
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.1298462231371	77.48028202458615
2	7.018649906579714	12.067189659845031
3	1.6525324027006207	4.261807225289898
4	0.5866935634779471	2.017408646087827
5	0.2317998773613311	0.9963358767281436
6	0.13027186234349594	0.6719306239080907
7	0.07715374998631014	0.46427750529783995
8	0.046455337688289404	0.3194833069369697
9	0.02504314262956486	0.1937554374635408
>10	0.09935102273318469	1.3075227103550802
>50	0.001671670930253616	0.09525058745759407
>100	4.5644465622478395E-4	0.06559666277424042
>500	4.488734639731637E-5	0.022971733813574702
>1k	2.990842955997663E-5	0.03618799945604162
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	8.242800593712442E-6	0.0
4	0.0	5.769960415598709E-5	0.0	8.242800593712442E-6	0.0
5	0.0	6.594240474969954E-5	0.0	1.6485601187424884E-5	0.0
6	0.0	6.594240474969954E-5	0.0	1.6485601187424884E-5	0.0
7	0.0	6.594240474969954E-5	0.0	1.6485601187424884E-5	0.0
8	0.0	8.242800593712441E-5	0.0	3.297120237484977E-5	0.0
9	0.0	1.566132112805364E-4	0.0	4.1214002968562206E-5	4.1214002968562206E-5
10-11	0.0	1.566132112805364E-4	0.0	5.769960415598709E-5	5.357820385913087E-5
12-13	0.0	1.566132112805364E-4	0.0	1.0303500742140551E-4	6.594240474969954E-5
14-15	0.0	2.3079841662394833E-4	0.0	1.1127780801511796E-4	7.418520534341197E-5
16-17	0.0	2.472840178113732E-4	0.0	1.607346115773926E-4	7.418520534341197E-5
18-19	0.0	2.7613381988936676E-4	0.0	2.0194861454595479E-4	7.418520534341197E-5
20-21	0.0	3.17347822857929E-4	0.0	2.1431281543652346E-4	8.654940623398063E-5
22-23	0.0	3.709260267170598E-4	0.0	2.7201241959251055E-4	1.1127780801511796E-4
24-25	0.0	4.203828302793345E-4	0.0	3.132264225610727E-4	1.1539920831197418E-4
26-27	0.0	4.4511123206047185E-4	0.0	4.038972290919096E-4	1.236420089056866E-4
28-29	0.0	4.4923263235732805E-4	0.0	9.72650470058068E-4	1.236420089056866E-4
30-31	0.0	5.440248391850211E-4	0.0	0.0022090705591149345	1.236420089056866E-4
32-33	0.0	5.687532409661585E-4	0.0	0.00415437149923107	1.3188480949939907E-4
34-35	0.0	6.55302647200139E-4	0.0	0.006507691068735972	1.3188480949939907E-4
36-37	0.0	7.253664522466948E-4	0.0	0.010357078945999684	1.3600620979625528E-4
38-39	0.0	7.418520534341196E-4	0.0	0.016580393394252574	1.4424901038996774E-4
40-41	0.0	8.160372587775317E-4	0.0	0.023388946684659052	1.566132112805364E-4
42-43	0.0	8.613726620429501E-4	0.0	0.029002293888977224	1.6485601187424883E-4
44-45	0.0	9.767718703549244E-4	0.0	0.03524209393841754	1.6485601187424883E-4
46-47	0.0	0.001067442676885761	0.0	0.04229793124663539	1.6485601187424883E-4
48-49	0.0	0.0010715640771826174	0.0	0.049419710959602944	1.6485601187424883E-4
50-51	0.0	0.0011787204849008793	0.0	0.05623238565030628	1.7309881246796126E-4
52-53	0.0	0.001211691687275729	0.0	0.06306566734249389	1.7309881246796126E-4
54-55	0.0	0.001215813087572585	0.0	0.06970524322072927	1.7309881246796126E-4
56-57	0.0	0.0012281772884631538	0.0	0.07619232728798095	1.7309881246796126E-4
58-59	0.0	0.0012364200890568663	0.0	0.08267941135523263	1.7309881246796126E-4
60-61	0.0	0.0012570270905411472	0.0	0.0889810324091258	1.7309881246796126E-4
62-63	0.0	0.0012982410935097094	0.0	0.0951754970553007	1.7309881246796126E-4
64-65	0.0	0.0014589757050871021	0.0	0.10120922708989821	1.7309881246796126E-4
66-67	0.0	0.0014713399059776708	0.0	0.10729653532835484	1.7309881246796126E-4
68-69	0.0	0.0015043111083525205	0.0	0.11354045677809202	1.7309881246796126E-4
70-71	0.0	0.001533160910430514	0.0	0.11940520940051842	1.7309881246796126E-4
72-73	0.0	0.0015414037110242265	0.0	0.1245652025721824	1.7309881246796126E-4
74-75	0.0	0.0015578893122116513	0.0	0.12914407830198965	1.7309881246796126E-4
76-77	0.0	0.0015908605145865012	0.0	0.13293164517480055	1.7309881246796126E-4
78-79	0.0	0.001607346115773926	0.0	0.13639362142415978	1.7309881246796126E-4
80-81	0.0	0.001607346115773926	0.0	0.13798036053844942	1.7309881246796126E-4
82-83	0.0	0.001607346115773926	0.0	0.1385862063820873	1.854630133585299E-4
84-85	0.0	0.0016155889163676383	0.0	0.13922914482839682	1.9782721424909858E-4
86-87	0.0	0.0016403173181487758	0.0	0.13944757904413022	1.9782721424909858E-4
88-89	0.0	0.0017145025234921877	0.0	0.13950115724798937	2.0607001484281102E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12130	0.0	46.866245	1
GTATCAA	21785	0.0	35.704422	1
TCAACGC	25770	0.0	29.277496	4
ATCAACG	26040	0.0	28.97393	3
CAACGCA	26520	0.0	28.40244	5
AACGCAG	27360	0.0	27.621695	6
TATCAAC	28115	0.0	27.118225	2
ACGCAGA	31980	0.0	23.579266	7
CGCAGAG	32490	0.0	23.196331	8
GTACATG	33565	0.0	22.491627	1
TACATGG	33270	0.0	22.366013	2
ACATGGG	34090	0.0	21.301933	3
CATGGGG	24540	0.0	19.535677	4
GCAGAGT	40135	0.0	18.725847	9
AGAGTAC	35245	0.0	17.58106	10-11
GAGTACT	22745	0.0	17.27169	12-13
CAGAGTA	38820	0.0	16.841026	10-11
AGTACTT	25675	0.0	15.2439375	12-13
GTACTTT	26245	0.0	14.969222	14-15
TATACTG	6480	0.0	14.77078	5
>>END_MODULE