FastQCFastQC Report
Fri 27 May 2016
SRR2139884_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139884_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13632109
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA140950.046.3516351
GTATCAA248350.034.3137781
TCAACGC295800.028.0526074
ATCAACG297700.027.831723
CAACGCA303000.027.221945
AACGCAG311050.026.584036
TATCAAC316450.026.5369032
ACGCAGA367100.022.536387
CGCAGAG375450.022.0241368
GTACATG373100.021.9078751
TACATGG372600.021.4926822
ACATGGG373900.020.9519733
CATGGGG284000.019.576034
GCAGAGT455300.018.1432369
GAGTACT262700.017.63869312-13
AGAGTAC398750.017.37095310-11
CAGAGTA432450.016.95128810-11
GTGGTAT77050.016.721921
ATGGGGG183500.015.5099765
CTTATAC94300.015.464271
Produced by FastQC (version 0.11.5)